(Circular) (Six-base) MAPSORT of: pFN21AB6108.seq Check: 195 from: 1 to: 6556 pFN21AB6108 6556 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3139 3673 3139 Size: 534 6022 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4366 278 Size: 53 83 186 936 2830 2468 Fragments arranged by size: 2830 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2481 2638 3899 1937 Size: 544 157 1261 4594 Fragments arranged by size: 4594 1261 544 157 Acc65I G'GTAC_C Cuts at: 3877 3877 Size: 6556 AclI AA'CG_TT Cuts at: 1563 4683 5056 1563 Size: 3120 373 3063 Fragments arranged by size: 3120 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3065 3356 3383 3805 4619 6003 6271 1632 Size: 1433 291 27 422 814 1384 268 1917 Fragments arranged by size: 1917 1433 1384 814 422 291 268 27 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6359 AflIII A'CryG_T Cuts at: 1203 2720 5455 6432 1203 Size: 1517 2735 977 1327 Fragments arranged by size: 2735 1517 1327 977 AgeI A'CCGG_T Cuts at: 5387 5387 Size: 6556 AhdI GACnn_n'nnGTC Cuts at: 5285 5285 Size: 6556 AleI CACnn'nnGTG Cuts at: 1166 3182 1166 Size: 2016 4540 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3417 1184 Size: 2233 4323 AlwNI CAG_nnn'CTG Cuts at: 2996 3067 3131 3397 4299 5871 2996 Size: 71 64 266 902 1572 3681 Fragments arranged by size: 3681 1572 902 266 71 64 ApaLI G'TGCA_C Cuts at: 4613 5769 4613 Size: 1156 5400 ApoI r'AATT_y Cuts at: 1493 2337 2439 2981 3865 4091 4243 6352 1493 Size: 844 102 542 884 226 152 2109 1697 Fragments arranged by size: 2109 1697 884 844 542 226 152 102 AseI AT'TA_AT Cuts at: 160 1032 5110 160 Size: 872 4078 1606 Fragments arranged by size: 4078 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6556 AvaI C'yCGr_G Cuts at: 1870 1943 3881 1870 Size: 73 1938 4545 Fragments arranged by size: 4545 1938 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3749 3782 3749 Size: 33 6523 BamHI G'GATC_C Cuts at: 3886 4253 3886 Size: 367 6189 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3877 5332 618 Size: 325 217 627 2090 1455 1842 Fragments arranged by size: 2090 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3166 3455 3704 3711 3875 1391 Size: 1775 289 249 7 164 4072 Fragments arranged by size: 4072 1775 289 249 164 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2851 928 Size: 971 952 4633 Fragments arranged by size: 4633 971 952 BbvCI CC'TCA_GC Cuts at: 2643 3540 2643 Size: 897 5659 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4747 4781 1903 Size: 34 2810 34 3678 Fragments arranged by size: 3678 2810 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4448 5658 4448 Size: 1210 5346 BclI T'GATC_A Cuts at: 1548 3717 1548 Size: 2169 4387 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2018 5167 136 Size: 107 122 71 631 605 346 3149 1525 Fragments arranged by size: 3149 1525 631 605 346 122 107 71 BglII A'GATC_T Cuts at: 6551 6551 Size: 6556 BlpI GC'TnA_GC Cuts at: 2237 3139 3207 3973 2237 Size: 902 68 766 4820 Fragments arranged by size: 4820 902 766 68 Bme1580I G_kGCm'C Cuts at: 1790 4617 5773 1790 Size: 2827 1156 2573 Fragments arranged by size: 2827 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2104 1318 Size: 786 5770 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2441 2610 4287 Size: 416 53 83 399 24 1024 169 1677 Cuts at: 4287 5245 442 Size: 958 1753 Fragments arranged by size: 1753 1677 1024 958 416 399 169 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6556 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2273 2451 3174 3357 3728 3734 5216 1239 Size: 1034 178 723 183 371 6 1482 2579 Fragments arranged by size: 2579 1482 1034 723 371 183 178 6 Bpu10I CC'TnA_GC Cuts at: 2643 3540 2643 Size: 897 5659 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3336 3816 4681 5546 5844 3336 Size: 480 865 865 298 4048 Fragments arranged by size: 4048 865 865 480 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3015 5219 882 Size: 617 1516 2204 2219 Fragments arranged by size: 2219 2204 1516 617 BsaAI yAC'GTr Cuts at: 493 1204 4347 493 Size: 711 3143 2702 Fragments arranged by size: 3143 2702 711 BsaBI GATnn'nnATC Cuts at: 6550 6550 Size: 6556 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4363 4745 5380 275 Size: 53 83 186 936 2830 382 635 1451 Fragments arranged by size: 2830 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4989 5387 5661 5808 6219 4989 Size: 398 274 147 411 5326 Fragments arranged by size: 5326 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3288 3318 3547 3577 1178 Size: 30 165 30 1885 30 229 30 4157 Fragments arranged by size: 4157 1885 229 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3003 3507 3674 1186 Size: 1817 504 167 4068 Fragments arranged by size: 4068 1817 504 167 BseYI C'CCAG_C Cuts at: 5759 6255 5759 Size: 496 6060 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5983 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4006 4767 5795 1540 Size: 275 186 2005 761 1028 2301 Fragments arranged by size: 2301 2005 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3166 3444 3455 4617 4702 5773 3166 Size: 278 11 1162 85 1071 3949 Fragments arranged by size: 3949 1162 1071 278 85 11 BsmI GAATG_Cn' Cuts at: 2366 2499 4067 4160 2366 Size: 133 1568 93 4762 Fragments arranged by size: 4762 1568 133 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3573 3573 Size: 6556 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3166 3444 3455 3704 3711 3875 4617 Size: 399 1376 278 11 249 7 164 742 Cuts at: 4617 4702 5773 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1376 1071 742 399 278 249 164 85 11 7 BspEI T'CCGG_A Cuts at: 6219 6219 Size: 6556 BspHI T'CATG_A Cuts at: 4445 6175 4445 Size: 1730 4826 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3139 3673 3911 844 Size: 2295 534 238 3489 Fragments arranged by size: 3489 2295 534 238 BsrBI CCG'CTC Cuts at: 1394 1823 3633 4443 1394 Size: 429 1810 810 3507 Fragments arranged by size: 3507 1810 810 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5051 5225 65 Size: 1183 3803 174 1396 Fragments arranged by size: 3803 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2943 3411 5200 5387 1700 Size: 110 1133 468 1789 187 2869 Fragments arranged by size: 2869 1789 1133 468 187 110 BsrGI T'GTAC_A Cuts at: 96 2124 3040 3379 96 Size: 2028 916 339 3273 Fragments arranged by size: 3273 2028 916 339 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6556 BssSI C'ACGA_G Cuts at: 3033 4616 5628 3033 Size: 1583 1012 3961 Fragments arranged by size: 3961 1583 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3397 794 Size: 2603 3953 BstBI TT'CG_AA Cuts at: 5360 5360 Size: 6556 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6556 BstXI CCAn_nnnn'nTGG Cuts at: 2116 2116 Size: 6556 BstYI r'GATC_y Cuts at: 1972 3886 4253 4638 4655 6107 6193 6205 6551 Size: 1914 367 385 17 1452 86 12 346 Cuts at: 6551 1972 Size: 1977 Fragments arranged by size: 1977 1914 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2639 2639 Size: 6556 BtgI C'CryG_G Cuts at: 513 1065 2004 2013 3585 513 Size: 552 939 9 1572 3484 Fragments arranged by size: 3484 1572 939 552 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5817 Fragments arranged by size: 5817 683 56 BtsI GCAGTG_nn' Cuts at: 3758 4072 4866 4886 3758 Size: 314 794 20 5428 Fragments arranged by size: 5428 794 314 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6556 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6521 DraI TTT'AAA Cuts at: 3860 3860 Size: 6556 DraIII CAC_nnn'GTG Cuts at: 2526 2526 Size: 6556 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5563 809 Size: 507 4247 1802 Fragments arranged by size: 4247 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2106 2134 3413 Size: 54 1425 50 275 18 276 28 1279 Cuts at: 3413 3423 4003 4892 6521 8 Size: 10 580 889 1629 43 Fragments arranged by size: 1629 1425 1279 889 580 276 275 54 50 43 28 18 10 EagI C'GGCC_G Cuts at: 1537 1812 4003 1537 Size: 275 2191 4090 Fragments arranged by size: 4090 2191 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3268 3824 4486 1353 Size: 1915 556 662 3423 Fragments arranged by size: 3423 1915 662 556 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5143 5517 5663 387 Size: 1064 138 3554 374 146 1280 Fragments arranged by size: 3554 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3164 3453 3164 Size: 289 6267 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2273 2451 3065 3174 3356 3357 3383 Size: 393 641 178 614 109 182 1 26 Cuts at: 3383 3728 3734 3805 4619 5216 6003 6271 1239 Size: 345 6 71 814 597 787 268 1524 Fragments arranged by size: 1524 814 787 641 614 597 393 345 268 182 178 109 71 26 6 1 EcoNI CCTnn'n_nnAGG Cuts at: 3648 3648 Size: 6556 EcoO109I rG'GnC_Cy Cuts at: 2293 2993 2293 Size: 700 5856 EcoRI G'AATT_C Cuts at: 3865 4243 3865 Size: 378 6178 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2217 2810 2217 Size: 593 5963 FspI TGC'GCA Cuts at: 5062 5062 Size: 6556 HaeII r_GCGC'y Cuts at: 1678 2074 5703 1678 Size: 396 3629 2531 Fragments arranged by size: 3629 2531 396 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2313 2345 3288 3320 3334 Size: 32 135 32 741 32 943 32 14 Cuts at: 3334 3366 5197 5229 5271 5303 1373 Size: 32 1831 32 42 32 2626 Fragments arranged by size: 2626 1831 943 741 135 42 32 32 32 32 32 32 32 14 HincII GTy'rAC Cuts at: 669 1657 1938 3900 4146 669 Size: 988 281 1962 246 3079 Fragments arranged by size: 3079 1962 988 281 246 HindIII A'AGCT_T Cuts at: 748 2792 3368 748 Size: 2044 576 3936 Fragments arranged by size: 3936 2044 576 HpaI GTT'AAC Cuts at: 4146 4146 Size: 6556 KpnI G_GTAC'C Cuts at: 3881 3881 Size: 6556 MfeI C'AATT_G Cuts at: 4155 4155 Size: 6556 MluI A'CGCG_T Cuts at: 6432 6432 Size: 6556 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2308 5670 5854 1305 Size: 1003 3362 184 2007 Fragments arranged by size: 3362 2007 1003 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2108 10 Size: 54 1425 343 276 4458 Fragments arranged by size: 4458 1425 343 276 54 MslI CAynn'nnrTG Cuts at: 518 1166 2225 3182 4514 4873 5032 518 Size: 648 1059 957 1332 359 159 2042 Fragments arranged by size: 2042 1332 1059 957 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3143 3397 3972 4649 5797 6042 1606 Size: 1537 254 575 677 1148 245 2120 Fragments arranged by size: 2120 1537 1148 677 575 254 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3585 513 Size: 552 939 1581 3484 Fragments arranged by size: 3484 1581 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6556 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6556 NotI GC'GGCC_GC Cuts at: 4003 4003 Size: 6556 NspI r_CATG'y Cuts at: 1132 1229 2724 3404 3914 5459 1132 Size: 97 1495 680 510 1545 2229 Fragments arranged by size: 2229 1545 1495 680 510 97 PasI CC'CwG_GG Cuts at: 1348 3092 1348 Size: 1744 4812 PciI A'CATG_T Cuts at: 2720 5455 2720 Size: 2735 3821 PflMI CCAn_nnn'nTGG Cuts at: 1108 2151 2213 1108 Size: 1043 62 5451 Fragments arranged by size: 5451 1043 62 PfoI T'CCnGG_A Cuts at: 2289 3190 3479 3750 2289 Size: 901 289 271 5095 Fragments arranged by size: 5095 901 289 271 PmeI GTTT'AAAC Cuts at: 3860 3860 Size: 6556 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4625 4657 6138 6170 4625 Size: 32 1481 32 5011 Fragments arranged by size: 5011 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2293 2993 2293 Size: 700 5856 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6556 PsiI TTA'TAA Cuts at: 4126 4126 Size: 6556 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2521 2553 2521 Size: 32 6524 PstI C_TGCA'G Cuts at: 830 2070 3908 830 Size: 1240 1838 3478 Fragments arranged by size: 3478 1838 1240 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6556 PvuII CAG'CTG Cuts at: 3143 3397 3143 Size: 254 6302 SacI G_AGCT'C Cuts at: 3166 3455 3166 Size: 289 6267 SalI G'TCGA_C Cuts at: 1936 3898 1936 Size: 1962 4594 SbfI CC_TGCA'GG Cuts at: 3908 3908 Size: 6556 ScaI AGT'ACT Cuts at: 4804 6516 4804 Size: 1712 4844 SfcI C'TryA_G Cuts at: 826 1045 2066 2308 3555 3904 5039 5720 5911 Size: 219 1021 242 1247 349 1135 681 191 Cuts at: 5911 826 Size: 1471 Fragments arranged by size: 1471 1247 1135 1021 681 349 242 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6556 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6556 SmaI CCC'GGG Cuts at: 1872 3883 1872 Size: 2011 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3351 3831 4660 5561 5823 820 Size: 197 926 1408 480 829 901 262 1553 Fragments arranged by size: 1553 1408 926 901 829 480 262 197 SnaBI TAC'GTA Cuts at: 493 4347 493 Size: 3854 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6556 SphI G_CATG'C Cuts at: 1132 3404 3914 1132 Size: 2272 510 3774 Fragments arranged by size: 3774 2272 510 SspI AAT'ATT Cuts at: 5 52 4480 5 Size: 47 4428 2081 Fragments arranged by size: 4428 2081 47 StuI AGG'CCT Cuts at: 1517 3221 1517 Size: 1704 4852 StyI C'CwwG_G Cuts at: 513 1065 2004 3420 3471 3585 3995 513 Size: 552 939 1416 51 114 410 3074 Fragments arranged by size: 3074 1416 939 552 410 114 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2934 3511 4583 4600 4753 4938 5383 Size: 1255 1354 577 1072 17 153 185 445 Cuts at: 5383 325 Size: 1498 Fragments arranged by size: 1498 1354 1255 1072 577 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2124 3040 3119 Size: 274 80 33 51 1443 147 916 79 Cuts at: 3119 3379 4802 6514 96 Size: 260 1423 1712 138 Fragments arranged by size: 1712 1443 1423 916 274 260 147 138 80 79 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6516 6548 6516 Size: 32 6524 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6556 XbaI T'CTAG_A Cuts at: 3892 3892 Size: 6556 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2426 2011 Size: 415 6141 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6556 XmaI C'CCGG_G Cuts at: 1870 3881 1870 Size: 2011 4545 XmnI GAAnn'nnTTC Cuts at: 3068 4685 3068 Size: 1617 4939 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4364 276 Size: 53 83 186 936 2830 2468 Fragments arranged by size: 2830 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI