(Circular) (Six-base) MAPSORT of: pFN21AB7835.seq Check: 5608 from: 1 to: 6325 pFN21AB7835 6325 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3591 3591 Size: 6325 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4135 278 Size: 53 83 186 936 2599 2468 Fragments arranged by size: 2599 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2103 3229 3668 1937 Size: 166 1126 439 4594 Fragments arranged by size: 4594 1126 439 166 Acc65I G'GTAC_C Cuts at: 2350 3646 2350 Size: 1296 5029 AclI AA'CG_TT Cuts at: 1563 4452 4825 1563 Size: 2889 373 3063 Fragments arranged by size: 3063 2889 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4388 5772 6040 1632 Size: 2756 1384 268 1917 Fragments arranged by size: 2756 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6128 AflIII A'CryG_T Cuts at: 1203 2127 5224 6201 1203 Size: 924 3097 977 1327 Fragments arranged by size: 3097 1327 977 924 AgeI A'CCGG_T Cuts at: 5156 5156 Size: 6325 AhdI GACnn_n'nnGTC Cuts at: 5054 5054 Size: 6325 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6325 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2259 1184 Size: 1075 5250 AlwNI CAG_nnn'CTG Cuts at: 4068 5640 4068 Size: 1572 4753 ApaLI G'TGCA_C Cuts at: 2244 4382 5538 2244 Size: 2138 1156 3031 Fragments arranged by size: 3031 2138 1156 ApoI r'AATT_y Cuts at: 1493 2454 2951 3197 3634 3860 4012 6121 1493 Size: 961 497 246 437 226 152 2109 1697 Fragments arranged by size: 2109 1697 961 497 437 246 226 152 AseI AT'TA_AT Cuts at: 160 1032 2747 4879 160 Size: 872 1715 2132 1606 Fragments arranged by size: 2132 1715 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6325 AvaI C'yCGr_G Cuts at: 1870 1943 3650 1870 Size: 73 1707 4545 Fragments arranged by size: 4545 1707 73 AvrII C'CTAG_G Cuts at: 2411 2411 Size: 6325 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2334 2367 2334 Size: 33 6292 BamHI G'GATC_C Cuts at: 3006 3655 4022 3006 Size: 649 367 5309 Fragments arranged by size: 5309 649 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2350 3448 3646 5101 618 Size: 325 217 627 563 1098 198 1455 1842 Fragments arranged by size: 1842 1455 1098 627 563 325 217 198 BanII G_rGCy'C Cuts at: 1391 2773 3644 1391 Size: 1382 871 4072 Fragments arranged by size: 4072 1382 871 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2136 3333 928 Size: 971 237 1197 3920 Fragments arranged by size: 3920 1197 971 237 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2531 2565 4516 4550 1903 Size: 34 594 34 1951 34 3678 Fragments arranged by size: 3678 1951 594 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4217 5427 4217 Size: 1210 5115 BclI T'GATC_A Cuts at: 1548 1548 Size: 6325 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4936 136 Size: 107 122 71 631 605 3264 1525 Fragments arranged by size: 3264 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6320 6320 Size: 6325 BlpI GC'TnA_GC Cuts at: 3612 3742 3612 Size: 130 6195 Bme1580I G_kGCm'C Cuts at: 1790 2248 3451 4386 5542 1790 Size: 458 1203 935 1156 2573 Fragments arranged by size: 2573 1203 1156 935 458 BmgBI CAC'GTC Cuts at: 1318 2332 1318 Size: 1014 5311 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4056 5014 442 Size: 416 53 83 399 24 2639 958 1753 Fragments arranged by size: 2639 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6325 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2845 2979 3544 4985 1239 Size: 1606 134 565 1441 2579 Fragments arranged by size: 2579 1606 1441 565 134 Bpu10I CC'TnA_GC Cuts at: 3537 3537 Size: 6325 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3030 3219 4450 5315 5613 3030 Size: 189 1231 865 298 3742 Fragments arranged by size: 3742 1231 865 298 189 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4988 882 Size: 617 3489 2219 Fragments arranged by size: 3489 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4116 493 Size: 711 2912 2702 Fragments arranged by size: 2912 2702 711 BsaBI GATnn'nnATC Cuts at: 6319 6319 Size: 6325 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4132 4514 5149 275 Size: 53 83 186 936 2599 382 635 1451 Fragments arranged by size: 2599 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4758 5156 5430 5577 5988 4758 Size: 398 274 147 411 5095 Fragments arranged by size: 5095 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3351 3381 1178 Size: 30 165 30 1948 30 4122 Fragments arranged by size: 4122 1948 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3375 1186 Size: 2189 4136 BseYI C'CCAG_C Cuts at: 2695 5528 6024 2695 Size: 2833 496 2996 Fragments arranged by size: 2996 2833 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5752 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3775 4536 5564 1540 Size: 275 186 1774 761 1028 2301 Fragments arranged by size: 2301 1774 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2248 2773 4386 4471 5542 2248 Size: 525 1613 85 1071 3031 Fragments arranged by size: 3031 1613 1071 525 85 BsmI GAATG_Cn' Cuts at: 3836 3929 3836 Size: 93 6232 BsmBI CGTCTCn'nnnn_ Cuts at: 3357 3357 Size: 6325 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2248 2773 3451 3644 4386 4471 5542 Size: 399 458 525 678 193 742 85 1071 Cuts at: 5542 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 678 525 458 399 193 85 BspEI T'CCGG_A Cuts at: 5988 5988 Size: 6325 BspHI T'CATG_A Cuts at: 4214 5944 4214 Size: 1730 4595 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3591 3680 844 Size: 2747 89 3489 Fragments arranged by size: 3489 2747 89 BsrBI CCG'CTC Cuts at: 1394 1823 4212 1394 Size: 429 2389 3507 Fragments arranged by size: 3507 2389 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4820 4994 65 Size: 1183 3572 174 1396 Fragments arranged by size: 3572 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4969 5156 1700 Size: 110 3159 187 2869 Fragments arranged by size: 3159 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6325 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6325 BssSI C'ACGA_G Cuts at: 4385 5397 4385 Size: 1012 5313 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6325 BstBI TT'CG_AA Cuts at: 5129 5129 Size: 6325 BstEII G'GTnAC_C Cuts at: 1184 2732 1184 Size: 1548 4777 BstYI r'GATC_y Cuts at: 1972 2872 3006 3655 4022 4407 4424 5876 5962 Size: 900 134 649 367 385 17 1452 86 Cuts at: 5962 5974 6320 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 900 649 385 367 346 134 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4834 Fragments arranged by size: 4834 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5586 Fragments arranged by size: 5586 683 56 BtsI GCAGTG_nn' Cuts at: 3393 3841 4635 4655 3393 Size: 448 794 20 5063 Fragments arranged by size: 5063 794 448 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6325 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6290 DraI TTT'AAA Cuts at: 3629 3629 Size: 6325 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5332 809 Size: 507 4016 1802 Fragments arranged by size: 4016 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3313 3772 4661 Size: 54 1425 50 275 18 1483 459 889 Cuts at: 4661 6290 8 Size: 1629 43 Fragments arranged by size: 1629 1483 1425 889 459 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3772 1537 Size: 275 1960 4090 Fragments arranged by size: 4090 1960 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2761 3341 4255 1353 Size: 1408 580 914 3423 Fragments arranged by size: 3423 1408 914 580 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4912 5286 5432 387 Size: 1064 138 3323 374 146 1280 Fragments arranged by size: 3323 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2771 2771 Size: 6325 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2845 2979 3544 4388 4985 5772 6040 Size: 393 1213 134 565 844 597 787 268 Cuts at: 6040 1239 Size: 1524 Fragments arranged by size: 1524 1213 844 787 597 565 393 268 134 EcoNI CCTnn'n_nnAGG Cuts at: 3487 3487 Size: 6325 EcoRI G'AATT_C Cuts at: 2454 3634 4012 2454 Size: 1180 378 4767 Fragments arranged by size: 4767 1180 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2790 2790 Size: 6325 FspI TGC'GCA Cuts at: 4831 4831 Size: 6325 HaeII r_GCGC'y Cuts at: 1678 5472 1678 Size: 3794 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2864 2885 2896 2917 4966 Size: 32 135 32 1292 21 11 21 2049 Cuts at: 4966 4998 5040 5072 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2049 1292 135 42 32 32 32 32 21 21 11 HincII GTy'rAC Cuts at: 669 1657 1938 2104 3087 3230 3669 3915 669 Size: 988 281 166 983 143 439 246 3079 Fragments arranged by size: 3079 988 983 439 281 246 166 143 HindIII A'AGCT_T Cuts at: 748 748 Size: 6325 HpaI GTT'AAC Cuts at: 3087 3915 3087 Size: 828 5497 KpnI G_GTAC'C Cuts at: 2354 3650 2354 Size: 1296 5029 MfeI C'AATT_G Cuts at: 3924 3924 Size: 6325 MluI A'CGCG_T Cuts at: 6201 6201 Size: 6325 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2761 2900 5439 5623 1305 Size: 1456 139 2539 184 2007 Fragments arranged by size: 2539 2007 1456 184 139 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4503 Fragments arranged by size: 4503 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4283 4642 4801 518 Size: 648 3117 359 159 2042 Fragments arranged by size: 3117 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2206 3741 4418 5566 5811 1606 Size: 600 1535 677 1148 245 2120 Fragments arranged by size: 2120 1535 1148 677 600 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4834 Fragments arranged by size: 4834 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6325 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6325 NotI GC'GGCC_GC Cuts at: 3772 3772 Size: 6325 NspI r_CATG'y Cuts at: 1132 1229 2068 2131 3683 5228 1132 Size: 97 839 63 1552 1545 2229 Fragments arranged by size: 2229 1552 1545 839 97 63 PasI CC'CwG_GG Cuts at: 1348 3210 1348 Size: 1862 4463 PciI A'CATG_T Cuts at: 2127 5224 2127 Size: 3097 3228 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6325 PmeI GTTT'AAAC Cuts at: 3629 3629 Size: 6325 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4394 4426 5907 5939 4394 Size: 32 1481 32 4780 Fragments arranged by size: 4780 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6325 PsiI TTA'TAA Cuts at: 3895 3895 Size: 6325 PstI C_TGCA'G Cuts at: 830 3388 3677 830 Size: 2558 289 3478 Fragments arranged by size: 3478 2558 289 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6325 PvuII CAG'CTG Cuts at: 2206 2206 Size: 6325 SacI G_AGCT'C Cuts at: 2773 2773 Size: 6325 SalI G'TCGA_C Cuts at: 1936 2102 3228 3667 1936 Size: 166 1126 439 4594 Fragments arranged by size: 4594 1126 439 166 SapI GCTCTTCn'nnn_ Cuts at: 2761 2761 Size: 6325 SbfI CC_TGCA'GG Cuts at: 3677 3677 Size: 6325 ScaI AGT'ACT Cuts at: 3266 4573 6285 3266 Size: 1307 1712 3306 Fragments arranged by size: 3306 1712 1307 SexAI A'CCwGG_T Cuts at: 2049 2049 Size: 6325 SfcI C'TryA_G Cuts at: 826 1045 2134 3384 3673 4808 5489 5680 826 Size: 219 1089 1250 289 1135 681 191 1471 Fragments arranged by size: 1471 1250 1135 1089 681 289 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6325 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6325 SmaI CCC'GGG Cuts at: 1872 3652 1872 Size: 1780 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3045 3234 4429 5330 5592 820 Size: 197 926 1102 189 1195 901 262 1553 Fragments arranged by size: 1553 1195 1102 926 901 262 197 189 SnaBI TAC'GTA Cuts at: 493 4116 493 Size: 3623 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6325 SphI G_CATG'C Cuts at: 1132 3683 1132 Size: 2551 3774 SspI AAT'ATT Cuts at: 5 52 4249 5 Size: 47 4197 2081 Fragments arranged by size: 4197 2081 47 StuI AGG'CCT Cuts at: 1517 2253 3162 1517 Size: 736 909 4680 Fragments arranged by size: 4680 909 736 StyI C'CwwG_G Cuts at: 513 1065 2004 2411 2757 3764 513 Size: 552 939 407 346 1007 3074 Fragments arranged by size: 3074 1007 939 552 407 346 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2151 2724 4352 4369 4522 4707 5152 Size: 1255 571 573 1628 17 153 185 445 Cuts at: 5152 325 Size: 1498 Fragments arranged by size: 1628 1498 1255 573 571 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2130 2936 3264 Size: 274 80 33 51 1443 153 806 328 Cuts at: 3264 4571 6283 96 Size: 1307 1712 138 Fragments arranged by size: 1712 1443 1307 806 328 274 153 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3572 3604 6285 6317 3572 Size: 32 2681 32 3580 Fragments arranged by size: 3580 2681 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6325 XbaI T'CTAG_A Cuts at: 3661 3661 Size: 6325 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2435 2435 Size: 6325 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6325 XmaI C'CCGG_G Cuts at: 1870 3650 1870 Size: 1780 4545 XmnI GAAnn'nnTTC Cuts at: 2841 3330 4454 2841 Size: 489 1124 4712 Fragments arranged by size: 4712 1124 489 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4133 276 Size: 53 83 186 936 2599 2468 Fragments arranged by size: 2599 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI