Restriction map for
pFN21AB6402
##################################
# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccII AciI AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BcgI BciVI BcnI
BfaI BfrI BglI BlnI BlpI
BpmI Bpu1102I BsaBI BsaHI BsaI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT14I EcoT22I FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MseI
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I SspBI StyI
SwaI TaqI Tru9I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 499 GTAGAC GT^[AC][GT]AC
AccIII 455 TCCGGA T^CCGGA
AcsI 259 GAATTC [GA]^AATT[CT]
AfaI 636 GTAC GT^AC
AlwI 822 GGATC GGATC(4/5)
AlwNI 490 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 727 GGGCCC GGGCC^C
ApoI 259 GAATTC [GA]^AATT[CT]
Asp700I 309 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 434 GTGCTC G[AT]GC[AT]^C
AvaII 86 GGACC G^G[AT]CC
BanII 727 GGGCCC G[GA]GC[CT]^C
BbsI 814 GAAGAC GAAGAC(2/6)
BclI 63 TGATCA T^GATCA
BglII 346 AGATCT A^GATCT
BpuAI 814 GAAGAC GAAGAC(2/6)
BseAI 455 TCCGGA T^CCGGA
BseRI 141 GAGGAG GAGGAG(10/8)
BsgI 181 CTGCAC GTGCAG(16/14)
BsiHKAI 434 GTGCTC G[AT]GC[AT]^C
BsmAI 242 GAGAC GTCTC(1/5)
BspEI 455 TCCGGA T^CCGGA
BspHI 756 TCATGA T^CATGA
BsrFI 667 ACCGGC [GA]^CCGG[CT]
BstXI 344 CCAGATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 346 AGATCT [GA]^GATC[CT]
Cac8I 982 GCCAGC GC[ACGT]^[ACGT]GC
Cfr10I 667 ACCGGC [GA]^CCGG[CT]
DraIII 479 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 702 CCTGATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 259 GAATTC G^AATTC
FbaI 63 TGATCA T^GATCA
MaeIII 376 GTGAC ^GT[ACGT]AC
MflI 346 AGATCT [GA]^GATC[CT]
MroI 455 TCCGGA T^CCGGA
MslI 165 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 900 CAGCTG C[AC]G^C[GT]G
NdeI 608 CATATG CA^TATG
PvuII 900 CAGCTG CAG^CTG
RcaI 756 TCATGA T^CATGA
RsaI 636 GTAC GT^AC
SfcI 804 CTGTAG C^T[GA][CT]AG
SmlI 515 CTCAAG C^T[CT][GA]AG
SnaBI 931 TACGTA TAC^GTA
SspI 881 AATATT AAT^ATT
StuI 582 AGGCCT AGG^CCT
Tsp45I 376 GTGAC ^GT[GC]AC
XhoII 346 AGATCT [GA]^GATC[CT]
XmnI 309 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BalI 224 TGGCCA TGG^CCA
941 TGGCCA
BbvCI 44 CCTCAGC CCTCAGC(-5/-2)
974 CCTCAGC
BmyI 434 GTGCTC G[AGT]GC[ACT]^C
727 GGGCCC
BsaAI 373 TACGTG [CT]AC^GT[GA]
931 TACGTA
BsaJI 33 CCTGGG C^C[ACGT][ACGT]GG
298 CCCAGG
BsaWI 455 TCCGGA [AT]^CCGG[AT]
950 ACCGGA
Bsp1286I 434 GTGCTC G[AGT]GC[ACT]^C
727 GGGCCC
BspMI 267 GCAGGT ACCTGC(4/8)
751 GCAGGT
BsrDI 521 GCAATG GCAATG(2/0)
533 GCAATG
EaeI 224 TGGCCA [CT]^GGCC[GA]
941 TGGCCA
MaeII 374 ACGT A^CGT
932 ACGT
MluNI 224 TGGCCA TGG^CCA
941 TGGCCA
MscI 224 TGGCCA TGG^CCA
941 TGGCCA
SfaNI 102 GATGC GCATC(5/9)
748 GATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AluI BbvI BsiYI BslI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MboI MboII MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
TfiI TseI Tsp509I TspRI