(Circular) (Six-base) MAPSORT of: pFN21AB6390.seq Check: 9827 from: 1 to: 5608 pFN21AB6390 5608 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3418 278 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2951 1937 Size: 1014 4594 Acc65I G'GTAC_C Cuts at: 2929 2929 Size: 5608 AclI AA'CG_TT Cuts at: 1563 3735 4108 1563 Size: 2172 373 3063 Fragments arranged by size: 3063 2172 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2798 3671 5055 5323 1632 Size: 1166 873 1384 268 1917 Fragments arranged by size: 1917 1384 1166 873 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5411 AflIII A'CryG_T Cuts at: 1203 4507 5484 1203 Size: 3304 977 1327 Fragments arranged by size: 3304 1327 977 AgeI A'CCGG_T Cuts at: 4439 4439 Size: 5608 AhdI GACnn_n'nnGTC Cuts at: 4337 4337 Size: 5608 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5608 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5608 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2194 2226 2194 Size: 32 5576 AlwNI CAG_nnn'CTG Cuts at: 3351 4923 3351 Size: 1572 4036 ApaI G_GGCC'C Cuts at: 2044 2104 2291 2044 Size: 60 187 5361 Fragments arranged by size: 5361 187 60 ApaLI G'TGCA_C Cuts at: 3665 4821 3665 Size: 1156 4452 ApoI r'AATT_y Cuts at: 1493 2917 3143 3295 5404 1493 Size: 1424 226 152 2109 1697 Fragments arranged by size: 2109 1697 1424 226 152 AseI AT'TA_AT Cuts at: 160 1032 4162 160 Size: 872 3130 1606 Fragments arranged by size: 3130 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 AvaI C'yCGr_G Cuts at: 1870 1943 2186 2272 2933 1870 Size: 73 243 86 661 4545 Fragments arranged by size: 4545 661 243 86 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2810 2843 2810 Size: 33 5575 BamHI G'GATC_C Cuts at: 2938 3305 2938 Size: 367 5241 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2051 2255 2929 4384 618 Size: 325 217 627 264 204 674 1455 1842 Fragments arranged by size: 1842 1455 674 627 325 264 217 204 BanII G_rGCy'C Cuts at: 1391 2044 2065 2104 2291 2927 1391 Size: 653 21 39 187 636 4072 Fragments arranged by size: 4072 653 636 187 39 21 BbeI G_GCGC'C Cuts at: 2055 2259 2055 Size: 204 5404 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2891 928 Size: 971 992 3645 Fragments arranged by size: 3645 992 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2076 2110 3799 3833 1903 Size: 34 50 34 55 34 1689 34 3678 Fragments arranged by size: 3678 1689 55 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3500 4710 3500 Size: 1210 4398 BclI T'GATC_A Cuts at: 1548 2595 1548 Size: 1047 4561 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2060 2099 2286 Size: 107 122 71 631 605 388 39 187 Cuts at: 2286 4219 136 Size: 1933 1525 Fragments arranged by size: 1933 1525 631 605 388 187 122 107 71 39 BglII A'GATC_T Cuts at: 2566 5603 2566 Size: 3037 2571 BlpI GC'TnA_GC Cuts at: 3025 3025 Size: 5608 Bme1580I G_kGCm'C Cuts at: 1790 2044 2104 2121 2291 3669 4825 1790 Size: 254 60 17 170 1378 1156 2573 Fragments arranged by size: 2573 1378 1156 254 170 60 17 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5608 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2363 2482 3339 Size: 416 53 83 399 24 946 119 857 Cuts at: 3339 4297 442 Size: 958 1753 Fragments arranged by size: 1753 958 946 857 416 399 119 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2012 1056 Size: 956 4652 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2170 2766 4268 1239 Size: 931 596 1502 2579 Fragments arranged by size: 2579 1502 931 596 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3733 4598 4896 3733 Size: 865 298 4445 Fragments arranged by size: 4445 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4271 882 Size: 617 2772 2219 Fragments arranged by size: 2772 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3399 493 Size: 711 2195 2702 Fragments arranged by size: 2702 2195 711 BsaBI GATnn'nnATC Cuts at: 5602 5602 Size: 5608 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2052 2256 3415 3797 Size: 53 83 186 936 519 204 1159 382 Cuts at: 3797 4432 275 Size: 635 1451 Fragments arranged by size: 1451 1159 936 635 519 382 204 186 83 53 BsaWI w'CCGG_w Cuts at: 4041 4439 4713 4860 5271 4041 Size: 398 274 147 411 4378 Fragments arranged by size: 4378 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2143 2173 1178 Size: 30 165 30 740 30 4613 Fragments arranged by size: 4613 740 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5608 BseYI C'CCAG_C Cuts at: 2097 2303 4811 5307 2097 Size: 206 2508 496 2398 Fragments arranged by size: 2508 2398 496 206 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5035 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2078 2166 2247 3058 3819 4847 Size: 275 186 77 88 81 811 761 1028 Cuts at: 4847 1540 Size: 2301 Fragments arranged by size: 2301 1028 811 761 275 186 88 81 77 BsiHKAI G_wGCw'C Cuts at: 2300 2506 3669 3754 4825 2300 Size: 206 1163 85 1071 3083 Fragments arranged by size: 3083 1163 1071 206 85 BsmI GAATG_Cn' Cuts at: 3119 3212 3119 Size: 93 5515 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2044 2065 2104 2121 2291 2300 2506 Size: 399 254 21 39 17 170 9 206 Cuts at: 2506 2927 3669 3754 4825 1391 Size: 421 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 421 399 254 206 170 85 39 21 17 9 BspEI T'CCGG_A Cuts at: 5271 5271 Size: 5608 BspHI T'CATG_A Cuts at: 3497 5227 3497 Size: 1730 3878 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2963 844 Size: 2119 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2168 2252 3495 1394 Size: 429 345 84 1243 3507 Fragments arranged by size: 3507 1243 429 345 84 BsrDI GCAATG_nn' Cuts at: 65 1248 4103 4277 65 Size: 1183 2855 174 1396 Fragments arranged by size: 2855 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4252 4439 1700 Size: 110 2442 187 2869 Fragments arranged by size: 2869 2442 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5608 BssHII G'CGCG_C Cuts at: 1925 2024 1925 Size: 99 5509 BssSI C'ACGA_G Cuts at: 2032 3668 4680 2032 Size: 1636 1012 2960 Fragments arranged by size: 2960 1636 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5608 BstBI TT'CG_AA Cuts at: 4412 4412 Size: 5608 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5608 BstYI r'GATC_y Cuts at: 1972 2566 2938 3305 3690 3707 5159 5245 5257 Size: 594 372 367 385 17 1452 86 12 Cuts at: 5257 5603 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 594 385 372 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2321 513 Size: 552 1256 3800 Fragments arranged by size: 3800 1256 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4869 Fragments arranged by size: 4869 683 56 BtsI GCAGTG_nn' Cuts at: 2700 3124 3918 3938 2700 Size: 424 794 20 4370 Fragments arranged by size: 4370 794 424 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5608 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5573 DraI TTT'AAA Cuts at: 2912 2912 Size: 5608 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4615 809 Size: 507 3299 1802 Fragments arranged by size: 3299 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2075 2139 2163 Size: 54 1425 50 275 18 245 64 24 Cuts at: 2163 2194 2244 2278 2878 3055 3944 5573 8 Size: 31 50 34 600 177 889 1629 43 Fragments arranged by size: 1629 1425 889 600 275 245 177 64 54 50 50 43 34 31 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2075 2163 2244 3055 1537 Size: 275 263 88 81 811 4090 Fragments arranged by size: 4090 811 275 263 88 81 EarI CTCTTCn'nnn_ Cuts at: 1353 2658 3538 1353 Size: 1305 880 3423 Fragments arranged by size: 3423 1305 880 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4195 4569 4715 387 Size: 1064 138 2606 374 146 1280 Fragments arranged by size: 2606 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2170 2766 2798 3671 4268 5055 5323 Size: 393 538 596 32 873 597 787 268 Cuts at: 5323 1239 Size: 1524 Fragments arranged by size: 1524 873 787 597 596 538 393 268 32 EcoO109I rG'GnC_Cy Cuts at: 2040 2041 2101 2287 2040 Size: 1 60 186 5361 Fragments arranged by size: 5361 186 60 1 EcoRI G'AATT_C Cuts at: 2917 3295 2917 Size: 378 5230 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2508 2508 Size: 5608 FspI TGC'GCA Cuts at: 4114 4114 Size: 5608 HaeII r_GCGC'y Cuts at: 1678 2055 2259 4755 1678 Size: 377 204 2496 2531 Fragments arranged by size: 2531 2496 377 204 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2143 2175 4249 4281 4323 Size: 32 135 32 571 32 2074 32 42 Cuts at: 4323 4355 1373 Size: 32 2626 Fragments arranged by size: 2626 2074 571 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2952 3198 669 Size: 988 281 1014 246 3079 Fragments arranged by size: 3079 1014 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5608 HpaI GTT'AAC Cuts at: 3198 3198 Size: 5608 KasI G'GCGC_C Cuts at: 2051 2255 2051 Size: 204 5404 KpnI G_GTAC'C Cuts at: 2933 2933 Size: 5608 MfeI C'AATT_G Cuts at: 3207 3207 Size: 5608 MluI A'CGCG_T Cuts at: 5484 5484 Size: 5608 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2430 2681 4722 4906 1305 Size: 1125 251 2041 184 2007 Fragments arranged by size: 2041 2007 1125 251 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3786 Fragments arranged by size: 3786 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3566 3925 4084 518 Size: 648 2400 359 159 2042 Fragments arranged by size: 2400 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2200 2243 2261 2849 3024 3701 4849 5094 Size: 594 43 18 588 175 677 1148 245 Cuts at: 5094 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 594 588 245 175 43 18 NarI GG'CG_CC Cuts at: 2052 2256 2052 Size: 204 5404 NcoI C'CATG_G Cuts at: 513 1065 2321 513 Size: 552 1256 3800 Fragments arranged by size: 3800 1256 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5608 NheI G'CTAG_C Cuts at: 1052 2008 1052 Size: 956 4652 NotI GC'GGCC_GC Cuts at: 3055 3055 Size: 5608 NspI r_CATG'y Cuts at: 1132 1229 2966 4511 1132 Size: 97 1737 1545 2229 Fragments arranged by size: 2229 1737 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5608 PciI A'CATG_T Cuts at: 4507 4507 Size: 5608 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5608 PfoI T'CCnGG_A Cuts at: 2065 2065 Size: 5608 PmeI GTTT'AAAC Cuts at: 2912 2912 Size: 5608 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3677 3709 5190 5222 3677 Size: 32 1481 32 4063 Fragments arranged by size: 4063 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5608 PsiI TTA'TAA Cuts at: 3178 3178 Size: 5608 PspOMI G'GGCC_C Cuts at: 2040 2100 2287 2040 Size: 60 187 5361 Fragments arranged by size: 5361 187 60 PstI C_TGCA'G Cuts at: 830 2960 830 Size: 2130 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5608 PvuII CAG'CTG Cuts at: 2849 2849 Size: 5608 SalI G'TCGA_C Cuts at: 1936 2950 1936 Size: 1014 4594 SbfI CC_TGCA'GG Cuts at: 2960 2960 Size: 5608 ScaI AGT'ACT Cuts at: 3856 5568 3856 Size: 1712 3896 SfcI C'TryA_G Cuts at: 826 1045 2956 4091 4772 4963 826 Size: 219 1911 1135 681 191 1471 Fragments arranged by size: 1911 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2286 2286 Size: 5608 SfoI GGC'GCC Cuts at: 2053 2257 2053 Size: 204 5404 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5608 SmaI CCC'GGG Cuts at: 1872 2188 2274 2935 1872 Size: 316 86 661 4545 Fragments arranged by size: 4545 661 316 86 SmlI C'TyrA_G Cuts at: 820 1017 1943 3712 4613 4875 820 Size: 197 926 1769 901 262 1553 Fragments arranged by size: 1769 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3399 493 Size: 2906 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5608 SphI G_CATG'C Cuts at: 1132 2966 1132 Size: 1834 3774 SspI AAT'ATT Cuts at: 5 52 2407 3532 5 Size: 47 2355 1125 2081 Fragments arranged by size: 2355 2081 1125 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5608 StyI C'CwwG_G Cuts at: 513 1065 2321 3047 513 Size: 552 1256 726 3074 Fragments arranged by size: 3074 1256 726 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3635 3652 3805 3990 4435 325 Size: 1255 2055 17 153 185 445 1498 Fragments arranged by size: 2055 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3854 5566 96 Size: 274 80 33 51 1443 1877 1712 138 Fragments arranged by size: 1877 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5568 5600 5568 Size: 32 5576 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5608 XbaI T'CTAG_A Cuts at: 2944 2944 Size: 5608 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5608 XmaI C'CCGG_G Cuts at: 1870 2186 2272 2933 1870 Size: 316 86 661 4545 Fragments arranged by size: 4545 661 316 86 XmnI GAAnn'nnTTC Cuts at: 3737 3737 Size: 5608 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3416 276 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SrfI SwaI XcmI