Restriction map for
pFN21AB9881
##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AvrII BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I ClaI CpoI DraI Eam1105I
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FbaI HincII HindII HindIII HpaI
MamI MfeI MluI MseI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI SexAI SfuI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tth111I XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 1667 GGTACC G^GTACC
AccIII 69 TCCGGA T^CCGGA
AflIII 569 ACGTGT A^C[GA][CT]GT
AocI 1061 CCTCAGG CC^T[ACGT]AGG
Asp718I 1667 GGTACC G^GTACC
BalI 821 TGGCCA TGG^CCA
BamHI 1056 GGATCC G^GATCC
BbsI 1579 GAAGAC GAAGAC(2/6)
BbvCI 1417 GCTGAGG CCTCAGC(-5/-2)
BfaI 1045 CTAG C^TAG
BlpI 123 GCTGAGC GC^T[ACGT]AGC
BpmI 973 CTGGAG CTGGAG(16/14)
Bpu1102I 123 GCTGAGC GC^T[ACGT]AGC
BpuAI 1579 GAAGAC GAAGAC(2/6)
BsaWI 69 TCCGGA [AT]^CCGG[AT]
BseAI 69 TCCGGA T^CCGGA
BspEI 69 TCCGGA T^CCGGA
Bsu36I 1061 CCTCAGG CC^T[ACGT]AGG
CelII 123 GCTGAGC GC^T[ACGT]AGC
DrdI 647 GACGGCCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1061 CCTCAGG CC^T[ACGT]AGG
EcoNI 75 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 885 GGCCGGCC GGCCGG^CC
KpnI 1667 GGTACC GGTAC^C
MaeI 1045 CTAG C^TAG
MluNI 821 TGGCCA TGG^CCA
MroI 69 TCCGGA T^CCGGA
MscI 821 TGGCCA TGG^CCA
MslI 846 CACCCGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 930 GCATGT [GA]CATG^[CT]
PaeR7I 229 CTCGAG C^TCGAG
PpuMI 872 GGGTCCT [GA]G^G[AT]CC[CT]
SacI 1395 GAGCTC GAGCT^C
ScaI 827 AGTACT AGT^ACT
SfiI 757 GGCCAAGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 379 CGCCGGCG C[GA]^CCGG[CT]G
Tsp509I 1322 AATT ^AATT
XhoI 229 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 667 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
814 CAGGCCCTG
ApaI 3 GGGCCC GGGCC^C
1427 GGGCCC
AviII 77 TGCGCA TGC^GCA
314 TGCGCA
BbeI 383 GGCGCC GGCGC^C
456 GGCGCC
BsaI 586 GAGACC GGTCTC(1/5)
1078 GGTCTC
BstYI 260 AGATCC [GA]^GATC[CT]
1056 GGATCC
DraIII 388 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1096 CACTTTGTG
FspI 77 TGCGCA TGC^GCA
314 TGCGCA
KasI 383 GGCGCC G^GCGCC
456 GGCGCC
KspI 473 CCGCGG CCGC^GG
623 CCGCGG
MaeII 25 ACGT A^CGT
569 ACGT
MflI 260 AGATCC [GA]^GATC[CT]
1056 GGATCC
NarI 383 GGCGCC GG^CGCC
456 GGCGCC
PleI 1525 GACTC GAGTC(4/5)
862 GAGTC
SacII 473 CCGCGG CCGC^GG
623 CCGCGG
SfaNI 290 GCATC GCATC(5/9)
966 GCATC
SfoI 383 GGCGCC GGC^GCC
456 GGCGCC
StuI 836 AGGCCT AGG^CCT
1065 AGGCCT
TaqI 230 TCGA T^CGA
1776 TCGA
TfiI 66 GAATC G^A[AT]TC
1531 GATTC
TthHB8I 230 TCGA T^CGA
1776 TCGA
XcmI 356 CCAACGGCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
809 CCACCCAGGCCCTGG
XhoII 260 AGATCC [GA]^GATC[CT]
1056 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BanI
BanII BbvI BcnI BglI BmyI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsoBI
Bsp1286I BspMI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PflMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfcI SmlI
StyI TseI Tsp45I TspRI Van91I