(Circular) (Six-base) MAPSORT of: pFN21AB7179.seq Check: 9605 from: 1 to: 6142 pFN21AB7179 6142 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3952 278 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3485 1937 Size: 1548 4594 Acc65I G'GTAC_C Cuts at: 3463 3463 Size: 6142 AclI AA'CG_TT Cuts at: 1563 4269 4642 1563 Size: 2706 373 3063 Fragments arranged by size: 3063 2706 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2370 2723 4205 5589 5857 1632 Size: 738 353 1482 1384 268 1917 Fragments arranged by size: 1917 1482 1384 738 353 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5945 AflIII A'CryG_T Cuts at: 1203 3036 5041 6018 1203 Size: 1833 2005 977 1327 Fragments arranged by size: 2005 1833 1327 977 AgeI A'CCGG_T Cuts at: 4973 4973 Size: 6142 AhdI GACnn_n'nnGTC Cuts at: 4871 4871 Size: 6142 AleI CACnn'nnGTG Cuts at: 1166 2405 2979 1166 Size: 1239 574 4329 Fragments arranged by size: 4329 1239 574 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6142 AlwNI CAG_nnn'CTG Cuts at: 2393 3885 5457 2393 Size: 1492 1572 3078 Fragments arranged by size: 3078 1572 1492 ApaLI G'TGCA_C Cuts at: 3258 4199 5355 3258 Size: 941 1156 4045 Fragments arranged by size: 4045 1156 941 ApoI r'AATT_y Cuts at: 1493 2229 3451 3677 3829 5938 1493 Size: 736 1222 226 152 2109 1697 Fragments arranged by size: 2109 1697 1222 736 226 152 AseI AT'TA_AT Cuts at: 160 1032 4696 160 Size: 872 3664 1606 Fragments arranged by size: 3664 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 AvaI C'yCGr_G Cuts at: 1870 1943 3467 1870 Size: 73 1524 4545 Fragments arranged by size: 4545 1524 73 BamHI G'GATC_C Cuts at: 2250 2322 3472 3839 2250 Size: 72 1150 367 4553 Fragments arranged by size: 4553 1150 367 72 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3001 3463 4918 618 Size: 325 217 627 1214 462 1455 1842 Fragments arranged by size: 1842 1455 1214 627 462 325 217 BanII G_rGCy'C Cuts at: 1391 3461 1391 Size: 2070 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5171 BbvCI CC'TCA_GC Cuts at: 3080 3080 Size: 6142 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4333 4367 1903 Size: 34 2396 34 3678 Fragments arranged by size: 3678 2396 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4034 5244 4034 Size: 1210 4932 BclI T'GATC_A Cuts at: 1548 2430 2931 3423 1548 Size: 882 501 492 4267 Fragments arranged by size: 4267 882 501 492 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2924 4753 136 Size: 107 122 71 631 605 1252 1829 1525 Fragments arranged by size: 1829 1525 1252 631 605 122 107 71 BglII A'GATC_T Cuts at: 2581 6137 2581 Size: 3556 2586 BlpI GC'TnA_GC Cuts at: 3559 3559 Size: 6142 Bme1580I G_kGCm'C Cuts at: 1790 2895 3262 4203 5359 1790 Size: 1105 367 941 1156 2573 Fragments arranged by size: 2573 1156 1105 941 367 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6142 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2322 3019 3873 Size: 416 53 83 399 24 905 697 854 Cuts at: 3873 4831 442 Size: 958 1753 Fragments arranged by size: 1753 958 905 854 697 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6142 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4802 1239 Size: 3563 2579 Bpu10I CC'TnA_GC Cuts at: 3080 3080 Size: 6142 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4267 5132 5430 4267 Size: 865 298 4979 Fragments arranged by size: 4979 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2815 4805 882 Size: 617 1316 1990 2219 Fragments arranged by size: 2219 1990 1316 617 BsaAI yAC'GTr Cuts at: 493 1204 2283 3933 493 Size: 711 1079 1650 2702 Fragments arranged by size: 2702 1650 1079 711 BsaBI GATnn'nnATC Cuts at: 2274 2969 6136 2274 Size: 695 3167 2280 Fragments arranged by size: 3167 2280 695 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3949 4331 4966 275 Size: 53 83 186 936 2416 382 635 1451 Fragments arranged by size: 2416 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2798 4575 4973 5247 5394 5805 2798 Size: 1777 398 274 147 411 3135 Fragments arranged by size: 3135 1777 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2793 2823 1178 Size: 30 165 30 1390 30 4497 Fragments arranged by size: 4497 1390 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2722 2726 2760 1186 Size: 1536 4 34 4568 Fragments arranged by size: 4568 1536 34 4 BseYI C'CCAG_C Cuts at: 2788 5345 5841 2788 Size: 2557 496 3089 Fragments arranged by size: 3089 2557 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5569 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2999 3592 4353 5381 1540 Size: 275 186 998 593 761 1028 2301 Fragments arranged by size: 2301 1028 998 761 593 275 186 BsiHKAI G_wGCw'C Cuts at: 3262 4203 4288 5359 3262 Size: 941 85 1071 4045 Fragments arranged by size: 4045 1071 941 85 BsmI GAATG_Cn' Cuts at: 3653 3746 3653 Size: 93 6049 BsmBI CGTCTCn'nnnn_ Cuts at: 2069 2069 Size: 6142 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2895 3262 3461 4203 4288 5359 1391 Size: 399 1105 367 199 742 85 1071 2174 Fragments arranged by size: 2174 1105 1071 742 399 367 199 85 BspEI T'CCGG_A Cuts at: 2798 5805 2798 Size: 3007 3135 BspHI T'CATG_A Cuts at: 4031 5761 4031 Size: 1730 4412 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3497 844 Size: 2653 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3103 3437 4029 1394 Size: 429 1280 334 592 3507 Fragments arranged by size: 3507 1280 592 429 334 BsrDI GCAATG_nn' Cuts at: 65 1248 4637 4811 65 Size: 1183 3389 174 1396 Fragments arranged by size: 3389 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2446 2875 2998 4786 4973 1700 Size: 110 636 429 123 1788 187 2869 Fragments arranged by size: 2869 1788 636 429 187 123 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6142 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6142 BssSI C'ACGA_G Cuts at: 2336 4202 5214 2336 Size: 1866 1012 3264 Fragments arranged by size: 3264 1866 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6142 BstBI TT'CG_AA Cuts at: 4946 4946 Size: 6142 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6142 BstXI CCAn_nnnn'nTGG Cuts at: 2833 2981 2833 Size: 148 5994 BstYI r'GATC_y Cuts at: 1972 2040 2250 2322 2581 3472 3839 4224 4241 Size: 68 210 72 259 891 367 385 17 Cuts at: 4241 5693 5779 5791 6137 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 891 385 367 346 259 210 86 72 68 17 12 Bsu36I CC'TnA_GG Cuts at: 3237 3237 Size: 6142 BtgI C'CryG_G Cuts at: 513 1065 2620 513 Size: 552 1555 4035 Fragments arranged by size: 4035 1555 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3280 487 Size: 683 56 2054 3349 Fragments arranged by size: 3349 2054 683 56 BtsI GCAGTG_nn' Cuts at: 2513 2943 3658 4452 4472 2513 Size: 430 715 794 20 4183 Fragments arranged by size: 4183 794 715 430 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6142 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6107 DraI TTT'AAA Cuts at: 3446 3446 Size: 6142 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5149 809 Size: 507 3833 1802 Fragments arranged by size: 3833 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2448 2835 2996 Size: 54 1425 50 275 18 618 387 161 Cuts at: 2996 3589 4478 6107 8 Size: 593 889 1629 43 Fragments arranged by size: 1629 1425 889 618 593 387 275 161 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2996 3589 1537 Size: 275 1184 593 4090 Fragments arranged by size: 4090 1184 593 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2145 2699 4072 1353 Size: 792 554 1373 3423 Fragments arranged by size: 3423 1373 792 554 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2243 4729 5103 5249 387 Size: 1064 138 654 2486 374 146 1280 Fragments arranged by size: 2486 1280 1064 654 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2370 2723 4205 4802 5589 5857 1239 Size: 393 738 353 1482 597 787 268 1524 Fragments arranged by size: 1524 1482 787 738 597 393 353 268 EcoO109I rG'GnC_Cy Cuts at: 2712 2712 Size: 6142 EcoRI G'AATT_C Cuts at: 3451 3829 3451 Size: 378 5764 FspI TGC'GCA Cuts at: 4648 4648 Size: 6142 HaeII r_GCGC'y Cuts at: 1678 5289 1678 Size: 3611 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2366 2398 2767 2793 2799 Size: 32 135 32 794 32 369 26 6 Cuts at: 2799 2811 2825 2843 4783 4815 4857 4889 1373 Size: 12 14 18 1940 32 42 32 2626 Fragments arranged by size: 2626 1940 794 369 135 42 32 32 32 32 32 26 18 14 12 6 HincII GTy'rAC Cuts at: 669 1657 1938 2731 2952 3486 3732 669 Size: 988 281 793 221 534 246 3079 Fragments arranged by size: 3079 988 793 534 281 246 221 HindIII A'AGCT_T Cuts at: 748 748 Size: 6142 HpaI GTT'AAC Cuts at: 3732 3732 Size: 6142 KpnI G_GTAC'C Cuts at: 3467 3467 Size: 6142 MfeI C'AATT_G Cuts at: 3741 3741 Size: 6142 MluI A'CGCG_T Cuts at: 6018 6018 Size: 6142 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5256 5440 1305 Size: 3951 184 2007 Fragments arranged by size: 3951 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2837 10 Size: 54 1425 343 1005 3315 Fragments arranged by size: 3315 1425 1005 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2405 2960 2963 2979 3401 4100 4459 Size: 648 1239 555 3 16 422 699 359 Cuts at: 4459 4618 518 Size: 159 2042 Fragments arranged by size: 2042 1239 699 648 555 422 359 159 16 3 MspA1I CmG'CkG Cuts at: 1606 2577 2633 3558 4235 5383 5628 1606 Size: 971 56 925 677 1148 245 2120 Fragments arranged by size: 2120 1148 971 925 677 245 56 NaeI GCC'GGC Cuts at: 2877 2877 Size: 6142 NcoI C'CATG_G Cuts at: 513 1065 2620 513 Size: 552 1555 4035 Fragments arranged by size: 4035 1555 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6142 NgoMIV G'CCGG_C Cuts at: 2875 2875 Size: 6142 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6142 NotI GC'GGCC_GC Cuts at: 3589 3589 Size: 6142 NspI r_CATG'y Cuts at: 1132 1229 2629 3040 3500 5045 1132 Size: 97 1400 411 460 1545 2229 Fragments arranged by size: 2229 1545 1400 460 411 97 PasI CC'CwG_GG Cuts at: 1348 2815 1348 Size: 1467 4675 PciI A'CATG_T Cuts at: 3036 5041 3036 Size: 2005 4137 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 5240 PfoI T'CCnGG_A Cuts at: 2528 2528 Size: 6142 PmeI GTTT'AAAC Cuts at: 3446 3446 Size: 6142 PmlI CAC'GTG Cuts at: 2283 2283 Size: 6142 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4211 4243 5724 5756 4211 Size: 32 1481 32 4597 Fragments arranged by size: 4597 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2712 2712 Size: 6142 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6142 PsiI TTA'TAA Cuts at: 3712 3712 Size: 6142 PstI C_TGCA'G Cuts at: 830 2074 2187 3494 830 Size: 1244 113 1307 3478 Fragments arranged by size: 3478 1307 1244 113 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6142 SalI G'TCGA_C Cuts at: 1936 3484 1936 Size: 1548 4594 SapI GCTCTTCn'nnn_ Cuts at: 2145 2145 Size: 6142 SbfI CC_TGCA'GG Cuts at: 3494 3494 Size: 6142 ScaI AGT'ACT Cuts at: 4390 6102 4390 Size: 1712 4430 SfcI C'TryA_G Cuts at: 826 1045 2070 2183 3490 4625 5306 5497 826 Size: 219 1025 113 1307 1135 681 191 1471 Fragments arranged by size: 1471 1307 1135 1025 681 219 191 113 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6142 SmaI CCC'GGG Cuts at: 1872 3469 1872 Size: 1597 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4246 5147 5409 820 Size: 197 926 2303 901 262 1553 Fragments arranged by size: 2303 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3933 493 Size: 3440 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6142 SphI G_CATG'C Cuts at: 1132 2629 3500 1132 Size: 1497 871 3774 Fragments arranged by size: 3774 1497 871 SspI AAT'ATT Cuts at: 5 52 3286 4066 5 Size: 47 3234 780 2081 Fragments arranged by size: 3234 2081 780 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6142 StyI C'CwwG_G Cuts at: 513 1065 2568 2620 3333 3581 513 Size: 552 1503 52 713 248 3074 Fragments arranged by size: 3074 1503 713 552 248 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2304 3056 3387 4169 4186 4339 4524 Size: 1255 724 752 331 782 17 153 185 Cuts at: 4524 4969 325 Size: 445 1498 Fragments arranged by size: 1498 1255 782 752 724 445 331 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3264 3291 4388 Size: 274 80 33 51 1443 1287 27 1097 Cuts at: 4388 6100 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1287 1097 274 138 80 51 33 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2314 2346 6102 6134 2314 Size: 32 3756 32 2322 Fragments arranged by size: 3756 2322 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2354 1316 Size: 1038 5104 XbaI T'CTAG_A Cuts at: 3478 3478 Size: 6142 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6142 XmaI C'CCGG_G Cuts at: 1870 3467 1870 Size: 1597 4545 XmnI GAAnn'nnTTC Cuts at: 4271 4271 Size: 6142 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3950 276 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI