Restriction map for
pFN21AE0486
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 442 CGCG CG^CG
ApaI 519 GGGCCC GGGCC^C
BalI 462 TGGCCA TGG^CCA
BanII 519 GGGCCC G[GA]GC[CT]^C
BbrPI 466 CACGTG CAC^GTG
BglI 418 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 452 AGATCT A^GATCT
BsaAI 466 CACGTG [CT]AC^GT[GA]
BseRI 244 GAGGAG GAGGAG(10/8)
BsmI 332 GCATTC GAATGC(1/-1)
BspMI 491 ACCTGC ACCTGC(4/8)
BsrFI 338 ACCGGC [GA]^CCGG[CT]
BsrI 167 CCAGT ACTGG(1/-1)
BstUI 442 CGCG CG^CG
BstXI 50 CCATCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 452 AGATCT [GA]^GATC[CT]
Cfr10I 338 ACCGGC [GA]^CCGG[CT]
DraIII 343 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 77 CTCTTC CTCTTC(1/4)
EcoT14I 422 CCATGG C^C[AT][AT]GG
FauI 315 GCGGG CCCGC(4/6)
HaeII 431 AGCGCC [GA]GCGC^[CT]
Ksp632I 77 CTCTTC CTCTTC(1/4)
MaeII 467 ACGT A^CGT
MaeIII 392 GTGAC ^GT[ACGT]AC
MflI 452 AGATCT [GA]^GATC[CT]
MluNI 462 TGGCCA TGG^CCA
MscI 462 TGGCCA TGG^CCA
MseI 209 TTAA T^TAA
MspA1I 88 CAGCTG C[AC]G^C[GT]G
MvnI 442 CGCG CG^CG
NcoI 422 CCATGG C^CATGG
PmaCI 466 CACGTG CAC^GTG
PmlI 466 CACGTG CAC^GTG
PpuMI 533 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 88 CAGCTG CAG^CTG
SapI 76 GCTCTTC GCTCTTC(1/4)
StyI 422 CCATGG C^C[AT][AT]GG
TaqI 100 TCGA T^CGA
TfiI 174 GAATC G^A[AT]TC
Tru9I 209 TTAA T^TAA
Tsp45I 392 GTGAC ^GT[GC]AC
Tsp509I 186 AATT ^AATT
TthHB8I 100 TCGA T^CGA
XhoII 452 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 94 GCGG CCGC(-3/-1)
315 GCGG
AvaII 135 GGTCC G^G[AT]CC
534 GGACC
BcnI 31 CCCGG CC^[GC]GG
472 CCCGG
BsmAI 230 GTCTC GTCTC(1/5)
478 GTCTC
BstF5I 72 GGATG GGATG(2/0)
264 GGATG
DpnII 195 GATC ^GATC
453 GATC
DsaI 347 CCGTGG C^C[GA][CT]GG
422 CCATGG
EaeI 44 CGGCCA [CT]^GGCC[GA]
462 TGGCCA
FokI 72 GGATG GGATG(9/13)
264 GGATG
HphI 351 GGTGA GGTGA(8/7)
336 TCACC
MboI 195 GATC ^GATC
453 GATC
MslI 259 CATGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
345 CACCGTGGTG
NciI 31 CCCGG CC^[GC]GG
472 CCCGG
NdeII 195 GATC ^GATC
453 GATC
NlaIII 259 CATG CATG^
423 CATG
PleI 97 GACTC GAGTC(4/5)
247 GAGTC
Sau3AI 195 GATC ^GATC
453 GATC
TspRI 168 CAGTG CA[GC]TG[ACGT][ACGT]^
325 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI AlwNI BbvI BmyI BpmI
BsaJI BsiYI BslI BsmFI Bsp1286I
BssKI BstNI Cac8I CfoI Cfr13I
DdeI DraII EcoO109I EcoRII Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MboII MnlI MspI
MvaI MwoI NlaIV PstI Sau96I
ScrFI SfaNI SfcI TseI XcmI