Restriction map for
pFN21AB6380
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BlnI BpmI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp45I TspRI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 7 GAATTC [GA]^AATT[CT]
AlwNI 239 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 7 GAATTC [GA]^AATT[CT]
Asp700I 822 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 714 GAGCAC G[AT]GC[AT]^C
AvaII 364 GGACC G^G[AT]CC
BalI 782 TGGCCA TGG^CCA
BanI 694 GGTGCC G^G[CT][GA]CC
BcnI 641 CCGGG CC^[GC]GG
BfaI 460 CTAG C^TAG
BglII 870 AGATCT A^GATCT
BlpI 879 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 879 GCTGAGC GC^T[ACGT]AGC
BsaAI 52 TACGTG [CT]AC^GT[GA]
BsiEI 834 CGACCG CG[GA][CT]^CG
BsiHKAI 714 GAGCAC G[AT]GC[AT]^C
BsmFI 363 GGGAC GGGAC(10/14)
BsmI 170 GCATTC GAATGC(1/-1)
BstYI 870 AGATCT [GA]^GATC[CT]
Cac8I 343 GCCAGC GC[ACGT]^[ACGT]GC
CelII 879 GCTGAGC GC^T[ACGT]AGC
EaeI 782 TGGCCA [CT]^GGCC[GA]
EcoRI 7 GAATTC G^AATTC
HapII 641 CCGG C^CGG
HgaI 274 GACGC GACGC(5/10)
HpaII 641 CCGG C^CGG
MaeI 460 CTAG C^TAG
MaeIII 532 GTAAC ^GT[ACGT]AC
MflI 870 AGATCT [GA]^GATC[CT]
MluNI 782 TGGCCA TGG^CCA
MscI 782 TGGCCA TGG^CCA
MslI 495 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 641 CCGG C^CGG
MwoI 668 GCATGAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 641 CCGGG CC^[GC]GG
NlaIII 669 CATG CATG^
PleI 13 GACTC GAGTC(4/5)
PstI 278 CTGCAG CTGCA^G
SfcI 278 CTGCAG C^T[GA][CT]AG
SmlI 721 CTTGAG C^T[CT][GA]AG
TfiI 241 GAATC G^A[AT]TC
XhoII 870 AGATCT [GA]^GATC[CT]
XmnI 822 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 389 GTAC GT^AC
810 GTAC
AlwI 301 GGATC GGATC(4/5)
843 GGATC
BbsI 19 GAAGAC GAAGAC(2/6)
188 GAAGAC
BbvI 674 GCAGC GCAGC(8/12)
277 GCTGC
BmyI 379 GTGCCC G[AGT]GC[ACT]^C
714 GAGCAC
BpuAI 19 GAAGAC GAAGAC(2/6)
188 GAAGAC
BsaJI 144 CCAAGG C^C[ACGT][ACGT]GG
497 CCAAGG
BsiYI 58 CCGTCGGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
641 CCGGGTCAGGG
BslI 58 CCGTCGGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
641 CCGGGTCAGGG
BsmAI 412 GAGAC GTCTC(1/5)
421 GAGAC
Bsp1286I 379 GTGCCC G[AGT]GC[ACT]^C
714 GAGCAC
BstNI 179 CCAGG CC^[AT]GG
314 CCAGG
EcoRII 179 CCAGG ^CC[AT]GG
314 CCAGG
EcoT14I 144 CCAAGG C^C[AT][AT]GG
497 CCAAGG
Fnu4HI 277 GCTGC GC^[ACGT]GC
674 GCAGC
HinfI 13 GACTC G^A[ACGT]TC
241 GAATC
ItaI 277 GCTGC GC^[ACGT]GC
674 GCAGC
MaeII 31 ACGT A^CGT
53 ACGT
MvaI 179 CCAGG CC^[AT]GG
314 CCAGG
NlaIV 363 GGGACC GG[ACGT]^[ACGT]CC
694 GGTGCC
RsaI 389 GTAC GT^AC
810 GTAC
SapI 124 GAAGAGC GCTCTTC(1/4)
454 GAAGAGC
StyI 144 CCAAGG C^C[AT][AT]GG
497 CCAAGG
TseI 277 GCTGC G^C[AT]GC
674 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI BseRI BsrI BssKI BstF5I
Cfr13I DdeI DpnII EarI FokI
HaeIII HphI Ksp632I MboI MboII
MnlI MseI NdeII Sau3AI Sau96I
ScrFI SfaNI TaqI Tru9I Tsp509I
TthHB8I