Restriction map for
pFN21AB3134
(Linear) (Six-base) MAPSORT of: KIBB3134.seq Check: 6683 from: 1 to: 3690
KIBB3134.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1692 3690
Size: 1692 1998
Acc65I G'GTAC_C
Cuts at: 0 24 3690
Size: 24 3666
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 3690
Size: 307 3383
AleI CACnn'nnGTG
Cuts at: 0 3409 3690
Size: 3409 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1366 1398 2708 2740 3010 3042 3690
Size: 1366 32 1310 32 270 32 648
Fragments arranged by size:
1366 1310 648 270 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 742 2536 3369 3690
Size: 742 1794 833 321
Fragments arranged by size:
1794 833 742 321
ApoI r'AATT_y
Cuts at: 0 225 287 2348 2755 3690
Size: 225 62 2061 407 935
Fragments arranged by size:
2061 935 407 225 62
AseI AT'TA_AT
Cuts at: 0 2072 3690
Size: 2072 1618
AvaI C'yCGr_G
Cuts at: 0 1543 3239 3690
Size: 1543 1696 451
Fragments arranged by size:
1696 1543 451
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2586 2619 3690
Size: 2586 33 1071
Fragments arranged by size:
2586 1071 33
BamHI G'GATC_C
Cuts at: 0 1492 2198 3690
Size: 1492 706 1492
Fragments arranged by size:
1492 1492 706
BanI G'GyrC_C
Cuts at: 0 24 1113 3690
Size: 24 1089 2577
Fragments arranged by size:
2577 1089 24
BanII G_rGCy'C
Cuts at: 0 1283 1311 1892 3254 3690
Size: 1283 28 581 1362 436
Fragments arranged by size:
1362 1283 581 436 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 803 1679 1965 2261 3326 3690
Size: 803 876 286 296 1065 364
Fragments arranged by size:
1065 876 803 364 296 286
BbvCI CC'TCA_GC
Cuts at: 0 1343 1478 1660 2858 3260 3690
Size: 1343 135 182 1198 402 430
Fragments arranged by size:
1343 1198 430 402 182 135
BclI T'GATC_A
Cuts at: 0 87 146 1484 3690
Size: 87 59 1338 2206
Fragments arranged by size:
2206 1338 87 59
BglII A'GATC_T
Cuts at: 0 3059 3114 3510 3690
Size: 3059 55 396 180
Fragments arranged by size:
3059 396 180 55
BlpI GC'TnA_GC
Cuts at: 0 395 3248 3690
Size: 395 2853 442
Fragments arranged by size:
2853 442 395
Bme1580I G_kGCm'C
Cuts at: 0 1832 3690
Size: 1832 1858
BmgBI CAC'GTC
Cuts at: 0 1602 1784 3690
Size: 1602 182 1906
Fragments arranged by size:
1906 1602 182
BmrI ACTGGGnnnn_n'
Cuts at: 0 189 2481 3690
Size: 189 2292 1209
Fragments arranged by size:
2292 1209 189
BmtI G_CTAG'C
Cuts at: 0 2906 2914 3690
Size: 2906 8 776
Fragments arranged by size:
2906 776 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3059 3690
Size: 3059 631
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2122 3358 3391 3690
Size: 2122 1236 33 299
Fragments arranged by size:
2122 1236 299 33
Bpu10I CC'TnA_GC
Cuts at: 0 383 1343 1478 1660 2858 3034 3260 3358
Size: 383 960 135 182 1198 176 226 98
Cuts at: 3358 3690
Size: 332
Fragments arranged by size:
1198 960 383 332 226 182 176 135
98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 448 1237 1447 2010 2017 3139 3690
Size: 148 300 789 210 563 7 1122 551
Fragments arranged by size:
