Restriction map for
pFN21AB6108
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BcgI BciVI BfrI BglII
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsoBI
BspDI BspEI BspHI BsrDI BssHII
BstBI BstEII BstPI BstYI Bsu36I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI Tth111I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 40 CGCG CG^CG
AflIII 715 ACATGT A^C[GA][CT]GT
Asp700I 1059 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 101 TGGCCA TGG^CCA
BbsI 839 GAAGAC GAAGAC(2/6)
BclI 1712 TGATCA T^GATCA
BcnI 1698 CCCGG CC^[GC]GG
BglI 7 GCCGTGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 839 GAAGAC GAAGAC(2/6)
BsaI 1004 GGTCTC GGTCTC(1/5)
BsmBI 1562 CGTCTC CGTCTC(1/5)
BspLU11I 715 ACATGT A^CATGT
BsrBI 1626 GAGCGG CCGCTC(-3/-3)
BssSI 1028 CTCGTG CACGAG(-5/-1)
Bst1107I 632 GTATAC GTA^TAC
BstAPI 1386 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 1542 CATCC GGATG(2/0)
BstUI 40 CGCG CG^CG
BstXI 104 CCAGCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 632 GTATAC GTA^TAC
DraIII 516 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1639 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1712 TGATCA T^GATCA
FokI 1542 CATCC GGATG(9/13)
HaeII 65 AGCGCC [GA]GCGC^[CT]
MaeII 98 ACGT A^CGT
MluNI 101 TGGCCA TGG^CCA
MscI 101 TGGCCA TGG^CCA
MseI 1789 TTAA T^TAA
MvnI 40 CGCG CG^CG
NciI 1698 CCCGG CC^[GC]GG
NcoI 1580 CCATGG C^CATGG
PstI 61 CTGCAG CTGCA^G
SphI 1395 GCATGC GCATG^C
StuI 1214 AGGCCT AGG^CCT
TaqI 1067 TCGA T^CGA
Tru9I 1789 TTAA T^TAA
TthHB8I 1067 TCGA T^CGA
XcmI 414 CCATCAGGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1059 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 475 GTAGAC GT^[AC][GT]AC
632 GTATAC
AlwI 447 GATCC GGATC(4/5)
1478 GGATC
AvaII 288 GGACC G^G[AT]CC
988 GGACC
BbvCI 637 CCTCAGC CCTCAGC(-5/-2)
1534 CCTCAGC
BfaI 52 CTAG C^TAG
1112 CTAG
BsmAI 1005 GTCTC GTCTC(1/5)
1563 GTCTC
BsmFI 1575 GTCCC GGGAC(10/14)
1606 GTCCC
BsmI 355 GAATGC GAATGC(1/-1)
488 GAATGC
BspMI 1125 ACCTGC ACCTGC(4/8)
1659 ACCTGC
BsrFI 938 ACCGGC [GA]^CCGG[CT]
1406 ACCGGC
Cfr10I 938 ACCGGC [GA]^CCGG[CT]
1406 ACCGGC
DraII 287 AGGACCC [GA]G^G[ACGT]CC[CT]
987 AGGACCT
DsaI 8 CCGTGG C^C[GA][CT]GG
1580 CCATGG
EarI 1813 CTCTTC CTCTTC(1/4)
1268 GAAGAG
EcoO109I 287 AGGACCC [GA]G^G[ACGT]CC[CT]
987 AGGACCT
FauI 1608 CCCGC CCCGC(4/6)
970 GCGGG
HindIII 787 AAGCTT A^AGCTT
1363 AAGCTT
Ksp632I 1813 CTCTTC CTCTTC(1/4)
1268 GAAGAG
MaeI 52 CTAG C^TAG
1112 CTAG
MslI 216 CACCATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1173 CACCCCTGTG
MspA1I 1136 CAGCTG C[AC]G^C[GT]G
1390 CAGCTG
NspI 715 ACATGT [GA]CATG^[CT]
1395 GCATGC
PflMI 140 CCAACGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
202 CCAAAGCATGG
PpuMI 287 AGGACCC [GA]G^G[AT]CC[CT]
987 AGGACCT
PvuII 1136 CAGCTG CAG^CTG
1390 CAGCTG
SacI 1157 GAGCTC GAGCT^C
1446 GAGCTC
SfaNI 89 GCATC GCATC(5/9)
1541 GCATC
SmlI 1346 CTCAAG C^T[CT][GA]AG
1826 CTCAAG
TfiI 212 GATTC G^A[AT]TC
1471 GAATC
Tsp45I 1106 GTGAC ^GT[GC]AC
1171 GTCAC
Van91I 140 CCAACGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
202 CCAAAGCATGG
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AspHI BanII BbvI BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsiHKAI BsiYI BslI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstNI Cac8I
CelII CfoI Cfr13I DdeI DpnII
EaeI EcoRII EcoT14I Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfcI SspBI StyI
TseI Tsp509I TspRI