Restriction map for
pFN21AB6108
(Linear) (Six-base) MAPSORT of: KIBB6108.seq Check: 5947 from: 1 to: 1851
KIBB6108.orf
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1134 1668 1851
Size: 1134 534 183
Fragments arranged by size:
1134 534 183
AccI GT'mk_AC
Cuts at: 0 476 633 1851
Size: 476 157 1218
Fragments arranged by size:
1218 476 157
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1060 1351 1378 1800 1851
Size: 1060 291 27 422 51
Fragments arranged by size:
1060 422 291 51 27
AflIII A'CryG_T
Cuts at: 0 715 1851
Size: 715 1136
AleI CACnn'nnGTG
Cuts at: 0 1177 1851
Size: 1177 674
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1412 1851
Size: 1412 439
AlwNI CAG_nnn'CTG
Cuts at: 0 991 1062 1126 1392 1851
Size: 991 71 64 266 459
Fragments arranged by size:
991 459 266 71 64
ApoI r'AATT_y
Cuts at: 0 332 434 976 1851
Size: 332 102 542 875
Fragments arranged by size:
875 542 332 102
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1744 1777 1851
Size: 1744 33 74
Fragments arranged by size:
1744 74 33
BanII G_rGCy'C
Cuts at: 0 1161 1450 1699 1706 1851
Size: 1161 289 249 7 145
Fragments arranged by size:
1161 289 249 145 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 846 1851
Size: 846 1005
BbvCI CC'TCA_GC
Cuts at: 0 638 1535 1851
Size: 638 897 316
Fragments arranged by size:
897 638 316
BclI T'GATC_A
Cuts at: 0 1712 1851
Size: 1712 139
BglI GCCn_nnn'nGGC
Cuts at: 0 13 1851
Size: 13 1838
BlpI GC'TnA_GC
Cuts at: 0 232 1134 1202 1851
Size: 232 902 68 649
Fragments arranged by size:
902 649 232 68
BmgBI CAC'GTC
Cuts at: 0 99 1851
Size: 99 1752
BmrI ACTGGGnnnn_n'
Cuts at: 0 436 605 1851
Size: 436 169 1246
Fragments arranged by size:
1246 436 169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 446 1169 1352 1723 1729 1851
Size: 268 178 723 183 371 6 122
Fragments arranged by size:
723 371 268 183 178 122 6
Bpu10I CC'TnA_GC
Cuts at: 0 638 1535 1851
Size: 638 897 316
Fragments arranged by size:
897 638 316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1331 1811 1851
Size: 1331 480 40
Fragments arranged by size:
1331 480 40
BsaI GGTCTCn'nnnn_
Cuts at: 0 1010 1851
Size: 1010 841
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1283 1313 1542 1572 1851
Size: 1283 30 229 30 279
Fragments arranged by size:
1283 279 229 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 998 1502 1669 1851
Size: 998 504 167 182
Fragments arranged by size:
998 504 182 167
BsiHKAI G_wGCw'C
Cuts at: 0 1161 1439 1450 1851
Size: 1161 278 11 401
Fragments arranged by size:
1161 401 278 11
BsmI GAATG_Cn'
Cuts at: 0 361 494 1851
Size: 361 133 1357
Fragments arranged by size:
1357 361 133
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1568 1851
Size: 1568 283
Bsp1286I G_dGCh'C
Cuts at: 0 1161 1439 1450 1699 1706 1851
Size: 1161 278 11 249 7 145
Fragments arranged by size:
1161 278 249 145 11 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1134 1668 1851
Size: 1134 534 183
Fragments arranged by size:
1134 534 183
BsrBI CCG'CTC
Cuts at: 0 1628 1851
Size: 1628 223
BsrFI r'CCGG_y
Cuts at: 0 938 1406 1851
Size: 938 468 445
Fragments arranged by size:
938 468 445
BsrGI T'GTAC_A
Cuts at: 0 119 1035 1374 1851
Size: 119 916 339 477
Fragments arranged by size:
916 477 339 119
BssSI C'ACGA_G
Cuts at: 0 1028 1851
Size: 1028 823
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1392 1851
Size: 1392 459
BstXI CCAn_nnnn'nTGG
Cuts at: 0 111 1851
Size: 111 1740
BstZ17I GTA'TAC
Cuts at: 0 634 1851
Size: 634 1217
BtgI C'CryG_G
Cuts at: 0 8 1580 1851
Size: 8 1572 271
Fragments arranged by size:
1572 271 8
BtsI GCAGTG_nn'
Cuts at: 0 1753 1851
Size: 1753 98
DraIII CAC_nnn'GTG
Cuts at: 0 521 1851
Size: 521 1330
EaeI y'GGCC_r
Cuts at: 0 101 129 1408 1418 1851
Size: 101 28 1279 10 433
Fragments arranged by size:
1279 433 101 28 10
EarI CTCTTCn'nnn_
Cuts at: 0 1263 1819 1851
Size: 1263 556 32
Fragments arranged by size:
1263 556 32
EcoICRI GAG'CTC
Cuts at: 0 1159 1448 1851
Size: 1159 289 403
Fragments arranged by size:
1159 403 289
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 446 1060 1169 1351 1352 1378 1723
Size: 268 178 614 109 182 1 26 345
Cuts at: 1723 1729 1800 1851
Size: 6 71 51
Fragments arranged by size:
614 345 268 182 178 109 71 51
26 6 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1643 1851
Size: 1643 208
EcoO109I rG'GnC_Cy
Cuts at: 0 288 988 1851
Size: 288 700 863
Fragments arranged by size:
863 700 288
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 212 805 1851
Size: 212 593 1046
Fragments arranged by size:
1046 593 212
HaeII r_GCGC'y
Cuts at: 0 69 1851
Size: 69 1782
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 308 340 1283 1315 1329 1361 1851
Size: 308 32 943 32 14 32 490
Fragments arranged by size:
943 490 308 32 32 32 14
HindIII A'AGCT_T
Cuts at: 0 787 1363 1851
Size: 787 576 488
Fragments arranged by size:
787 576 488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 1851
Size: 303 1548
MscI TGG'CCA
Cuts at: 0 103 1851
Size: 103 1748
MslI CAynn'nnrTG
Cuts at: 0 220 1177 1851
Size: 220 957 674
Fragments arranged by size:
957 674 220
MspA1I CmG'CkG
Cuts at: 0 1138 1392 1851
Size: 1138 254 459
Fragments arranged by size:
1138 459 254
NcoI C'CATG_G
Cuts at: 0 1580 1851
Size: 1580 271
NspI r_CATG'y
Cuts at: 0 719 1399 1851
Size: 719 680 452
Fragments arranged by size:
719 680 452
PasI CC'CwG_GG
Cuts at: 0 1087 1851
Size: 1087 764
PciI A'CATG_T
Cuts at: 0 715 1851
Size: 715 1136
PflMI CCAn_nnn'nTGG
Cuts at: 0 146 208 1851
Size: 146 62 1643
Fragments arranged by size:
1643 146 62
PfoI T'CCnGG_A
Cuts at: 0 284 1185 1474 1745 1851
Size: 284 901 289 271 106
Fragments arranged by size:
901 289 284 271 106
PpuMI rG'GwC_Cy
Cuts at: 0 288 988 1851
Size: 288 700 863
Fragments arranged by size:
863 700 288
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 516 548 1851
Size: 516 32 1303
Fragments arranged by size:
1303 516 32
PstI C_TGCA'G
Cuts at: 0 65 1851
Size: 65 1786
PvuII CAG'CTG
Cuts at: 0 1138 1392 1851
Size: 1138 254 459
Fragments arranged by size:
1138 459 254
SacI G_AGCT'C
Cuts at: 0 1161 1450 1851
Size: 1161 289 401
Fragments arranged by size:
1161 401 289
SfcI C'TryA_G
Cuts at: 0 61 303 1550 1851
Size: 61 242 1247 301
Fragments arranged by size:
1247 301 242 61
SmlI C'TyrA_G
Cuts at: 0 1346 1826 1851
Size: 1346 480 25
Fragments arranged by size:
1346 480 25
SphI G_CATG'C
Cuts at: 0 1399 1851
Size: 1399 452
StuI AGG'CCT
Cuts at: 0 1216 1851
Size: 1216 635
StyI C'CwwG_G
Cuts at: 0 1415 1466 1580 1851
Size: 1415 51 114 271
Fragments arranged by size:
1415 271 114 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 929 1506 1851
Size: 929 577 345
Fragments arranged by size:
929 577 345
TatI w'GTAC_w
Cuts at: 0 119 1035 1114 1374 1851
Size: 119 916 79 260 477
Fragments arranged by size:
916 477 260 119 79
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 421 1851
Size: 421 1430
XmnI GAAnn'nnTTC
Cuts at: 0 1063 1851
Size: 1063 788
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI ApoI
BaeI BanII BbsI BbvCI BclI BglI BlpI BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI
BstAPI BstXI BstZ17I BtgI BtsI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII
MmeI MscI MslI MspA1I NcoI NspI PasI PciI
PflMI PfoI PpuMI PsrI PstI PvuII SacI SfcI
SmlI SphI StuI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BamHI
BanI BbeI BcgI BciVI BfrBI BglII Bme1580I BmtI
BplI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiWI BspEI BspHI BsrDI BssHII BstBI BstEII BstYI
Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EciI
EcoRI EcoRV FseI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI TstI Tth111I
XbaI XhoI XmaI ZraI