Restriction map for
pFN21AE0486
(Linear) (Six-base) MAPSORT of: KIEE0486.seq Check: 553 from: 1 to: 540
KIEE0486.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 540
Size: 151 389
AleI CACnn'nnGTG
Cuts at: 0 349 540
Size: 349 191
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 473 540
Size: 473 67
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 481 513 540
Size: 481 32 27
Fragments arranged by size:
481 32 27
AlwNI CAG_nnn'CTG
Cuts at: 0 90 310 435 540
Size: 90 220 125 105
Fragments arranged by size:
220 125 105 90
ApaI G_GGCC'C
Cuts at: 0 523 540
Size: 523 17
BanII G_rGCy'C
Cuts at: 0 523 540
Size: 523 17
BglI GCCn_nnn'nGGC
Cuts at: 0 424 540
Size: 424 116
BglII A'GATC_T
Cuts at: 0 452 540
Size: 452 88
Bme1580I G_kGCm'C
Cuts at: 0 67 473 523 540
Size: 67 406 50 17
Fragments arranged by size:
406 67 50 17
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 540
Size: 540
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 217 418 540
Size: 150 67 201 122
Fragments arranged by size:
201 150 122 67
BsaAI yAC'GTr
Cuts at: 0 468 540
Size: 468 72
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 106 136 540
Size: 106 30 404
Fragments arranged by size:
404 106 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 259 540
Size: 259 281
BseYI C'CCAG_C
Cuts at: 0 310 540
Size: 310 230
BsmI GAATG_Cn'
Cuts at: 0 332 540
Size: 332 208
Bsp1286I G_dGCh'C
Cuts at: 0 67 473 523 540
Size: 67 406 50 17
Fragments arranged by size:
406 67 50 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 500 540
Size: 500 40
BsrFI r'CCGG_y
Cuts at: 0 338 540
Size: 338 202
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 540
Size: 57 483
BstYI r'GATC_y
Cuts at: 0 452 540
Size: 452 88
BtgI C'CryG_G
Cuts at: 0 347 422 540
Size: 347 75 118
Fragments arranged by size:
347 118 75
BtsI GCAGTG_nn'
Cuts at: 0 324 540
Size: 324 216
DraIII CAC_nnn'GTG
Cuts at: 0 348 540
Size: 348 192
EaeI y'GGCC_r
Cuts at: 0 44 462 540
Size: 44 418 78
Fragments arranged by size:
418 78 44
EarI CTCTTCn'nnn_
Cuts at: 0 83 540
Size: 83 457
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 109 540
Size: 109 431
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 151 217 418 540
Size: 150 1 66 201 122
Fragments arranged by size:
201 150 122 66 1
EcoO109I rG'GnC_Cy
Cuts at: 0 14 519 520 534 540
Size: 14 505 1 14 6
Fragments arranged by size:
505 14 14 6 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 156 540
Size: 156 384
HaeII r_GCGC'y
Cuts at: 0 435 540
Size: 435 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 540
Size: 106 32 402
Fragments arranged by size:
402 106 32
MscI TGG'CCA
Cuts at: 0 464 540
Size: 464 76
MslI CAynn'nnrTG
Cuts at: 0 263 349 540
Size: 263 86 191
Fragments arranged by size:
263 191 86
MspA1I CmG'CkG
Cuts at: 0 90 540
Size: 90 450
NcoI C'CATG_G
Cuts at: 0 422 540
Size: 422 118
PmlI CAC'GTG
Cuts at: 0 468 540
Size: 468 72
PpuMI rG'GwC_Cy
Cuts at: 0 534 540
Size: 534 6
PspOMI G'GGCC_C
Cuts at: 0 519 540
Size: 519 21
PstI C_TGCA'G
Cuts at: 0 86 331 452 540
Size: 86 245 121 88
Fragments arranged by size:
245 121 88 86
PvuII CAG'CTG
Cuts at: 0 90 540
Size: 90 450
SapI GCTCTTCn'nnn_
Cuts at: 0 83 540
Size: 83 457
SfcI C'TryA_G
Cuts at: 0 82 327 448 540
Size: 82 245 121 92
Fragments arranged by size:
245 121 92 82
StyI C'CwwG_G
Cuts at: 0 422 540
Size: 422 118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 54 57 276 540
Size: 54 3 219 264
Fragments arranged by size:
264 219 54 3
Enzymes that do cut:
AcuI AleI AlfI AloI AlwNI ApaI BanII BglI
BglII Bme1580I BplI BpmI BsaAI BsaXI BseRI BseYI
BsmI Bsp1286I BspMI BsrFI BstXI BstYI BtgI BtsI
DraIII EaeI EarI EciI Eco57MI EcoO109I FalI HaeII
Hin4I MscI MslI MspA1I NcoI PmlI PpuMI PspOMI
PstI PvuII SapI SfcI StyI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BlpI BmgBI BmrI BmtI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsgI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EagI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI