Restriction map for
pFN21AB3134
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# NO CUT ENZYMES ( 81 enzymes) #
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AatII AccI AccII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AspEI AviII
AvrII BbeI BbrPI BcgI BciVI
BfrI BglI BlnI BsaAI BsiEI
BsiWI BsmBI BspDI BspLU11I BsrBI
BssHII Bst1107I BstUI BstZ17I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoT22I FauI FseI
FspI HaeII HpaI KasI KspI
MfeI MluI MunI MvnI NarI
NdeI NotI NruI NsiI PacI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
XbaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 24 GGTACC G^GTACC
AccIII 2408 TCCGGA T^CCGGA
AcyI 188 GGCGTC G[GA]^CG[CT]C
AocI 1297 CCTGAGG CC^T[ACGT]AGG
AseI 2071 ATTAAT AT^TAAT
AsnI 2071 ATTAAT AT^TAAT
Asp718I 24 GGTACC G^GTACC
AspI 1717 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 497 TGGCCA TGG^CCA
BsaHI 188 GGCGTC G[GA]^CG[CT]C
BsaI 1411 GAGACC GGTCTC(1/5)
BseAI 2408 TCCGGA T^CCGGA
Bsp1407I 1158 TGTACA T^GTACA
BspEI 2408 TCCGGA T^CCGGA
BsrFI 1106 GCCGGC [GA]^CCGG[CT]
BsrGI 1158 TGTACA T^GTACA
BssSI 1846 CACGAG CACGAG(-5/-1)
BstBI 2759 TTCGAA TT^CGAA
BstEII 121 GGTGACC G^GT[ACGT]ACC
BstPI 121 GGTGACC G^GT[ACGT]ACC
BstXI 488 CCAAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1297 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1106 GCCGGC [GA]^CCGG[CT]
DraI 2215 TTTAAA TTT^AAA
DraIII 2515 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 2458 CTCTTC CTCTTC(1/4)
Eco81I 1297 CCTGAGG CC^T[ACGT]AGG
EcoO65I 121 GGTGACC G^GT[ACGT]ACC
EcoRI 287 GAATTC G^AATTC
Hin1I 188 GGCGTC G[GA]^CG[CT]C
KpnI 24 GGTACC GGTAC^C
Ksp632I 2458 CTCTTC CTCTTC(1/4)
MluNI 497 TGGCCA TGG^CCA
MroI 2408 TCCGGA T^CCGGA
MscI 497 TGGCCA TGG^CCA
NaeI 1106 GCCGGC GCC^GGC
NgoMIV 1106 GCCGGC G^CCGGC
NspI 2005 GCATGT [GA]CATG^[CT]
NspV 2759 TTCGAA TT^CGAA
PaeR7I 1543 CTCGAG C^TCGAG
PflFI 1717 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2071 ATTAAT AT^TAAT
ScaI 1210 AGTACT AGT^ACT
SfuI 2759 TTCGAA TT^CGAA
SmaI 3239 CCCGGG CCC^GGG
SspBI 1158 TGTACA T^GTACA
StuI 3183 AGGCCT AGG^CCT
Tth111I 1717 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1543 CTCGAG C^TCGAG
XmaCI 3239 CCCGGG C^CCGGG
XmaI 3239 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AvaI 1543 CTCGAG C^[CT]CG[GA]G
3239 CCCGGG
BamHI 1492 GGATCC G^GATCC
2198 GGATCC
BanI 24 GGTACC G^G[CT][GA]CC
1113 GGCACC
BlpI 394 GCTGAGC GC^T[ACGT]AGC
3247 GCTGAGC
Bpu1102I 394 GCTGAGC GC^T[ACGT]AGC
3247 GCTGAGC
BsaWI 329 ACCGGA [AT]^CCGG[AT]
2408 TCCGGA
BsmI 635 GCATTC GAATGC(1/-1)
1637 GCATTC
BsoBI 1543 CTCGAG C^[CT]CG[GA]G
3239 CCCGGG
BspHI 1205 TCATGA T^CATGA
3665 TCATGA
BsrDI 3255 CATTGC GCAATG(2/0)
2930 GCAATG
BstAPI 2005 GCATGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2530 GCAGAAACTGC
CelII 394 GCTGAGC GC^T[ACGT]AGC
3247 GCTGAGC
EaeI 497 TGGCCA [CT]^GGCC[GA]
1706 TGGCCG
EcoRV 133 GATATC GAT^ATC
2056 GATATC
HincII 2125 GTCAAC GT[CT]^[GA]AC
3278 GTCAAC
HindII 2125 GTCAAC GT[CT]^[GA]AC
3278 GTCAAC
HindIII 424 AAGCTT A^AGCTT
1693 AAGCTT
NheI 2902 GCTAGC G^CTAGC
2910 GCTAGC
PshAI 1750 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3445 GACAGCAGTC
RcaI 1205 TCATGA T^CATGA
3665 TCATGA
SacI 1888 GAGCTC GAGCT^C
3250 GAGCTC
SspI 1230 AATATT AAT^ATT
2693 AATATT
XcmI 1445 CCACGGATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1743 CCACAGGGACTTTGG
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# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BanII BbsI BbvCI BbvI BclI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaBI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EcoNI
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI