(Circular) (Six-base) MAPSORT of: pFN21AB7801.seq Check: 4253 from: 1 to: 6262 pFN21AB7801 6262 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2607 3143 2607 Size: 536 5726 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2219 4072 278 Size: 53 83 186 936 683 1853 2468 Fragments arranged by size: 2468 1853 936 683 186 83 53 AccI GT'mk_AC Cuts at: 1937 2351 3605 1937 Size: 414 1254 4594 Fragments arranged by size: 4594 1254 414 Acc65I G'GTAC_C Cuts at: 2343 3583 2343 Size: 1240 5022 AclI AA'CG_TT Cuts at: 1563 4389 4762 1563 Size: 2826 373 3063 Fragments arranged by size: 3063 2826 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2665 2906 3038 3056 4325 5709 5977 1632 Size: 1033 241 132 18 1269 1384 268 1917 Fragments arranged by size: 1917 1384 1269 1033 268 241 132 18 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6065 AflIII A'CryG_T Cuts at: 1203 5161 6138 1203 Size: 3958 977 1327 Fragments arranged by size: 3958 1327 977 AgeI A'CCGG_T Cuts at: 5093 5093 Size: 6262 AhdI GACnn_n'nnGTC Cuts at: 4991 4991 Size: 6262 AleI CACnn'nnGTG Cuts at: 1166 2634 1166 Size: 1468 4794 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6262 AlwNI CAG_nnn'CTG Cuts at: 2158 3487 4005 5577 2158 Size: 1329 518 1572 2843 Fragments arranged by size: 2843 1572 1329 518 ApaI G_GGCC'C Cuts at: 2278 2728 3201 2278 Size: 450 473 5339 Fragments arranged by size: 5339 473 450 ApaLI G'TGCA_C Cuts at: 4319 5475 4319 Size: 1156 5106 ApoI r'AATT_y Cuts at: 1493 2892 3571 3797 3949 6058 1493 Size: 1399 679 226 152 2109 1697 Fragments arranged by size: 2109 1697 1399 679 226 152 AseI AT'TA_AT Cuts at: 160 1032 4816 160 Size: 872 3784 1606 Fragments arranged by size: 3784 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 AvaI C'yCGr_G Cuts at: 1870 1943 2075 2271 2721 3331 3587 1870 Size: 73 132 196 450 610 256 4545 Fragments arranged by size: 4545 610 450 256 196 132 73 BamHI G'GATC_C Cuts at: 3592 3959 3592 Size: 367 5895 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2224 2343 3131 3583 5038 Size: 325 217 627 437 119 788 452 1455 Cuts at: 5038 618 Size: 1842 Fragments arranged by size: 1842 1455 788 627 452 437 325 217 119 BanII G_rGCy'C Cuts at: 1391 2076 2278 2728 3201 3255 3338 3581 1391 Size: 685 202 450 473 54 83 243 4072 Fragments arranged by size: 4072 685 473 450 243 202 83 54 BbeI G_GCGC'C Cuts at: 2228 2228 Size: 6262 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5291 BbvCI CC'TCA_GC Cuts at: 2829 2829 Size: 6262 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4453 4487 1903 Size: 34 2516 34 3678 Fragments arranged by size: 3678 2516 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4154 5364 4154 Size: 1210 5052 BclI T'GATC_A Cuts at: 1548 1548 Size: 6262 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4873 136 Size: 107 122 71 631 605 3201 1525 Fragments arranged by size: 3201 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6257 6257 Size: 6262 BlpI GC'TnA_GC Cuts at: 2057 2241 3679 2057 Size: 184 1438 4640 Fragments arranged by size: 4640 1438 184 Bme1580I G_kGCm'C Cuts at: 1790 2278 2728 3201 4323 5479 1790 Size: 488 450 473 1122 1156 2573 Fragments arranged by size: 2573 1156 1122 488 473 450 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6262 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2272 2502 2582 Size: 416 53 83 399 24 855 230 80 Cuts at: 2582 3993 4951 442 Size: 1411 958 1753 Fragments arranged by size: 1753 1411 958 855 416 399 230 83 80 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6262 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2036 2558 2692 2725 2987 3047 3416 3473 Size: 797 522 134 33 262 60 369 57 Cuts at: 3473 3524 4922 1239 Size: 51 1398 2579 Fragments arranged by size: 2579 1398 797 522 369 262 134 60 57 51 33 Bpu10I CC'TnA_GC Cuts at: 2829 2925 3380 2829 Size: 96 455 5711 Fragments arranged by size: 5711 455 96 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4387 5252 5550 4387 Size: 865 298 5099 Fragments arranged by size: 5099 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3437 4925 882 Size: 617 1938 1488 2219 Fragments arranged by size: 2219 1938 1488 617 BsaAI yAC'GTr Cuts at: 493 1204 4053 493 Size: 711 2849 2702 Fragments arranged by size: 2849 2702 711 BsaBI GATnn'nnATC Cuts at: 3414 6256 3414 Size: 2842 3420 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2216 2225 4069 4451 Size: 53 83 186 936 683 9 1844 382 Cuts at: 4451 5086 275 Size: 635 1451 Fragments arranged by size: 1844 1451 936 683 635 382 186 83 53 9 BsaWI w'CCGG_w Cuts at: 4695 5093 5367 5514 5925 4695 Size: 398 274 147 411 5032 Fragments arranged by size: 5032 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2237 2267 2690 2720 3248 Size: 30 165 30 834 30 423 30 528 Cuts at: 3248 3278 1178 Size: 30 4162 Fragments arranged by size: 4162 834 528 423 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2279 2466 2744 2754 2819 2822 2888 3332 Size: 1093 187 278 10 65 3 66 444 Cuts at: 3332 1186 Size: 4116 Fragments arranged by size: 4116 1093 444 278 187 66 65 10 3 BseYI C'CCAG_C Cuts at: 3230 3341 3349 3357 5465 5961 3230 Size: 111 8 8 2108 496 3531 Fragments arranged by size: 3531 2108 496 111 8 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2657 2700 1163 Size: 573 921 43 4725 Fragments arranged by size: 4725 921 573 43 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3712 4473 5501 1540 Size: 275 186 1711 761 1028 2301 Fragments arranged by size: 2301 1711 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2247 4323 4408 5479 2247 Size: 2076 85 1071 3030 Fragments arranged by size: 3030 2076 1071 85 BsmI GAATG_Cn' Cuts at: 3170 3238 3773 3866 3170 Size: 68 535 93 5566 Fragments arranged by size: 5566 535 93 68 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2247 2278 2728 3201 3255 3338 Size: 399 286 171 31 450 473 54 83 Cuts at: 3338 3581 4323 4408 5479 1391 Size: 243 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 473 450 399 286 243 171 85 83 54 31 BspEI T'CCGG_A Cuts at: 5925 5925 Size: 6262 BspHI T'CATG_A Cuts at: 2545 4151 5881 2545 Size: 1606 1730 2926 Fragments arranged by size: 2926 1730 1606 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2607 3113 3143 3294 3617 844 Size: 1763 506 30 151 323 3489 Fragments arranged by size: 3489 1763 506 323 151 30 BsrBI CCG'CTC Cuts at: 1394 1823 2022 4149 1394 Size: 429 199 2127 3507 Fragments arranged by size: 3507 2127 429 199 BsrDI GCAATG_nn' Cuts at: 65 1248 4757 4931 65 Size: 1183 3509 174 1396 Fragments arranged by size: 3509 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4906 5093 1700 Size: 110 3096 187 2869 Fragments arranged by size: 3096 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6262 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6262 BssSI C'ACGA_G Cuts at: 2506 4322 5334 2506 Size: 1816 1012 3434 Fragments arranged by size: 3434 1816 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2035 3244 794 Size: 1241 1209 3812 Fragments arranged by size: 3812 1241 1209 BstBI TT'CG_AA Cuts at: 5066 5066 Size: 6262 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6262 BstXI CCAn_nnnn'nTGG