(Circular) (Six-base) MAPSORT of: pFN21AB7626.seq Check: 1319 from: 1 to: 5821 pFN21AB7626 5821 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3056 3056 Size: 5821 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3631 278 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3164 1937 Size: 1227 4594 Acc65I G'GTAC_C Cuts at: 2874 3142 2874 Size: 268 5553 AclI AA'CG_TT Cuts at: 1563 3948 4321 1563 Size: 2385 373 3063 Fragments arranged by size: 3063 2385 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2819 2949 3884 5268 5536 1632 Size: 1187 130 935 1384 268 1917 Fragments arranged by size: 1917 1384 1187 935 268 130 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5624 AflIII A'CryG_T Cuts at: 1203 4720 5697 1203 Size: 3517 977 1327 Fragments arranged by size: 3517 1327 977 AgeI A'CCGG_T Cuts at: 2842 4652 2842 Size: 1810 4011 AhdI GACnn_n'nnGTC Cuts at: 4550 4550 Size: 5821 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5821 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3129 1184 Size: 1945 3876 AlwNI CAG_nnn'CTG Cuts at: 2465 3564 5136 2465 Size: 1099 1572 3150 Fragments arranged by size: 3150 1572 1099 ApaI G_GGCC'C Cuts at: 2662 2662 Size: 5821 ApaLI G'TGCA_C Cuts at: 3878 5034 3878 Size: 1156 4665 ApoI r'AATT_y Cuts at: 1493 3130 3356 3508 5617 1493 Size: 1637 226 152 2109 1697 Fragments arranged by size: 2109 1697 1637 226 152 AseI AT'TA_AT Cuts at: 160 1032 4375 160 Size: 872 3343 1606 Fragments arranged by size: 3343 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 AvaI C'yCGr_G Cuts at: 1870 1943 3146 1870 Size: 73 1203 4545 Fragments arranged by size: 4545 1203 73 BamHI G'GATC_C Cuts at: 3151 3518 3151 Size: 367 5454 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2143 2201 2526 2556 2874 Size: 325 217 627 356 58 325 30 318 Cuts at: 2874 3142 4597 618 Size: 268 1455 1842 Fragments arranged by size: 1842 1455 627 356 325 325 318 268 217 58 30 BanII G_rGCy'C Cuts at: 1391 2108 2662 3140 1391 Size: 717 554 478 4072 Fragments arranged by size: 4072 717 554 478 BbeI G_GCGC'C Cuts at: 2147 2530 2560 2147 Size: 383 30 5408 Fragments arranged by size: 5408 383 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2250 928 Size: 971 351 4499 Fragments arranged by size: 4499 971 351 BbvCI CC'TCA_GC Cuts at: 3117 3117 Size: 5821 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4012 4046 1903 Size: 34 2075 34 3678 Fragments arranged by size: 3678 2075 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3713 4923 3713 Size: 1210 4611 BclI T'GATC_A Cuts at: 1548 1548 Size: 5821 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2078 2112 4432 Size: 107 122 71 631 605 406 34 2320 Cuts at: 4432 136 Size: 1525 Fragments arranged by size: 2320 1525 631 605 406 122 107 71 34 BglII A'GATC_T Cuts at: 5816 5816 Size: 5821 BlpI GC'TnA_GC Cuts at: 3238 3238 Size: 5821 Bme1580I G_kGCm'C Cuts at: 1790 2204 2662 3882 5038 1790 Size: 414 458 1220 1156 2573 Fragments arranged by size: 2573 1220 1156 458 414 BmgBI CAC'GTC Cuts at: 1318 2158 2540 1318 Size: 840 382 4599 Fragments arranged by size: 4599 840 382 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2677 3552 4510 Size: 416 53 83 399 24 1260 875 958 Cuts at: 4510 442 Size: 1753 Fragments arranged by size: 1753 1260 958 875 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5821 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4481 1239 Size: 3242 2579 Bpu10I CC'TnA_GC Cuts at: 3117 3117 Size: 5821 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3946 4811 5109 3946 Size: 865 298 4658 Fragments arranged by size: 4658 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4484 882 Size: 617 2985 2219 Fragments arranged by size: 2985 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2728 3612 493 Size: 711 1524 884 2702 Fragments arranged by size: 2702 1524 884 711 BsaBI GATnn'nnATC Cuts at: 5815 5815 Size: 5821 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2144 2527 2557 2995 Size: 53 83 186 936 611 383 30 438 Cuts at: 2995 3013 3628 4010 4645 275 Size: 18 615 382 635 1451 Fragments arranged by size: 1451 936 635 615 611 438 383 382 186 83 53 30 18 BsaWI w'CCGG_w Cuts at: 2842 4254 4652 4926 5073 5484 2842 Size: 1412 398 274 147 411 3179 Fragments arranged by size: 3179 1412 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2409 2439 2481 2511 1178 Size: 30 165 30 1006 30 42 30 4488 Fragments arranged by size: 4488 1006 165 42 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2603 2606 2609 2883 1186 Size: 1417 3 3 274 4124 Fragments arranged by size: 4124 1417 274 3 3 BseYI C'CCAG_C Cuts at: 2582 2911 3102 5024 5520 2582 Size: 329 191 1922 496 2883 Fragments arranged by size: 2883 1922 496 329 191 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2478 1163 Size: 573 742 4506 Fragments arranged by size: 4506 742 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2073 2211 3271 4032 5060 1540 Size: 275 186 72 138 1060 761 1028 2301 Fragments arranged by size: 2301 1060 1028 761 275 186 138 72 BsiHKAI G_wGCw'C Cuts at: 2830 3882 3967 5038 2830 Size: 1052 85 1071 3613 Fragments arranged by size: 3613 1071 1052 85 BsmI GAATG_Cn' Cuts at: 3332 3425 3332 Size: 93 5728 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 2204 2662 2830 3140 3882 3967 Size: 399 318 96 458 168 310 742 85 Cuts at: 3967 5038 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 458 399 318 310 168 96 85 BspEI T'CCGG_A Cuts at: 5484 5484 Size: 5821 BspHI T'CATG_A Cuts at: 3710 5440 3710 Size: 1730 4091 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2914 3056 3176 844 Size: 2070 142 120 3489 Fragments arranged by size: 3489 2070 142 120 BsrBI CCG'CTC Cuts at: 1394 1823 2607 3708 1394 Size: 429 784 1101 3507 Fragments arranged by size: 3507 1101 784 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4316 4490 65 Size: 1183 3068 174 1396 Fragments arranged by size: 3068 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2234 2700 2842 3009 4465 4652 1700 Size: 110 424 466 142 167 1456 187 2869 Fragments arranged by size: 2869 1456 466 424 187 167 142 110 BsrGI T'GTAC_A Cuts at: 96 2356 96 Size: 2260 3561 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5821 BssSI C'ACGA_G Cuts at: 3881 4893 3881 Size: 1012 4809 BstAPI GCAn_nnn'nTGC Cuts at: 794 2465 794 Size: 1671 4150 BstBI TT'CG_AA Cuts at: 4625 4625 Size: 5821 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5821 BstYI r'GATC_y Cuts at: 1972 3151 3518 3903 3920 5372 5458 5470 5816 Size: 1179 367 385 17 1452 86 12 346 Cuts at: 5816 1972 Size: 1977 Fragments arranged by size: 1977 1452 1179 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2076 2133 2196 2205 2387 2560 2983 Size: 552 1011 57 63 9 182 173 423 Cuts at: 2983 513 Size: 3351 Fragments arranged by size: 3351 1011 552 423 182 173 63 57 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5082 Fragments arranged by size: 5082 683 56 BtsI GCAGTG_nn' Cuts at: 2410 2620 3118 3337 4131 4151 2410 Size: 210 498 219 794 20 4080 Fragments arranged by size: 4080 794 498 219 210 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5821 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5786 DraI TTT'AAA Cuts at: 3125 3125 Size: 5821 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4828 809 Size: 507 3512 1802 Fragments arranged by size: 3512 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2070 2208 3268 Size: 54 1425 50 275 18 240 138 1060 Cuts at: 3268 4157 5786 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1060 889 275 240 138 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2070 2208 3268 1537 Size: 275 258 138 1060 4090 Fragments arranged by size: 4090 1060 275 258 138 EarI CTCTTCn'nnn_ Cuts at: 1353 3751 1353 Size: 2398 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4408 4782 4928 387 Size: 1064 138 2819 374 146 1280 Fragments arranged by size: 2819 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2819 2949 3884 4481 5268 5536 1239 Size: 393 1187 130 935 597 787 268 1524 Fragments arranged by size: 1524 1187 935 787 597 393 268 130 EcoNI CCTnn'n_nnAGG Cuts at: 2051 2499 2051 Size: 448 5373 EcoO109I rG'GnC_Cy Cuts at: 2089 2658 2888 2089 Size: 569 230 5022 Fragments arranged by size: 5022 569 230 EcoRI G'AATT_C Cuts at: 3130 3508 3130 Size: 378 5443 EcoRV GAT'ATC Cuts at: 2990 2990 Size: 5821 FspI TGC'GCA Cuts at: 2402 4327 2402 Size: 1925 3896 HaeII r_GCGC'y Cuts at: 1678 2147 2530 2560 4968 1678 Size: 469 383 30 2408 2531 Fragments arranged by size: 2531 2408 469 383 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2735 2767 4462 4494 4536 Size: 32 135 32 1163 32 1695 32 42 Cuts at: 4536 4568 1373 Size: 32 2626 Fragments arranged by size: 2626 1695 1163 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3165 3411 669 Size: 988 281 1227 246 3079 Fragments arranged by size: 3079 1227 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5821 HpaI GTT'AAC Cuts at: 3411 3411 Size: 5821 KasI G'GCGC_C Cuts at: 2143 2526 2556 2143 Size: 383 30 5408 Fragments arranged by size: 5408 383 30 KpnI G_GTAC'C Cuts at: 2878 3146 2878 Size: 268 5553 MfeI C'AATT_G Cuts at: 3420 3420 Size: 5821 MluI A'CGCG_T Cuts at: 5697 5697 Size: 5821 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2084 2882 2899 4935 5119 1305 Size: 779 798 17 2036 184 2007 Fragments arranged by size: 2036 2007 798 779 184 17 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3999 Fragments arranged by size: 3999 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3779 4138 4297 518 Size: 648 2613 359 159 2042 Fragments arranged by size: 2613 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2078 2118 2135 2207 2671 2707 3237 3914 Size: 472 40 17 72 464 36 530 677 Cuts at: 3914 5062 5307 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 530 472 464 245 72 40 36 17 NaeI GCC'GGC Cuts at: 2236 2236 Size: 5821 NarI GG'CG_CC Cuts at: 2144 2527 2557 2144 Size: 383 30 5408 Fragments arranged by size: 5408 383 30 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5269 NdeI CA'TA_TG Cuts at: 387 387 Size: 5821 NgoMIV G'CCGG_C Cuts at: 2234 2234 Size: 5821 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5821 NotI GC'GGCC_GC Cuts at: 2070 2208 3268 2070 Size: 138 1060 4623 Fragments arranged by size: 4623 1060 138 NspI r_CATG'y Cuts at: 1132 1229 3179 4724 1132 Size: 97 1950 1545 2229 Fragments arranged by size: 2229 1950 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5821 PciI A'CATG_T Cuts at: 4720 4720 Size: 5821 PflMI CCAn_nnn'nTGG Cuts at: 1108 2918 1108 Size: 1810 4011 PmeI GTTT'AAAC Cuts at: 3125 3125 Size: 5821 PmlI CAC'GTG Cuts at: 2728 2728 Size: 5821 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3890 3922 5403 5435 3890 Size: 32 1481 32 4276 Fragments arranged by size: 4276 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2089 2888 2089 Size: 799 5022 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5821 PsiI TTA'TAA Cuts at: 2324 3391 2324 Size: 1067 4754 PspOMI G'GGCC_C Cuts at: 2658 2658 Size: 5821 PstI C_TGCA'G Cuts at: 830 2521 3173 830 Size: 1691 652 3478 Fragments arranged by size: 3478 1691 652 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5821 PvuII CAG'CTG Cuts at: 2671 2671 Size: 5821 SacII CC_GC'GG Cuts at: 2079 2136 2208 2079 Size: 57 72 5692 Fragments arranged by size: 5692 72 57 SalI G'TCGA_C Cuts at: 1936 3163 1936 Size: 1227 4594 SbfI CC_TGCA'GG Cuts at: 3173 3173 Size: 5821 ScaI AGT'ACT Cuts at: 4069 5781 4069 Size: 1712 4109 SfcI C'TryA_G Cuts at: 826 1045 2424 2517 3169 4304 4985 5176 826 Size: 219 1379 93 652 1135 681 191 1471 Fragments arranged by size: 1471 1379 1135 681 652 219 191 93 SfoI GGC'GCC Cuts at: 2145 2528 2558 2145 Size: 383 30 5408 Fragments arranged by size: 5408 383 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5821 SmaI CCC'GGG Cuts at: 1872 3148 1872 Size: 1276 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3925 4826 5088 820 Size: 197 926 1982 901 262 1553 Fragments arranged by size: 1982 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3612 493 Size: 3119 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5821 SphI G_CATG'C Cuts at: 1132 3179 1132 Size: 2047 3774 SspI AAT'ATT Cuts at: 5 52 3745 5 Size: 47 3693 2081 Fragments arranged by size: 3693 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5821 StyI C'CwwG_G Cuts at: 513 1065 2278 2870 3060 3260 513 Size: 552 1213 592 190 200 3074 Fragments arranged by size: 3074 1213 592 552 200 190 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3112 3116 3848 3865 4018 4203 4648 Size: 1255 1532 4 732 17 153 185 445 Cuts at: 4648 325 Size: 1498 Fragments arranged by size: 1532 1498 1255 732 445 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2356 4067 5779 Size: 274 80 33 51 1443 379 1711 1712 Cuts at: 5779 96 Size: 138 Fragments arranged by size: 1712 1711 1443 379 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2481 2513 2944 2976 5781 5813 2481 Size: 32 431 32 2805 32 2489 Fragments arranged by size: 2805 2489 431 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5821 XbaI T'CTAG_A Cuts at: 3157 3157 Size: 5821 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2107 2107 Size: 5821 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5821 XmaI C'CCGG_G Cuts at: 1870 3146 1870 Size: 1276 4545 XmnI GAAnn'nnTTC Cuts at: 3950 3950 Size: 5821 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3629 276 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI