(Circular) (Six-base) MAPSORT of: pFN21AE0503.seq Check: 1718 from: 1 to: 5365 pFN21AE0503 5365 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3175 278 Size: 53 83 186 936 1639 2468 Fragments arranged by size: 2468 1639 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2708 1937 Size: 771 4594 Acc65I G'GTAC_C Cuts at: 2686 2686 Size: 5365 AclI AA'CG_TT Cuts at: 1563 3492 3865 1563 Size: 1929 373 3063 Fragments arranged by size: 3063 1929 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2333 3428 4812 5080 1632 Size: 701 1095 1384 268 1917 Fragments arranged by size: 1917 1384 1095 701 268 AfeI AGC'GCT Cuts at: 2012 2129 2012 Size: 117 5248 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5168 AflIII A'CryG_T Cuts at: 1203 4264 5241 1203 Size: 3061 977 1327 Fragments arranged by size: 3061 1327 977 AgeI A'CCGG_T Cuts at: 4196 4196 Size: 5365 AhdI GACnn_n'nnGTC Cuts at: 4094 4094 Size: 5365 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5365 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2640 1184 Size: 1456 3909 AlwNI CAG_nnn'CTG Cuts at: 2077 2095 2251 2290 2521 3108 4680 2077 Size: 18 156 39 231 587 1572 2762 Fragments arranged by size: 2762 1572 587 231 156 39 18 ApaLI G'TGCA_C Cuts at: 3422 4578 3422 Size: 1156 4209 ApoI r'AATT_y Cuts at: 1493 2674 2900 3052 5161 1493 Size: 1181 226 152 2109 1697 Fragments arranged by size: 2109 1697 1181 226 152 AseI AT'TA_AT Cuts at: 160 1032 3919 160 Size: 872 2887 1606 Fragments arranged by size: 2887 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5365 AvaI C'yCGr_G Cuts at: 1870 1943 2690 1870 Size: 73 747 4545 Fragments arranged by size: 4545 747 73 BamHI G'GATC_C Cuts at: 2391 2695 3062 2391 Size: 304 367 4694 Fragments arranged by size: 4694 367 304 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2021 2045 2177 2686 4141 Size: 325 217 627 234 24 132 509 1455 Cuts at: 4141 618 Size: 1842 Fragments arranged by size: 1842 1455 627 509 325 234 217 132 24 BanII G_rGCy'C Cuts at: 1391 2684 1391 Size: 1293 4072 BbeI G_GCGC'C Cuts at: 2025 2025 Size: 5365 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4394 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2167 2201 3556 3590 1903 Size: 34 230 34 1355 34 3678 Fragments arranged by size: 3678 1355 230 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3257 4467 3257 Size: 1210 4155 BclI T'GATC_A Cuts at: 1548 1548 Size: 5365 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2318 3976 136 Size: 107 122 71 631 605 646 1658 1525 Fragments arranged by size: 1658 1525 646 631 605 122 107 71 BglII A'GATC_T Cuts at: 5360 5360 Size: 5365 BlpI GC'TnA_GC Cuts at: 2147 2358 2782 2147 Size: 211 424 4730 Fragments arranged by size: 4730 424 211 Bme1580I G_kGCm'C Cuts at: 1790 2050 3426 4582 1790 Size: 260 1376 1156 2573 Fragments arranged by size: 2573 1376 1156 260 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5365 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2626 3096 4054 Size: 416 53 83 399 24 1209 470 958 Cuts at: 4054 442 Size: 1753 Fragments arranged by size: 1753 1209 958 470 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5365 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2228 2453 2492 4025 1239 Size: 989 225 39 1533 2579 Fragments arranged by size: 2579 1533 989 225 39 Bpu10I CC'TnA_GC Cuts at: 2279 2279 Size: 5365 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3490 4355 4653 3490 Size: 865 298 4202 Fragments arranged by size: 4202 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4028 882 Size: 617 2529 2219 Fragments arranged by size: 2529 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2500 3156 493 Size: 711 1296 656 2702 Fragments arranged by size: 2702 1296 711 656 BsaBI GATnn'nnATC Cuts at: 5359 5359 Size: 5365 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2022 3172 3554 4189 Size: 53 83 186 936 489 1150 382 635 Cuts at: 4189 275 Size: 1451 Fragments arranged by size: 1451 1150 936 635 489 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2388 2442 2553 3798 4196 4470 4617 5028 2388 Size: 54 111 1245 398 274 147 411 2725 Fragments arranged by size: 2725 1245 411 398 274 147 111 54 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5140 Fragments arranged by size: 5140 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 2414 1186 Size: 859 369 4137 Fragments arranged by size: 4137 859 369 BseYI C'CCAG_C Cuts at: 2111 2313 2577 4568 5064 2111 Size: 202 264 1991 496 2412 Fragments arranged by size: 2412 1991 496 264 202 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2216 1163 Size: 573 480 4312 Fragments arranged by size: 4312 573 480 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2815 3576 4604 1540 Size: 275 186 814 761 1028 2301 Fragments arranged by size: 2301 1028 814 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2280 2388 2468 3426 3511 4582 2280 Size: 108 80 958 85 1071 3063 Fragments arranged by size: 3063 1071 958 108 85 80 BsmI GAATG_Cn' Cuts at: 2876 2969 2876 Size: 93 5272 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2050 2280 2388 2468 2684 3426 3511 Size: 399 260 230 108 80 216 742 85 Cuts at: 3511 4582 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 260 230 216 108 85 80 BspEI T'CCGG_A Cuts at: 2553 5028 2553 Size: 2475 2890 BspHI T'CATG_A Cuts at: 3254 4984 3254 Size: 1730 3635 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2346 2547 2720 844 Size: 1502 201 173 3489 Fragments arranged by size: 3489 1502 201 173 BsrBI CCG'CTC Cuts at: 1394 1823 3252 1394 Size: 429 1429 3507 Fragments arranged by size: 3507 1429 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3860 4034 65 Size: 1183 2612 174 1396 Fragments arranged by size: 2612 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2325 4009 4196 1700 Size: 110 515 1684 187 2869 Fragments arranged by size: 2869 1684 515 187 110 BsrGI T'GTAC_A Cuts at: 96 2238 96 Size: 2142 3223 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5365 BssSI C'ACGA_G Cuts at: 3425 4437 3425 Size: 1012 4353 BstAPI GCAn_nnn'nTGC Cuts at: 794 2275 2290 794 Size: 1481 15 3869 Fragments arranged by size: 3869 1481 15 BstBI TT'CG_AA Cuts at: 2187 4169 2187 Size: 1982 3383 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5365 BstYI r'GATC_y Cuts at: 1972 2211 2391 2695 3062 3447 3464 4916 5002 Size: 239 180 304 367 385 17 1452 86 Cuts at: 5002 5014 5360 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 304 239 180 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3874 Fragments arranged by size: 3874 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2426 487 Size: 683 56 1200 3426 Fragments arranged by size: 3426 1200 683 56 BtsI GCAGTG_nn' Cuts at: 2881 3675 3695 2881 Size: 794 20 4551 Fragments arranged by size: 4551 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5365 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5330 DraI TTT'AAA Cuts at: 2669 2669 Size: 5365 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4372 809 Size: 507 3056 1802 Fragments arranged by size: 3056 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2812 3701 5330 Size: 54 1425 50 275 18 982 889 1629 Cuts at: 5330 8 Size: 43 Fragments arranged by size: 1629 1425 982 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2812 1537 Size: 275 1000 4090 Fragments arranged by size: 4090 1000 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3295 1353 Size: 1942 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2054 3952 4326 4472 387 Size: 1064 138 465 1898 374 146 1280 Fragments arranged by size: 1898 1280 1064 465 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2228 2333 2453 2492 3428 4025 4812 Size: 393 596 105 120 39 936 597 787 Cuts at: 4812 5080 1239 Size: 268 1524 Fragments arranged by size: 1524 936 787 597 596 393 268 120 105 39 EcoNI CCTnn'n_nnAGG Cuts at: 2099 2099 Size: 5365 EcoO109I rG'GnC_Cy Cuts at: 2609 2609 Size: 5365 EcoRI G'AATT_C Cuts at: 2674 3052 2674 Size: 378 4987 FspI TGC'GCA Cuts at: 3871 3871 Size: 5365 HaeII r_GCGC'y Cuts at: 1678 2014 2025 2131 2168 2251 2291 4512 1678 Size: 336 11 106 37 83 40 2221 2531 Fragments arranged by size: 2531 2221 336 106 83 40 37 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4006 4038 4080 4112 1373 Size: 32 135 32 2434 32 42 32 2626 Fragments arranged by size: 2626 2434 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2709 2955 669 Size: 988 281 771 246 3079 Fragments arranged by size: 3079 988 771 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5365 HpaI GTT'AAC Cuts at: 2955 2955 Size: 5365 KasI G'GCGC_C Cuts at: 2021 2021 Size: 5365 KpnI G_GTAC'C Cuts at: 2690 2690 Size: 5365 MfeI C'AATT_G Cuts at: 2964 2964 Size: 5365 MluI A'CGCG_T Cuts at: 5241 5241 Size: 5365 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4479 4663 1305 Size: 3174 184 2007 Fragments arranged by size: 3174 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3543 Fragments arranged by size: 3543 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3323 3682 3841 518 Size: 648 2157 359 159 2042 Fragments arranged by size: 2157 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2074 2407 2781 3458 4606 4851 1606 Size: 468 333 374 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 468 374 333 245 NaeI GCC'GGC Cuts at: 2327 2327 Size: 5365 NarI GG'CG_CC Cuts at: 2022 2022 Size: 5365 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3874 Fragments arranged by size: 3874 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5365 NgoMIV G'CCGG_C Cuts at: 2325 2325 Size: 5365 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5365 NotI GC'GGCC_GC Cuts at: 2812 2812 Size: 5365 NspI r_CATG'y Cuts at: 1132 1229 2723 4268 1132 Size: 97 1494 1545 2229 Fragments arranged by size: 2229 1545 1494 97 PasI CC'CwG_GG Cuts at: 1348 2596 1348 Size: 1248 4117 PciI A'CATG_T Cuts at: 4264 4264 Size: 5365 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5365 PfoI T'CCnGG_A Cuts at: 2430 2430 Size: 5365 PmeI GTTT'AAAC Cuts at: 2669 2669 Size: 5365 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3434 3466 4947 4979 3434 Size: 32 1481 32 3820 Fragments arranged by size: 3820 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2609 2609 Size: 5365 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5365 PsiI TTA'TAA Cuts at: 2935 2935 Size: 5365 PstI C_TGCA'G Cuts at: 830 2532 2565 2717 830 Size: 1702 33 152 3478 Fragments arranged by size: 3478 1702 152 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5365 PvuII CAG'CTG Cuts at: 2074 2074 Size: 5365 SalI G'TCGA_C Cuts at: 1936 2707 1936 Size: 771 4594 SbfI CC_TGCA'GG Cuts at: 2717 2717 Size: 5365 ScaI AGT'ACT Cuts at: 3613 5325 3613 Size: 1712 3653 SfcI C'TryA_G Cuts at: 826 1045 2258 2528 2561 2713 3848 4529 4720 Size: 219 1213 270 33 152 1135 681 191 Cuts at: 4720 826 Size: 1471 Fragments arranged by size: 1471 1213 1135 681 270 219 191 152 33 SfoI GGC'GCC Cuts at: 2023 2023 Size: 5365 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5365 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5365 SmaI CCC'GGG Cuts at: 1872 2692 1872 Size: 820 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3469 4370 4632 820 Size: 197 926 1526 901 262 1553 Fragments arranged by size: 1553 1526 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3156 493 Size: 2663 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5365 SphI G_CATG'C Cuts at: 1132 2723 1132 Size: 1591 3774 SspI AAT'ATT Cuts at: 5 52 3289 5 Size: 47 3237 2081 Fragments arranged by size: 3237 2081 47 StuI AGG'CCT Cuts at: 1517 2321 1517 Size: 804 4561 StyI C'CwwG_G Cuts at: 513 1065 2004 2025 2804 513 Size: 552 939 21 779 3074 Fragments arranged by size: 3074 939 779 552 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3392 3409 3562 3747 4192 325 Size: 1255 1812 17 153 185 445 1498 Fragments arranged by size: 1812 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2238 3611 5323 Size: 274 80 33 51 1443 261 1373 1712 Cuts at: 5323 96 Size: 138 Fragments arranged by size: 1712 1443 1373 274 261 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5325 5357 5325 Size: 32 5333 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5365 XbaI T'CTAG_A Cuts at: 2701 2701 Size: 5365 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5365 XmaI C'CCGG_G Cuts at: 1870 2690 1870 Size: 820 4545 XmnI GAAnn'nnTTC Cuts at: 3494 3494 Size: 5365 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3173 276 Size: 53 83 186 936 1639 2468 Fragments arranged by size: 2468 1639 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI