(Circular) (Six-base) MAPSORT of: pFN21AE0114.seq Check: 9227 from: 1 to: 5629 pFN21AE0114 5629 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2166 3439 278 Size: 53 83 186 936 630 1273 2468 Fragments arranged by size: 2468 1273 936 630 186 83 53 AccI GT'mk_AC Cuts at: 1937 2052 2972 1937 Size: 115 920 4594 Fragments arranged by size: 4594 920 115 Acc65I G'GTAC_C Cuts at: 2950 2950 Size: 5629 AclI AA'CG_TT Cuts at: 1563 3756 4129 1563 Size: 2193 373 3063 Fragments arranged by size: 3063 2193 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2495 2531 2855 3692 5076 5344 1632 Size: 863 36 324 837 1384 268 1917 Fragments arranged by size: 1917 1384 863 837 324 268 36 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5432 AflIII A'CryG_T Cuts at: 1203 4528 5505 1203 Size: 3325 977 1327 Fragments arranged by size: 3325 1327 977 AgeI A'CCGG_T Cuts at: 4460 4460 Size: 5629 AhdI GACnn_n'nnGTC Cuts at: 4358 4358 Size: 5629 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5629 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5629 AlwNI CAG_nnn'CTG Cuts at: 2470 2527 2791 3372 4944 2470 Size: 57 264 581 1572 3155 Fragments arranged by size: 3155 1572 581 264 57 ApaLI G'TGCA_C Cuts at: 2446 3686 4842 2446 Size: 1240 1156 3233 Fragments arranged by size: 3233 1240 1156 ApoI r'AATT_y Cuts at: 1493 2938 3164 3316 5425 1493 Size: 1445 226 152 2109 1697 Fragments arranged by size: 2109 1697 1445 226 152 AseI AT'TA_AT Cuts at: 160 1032 4183 160 Size: 872 3151 1606 Fragments arranged by size: 3151 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 AvaI C'yCGr_G Cuts at: 1870 1943 2954 1870 Size: 73 1011 4545 Fragments arranged by size: 4545 1011 73 BamHI G'GATC_C Cuts at: 2959 3326 2959 Size: 367 5262 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2950 4405 618 Size: 325 217 627 221 942 1455 1842 Fragments arranged by size: 1842 1455 942 627 325 221 217 BanII G_rGCy'C Cuts at: 1391 2247 2948 1391 Size: 856 701 4072 Fragments arranged by size: 4072 856 701 BbeI G_GCGC'C Cuts at: 2012 2012 Size: 5629 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2158 928 Size: 971 259 4399 Fragments arranged by size: 4399 971 259 BbvCI CC'TCA_GC Cuts at: 2709 2709 Size: 5629 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3820 3854 1903 Size: 34 1883 34 3678 Fragments arranged by size: 3678 1883 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3521 4731 3521 Size: 1210 4419 BclI T'GATC_A Cuts at: 1548 1548 Size: 5629 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4240 136 Size: 107 122 71 631 605 2568 1525 Fragments arranged by size: 2568 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5624 5624 Size: 5629 BlpI GC'TnA_GC Cuts at: 3046 3046 Size: 5629 Bme1580I G_kGCm'C Cuts at: 1790 2450 3690 4846 1790 Size: 660 1240 1156 2573 Fragments arranged by size: 2573 1240 1156 660 BmgBI CAC'GTC Cuts at: 1318 2152 2344 1318 Size: 834 192 4603 Fragments arranged by size: 4603 834 192 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3360 4318 442 Size: 416 53 83 399 24 1943 958 1753 Fragments arranged by size: 1943 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5629 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2231 2462 4289 1239 Size: 992 231 1827 2579 Fragments arranged by size: 2579 1827 992 231 Bpu10I CC'TnA_GC Cuts at: 2241 2709 2241 Size: 468 5161 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3754 4619 4917 3754 Size: 865 298 4466 Fragments arranged by size: 4466 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4292 882 Size: 617 2793 2219 Fragments arranged by size: 2793 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3420 493 Size: 711 2216 2702 Fragments arranged by size: 2702 2216 711 BsaBI GATnn'nnATC Cuts at: 5623 5623 Size: 5629 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2163 3436 3818 Size: 53 83 186 936 476 154 1273 382 Cuts at: 3818 4453 275 Size: 635 1451 Fragments arranged by size: 1451 1273 936 635 476 382 186 154 83 53 BsaWI w'CCGG_w Cuts at: 4062 4460 4734 4881 5292 4062 Size: 398 274 147 411 4399 Fragments arranged by size: 4399 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5404 Fragments arranged by size: 5404 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2221 1186 Size: 1035 4594 BseYI C'CCAG_C Cuts at: 2550 2697 2784 2829 2921 4832 5328 2550 Size: 147 87 45 92 1911 496 2851 Fragments arranged by size: 2851 1911 496 147 92 87 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2408 2483 1163 Size: 573 672 75 4309 Fragments arranged by size: 4309 672 573 75 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3079 3840 4868 1540 Size: 275 186 1078 761 1028 2301 Fragments arranged by size: 2301 1078 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2247 2450 2642 3690 3775 4846 2247 Size: 203 192 1048 85 1071 3030 Fragments arranged by size: 3030 1071 1048 203 192 85 BsmI GAATG_Cn' Cuts at: 2523 3140 3233 2523 Size: 617 93 4919 Fragments arranged by size: 4919 617 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2142 2750 2142 Size: 608 5021 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2247 2450 2642 2948 3690 3775 4846 Size: 399 457 203 192 306 742 85 1071 Cuts at: 4846 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 457 399 306 203 192 85 BspEI T'CCGG_A Cuts at: 5292 5292 Size: 5629 BspHI T'CATG_A Cuts at: 3518 5248 3518 Size: 1730 3899 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2984 844 Size: 2140 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3516 1394 Size: 429 1693 3507 Fragments arranged by size: 3507 1693 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4124 4298 65 Size: 1183 2876 174 1396 Fragments arranged by size: 2876 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4273 4460 1700 Size: 110 2463 187 2869 Fragments arranged by size: 2869 2463 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5629 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5629 BssSI C'ACGA_G Cuts at: 2282 3689 4701 2282 Size: 1407 1012 3210 Fragments arranged by size: 3210 1407 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2470 794 Size: 1676 3953 BstBI TT'CG_AA Cuts at: 4433 4433 Size: 5629 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5629 BstYI r'GATC_y Cuts at: 1972 2466 2959 3326 3711 3728 5180 5266 5278 Size: 494 493 367 385 17 1452 86 12 Cuts at: 5278 5624 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 494 493 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2805 2805 Size: 5629 BtgI C'CryG_G Cuts at: 513 1065 2004 2580 513 Size: 552 939 576 3562 Fragments arranged by size: 3562 939 576 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4890 Fragments arranged by size: 4890 683 56 BtsI GCAGTG_nn' Cuts at: 2108 2559 3145 3939 3959 2108 Size: 451 586 794 20 3778 Fragments arranged by size: 3778 794 586 451 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5629 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2725 2760 529 Size: 35 2161 35 3398 Fragments arranged by size: 3398 2161 35 35 DraI TTT'AAA Cuts at: 2933 2933 Size: 5629 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4636 809 Size: 507 3320 1802 Fragments arranged by size: 3320 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2307 2370 2577 Size: 54 1425 50 275 18 477 63 207 Cuts at: 2577 3076 3965 5594 8 Size: 499 889 1629 43 Fragments arranged by size: 1629 1425 889 499 477 275 207 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3076 1537 Size: 275 1264 4090 Fragments arranged by size: 4090 1264 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2507 3559 1353 Size: 1154 1052 3423 Fragments arranged by size: 3423 1154 1052 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4216 4590 4736 387 Size: 1064 138 2627 374 146 1280 Fragments arranged by size: 2627 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2245 2245 Size: 5629 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 2462 2495 2531 2855 3692 4289 Size: 393 599 231 33 36 324 837 597 Cuts at: 4289 5076 5344 1239 Size: 787 268 1524 Fragments arranged by size: 1524 837 787 599 597 393 324 268 231 36 33 EcoNI CCTnn'n_nnAGG Cuts at: 2531 2720 2531 Size: 189 5440 EcoO109I rG'GnC_Cy Cuts at: 2524 2713 2850 2524 Size: 189 137 5303 Fragments arranged by size: 5303 189 137 EcoRI G'AATT_C Cuts at: 2938 3316 2938 Size: 378 5251 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2095 2095 Size: 5629 FspI TGC'GCA Cuts at: 2258 4135 2258 Size: 1877 3752 FspAI rTGC'GCAy Cuts at: 2258 2258 Size: 5629 HaeII r_GCGC'y Cuts at: 1678 2012 2732 2928 4776 1678 Size: 334 720 196 1848 2531 Fragments arranged by size: 2531 1848 720 334 196 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2624 2656 4270 4302 4344 Size: 32 135 32 1052 32 1614 32 42 Cuts at: 4344 4376 1373 Size: 32 2626 Fragments arranged by size: 2626 1614 1052 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2767 2973 3219 669 Size: 988 281 829 206 246 3079 Fragments arranged by size: 3079 988 829 281 246 206 HindIII A'AGCT_T Cuts at: 748 748 Size: 5629 HpaI GTT'AAC Cuts at: 3219 3219 Size: 5629 KasI G'GCGC_C Cuts at: 2008 2008 Size: 5629 KpnI G_GTAC'C Cuts at: 2954 2954 Size: 5629 MfeI C'AATT_G Cuts at: 3228 3228 Size: 5629 MluI A'CGCG_T Cuts at: 5505 5505 Size: 5629 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2270 4743 4927 1305 Size: 965 2473 184 2007 Fragments arranged by size: 2473 2007 965 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2579 10 Size: 54 1425 343 747 3060 Fragments arranged by size: 3060 1425 747 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2453 2459 3587 3946 4105 518 Size: 648 1287 6 1128 359 159 2042 Fragments arranged by size: 2042 1287 1128 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2122 2697 2708 2833 3045 3722 4870 5115 Size: 516 575 11 125 212 677 1148 245 Cuts at: 5115 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 575 516 245 212 125 11 NarI GG'CG_CC Cuts at: 2009 2009 Size: 5629 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4138 Fragments arranged by size: 4138 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5629 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5629 NotI GC'GGCC_GC Cuts at: 3076 3076 Size: 5629 NspI r_CATG'y Cuts at: 1132 1229 2263 2987 4532 1132 Size: 97 1034 724 1545 2229 Fragments arranged by size: 2229 1545 1034 724 97 PasI CC'CwG_GG Cuts at: 1348 2689 1348 Size: 1341 4288 PciI A'CATG_T Cuts at: 4528 4528 Size: 5629 PflMI CCAn_nnn'nTGG Cuts at: 1108 2565 2626 2791 1108 Size: 1457 61 165 3946 Fragments arranged by size: 3946 1457 165 61 PfoI T'CCnGG_A Cuts at: 2211 2619 2211 Size: 408 5221 PmeI GTTT'AAAC Cuts at: 2933 2933 Size: 5629 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3698 3730 5211 5243 3698 Size: 32 1481 32 4084 Fragments arranged by size: 4084 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2524 2850 2524 Size: 326 5303 PshAI GACnn'nnGTC Cuts at: 1613 2762 1613 Size: 1149 4480 PsiI TTA'TAA Cuts at: 3199 3199 Size: 5629 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2066 2098 2066 Size: 32 5597 PstI C_TGCA'G Cuts at: 830 2601 2724 2981 830 Size: 1771 123 257 3478 Fragments arranged by size: 3478 1771 257 123 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5629 PvuII CAG'CTG Cuts at: 2833 2833 Size: 5629 SacI G_AGCT'C Cuts at: 2247 2247 Size: 5629 SalI G'TCGA_C Cuts at: 1936 2971 1936 Size: 1035 4594 SbfI CC_TGCA'GG Cuts at: 2981 2981 Size: 5629 ScaI AGT'ACT Cuts at: 3877 5589 3877 Size: 1712 3917 SfcI C'TryA_G Cuts at: 826 1045 2597 2720 2909 2977 4112 4793 4984 Size: 219 1552 123 189 68 1135 681 191 Cuts at: 4984 826 Size: 1471 Fragments arranged by size: 1552 1471 1135 681 219 191 189 123 68 SfoI GGC'GCC Cuts at: 2010 2010 Size: 5629 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5629 SmaI CCC'GGG Cuts at: 1872 2956 1872 Size: 1084 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3733 4634 4896 820 Size: 197 926 1790 901 262 1553 Fragments arranged by size: 1790 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3420 493 Size: 2927 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5629 SphI G_CATG'C Cuts at: 1132 2987 1132 Size: 1855 3774 SspI AAT'ATT Cuts at: 5 52 3553 5 Size: 47 3501 2081 Fragments arranged by size: 3501 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5629 StyI C'CwwG_G Cuts at: 513 1065 2004 2880 3068 513 Size: 552 939 876 188 3074 Fragments arranged by size: 3074 939 876 552 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2334 2564 3656 3673 3826 4011 4456 Size: 1255 754 230 1092 17 153 185 445 Cuts at: 4456 325 Size: 1498 Fragments arranged by size: 1498 1255 1092 754 445 230 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3875 5587 96 Size: 274 80 33 51 1443 1898 1712 138 Fragments arranged by size: 1898 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2188 2220 5589 5621 2188 Size: 32 3369 32 2196 Fragments arranged by size: 3369 2196 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5629 XbaI T'CTAG_A Cuts at: 2965 2965 Size: 5629 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5629 XmaI C'CCGG_G Cuts at: 1870 2954 1870 Size: 1084 4545 XmnI GAAnn'nnTTC Cuts at: 2078 3758 2078 Size: 1680 3949 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2164 3437 276 Size: 53 83 186 936 630 1273 2468 Fragments arranged by size: 2468 1273 936 630 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoRV FseI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI