(Circular) (Six-base) MAPSORT of: pFN21AE0486.seq Check: 7413 from: 1 to: 5245 pFN21AE0486 5245 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3055 278 Size: 53 83 186 936 1519 2468 Fragments arranged by size: 2468 1519 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2588 1937 Size: 651 4594 Acc65I G'GTAC_C Cuts at: 2566 2566 Size: 5245 AclI AA'CG_TT Cuts at: 1563 3372 3745 1563 Size: 1809 373 3063 Fragments arranged by size: 3063 1809 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2156 3308 4692 4960 1632 Size: 524 1152 1384 268 1917 Fragments arranged by size: 1917 1384 1152 524 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5048 AflIII A'CryG_T Cuts at: 1203 4144 5121 1203 Size: 2941 977 1327 Fragments arranged by size: 2941 1327 977 AgeI A'CCGG_T Cuts at: 4076 4076 Size: 5245 AhdI GACnn_n'nnGTC Cuts at: 3974 3974 Size: 5245 AleI CACnn'nnGTG Cuts at: 1166 2354 1166 Size: 1188 4057 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2478 1184 Size: 1294 3951 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2486 2518 2486 Size: 32 5213 AlwNI CAG_nnn'CTG Cuts at: 2095 2315 2440 2988 4560 2095 Size: 220 125 548 1572 2780 Fragments arranged by size: 2780 1572 548 220 125 ApaI G_GGCC'C Cuts at: 2528 2528 Size: 5245 ApaLI G'TGCA_C Cuts at: 3302 4458 3302 Size: 1156 4089 ApoI r'AATT_y Cuts at: 1493 2554 2780 2932 5041 1493 Size: 1061 226 152 2109 1697 Fragments arranged by size: 2109 1697 1061 226 152 AseI AT'TA_AT Cuts at: 160 1032 3799 160 Size: 872 2767 1606 Fragments arranged by size: 2767 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5245 AvaI C'yCGr_G Cuts at: 1870 1943 2570 1870 Size: 73 627 4545 Fragments arranged by size: 4545 627 73 BamHI G'GATC_C Cuts at: 2575 2942 2575 Size: 367 4878 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2566 4021 618 Size: 325 217 627 779 1455 1842 Fragments arranged by size: 1842 1455 779 627 325 217 BanII G_rGCy'C Cuts at: 1391 2528 2564 1391 Size: 1137 36 4072 Fragments arranged by size: 4072 1137 36 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4274 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3436 3470 1903 Size: 34 1499 34 3678 Fragments arranged by size: 3678 1499 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3137 4347 3137 Size: 1210 4035 BclI T'GATC_A Cuts at: 1548 1548 Size: 5245 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2429 3856 136 Size: 107 122 71 631 605 757 1427 1525 Fragments arranged by size: 1525 1427 757 631 605 122 107 71 BglII A'GATC_T Cuts at: 2457 5240 2457 Size: 2783 2462 BlpI GC'TnA_GC Cuts at: 2662 2662 Size: 5245 Bme1580I G_kGCm'C Cuts at: 1790 2072 2478 2528 3306 4462 1790 Size: 282 406 50 778 1156 2573 Fragments arranged by size: 2573 1156 778 406 282 50 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5245 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2976 3934 442 Size: 416 53 83 399 24 1559 958 1753 Fragments arranged by size: 1753 1559 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5245 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2545 2545 Size: 5245 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2155 2222 2423 2555 3905 1239 Size: 916 67 201 132 1350 2579 Fragments arranged by size: 2579 1350 916 201 132 67 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3370 4235 4533 3370 Size: 865 298 4082 Fragments arranged by size: 4082 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3908 882 Size: 617 2409 2219 Fragments arranged by size: 2409 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2473 3036 493 Size: 711 1269 563 2702 Fragments arranged by size: 2702 1269 711 563 BsaBI GATnn'nnATC Cuts at: 5239 5239 Size: 5245 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3052 3434 4069 275 Size: 53 83 186 936 1519 382 635 1451 Fragments arranged by size: 1519 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3678 4076 4350 4497 4908 3678 Size: 398 274 147 411 4015 Fragments arranged by size: 4015 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2111 2141 1178 Size: 30 165 30 708 30 4282 Fragments arranged by size: 4282 708 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2264 1186 Size: 1078 4167 BseYI C'CCAG_C Cuts at: 2315 4448 4944 2315 Size: 2133 496 2616 Fragments arranged by size: 2616 2133 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4672 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2695 3456 4484 1540 Size: 275 186 694 761 1028 2301 Fragments arranged by size: 2301 1028 761 694 275 186 BsiHKAI G_wGCw'C Cuts at: 3306 3391 4462 3306 Size: 85 1071 4089 Fragments arranged by size: 4089 1071 85 BsmI GAATG_Cn' Cuts at: 2337 2756 2849 2337 Size: 419 93 4733 Fragments arranged by size: 4733 419 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2478 2528 2564 3306 3391 4462 Size: 399 282 406 50 36 742 85 1071 Cuts at: 4462 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 406 399 282 85 50 36 BspEI T'CCGG_A Cuts at: 4908 4908 Size: 5245 BspHI T'CATG_A Cuts at: 3134 4864 3134 Size: 1730 3515 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2505 2600 844 Size: 1661 95 3489 Fragments arranged by size: 3489 1661 95 BsrBI CCG'CTC Cuts at: 1394 1823 3132 1394 Size: 429 1309 3507 Fragments arranged by size: 3507 1309 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3740 3914 65 Size: 1183 2492 174 1396 Fragments arranged by size: 2492 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2343 3889 4076 1700 Size: 110 533 1546 187 2869 Fragments arranged by size: 2869 1546 533 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5245 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5245 BssSI C'ACGA_G Cuts at: 3305 4317 3305 Size: 1012 4233 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5245 BstBI TT'CG_AA Cuts at: 4049 4049 Size: 5245 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5245 BstXI CCAn_nnnn'nTGG Cuts at: 2062 2062 Size: 5245 BstYI r'GATC_y Cuts at: 1972 2457 2575 2942 3327 3344 4796 4882 4894 Size: 485 118 367 385 17 1452 86 12 Cuts at: 4894 5240 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 485 385 367 346 118 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2352 2427 513 Size: 552 1287 75 3331 Fragments arranged by size: 3331 1287 552 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4506 Fragments arranged by size: 4506 683 56 BtsI GCAGTG_nn' Cuts at: 2329 2761 3555 3575 2329 Size: 432 794 20 3999 Fragments arranged by size: 3999 794 432 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5245 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5210 DraI TTT'AAA Cuts at: 2549 2549 Size: 5245 DraIII CAC_nnn'GTG Cuts at: 2353 2353 Size: 5245 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4252 809 Size: 507 2936 1802 Fragments arranged by size: 2936 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 2467 2692 Size: 54 1425 50 275 18 219 418 225 Cuts at: 2692 3581 5210 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 418 275 225 219 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2692 1537 Size: 275 880 4090 Fragments arranged by size: 4090 880 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2088 3175 1353 Size: 735 1087 3423 Fragments arranged by size: 3423 1087 735 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2114 3832 4206 4352 387 Size: 1064 138 525 1718 374 146 1280 Fragments arranged by size: 1718 1280 1064 525 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2155 2156 2222 2423 2555 3308 3905 Size: 393 523 1 66 201 132 753 597 Cuts at: 3905 4692 4960 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 753 597 523 393 268 201 132 66 1 EcoO109I rG'GnC_Cy Cuts at: 2019 2524 2525 2539 2019 Size: 505 1 14 4725 Fragments arranged by size: 4725 505 14 1 EcoRI G'AATT_C Cuts at: 2554 2932 2554 Size: 378 4867 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2161 2161 Size: 5245 FspI TGC'GCA Cuts at: 3751 3751 Size: 5245 HaeII r_GCGC'y Cuts at: 1678 2440 4392 1678 Size: 762 1952 2531 Fragments arranged by size: 2531 1952 762 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2111 2143 3886 3918 3960 Size: 32 135 32 539 32 1743 32 42 Cuts at: 3960 3992 1373 Size: 32 2626 Fragments arranged by size: 2626 1743 539 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2589 2835 669 Size: 988 281 651 246 3079 Fragments arranged by size: 3079 988 651 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5245 HpaI GTT'AAC Cuts at: 2835 2835 Size: 5245 KpnI G_GTAC'C Cuts at: 2570 2570 Size: 5245 MfeI C'AATT_G Cuts at: 2844 2844 Size: 5245 MluI A'CGCG_T Cuts at: 5121 5121 Size: 5245 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4359 4543 1305 Size: 3054 184 2007 Fragments arranged by size: 3054 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2469 10 Size: 54 1425 343 637 2786 Fragments arranged by size: 2786 1425 637 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2268 2354 3203 3562 3721 518 Size: 648 843 259 86 849 359 159 2042 Fragments arranged by size: 2042 849 843 648 359 259 159 86 MspA1I CmG'CkG Cuts at: 1606 2095 2661 3338 4486 4731 1606 Size: 489 566 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 566 489 245 NcoI C'CATG_G Cuts at: 513 1065 2427 513 Size: 552 1362 3331 Fragments arranged by size: 3331 1362 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5245 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5245 NotI GC'GGCC_GC Cuts at: 2692 2692 Size: 5245 NspI r_CATG'y Cuts at: 1132 1229 2603 4148 1132 Size: 97 1374 1545 2229 Fragments arranged by size: 2229 1545 1374 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5245 PciI A'CATG_T Cuts at: 4144 4144 Size: 5245 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5245 PmeI GTTT'AAAC Cuts at: 2549 2549 Size: 5245 PmlI CAC'GTG Cuts at: 2473 2473 Size: 5245 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3314 3346 4827 4859 3314 Size: 32 1481 32 3700 Fragments arranged by size: 3700 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2539 2539 Size: 5245 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5245 PsiI TTA'TAA Cuts at: 2815 2815 Size: 5245 PspOMI G'GGCC_C Cuts at: 2524 2524 Size: 5245 PstI C_TGCA'G Cuts at: 830 2091 2336 2457 2597 830 Size: 1261 245 121 140 3478 Fragments arranged by size: 3478 1261 245 140 121 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5245 PvuII CAG'CTG Cuts at: 2095 2095 Size: 5245 SalI G'TCGA_C Cuts at: 1936 2587 1936 Size: 651 4594 SapI GCTCTTCn'nnn_ Cuts at: 2088 2088 Size: 5245 SbfI CC_TGCA'GG Cuts at: 2597 2597 Size: 5245 ScaI AGT'ACT Cuts at: 3493 5205 3493 Size: 1712 3533 SexAI A'CCwGG_T Cuts at: 2541 2541 Size: 5245 SfcI C'TryA_G Cuts at: 826 1045 2087 2332 2453 2593 3728 4409 4600 Size: 219 1042 245 121 140 1135 681 191 Cuts at: 4600 826 Size: 1471 Fragments arranged by size: 1471 1135 1042 681 245 219 191 140 121 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5245 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5245 SmaI CCC'GGG Cuts at: 1872 2572 1872 Size: 700 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3349 4250 4512 820 Size: 197 926 1406 901 262 1553 Fragments arranged by size: 1553 1406 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3036 493 Size: 2543 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5245 SphI G_CATG'C Cuts at: 1132 2603 1132 Size: 1471 3774 SspI AAT'ATT Cuts at: 5 52 3169 5 Size: 47 3117 2081 Fragments arranged by size: 3117 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5245 StyI C'CwwG_G Cuts at: 513 1065 2427 2684 513 Size: 552 1362 257 3074 Fragments arranged by size: 3074 1362 552 257 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3272 3289 3442 3627 4072 325 Size: 1255 1692 17 153 185 445 1498 Fragments arranged by size: 1692 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3491 5203 96 Size: 274 80 33 51 1443 1514 1712 138 Fragments arranged by size: 1712 1514 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5205 5237 5205 Size: 32 5213 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5245 XbaI T'CTAG_A Cuts at: 2581 2581 Size: 5245 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2059 2062 2281 2059 Size: 3 219 5023 Fragments arranged by size: 5023 219 3 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5245 XmaI C'CCGG_G Cuts at: 1870 2570 1870 Size: 700 4545 XmnI GAAnn'nnTTC Cuts at: 3374 3374 Size: 5245 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3053 276 Size: 53 83 186 936 1519 2468 Fragments arranged by size: 2468 1519 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI