Restriction map for
pFN21AB6452
(Circular) (Six-base) MAPSORT of: pFN21AB6452.seq Check: 220 from: 1 to: 5755
pFN21AB6452 5755 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3565 278
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2040 3098 1937
Size: 103 1058 4594
Fragments arranged by size:
4594 1058 103
Acc65I G'GTAC_C
Cuts at: 3076 3076
Size: 5755
AclI AA'CG_TT
Cuts at: 1563 3882 4255 1563
Size: 2319 373 3063
Fragments arranged by size:
3063 2319 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2237 2801 3818 5202 5470 1632
Size: 605 564 1017 1384 268 1917
Fragments arranged by size:
1917 1384 1017 605 564 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 2649 4654 5631 1203
Size: 1446 2005 977 1327
Fragments arranged by size:
2005 1446 1327 977
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 2320 2882 4484 2320
Size: 562 1602 3591
Fragments arranged by size:
3591 1602 562
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5755
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2688 2720 2688
Size: 32 5723
AlwNI CAG_nnn'CTG
Cuts at: 2389 2944 3498 5070 2389
Size: 555 554 1572 3074
Fragments arranged by size:
3074 1572 555 554
ApaI G_GGCC'C
Cuts at: 2035 2035
Size: 5755
ApaLI G'TGCA_C
Cuts at: 3812 4968 3812
Size: 1156 4599
ApoI r'AATT_y
Cuts at: 1493 2546 3064 3290 3442 5551 1493
Size: 1053 518 226 152 2109 1697
Fragments arranged by size:
2109 1697 1053 518 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2178 4309 160
Size: 872 1146 2131 1606
Fragments arranged by size:
2131 1606 1146 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 2034 2098 3080 1870
Size: 73 91 64 982 4545
Fragments arranged by size:
4545 982 91 73 64
BamHI G'GATC_C
Cuts at: 3085 3452 3085
Size: 367 5388
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3076 4531 618
Size: 325 217 627 1289 1455 1842
Fragments arranged by size:
1842 1455 1289 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2035 2613 3074 1391
Size: 644 578 461 4072
Fragments arranged by size:
4072 644 578 461
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4784
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3946 3980 1903
Size: 34 50 34 1925 34 3678
Fragments arranged by size:
3678 1925 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3647 4857 3647
Size: 1210 4545
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5755
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2957 4366 136
Size: 107 122 71 631 605 1285 1409 1525
Fragments arranged by size:
1525 1409 1285 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2902 5750 2902
Size: 2848 2907
BlpI GC'TnA_GC
Cuts at: 3172 3172
Size: 5755
Bme1580I G_kGCm'C
Cuts at: 1790 2035 3816 4972 1790
Size: 245 1781 1156 2573
Fragments arranged by size:
2573 1781 1156 245
BmgBI CAC'GTC
Cuts at: 1318 2095 1318
Size: 777 4978
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2086 3486 4444
Size: 416 53 83 399 24 669 1400 958
Cuts at: 4444 442
Size: 1753
Fragments arranged by size:
1753 1400 958 669 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2282 4415 1239
Size: 1043 2133 2579
Fragments arranged by size:
2579 2133 1043
Bpu10I CC'TnA_GC
Cuts at: 2562 2562
Size: 5755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2572 3880 4745 5043 2572
Size: 1308 865 298 3284
Fragments arranged by size:
3284 1308 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3044 4418 882
Size: 617 1545 1374 2219
Fragments arranged by size:
2219 1545 1374 617
BsaAI yAC'GTr
Cuts at: 493 1204 3546 493
Size: 711 2342 2702
Fragments arranged by size:
2702 2342 711
BsaBI GATnn'nnATC
Cuts at: 5749 5749
Size: 5755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3562 3944 4579 275
Size: 53 83 186 936 2029 382 635 1451
Fragments arranged by size:
2029 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2406 4188 4586 4860 5007 5418 2406
Size: 1782 398 274 147 411 2743
Fragments arranged by size:
2743 1782 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2595 2625 1178
Size: 30 165 30 1192 30 4308
Fragments arranged by size:
4308 1192 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2077 2603 1186
Size: 891 526 4338
Fragments arranged by size:
4338 891 526
BseYI C'CCAG_C
Cuts at: 2103 2847 3003 4958 5454 2103
Size: 744 156 1955 496 2404
Fragments arranged by size:
2404 1955 744 496 156
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3033 1163
Size: 573 1297 3885
Fragments arranged by size:
3885 1297 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2019 3205 3966 4994 1540
Size: 275 186 18 1186 761 1028 2301
Fragments arranged by size:
2301 1186 1028 761 275 186 18
BsiHKAI G_wGCw'C
Cuts at: 2613 2970 3816 3901 4972 2613
Size: 357 846 85 1071 3396
Fragments arranged by size:
3396 1071 846 357 85
BsmI GAATG_Cn'
Cuts at: 3266 3359 3266
Size: 93 5662
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2613 2970 3074 3816 3901 4972
Size: 399 245 578 357 104 742 85 1071
Cuts at: 4972 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 578 399 357 245 104
85
BspEI T'CCGG_A
Cuts at: 5418 5418
Size: 5755
BspHI T'CATG_A
Cuts at: 2493 3644 5374 2493
Size: 1151 1730 2874
Fragments arranged by size:
2874 1730 1151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3110 844
Size: 2266 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3642 1394
Size: 429 1819 3507
Fragments arranged by size:
3507 1819 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2739 4250 4424 65
Size: 1183 1491 1511 174 1396
Fragments arranged by size:
1511 1491 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4399 4586 1700
Size: 110 2589 187 2869
Fragments arranged by size:
2869 2589 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5755
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5755
BssSI C'ACGA_G
Cuts at: 2615 3051 3815 4827 2615
Size: 436 764 1012 3543
Fragments arranged by size:
3543 1012 764 436
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3020 794
Size: 2226 3529
BstBI TT'CG_AA
Cuts at: 2134 2689 4559 2134
Size: 555 1870 3330
Fragments arranged by size:
3330 1870 555
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5755
BstXI CCAn_nnnn'nTGG
Cuts at: 2752 2752
Size: 5755
BstYI r'GATC_y
Cuts at: 1972 2385 2803 2902 3085 3452 3837 3854 5306
Size: 413 418 99 183 367 385 17 1452
Cuts at: 5306 5392 5404 5750 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 418 413 385 367 346 183
99 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2892 2892
Size: 5755
BtgI C'CryG_G
Cuts at: 513 1065 2019 2707 2961 513
Size: 552 954 688 254 3307
Fragments arranged by size:
3307 954 688 552 254
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5016
Fragments arranged by size:
5016 683 56
BtsI GCAGTG_nn'
Cuts at: 3271 4065 4085 3271
Size: 794 20 4941
Fragments arranged by size:
4941 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4762 809
Size: 507 3446 1802
Fragments arranged by size:
3446 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 3202 4091
Size: 54 1425 50 275 18 186 1186 889
Cuts at: 4091 5720 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1186 889 275 186 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2016 3202 1537
Size: 275 204 1186 4090
Fragments arranged by size:
4090 1186 275 204
EarI CTCTTCn'nnn_
Cuts at: 1353 3685 1353
Size: 2332 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4342 4716 4862 387
Size: 1064 138 2753 374 146 1280
Fragments arranged by size:
2753 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2611 2611
Size: 5755
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2282 2801 3818 4415 5202 5470
Size: 393 605 45 519 1017 597 787 268
Cuts at: 5470 1239
Size: 1524
Fragments arranged by size:
1524 1017 787 605 597 519 393 268
45
EcoO109I rG'GnC_Cy
Cuts at: 2031 2314 2896 2031
Size: 283 582 4890
Fragments arranged by size:
4890 582 283
EcoRI G'AATT_C
Cuts at: 2546 3064 3442 2546
Size: 518 378 4859
Fragments arranged by size:
4859 518 378
EcoRV GAT'ATC
Cuts at: 2404 2821 2404
Size: 417 5338
FspI TGC'GCA
Cuts at: 4261 4261
Size: 5755
HaeII r_GCGC'y
Cuts at: 1678 4902 1678
Size: 3224 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2306 2338 2595 2627 2868
Size: 32 135 32 734 32 257 32 241
Cuts at: 2868 2900 4396 4428 4470 4502 1373
Size: 32 1496 32 42 32 2626
Fragments arranged by size:
2626 1496 734 257 241 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3099 3345 669
Size: 988 281 1161 246 3079
Fragments arranged by size:
3079 1161 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KpnI G_GTAC'C
Cuts at: 3080 3080
Size: 5755
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4869 5053 1305
Size: 3564 184 2007
Fragments arranged by size:
3564 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3933
Fragments arranged by size:
3933 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2472 3713 4072 4231 518
Size: 648 1306 1241 359 159 2042
Fragments arranged by size:
2042 1306 1241 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2021 3171 3848 4996 5241 1606
Size: 415 1150 677 1148 245 2120
Fragments arranged by size:
2120 1150 1148 677 415 245
NcoI C'CATG_G
Cuts at: 513 1065 2707 2961 513
Size: 552 1642 254 3307
Fragments arranged by size:
3307 1642 552 254
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 2653 3113 4658 1132
Size: 97 1424 460 1545 2229
Fragments arranged by size:
2229 1545 1424 460 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5755
PciI A'CATG_T
Cuts at: 2649 4654 2649
Size: 2005 3750
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5755
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 5337 5369 3824
Size: 32 1481 32 4210
Fragments arranged by size:
4210 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2314 2314
Size: 5755
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5755
PsiI TTA'TAA
Cuts at: 3325 3325
Size: 5755
PspOMI G'GGCC_C
Cuts at: 2031 2031
Size: 5755
PstI C_TGCA'G
Cuts at: 830 3107 830
Size: 2277 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
SacI G_AGCT'C
Cuts at: 2613 2613
Size: 5755
SacII CC_GC'GG
Cuts at: 2022 2022
Size: 5755
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 2075 4003 5715 2075
Size: 1928 1712 2115
Fragments arranged by size:
2115 1928 1712
SfcI C'TryA_G
Cuts at: 826 1045 3103 4238 4919 5110 826
Size: 219 2058 1135 681 191 1471
Fragments arranged by size:
2058 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5755
SmaI CCC'GGG
Cuts at: 1872 2036 3082 1872
Size: 164 1046 4545
Fragments arranged by size:
4545 1046 164
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2587 3859 4760 5022 820
Size: 197 926 644 1272 901 262 1553
Fragments arranged by size:
1553 1272 926 901 644 262 197
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 3113 1132
Size: 1981 3774
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5755
StyI C'CwwG_G
Cuts at: 513 1065 2707 2745 2961 3194 513
Size: 552 1642 38 216 233 3074
Fragments arranged by size:
3074 1642 552 233 216 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2985 3782 3799 3952 4137 4582 325
Size: 1255 1405 797 17 153 185 445 1498
Fragments arranged by size:
1498 1405 1255 797 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2073 2568 4001
Size: 274 80 33 51 1443 96 495 1433
Cuts at: 4001 5713 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1433 495 274 138 96 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5715 5747 5715
Size: 32 5723
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5755
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2959 2959
Size: 5755
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 2034 3080 1870
Size: 164 1046 4545
Fragments arranged by size:
4545 1046 164
XmnI GAAnn'nnTTC
Cuts at: 3884 3884
Size: 5755
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3563 276
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI PvuII RsrII SanDI SapI
SexAI SfiI SfoI SrfI SwaI