################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AvaI AvrII BalI BamHI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaI BseAI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoO65I EcoRI EcoT22I FbaI FseI HincII HindII HindIII HpaI Ksp632I MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NcoI NdeI NheI NlaIII NruI NsiI NspI NspV PacI PaeR7I PflFI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 869 GGTACC G^GTACC AgeI 837 ACCGGT A^CCGGT AlwNI 455 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 653 GGGCCC GGGCC^C Asp718I 869 GGTACC G^GTACC AspHI 821 GAGCAC G[AT]GC[AT]^C AviII 395 TGCGCA TGC^GCA BbrPI 721 CACGTG CAC^GTG BbsI 238 GAAGAC GAAGAC(2/6) BpuAI 238 GAAGAC GAAGAC(2/6) BsaAI 721 CACGTG [CT]AC^GT[GA] BsaWI 837 ACCGGT [AT]^CCGG[AT] BsgI 452 GTGCAG GTGCAG(16/14) BsiHKAI 821 GAGCAC G[AT]GC[AT]^C Bsp1407I 351 TGTACA T^GTACA BsrBI 600 GAGCGG CCGCTC(-3/-3) BsrGI 351 TGTACA T^GTACA BstAPI 454 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DpnII 517 GATC ^GATC EcoRV 983 GATATC GAT^ATC FspI 395 TGCGCA TGC^GCA KpnI 869 GGTACC GGTAC^C MboI 517 GATC ^GATC NaeI 229 GCCGGC GCC^GGC NdeII 517 GATC ^GATC NgoMIV 229 GCCGGC G^CCGGC PflMI 907 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 837 ACCGGT A^CCGGT PmaCI 721 CACGTG CAC^GTG PmlI 721 CACGTG CAC^GTG PstI 512 CTGCAG CTGCA^G PvuII 664 CAGCTG CAG^CTG Sau3AI 517 GATC ^GATC SspBI 351 TGTACA T^GTACA TfiI 660 GAATC G^A[AT]TC Tsp45I 469 GTGAC ^GT[GC]AC Van91I 907 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 95 CCAAGAGCCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AluI 459 AGCT AG^CT 665 AGCT BanII 99 GAGCCC G[GA]GC[CT]^C 653 GGGCCC BglI 67 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 101 GCCCGGTGGGC BsiEI 65 CGGCCG CG[GA][CT]^CG 203 CGGCCG BspMI 900 ACCTGC ACCTGC(4/8) 1060 GCAGGT BstNI 81 CCAGG CC^[AT]GG 123 CCAGG EaeI 65 CGGCCG [CT]^GGCC[GA] 203 CGGCCG EagI 65 CGGCCG C^GGCCG 203 CGGCCG EclXI 65 CGGCCG C^GGCCG 203 CGGCCG Eco52I 65 CGGCCG C^GGCCG 203 CGGCCG EcoNI 42 CCTCCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 490 CCTGTGGAAGG EcoRII 81 CCAGG ^CC[AT]GG 123 CCAGG HphI 784 GGTGA GGTGA(8/7) 1063 GGTGA MvaI 81 CCAGG CC^[AT]GG 123 CCAGG NotI 64 GCGGCCGC GC^GGCCGC 202 GCGGCCGC PleI 501 GAGTC GAGTC(4/5) 809 GAGTC PpuMI 83 AGGACCC [GA]G^G[AT]CC[CT] 882 GGGACCT SfcI 419 CTGTAG C^T[GA][CT]AG 512 CTGCAG TaqI 324 TCGA T^CGA 846 TCGA Tsp509I 571 AATT ^AATT 1000 AATT TthHB8I 324 TCGA T^CGA 846 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AccII AciI AcyI AfaI AvaII BanI BbeI BbvI BcnI BmyI BsaHI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII DsaI EcoO109I EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI KasI KspI MaeII MaeIII MboII MnlI MspA1I MspI MvnI MwoI NarI NciI NlaIV RsaI SacII Sau96I ScrFI SfoI StyI TseI TspRI