Cuts at: 2458 3220 2458 Size: 762 5500 BstYI r'GATC_y Cuts at: 1972 3275 3415 3592 3959 4344 4361 5813 5899 Size: 1303 140 177 367 385 17 1452 86 Cuts at: 5899 5911 6257 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1303 385 367 346 177 140 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3267 513 Size: 552 939 1263 3508 Fragments arranged by size: 3508 1263 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5523 Fragments arranged by size: 5523 683 56 BtsI GCAGTG_nn' Cuts at: 2048 2641 3778 4572 4592 2048 Size: 593 1137 794 20 3718 Fragments arranged by size: 3718 1137 794 593 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6262 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2181 2216 529 Size: 35 1617 35 4575 Fragments arranged by size: 4575 1617 35 35 DraI TTT'AAA Cuts at: 3566 3566 Size: 6262 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5269 809 Size: 507 3953 1802 Fragments arranged by size: 3953 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3709 4598 6227 Size: 54 1425 50 275 18 1879 889 1629 Cuts at: 6227 8 Size: 43 Fragments arranged by size: 1879 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3709 1537 Size: 275 1897 4090 Fragments arranged by size: 4090 1897 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2177 2807 3538 4192 1353 Size: 824 630 731 654 3423 Fragments arranged by size: 3423 824 731 654 630 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2722 4849 5223 5369 387 Size: 1064 138 1133 2127 374 146 1280 Fragments arranged by size: 2127 1280 1133 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2558 2665 2692 2725 2906 2987 Size: 393 404 522 107 27 33 181 81 Cuts at: 2987 3038 3047 3056 3416 3473 3524 4325 4922 Size: 51 9 9 360 57 51 801 597 Cuts at: 4922 5709 5977 1239 Size: 787 268 1524 Fragments arranged by size: 1524 801 787 597 522 404 393 360 268 181 107 81 57 51 51 33 27 9 9 EcoNI CCTnn'n_nnAGG Cuts at: 2261 2801 2261 Size: 540 5722 EcoO109I rG'GnC_Cy Cuts at: 2089 2725 2786 2817 2881 3198 2089 Size: 636 61 31 64 317 5153 Fragments arranged by size: 5153 636 317 64 61 31 EcoRI G'AATT_C Cuts at: 3571 3949 3571 Size: 378 5884 FspI TGC'GCA Cuts at: 4768 4768 Size: 6262 HaeII r_GCGC'y Cuts at: 1678 2228 3498 5409 1678 Size: 550 1270 1911 2531 Fragments arranged by size: 2531 1911 1270 550 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2908 2940 3308 3340 4903 Size: 32 135 32 1336 32 368 32 1563 Cuts at: 4903 4935 4977 5009 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1563 1336 368 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3606 3852 669 Size: 988 281 1668 246 3079 Fragments arranged by size: 3079 1668 988 281 246 HindIII A'AGCT_T Cuts at: 748 3068 748 Size: 2320 3942 HpaI GTT'AAC Cuts at: 3852 3852 Size: 6262 KasI G'GCGC_C Cuts at: 2224 2224 Size: 6262 KpnI G_GTAC'C Cuts at: 2347 3587 2347 Size: 1240 5022 MfeI C'AATT_G Cuts at: 3861 3861 Size: 6262 MluI A'CGCG_T Cuts at: 6138 6138 Size: 6262 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2439 5376 5560 1305 Size: 1134 2937 184 2007 Fragments arranged by size: 2937 2007 1134 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4440 Fragments arranged by size: 4440 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2634 3146 3218 4220 4579 4738 Size: 648 843 625 512 72 1002 359 159 Cuts at: 4738 518 Size: 2042 Fragments arranged by size: 2042 1002 843 648 625 512 359 159 72 MspA1I CmG'CkG Cuts at: 1606 3678 4355 5503 5748 1606 Size: 2072 677 1148 245 2120 Fragments arranged by size: 2120 2072 1148 677 245 NarI GG'CG_CC Cuts at: 2225 2225 Size: 6262 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4771 Fragments arranged by size: 4771 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6262 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6262 NotI GC'GGCC_GC Cuts at: 3709 3709 Size: 6262 NspI r_CATG'y Cuts at: 1132 1229 3620 5165 1132 Size: 97 2391 1545 2229 Fragments arranged by size: 2391 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6262 PciI A'CATG_T Cuts at: 5161 5161 Size: 6262 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6262 PmeI GTTT'AAAC Cuts at: 3566 3566 Size: 6262 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4331 4363 5844 5876 4331 Size: 32 1481 32 4717 Fragments arranged by size: 4717 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2817 2817 Size: 6262 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6262 PsiI TTA'TAA Cuts at: 3832 3832 Size: 6262 PspOMI G'GGCC_C Cuts at: 2274 2724 3197 2274 Size: 450 473 5339 Fragments arranged by size: 5339 473 450 PstI C_TGCA'G Cuts at: 830 2043 2778 3249 3480 3614 830 Size: 1213 735 471 231 134 3478 Fragments arranged by size: 3478 1213 735 471 231 134 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6262 SalI G'TCGA_C Cuts at: 1936 3604 1936 Size: 1668 4594 SbfI CC_TGCA'GG Cuts at: 3249 3614 3249 Size: 365 5897 ScaI AGT'ACT Cuts at: 4510 6222 4510 Size: 1712 4550 SexAI A'CCwGG_T Cuts at: 3486 3486 Size: 6262 SfcI C'TryA_G Cuts at: 826 1045 2039 2352 2774 3245 3476 3610 4745 Size: 219 994 313 422 471 231 134 1135 Cuts at: 4745 5426 5617 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 994 681 471 422 313 231 219 191 134 SfoI GGC'GCC Cuts at: 2226 2226 Size: 6262 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6262 SmaI CCC'GGG Cuts at: 1872 2077 2723 3333 3589 1872 Size: 205 646 610 256 4545 Fragments arranged by size: 4545 646 610 256 205 SmlI C'TyrA_G Cuts at: 820 1017 1943 4366 5267 5529 820 Size: 197 926 2423 901 262 1553 Fragments arranged by size: 2423 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4053 493 Size: 3560 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6262 SphI G_CATG'C Cuts at: 1132 3620 1132 Size: 2488 3774 SrfI GCCC'GGGC Cuts at: 3333 3333 Size: 6262 SspI AAT'ATT Cuts at: 5 52 4186 5 Size: 47 4134 2081 Fragments arranged by size: 4134 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6262 StyI C'CwwG_G Cuts at: 513 1065 2004 3701 513 Size: 552 939 1697 3074 Fragments arranged by size: 3074 1697 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2306 4289 4306 4459 4644 5089 325 Size: 1255 726 1983 17 153 185 445 1498 Fragments arranged by size: 1983 1498 1255 726 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4508 6220 96 Size: 274 80 33 51 1443 2531 1712 138 Fragments arranged by size: 2531 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2237 2269 2690 2722 6222 6254 2237 Size: 32 421 32 3500 32 2245 Fragments arranged by size: 3500 2245 421 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6262 XbaI T'CTAG_A Cuts at: 3028 3598 3028 Size: 570 5692 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3409 2011 Size: 1398 4864 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6262 XmaI C'CCGG_G Cuts at: 1870 2075 2721 3331 3587 1870 Size: 205 646 610 256 4545 Fragments arranged by size: 4545 646 610 256 205 XmnI GAAnn'nnTTC Cuts at: 4391 4391 Size: 6262 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2217 4070 276 Size: 53 83 186 936 683 1853 2468 Fragments arranged by size: 2468 1853 936 683 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SwaI