Restriction map for
pFN21AE0494
(Linear) (Six-base) MAPSORT of: KIEE0494.seq Check: 7195 from: 1 to: 654
KIEE0494.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 81 325 654
Size: 81 244 329
Fragments arranged by size:
329 244 81
AfeI AGC'GCT
Cuts at: 0 488 654
Size: 488 166
AleI CACnn'nnGTG
Cuts at: 0 226 319 654
Size: 226 93 335
Fragments arranged by size:
335 226 93
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 244 654
Size: 244 410
ApaI G_GGCC'C
Cuts at: 0 334 654
Size: 334 320
ApaLI G'TGCA_C
Cuts at: 0 309 654
Size: 309 345
AvaI C'yCGr_G
Cuts at: 0 327 654
Size: 327 327
BanI G'GyrC_C
Cuts at: 0 550 654
Size: 550 104
BanII G_rGCy'C
Cuts at: 0 334 654
Size: 334 320
BbeI G_GCGC'C
Cuts at: 0 554 654
Size: 554 100
BbvCI CC'TCA_GC
Cuts at: 0 580 654
Size: 580 74
BglI GCCn_nnn'nGGC
Cuts at: 0 47 87 612 654
Size: 47 40 525 42
Fragments arranged by size:
525 47 42 40
Bme1580I G_kGCm'C
Cuts at: 0 182 313 334 458 654
Size: 182 131 21 124 196
Fragments arranged by size:
196 182 131 124 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 385 654
Size: 385 269
Bpu10I CC'TnA_GC
Cuts at: 0 580 654
Size: 580 74
BsaHI Gr'CG_yC
Cuts at: 0 551 654
Size: 551 103
BseYI C'CCAG_C
Cuts at: 0 50 477 500 654
Size: 50 427 23 154
Fragments arranged by size:
427 154 50 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 250 546 654
Size: 187 63 296 108
Fragments arranged by size:
296 187 108 63
BsiEI CG_ry'CG
Cuts at: 0 570 654
Size: 570 84
BsiHKAI G_wGCw'C
Cuts at: 0 175 223 313 371 654
Size: 175 48 90 58 283
Fragments arranged by size:
283 175 90 58 48
Bsp1286I G_dGCh'C
Cuts at: 0 175 182 223 313 334 371 458 654
Size: 175 7 41 90 21 37 87 196
Fragments arranged by size:
196 175 90 87 41 37 21 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 81 325 654
Size: 81 244 329
Fragments arranged by size:
329 244 81
BsrFI r'CCGG_y
Cuts at: 0 565 625 654
Size: 565 60 29
Fragments arranged by size:
565 60 29
BsrGI T'GTAC_A
Cuts at: 0 491 654
Size: 491 163
BssHII G'CGCG_C
Cuts at: 0 302 654
Size: 302 352
BssSI C'ACGA_G
Cuts at: 0 397 654
Size: 397 257
BstAPI GCAn_nnn'nTGC
Cuts at: 0 317 654
Size: 317 337
BstEII G'GTnAC_C
Cuts at: 0 93 654
Size: 93 561
BstXI CCAn_nnnn'nTGG
Cuts at: 0 321 410 654
Size: 321 89 244
Fragments arranged by size:
321 244 89
BstYI r'GATC_y
Cuts at: 0 20 654
Size: 20 634
BtgI C'CryG_G
Cuts at: 0 607 617 654
Size: 607 10 37
Fragments arranged by size:
607 37 10
BtsI GCAGTG_nn'
Cuts at: 0 193 313 654
Size: 193 120 341
Fragments arranged by size:
341 193 120
EaeI y'GGCC_r
Cuts at: 0 335 506 567 620 654
Size: 335 171 61 53 34
Fragments arranged by size:
335 171 61 53 34
EagI C'GGCC_G
Cuts at: 0 567 654
Size: 567 87
EarI CTCTTCn'nnn_
Cuts at: 0 289 654
Size: 289 365
HaeII r_GCGC'y
Cuts at: 0 247 260 397 490 554 607 654
Size: 247 13 137 93 64 53 47
Fragments arranged by size:
247 137 93 64 53 47 13
KasI G'GCGC_C
Cuts at: 0 550 654
Size: 550 104
MslI CAynn'nnrTG
Cuts at: 0 226 319 654
Size: 226 93 335
Fragments arranged by size:
335 226 93
MspA1I CmG'CkG
Cuts at: 0 609 654
Size: 609 45
NarI GG'CG_CC
Cuts at: 0 551 654
Size: 551 103
NspI r_CATG'y
Cuts at: 0 186 654
Size: 186 468
PfoI T'CCnGG_A
Cuts at: 0 104 654
Size: 104 550
PspOMI G'GGCC_C
Cuts at: 0 330 654
Size: 330 324
SacII CC_GC'GG
Cuts at: 0 610 654
Size: 610 44
SapI GCTCTTCn'nnn_
Cuts at: 0 289 654
Size: 289 365
ScaI AGT'ACT
Cuts at: 0 598 654
Size: 598 56
SfoI GGC'GCC
Cuts at: 0 552 654
Size: 552 102
SgrAI Cr'CCGG_yG
Cuts at: 0 625 654
Size: 625 29
SmaI CCC'GGG
Cuts at: 0 329 654
Size: 329 325
StyI C'CwwG_G
Cuts at: 0 338 654
Size: 338 316
TatI w'GTAC_w
Cuts at: 0 491 596 654
Size: 491 105 58
Fragments arranged by size:
491 105 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 555 587 654
Size: 555 32 67
Fragments arranged by size:
555 67 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 345 654
Size: 345 309
XmaI C'CCGG_G
Cuts at: 0 327 654
Size: 327 327
Enzymes that do cut:
AarI AfeI AleI AlfI ApaI ApaLI AvaI BanI
BanII BbeI BbvCI BglI Bme1580I BplI Bpu10I BsaHI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtsI
EaeI EagI EarI HaeII KasI MslI MspA1I NarI
NspI PfoI PspOMI SacII SapI ScaI SfoI SgrAI
SmaI StyI TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AloI AlwNI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BstBI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SbfI SexAI SfcI SfiI SgfI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XhoI XmnI ZraI