################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MspA1I MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcyI 76 GGCGCC G[GA]^CG[CT]C AlwNI 43 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 502 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 567 GAGCAC G[AT]GC[AT]^C BanI 76 GGCGCC G^G[CT][GA]CC BanII 85 GGGCTC G[GA]GC[CT]^C BbeI 76 GGCGCC GGCGC^C BbrPI 232 CACGTG CAC^GTG BbsI 199 GTCTTC GAAGAC(2/6) BclI 492 TGATCA T^GATCA BpuAI 199 GTCTTC GAAGAC(2/6) BsaAI 232 CACGTG [CT]AC^GT[GA] BsaHI 76 GGCGCC G[GA]^CG[CT]C BsgI 340 CTGCAC GTGCAG(16/14) BsiHKAI 567 GAGCAC G[AT]GC[AT]^C BsmFI 270 GTCCC GGGAC(10/14) BspMI 4 GCAGGT ACCTGC(4/8) BsrDI 597 CATTGC GCAATG(2/0) BstAPI 42 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 322 GGTGACC G^GT[ACGT]ACC BstPI 322 GGTGACC G^GT[ACGT]ACC BstXI 668 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DpnII 493 GATC ^GATC DsaI 149 CCATGG C^C[GA][CT]GG EcoO65I 322 GGTGACC G^GT[ACGT]ACC EcoT14I 149 CCATGG C^C[AT][AT]GG FbaI 492 TGATCA T^GATCA HgaI 642 GCGTC GACGC(5/10) Hin1I 76 GGCGCC G[GA]^CG[CT]C HinfI 507 GATTC G^A[ACGT]TC KasI 76 GGCGCC G^GCGCC MboI 493 GATC ^GATC MslI 228 CATGCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 76 GGCGCC GG^CGCC NcoI 149 CCATGG C^CATGG NdeII 493 GATC ^GATC PmaCI 232 CACGTG CAC^GTG PmlI 232 CACGTG CAC^GTG PshAI 147 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 541 GAAGAGC GCTCTTC(1/4) Sau3AI 493 GATC ^GATC SfcI 466 CTACAG C^T[GA][CT]AG SfoI 76 GGCGCC GGC^GCC SmlI 649 CTCAAG C^T[CT][GA]AG StyI 149 CCATGG C^C[AT][AT]GG TfiI 507 GATTC G^A[AT]TC Tsp509I 349 AATT ^AATT TspRI 624 CACTG CA[GC]TG[ACGT][ACGT]^ XmnI 502 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 60 CGCG CG^CG 475 CGCG AfaI 170 GTAC GT^AC 279 GTAC Aor51HI 116 AGCGCT AGC^GCT 545 AGCGCT BbvI 51 GCTGC GCAGC(8/12) 442 GCTGC BcnI 272 CCCGG CC^[GC]GG 319 CCCGG BmyI 85 GGGCTC G[AGT]GC[ACT]^C 567 GAGCAC BsaI 106 GAGACC GGTCTC(1/5) 145 GAGACC BsmAI 106 GAGAC GTCTC(1/5) 145 GAGAC Bsp1286I 85 GGGCTC G[AGT]GC[ACT]^C 567 GAGCAC BsrI 625 ACTGG ACTGG(1/-1) 483 CCAGT BstUI 60 CGCG CG^CG 475 CGCG DdeI 434 CTGAG C^T[ACGT]AG 602 CTCAG EarI 367 CTCTTC CTCTTC(1/4) 541 GAAGAG Eco47III 116 AGCGCT AGC^GCT 545 AGCGCT FauI 479 CCCGC CCCGC(4/6) 27 GCGGG HapII 273 CCGG C^CGG 320 CCGG HpaII 273 CCGG C^CGG 320 CCGG Ksp632I 367 CTCTTC CTCTTC(1/4) 541 GAAGAG MaeII 233 ACGT A^CGT 301 ACGT MaeIII 323 GTGAC ^GT[ACGT]AC 644 GTCAC MspI 273 CCGG C^CGG 320 CCGG MvnI 60 CGCG CG^CG 475 CGCG NciI 272 CCCGG CC^[GC]GG 319 CCCGG NspI 227 GCATGC [GA]CATG^[CT] 417 GCATGC PpuMI 245 AGGACCC [GA]G^G[AT]CC[CT] 581 GGGTCCT RsaI 170 GTAC GT^AC 279 GTAC SphI 227 GCATGC GCATG^C 417 GCATGC TseI 51 GCTGC G^C[AT]GC 442 GCTGC Tsp45I 323 GTGAC ^GT[GC]AC 644 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AciI AluI AvaII BsaJI BsiYI BslI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DraII EcoO109I EcoRII Fnu4HI FokI HaeII HaeIII HhaI HinP1I HphI ItaI MboII MnlI MvaI MwoI NlaIII NlaIV Sau96I ScrFI SfaNI TaqI TthHB8I