Restriction map for
pFN21AE0494
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwNI AocI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbsI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsiWI BsmAI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrI Bst1107I
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraII DraIII DrdI
Eam1105I Eco81I EcoNI EcoO109I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI MaeI MamI MfeI MluI
MluNI MroI MscI MseI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SalI SbfI SexAI
SfaNI SfcI SfiI SfuI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI TfiI Tru9I
Tsp509I Tth111I TthHB8I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AcyI 550 GGCGCC G[GA]^CG[CT]C
Alw44I 309 GTGCAC G^TGCAC
Aor51HI 486 AGCGCT AGC^GCT
ApaI 330 GGGCCC GGGCC^C
ApaLI 309 GTGCAC G^TGCAC
AvaI 327 CCCGGG C^[CT]CG[GA]G
BanI 550 GGCGCC G^G[CT][GA]CC
BanII 330 GGGCCC G[GA]GC[CT]^C
BbeI 550 GGCGCC GGCGC^C
BbvCI 579 CCTCAGC CCTCAGC(-5/-2)
BsaHI 550 GGCGCC G[GA]^CG[CT]C
BsiEI 567 CGGCCG CG[GA][CT]^CG
BsmFI 572 GTCCC GGGAC(10/14)
BsoBI 327 CCCGGG C^[CT]CG[GA]G
Bsp1407I 491 TGTACA T^GTACA
BsrGI 491 TGTACA T^GTACA
BssHII 302 GCGCGC G^CGCGC
BssSI 397 CTCGTG CACGAG(-5/-1)
BstAPI 311 GCACCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 93 GGTGACC G^GT[ACGT]ACC
BstF5I 152 GGATG GGATG(2/0)
BstPI 93 GGTGACC G^GT[ACGT]ACC
BstYI 20 AGATCC [GA]^GATC[CT]
EagI 567 CGGCCG C^GGCCG
EarI 283 CTCTTC CTCTTC(1/4)
EclXI 567 CGGCCG C^GGCCG
Eco47III 486 AGCGCT AGC^GCT
Eco52I 567 CGGCCG C^GGCCG
EcoO65I 93 GGTGACC G^GT[ACGT]ACC
EcoT14I 338 CCAAGG C^C[AT][AT]GG
FokI 152 GGATG GGATG(9/13)
Hin1I 550 GGCGCC G[GA]^CG[CT]C
HinfI 202 GACTC G^A[ACGT]TC
KasI 550 GGCGCC G^GCGCC
Ksp632I 283 CTCTTC CTCTTC(1/4)
KspI 607 CCGCGG CCGC^GG
MaeII 650 ACGT A^CGT
MflI 20 AGATCC [GA]^GATC[CT]
MspA1I 607 CCGCGG C[AC]G^C[GT]G
NarI 550 GGCGCC GG^CGCC
NspI 182 ACATGC [GA]CATG^[CT]
PleI 202 GACTC GAGTC(4/5)
SacII 607 CCGCGG CCGC^GG
SapI 282 GCTCTTC GCTCTTC(1/4)
ScaI 596 AGTACT AGT^ACT
SfoI 550 GGCGCC GGC^GCC
SgrAI 624 CACCGGCG C[GA]^CCGG[CT]G
SmaI 327 CCCGGG CCC^GGG
SspBI 491 TGTACA T^GTACA
StyI 338 CCAAGG C^C[AT][AT]GG
XcmI 338 CCAAGGCGCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 20 AGATCC [GA]^GATC[CT]
XmaCI 327 CCCGGG C^CCGGG
XmaI 327 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AluI 476 AGCT AG^CT
583 AGCT
AlwI 21 GATCC GGATC(4/5)
63 GATCC
AvaII 5 GGTCC G^G[AT]CC
108 GGACC
BspMI 316 ACCTGC ACCTGC(4/8)
90 GCAGGT
BsrFI 565 ACCGGC [GA]^CCGG[CT]
625 ACCGGC
BstXI 314 CCACCTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
403 CCACTCTGCTGG
Cfr10I 565 ACCGGC [GA]^CCGG[CT]
625 ACCGGC
DpnII 21 GATC ^GATC
63 GATC
DsaI 607 CCGCGG C^C[GA][CT]GG
617 CCACGG
HphI 93 GGTGA GGTGA(8/7)
248 TCACC
MboI 21 GATC ^GATC
63 GATC
MboII 642 GAAGA GAAGA(8/7)
284 TCTTC
MslI 222 CACCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
315 CACCTGCGTG
NdeII 21 GATC ^GATC
63 GATC
Sau3AI 21 GATC ^GATC
63 GATC
TspRI 187 CAGTG CA[GC]TG[ACGT][ACGT]^
307 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AfaI AspHI BbvI
BcnI BglI BmyI BsaJI BsgI
BsiHKAI BsiYI BslI Bsp1286I BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI EaeI EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HpaII ItaI MaeIII MnlI MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV RsaI Sau96I ScrFI TseI
Tsp45I