1122 789 563 551 300 210 148 7
BsaI GGTCTCn'nnnn_
Cuts at: 0 1405 3690
Size: 1405 2285
BsaBI GATnn'nnATC
Cuts at: 0 148 872 3310 3690
Size: 148 724 2438 380
Fragments arranged by size:
2438 724 380 148
BsaHI Gr'CG_yC
Cuts at: 0 189 3690
Size: 189 3501
BsaWI w'CCGG_w
Cuts at: 0 329 2408 3690
Size: 329 2079 1282
Fragments arranged by size:
2079 1282 329
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1366 1396 2187 2217 2708 2738 3010 3040
Size: 1366 30 791 30 491 30 272 30
Cuts at: 3040 3331 3361 3690
Size: 291 30 329
Fragments arranged by size:
1366 791 491 329 291 272 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1315 1360 1507 1580 3565 3690
Size: 1315 45 147 73 1985 125
Fragments arranged by size:
1985 1315 147 125 73 45
BseYI C'CCAG_C
Cuts at: 0 1865 2319 3690
Size: 1865 454 1371
Fragments arranged by size:
1865 1371 454
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1033 1363 2497 2830 3690
Size: 1033 330 1134 333 860
Fragments arranged by size:
1134 1033 860 333 330
BsiHKAI G_wGCw'C
Cuts at: 0 1892 2114 3254 3690
Size: 1892 222 1140 436
Fragments arranged by size:
1892 1140 436 222
BsmI GAATG_Cn'
Cuts at: 0 635 1637 3690
Size: 635 1002 2053
Fragments arranged by size:
2053 1002 635
Bsp1286I G_dGCh'C
Cuts at: 0 1283 1311 1832 1892 2114 3254 3690
Size: 1283 28 521 60 222 1140 436
Fragments arranged by size:
1283 1140 521 436 222 60 28
BspEI T'CCGG_A
Cuts at: 0 2408 3690
Size: 2408 1282
BspHI T'CATG_A
Cuts at: 0 1205 3665 3690
Size: 1205 2460 25
Fragments arranged by size:
2460 1205 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 36 390 1385 1692 2810 3690
Size: 36 354 995 307 1118 880
Fragments arranged by size:
1118 995 880 354 307 36
BsrDI GCAATG_nn'
Cuts at: 0 2937 3254 3690
Size: 2937 317 436
Fragments arranged by size:
2937 436 317
BsrFI r'CCGG_y
Cuts at: 0 1106 3690
Size: 1106 2584
BsrGI T'GTAC_A
Cuts at: 0 1158 3690
Size: 1158 2532
BssSI C'ACGA_G
Cuts at: 0 1846 3690
Size: 1846 1844
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2011 2536 3690
Size: 2011 525 1154
Fragments arranged by size:
2011 1154 525
BstBI TT'CG_AA
Cuts at: 0 2760 3690
Size: 2760 930
BstEII G'GTnAC_C
Cuts at: 0 121 3690
Size: 121 3569
BstXI CCAn_nnnn'nTGG
Cuts at: 0 495 3690
Size: 495 3195
BstYI r'GATC_y
Cuts at: 0 910 1492 1983 2198 2935 3059 3114 3510
Size: 910 582 491 215 737 124 55 396
Cuts at: 3510 3690
Size: 180
Fragments arranged by size:
910 737 582 491 396 215 180 124
55
Bsu36I CC'TnA_GG
Cuts at: 0 1298 3690
Size: 1298 2392
BtgI C'CryG_G
Cuts at: 0 1445 1505 2051 2492 2684 3497 3690
Size: 1445 60 546 441 192 813 193
Fragments arranged by size:
1445 813 546 441 193 192 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3658 3690
Size: 3658 32
BtsI GCAGTG_nn'
Cuts at: 0 3193 3690
Size: 3193 497
DraI TTT'AAA
Cuts at: 0 2217 3690
Size: 2217 1473
DraIII CAC_nnn'GTG
Cuts at: 0 2520 3690
Size: 2520 1170
EaeI y'GGCC_r
Cuts at: 0 497 1706 3690
Size: 497 1209 1984
Fragments arranged by size:
1984 1209 497
EarI CTCTTCn'nnn_
Cuts at: 0 2464 3690
Size: 2464 1226
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 499 3690
Size: 499 3191
EcoICRI GAG'CTC
Cuts at: 0 1890 3252 3690
Size: 1890 1362 438
Fragments arranged by size:
1890 1362 438
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 2122 3358 3391 3690
Size: 307 1815 1236 33 299
Fragments arranged by size:
1815 1236 307 299 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1121 1173 1253 3690
Size: 1121 52 80 2437
Fragments arranged by size:
2437 1121 80 52
EcoO109I rG'GnC_Cy
Cuts at: 0 445 1010 1166 1795 1971 3690
Size: 445 565 156 629 176 1719
Fragments arranged by size:
1719 629 565 445 176 156
EcoRI G'AATT_C
Cuts at: 0 287 3690
Size: 287 3403
EcoRV GAT'ATC
Cuts at: 0 135 2058 3690
Size: 135 1923 1632
Fragments arranged by size:
1923 1632 135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 337 3690
Size: 337 3353
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 231 263 2941 2973 3690
Size: 231 32 2678 32 717
Fragments arranged by size:
2678 717 231 32 32
HincII GTy'rAC
Cuts at: 0 2127 3280 3690
Size: 2127 1153 410
Fragments arranged by size:
2127 1153 410
HindIII A'AGCT_T
Cuts at: 0 424 1693 3690
Size: 424 1269 1997
Fragments arranged by size:
1997 1269 424
KpnI G_GTAC'C
Cuts at: 0 28 3690
Size: 28 3662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 2728 3467 3690
Size: 179 2549 739 223
Fragments arranged by size:
2549 739 223 179
MscI TGG'CCA
Cuts at: 0 499 3690
Size: 499 3191
MslI CAynn'nnrTG
Cuts at: 0 866 1604 3409 3690
Size: 866 738 1805 281
Fragments arranged by size:
1805 866 738 281
MspA1I CmG'CkG
Cuts at: 0 217 1086 1387 1731 2002 3336 3366 3690
Size: 217 869 301 344 271 1334 30 324
Fragments arranged by size:
1334 869 344 324 301 271 217 30
NaeI GCC'GGC
Cuts at: 0 1108 3690
Size: 1108 2582
NcoI C'CATG_G
Cuts at: 0 2051 2492 2684 3497 3690
Size: 2051 441 192 813 193
Fragments arranged by size:
2051 813 441 193 192
NgoMIV G'CCGG_C
Cuts at: 0 1106 3690
Size: 1106 2584
NheI G'CTAG_C
Cuts at: 0 2902 2910 3690
Size: 2902 8 780
Fragments arranged by size:
2902 780 8
NspI r_CATG'y
Cuts at: 0 2009 3690
Size: 2009 1681
PasI CC'CwG_GG
Cuts at: 0 1799 3690
Size: 1799 1891
PflMI CCAn_nnn'nTGG
Cuts at: 0 2052 2493 2498 2584 3690
Size: 2052 441 5 86 1106
Fragments arranged by size:
2052 1106 441 86 5
PfoI T'CCnGG_A
Cuts at: 0 563 1427 3314 3690
Size: 563 864 1887 376
Fragments arranged by size:
1887 864 563 376
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1366 1398 2708 2740 3010 3042 3690
Size: 1366 32 1310 32 270 32 648
Fragments arranged by size:
1366 1310 648 270 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 445 1010 1795 3690
Size: 445 565 785 1895
Fragments arranged by size:
1895 785 565 445
PshAI GACnn'nnGTC
Cuts at: 0 1754 3449 3690
Size: 1754 1695 241
Fragments arranged by size:
1754 1695 241
PsiI TTA'TAA
Cuts at: 0 97 3690
Size: 97 3593
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 252 284 3690
Size: 252 32 3406
Fragments arranged by size:
3406 252 32
PstI C_TGCA'G
Cuts at: 0 579 1064 1703 1967 2066 2532 3690
Size: 579 485 639 264 99 466 1158
Fragments arranged by size:
1158 639 579 485 466 264 99
PvuII CAG'CTG
Cuts at: 0 1086 1731 2002 3336 3366 3690
Size: 1086 645 271 1334 30 324
Fragments arranged by size:
1334 1086 645 324 271 30
SacI G_AGCT'C
Cuts at: 0 1892 3254 3690
Size: 1892 1362 436
Fragments arranged by size:
1892 1362 436
SanDI GG'GwC_CC
Cuts at: 0 445 1795 3690
Size: 445 1350 1895
Fragments arranged by size:
1895 1350 445
ScaI AGT'ACT
Cuts at: 0 1212 3690
Size: 1212 2478
SfcI C'TryA_G
Cuts at: 0 575 763 1060 1081 1699 1963 2062 2528
Size: 575 188 297 21 618 264 99 466
Cuts at: 2528 3433 3690
Size: 905 257
Fragments arranged by size:
905 618 575 466 297 264 257 188
99 21
SmaI CCC'GGG
Cuts at: 0 3241 3690
Size: 3241 449
SmlI C'TyrA_G
Cuts at: 0 127 427 1216 1462 1543 1989 2032 3118
Size: 127 300 789 246 81 446 43 1086
Cuts at: 3118 3690
Size: 572
Fragments arranged by size:
1086 789 572 446 300 246 127 81
43
SspI AAT'ATT
Cuts at: 0 1232 2695 3690
Size: 1232 1463 995
Fragments arranged by size:
1463 1232 995
StuI AGG'CCT
Cuts at: 0 3185 3690
Size: 3185 505
StyI C'CwwG_G
Cuts at: 0 803 843 2015 2051 2492 2684 2738 3497
Size: 803 40 1172 36 441 192 54 759
Cuts at: 3497 3690
Size: 193
Fragments arranged by size:
1172 803 759 441 193 192 54 40
36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 109 1733 2353 3490 3690
Size: 109 1624 620 1137 200
Fragments arranged by size:
1624 1137 620 200 109
TatI w'GTAC_w
Cuts at: 0 309 1158 1210 2261 3690
Size: 309 849 52 1051 1429
Fragments arranged by size:
1429 1051 849 309 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1762 1794 2187 2219 3690
Size: 1762 32 393 32 1471
Fragments arranged by size:
1762 1471 393 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1720 3690
Size: 1720 1970
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1452 1750 3690
Size: 1452 298 1940
Fragments arranged by size:
1940 1452 298
XhoI C'TCGA_G
Cuts at: 0 1543 3690
Size: 1543 2147
XmaI C'CCGG_G
Cuts at: 0 3239 3690
Size: 3239 451
XmnI GAAnn'nnTTC
Cuts at: 0 287 320 719 2723 3512 3690
Size: 287 33 399 2004 789 178
Fragments arranged by size:
2004 789 399 287 178 33
Enzymes that do cut:
AarI Acc65I AcuI AleI AloI AlwNI ApoI AseI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BclI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI Hin4I HincII HindIII KpnI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NheI NspI PasI PflMI
PfoI PpiI PpuMI PshAI PsiI PsrI PstI PvuII
SacI SanDI ScaI SfcI SmaI SmlI SspI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI ApaI ApaLI AscI AsiSI AvrII BbeI BcgI
BciVI BfrBI BglI BsaAI BsiEI BsiWI BsmBI BsrBI
BssHII BstZ17I ClaI CspCI DrdI EagI FseI FspI
FspAI HaeII HpaI KasI MfeI MluI NarI NdeI
NotI NruI NsiI PacI PciI PmeI PmlI PspOMI
PspXI PvuI RsrII SacII SalI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SwaI XbaI ZraI