Restriction map for
pFN21AB8443
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BbrPI
BcgI BlnI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HgaI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MslI
MunI MvnI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI StuI SwaI Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AciI 1689 GCGG CCGC(-3/-1)
AcsI 953 AAATTT [GA]^AATT[CT]
AcyI 1302 GGCGCC G[GA]^CG[CT]C
AfaI 1137 GTAC GT^AC
AflII 1569 CTTAAG C^TTAAG
AflIII 853 ACATGT A^C[GA][CT]GT
ApaI 290 GGGCCC GGGCC^C
ApoI 953 AAATTT [GA]^AATT[CT]
AspHI 180 GAGCTC G[AT]GC[AT]^C
AvaI 5 CTCGGG C^[CT]CG[GA]G
BalI 330 TGGCCA TGG^CCA
BamHI 1536 GGATCC G^GATCC
BbeI 1302 GGCGCC GGCGC^C
BbsI 1594 GAAGAC GAAGAC(2/6)
BbvCI 383 CCTCAGC CCTCAGC(-5/-2)
BciVI 1398 GTATCC GTATCC(6/5)
BclI 750 TGATCA T^GATCA
BcnI 428 CCCGG CC^[GC]GG
BfrI 1569 CTTAAG C^TTAAG
BglI 271 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1617 AGATCT A^GATCT
BlpI 25 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 25 GCTCAGC GC^T[ACGT]AGC
BpuAI 1594 GAAGAC GAAGAC(2/6)
BsaHI 1302 GGCGCC G[GA]^CG[CT]C
BsgI 1132 GTGCAG GTGCAG(16/14)
BsiHKAI 180 GAGCTC G[AT]GC[AT]^C
BsoBI 5 CTCGGG C^[CT]CG[GA]G
BspLU11I 853 ACATGT A^CATGT
BspMI 1096 ACCTGC ACCTGC(4/8)
CelII 25 GCTCAGC GC^T[ACGT]AGC
DrdI 1422 GACTCAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 750 TGATCA T^GATCA
HapII 429 CCGG C^CGG
Hin1I 1302 GGCGCC G[GA]^CG[CT]C
HpaII 429 CCGG C^CGG
HphI 1448 TCACC GGTGA(8/7)
KasI 1302 GGCGCC G^GCGCC
MaeII 1548 ACGT A^CGT
MluNI 330 TGGCCA TGG^CCA
MscI 330 TGGCCA TGG^CCA
MspA1I 1736 CAGCTG C[AC]G^C[GT]G
MspI 429 CCGG C^CGG
NarI 1302 GGCGCC GG^CGCC
NciI 428 CCCGG CC^[GC]GG
NcoI 63 CCATGG C^CATGG
NspI 853 ACATGT [GA]CATG^[CT]
PpuMI 579 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 1736 CAGCTG CAG^CTG
RsaI 1137 GTAC GT^AC
SacI 180 GAGCTC GAGCT^C
SfcI 743 CTGTAG C^T[GA][CT]AG
SfoI 1302 GGCGCC GGC^GCC
SpeI 1678 ACTAGT A^CTAGT
SspI 494 AATATT AAT^ATT
TfiI 1639 GAATC G^A[AT]TC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 45 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
323 CAGAGTCTG
BfaI 1663 CTAG C^TAG
1679 CTAG
BsaI 1258 GAGACC GGTCTC(1/5)
1720 GAGACC
BspHI 1143 TCATGA T^CATGA
1268 TCATGA
BsrDI 597 CATTGC GCAATG(2/0)
623 GCAATG
BstYI 1536 GGATCC [GA]^GATC[CT]
1617 AGATCT
Cac8I 834 GCTGGC GC[ACGT]^[ACGT]GC
1100 GCAAGC
CfoI 1303 GCGC GCG^C
1731 GCGC
DraII 290 GGGCCCT [GA]G^G[ACGT]CC[CT]
579 GGGTCCT
DsaI 63 CCATGG C^C[GA][CT]GG
1579 CCGTGG
EaeI 330 TGGCCA [CT]^GGCC[GA]
430 CGGCCA
EarI 1204 GAAGAG CTCTTC(1/4)
1602 GAAGAG
EcoO109I 290 GGGCCCT [GA]G^G[ACGT]CC[CT]
579 GGGTCCT
EcoT14I 63 CCATGG C^C[AT][AT]GG
84 CCAAGG
HaeII 1302 GGCGCC [GA]GCGC^[CT]
1730 AGCGCC
HhaI 1303 GCGC GCG^C
1731 GCGC
HinP1I 1303 GCGC G^CGC
1731 GCGC
Ksp632I 1204 GAAGAG CTCTTC(1/4)
1602 GAAGAG
MaeI 1663 CTAG C^TAG
1679 CTAG
MflI 1536 GGATCC [GA]^GATC[CT]
1617 AGATCT
RcaI 1143 TCATGA T^CATGA
1268 TCATGA
StyI 63 CCATGG C^C[AT][AT]GG
84 CCAAGG
XhoII 1536 GGATCC [GA]^GATC[CT]
1617 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AluI AlwI AocI AspI AvaII
BanI BanII BbvI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI Bsu36I Cfr13I DdeI
DpnII Eco81I EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PflFI PleI
Sau3AI Sau96I ScrFI SfaNI SmlI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I