(Circular) (Six-base) MAPSORT of: pFN21AB9881.seq Check: 6696 from: 1 to: 6502 pFN21AB9881 6502 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2204 2989 2204 Size: 785 5717 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4312 278 Size: 53 83 186 936 2776 2468 Fragments arranged by size: 2776 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3845 1937 Size: 1908 4594 Acc65I G'GTAC_C Cuts at: 3672 3823 3672 Size: 151 6351 AclI AA'CG_TT Cuts at: 1563 4629 5002 1563 Size: 3066 373 3063 Fragments arranged by size: 3066 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2162 4565 5949 6217 1632 Size: 530 2403 1384 268 1917 Fragments arranged by size: 2403 1917 1384 530 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6305 AflIII A'CryG_T Cuts at: 1203 2574 5401 6378 1203 Size: 1371 2827 977 1327 Fragments arranged by size: 2827 1371 1327 977 AgeI A'CCGG_T Cuts at: 5333 5333 Size: 6502 AhdI GACnn_n'nnGTC Cuts at: 5231 5231 Size: 6502 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6502 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6502 AlwNI CAG_nnn'CTG Cuts at: 2677 2824 4245 5817 2677 Size: 147 1421 1572 3362 Fragments arranged by size: 3362 1572 1421 147 ApaI G_GGCC'C Cuts at: 2012 3436 2012 Size: 1424 5078 ApaLI G'TGCA_C Cuts at: 4559 5715 4559 Size: 1156 5346 ApoI r'AATT_y Cuts at: 1493 3811 4037 4189 6298 1493 Size: 2318 226 152 2109 1697 Fragments arranged by size: 2318 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5056 160 Size: 872 4024 1606 Fragments arranged by size: 4024 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6502 AvaI C'yCGr_G Cuts at: 1870 1943 2234 2526 2923 3827 1870 Size: 73 291 292 397 904 4545 Fragments arranged by size: 4545 904 397 292 291 73 BamHI G'GATC_C Cuts at: 3061 3832 4199 3061 Size: 771 367 5364 Fragments arranged by size: 5364 771 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2388 2461 2482 2963 3476 Size: 325 217 627 601 73 21 481 513 Cuts at: 3476 3619 3672 3823 5278 618 Size: 143 53 151 1455 1842 Fragments arranged by size: 1842 1455 627 601 513 481 325 217 151 143 73 53 21 BanII G_rGCy'C Cuts at: 1391 2012 3404 3436 3821 1391 Size: 621 1392 32 385 4072 Fragments arranged by size: 4072 1392 621 385 32 BbeI G_GCGC'C Cuts at: 2392 2465 2392 Size: 73 6429 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3591 928 Size: 971 1692 3839 Fragments arranged by size: 3839 1692 971 BbvCI CC'TCA_GC Cuts at: 3423 3423 Size: 6502 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4693 4727 1903 Size: 34 2756 34 3678 Fragments arranged by size: 3678 2756 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4394 5604 4394 Size: 1210 5292 BclI T'GATC_A Cuts at: 1548 1548 Size: 6502 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2162 2474 2769 Size: 107 122 71 631 605 490 312 295 Cuts at: 2769 3122 3770 5113 136 Size: 353 648 1343 1525 Fragments arranged by size: 1525 1343 648 631 605 490 353 312 295 122 107 71 BglII A'GATC_T Cuts at: 6497 6497 Size: 6502 BlpI GC'TnA_GC Cuts at: 2129 3919 2129 Size: 1790 4712 Bme1580I G_kGCm'C Cuts at: 1790 2012 2487 3436 3481 3500 4563 5719 1790 Size: 222 475 949 45 19 1063 1156 2573 Fragments arranged by size: 2573 1156 1063 949 475 222 45 19 BmgBI CAC'GTC Cuts at: 1318 2575 1318 Size: 1257 5245 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2902 3439 3487 Size: 416 53 83 399 24 1485 537 48 Cuts at: 3487 4233 5191 442 Size: 746 958 1753 Fragments arranged by size: 1753 1485 958 746 537 416 399 83 53 48 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6502 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3418 3421 3418 Size: 3 6499 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2999 5162 1239 Size: 1760 2163 2579 Fragments arranged by size: 2579 2163 1760 Bpu10I CC'TnA_GC Cuts at: 2678 3423 2678 Size: 745 5757 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2288 3017 3191 4627 5492 5790 2288 Size: 729 174 1436 865 298 3000 Fragments arranged by size: 3000 1436 865 729 298 174 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2585 3089 5165 882 Size: 617 1086 504 2076 2219 Fragments arranged by size: 2219 2076 1086 617 504 BsaAI yAC'GTr Cuts at: 493 1204 4293 493 Size: 711 3089 2702 Fragments arranged by size: 3089 2702 711 BsaBI GATnn'nnATC Cuts at: 6496 6496 Size: 6502 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2165 2389 2462 4309 Size: 53 83 186 936 632 224 73 1847 Cuts at: 4309 4691 5326 275 Size: 382 635 1451 Fragments arranged by size: 1847 1451 936 635 632 382 224 186 83 73 53 BsaWI w'CCGG_w Cuts at: 2074 4935 5333 5607 5754 6165 2074 Size: 2861 398 274 147 411 2411 Fragments arranged by size: 2861 2411 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2162 2192 3665 3695 1178 Size: 30 165 30 759 30 1473 30 3985 Fragments arranged by size: 3985 1473 759 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2081 2345 2546 3242 3245 3248 3251 3263 Size: 895 264 201 696 3 3 3 12 Cuts at: 3263 3266 3293 3296 3308 3311 3314 3317 3356 Size: 3 27 3 12 3 3 3 39 Cuts at: 3356 3365 3368 3394 3398 3410 3617 1186 Size: 9 3 26 4 12 207 4071 Fragments arranged by size: 4071 895 696 264 207 201 39 27 26 12 12 12 9 4 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2335 2670 3079 3629 5705 6201 2335 Size: 335 409 550 2076 496 2636 Fragments arranged by size: 2636 2076 550 496 409 335 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2960 3083 3725 1163 Size: 573 1224 123 642 3940 Fragments arranged by size: 3940 1224 642 573 123 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2478 2657 2896 3952 4713 5741 Size: 275 186 477 179 239 1056 761 1028 Cuts at: 5741 1540 Size: 2301 Fragments arranged by size: 2301 1056 1028 761 477 275 239 186 179 BsiHKAI G_wGCw'C Cuts at: 2135 2510 3404 4563 4648 5719 2135 Size: 375 894 1159 85 1071 2918 Fragments arranged by size: 2918 1159 1071 894 375 85 BsmI GAATG_Cn' Cuts at: 4013 4106 4013 Size: 93 6409 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2135 2487 2510 3404 3436 3481 Size: 399 222 123 352 23 894 32 45 Cuts at: 3481 3500 3821 4563 4648 5719 1391 Size: 19 321 742 85 1071 2174 Fragments arranged by size: 2174 1071 894 742 399 352 321 222 123 85 45 32 23 19 BspEI T'CCGG_A Cuts at: 2074 6165 2074 Size: 4091 2411 BspHI T'CATG_A Cuts at: 4391 6121 4391 Size: 1730 4772 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2088 2204 2989 3857 844 Size: 1244 116 785 868 3489 Fragments arranged by size: 3489 1244 868 785 116 BsrBI CCG'CTC Cuts at: 1394 1823 4389 1394 Size: 429 2566 3507 Fragments arranged by size: 3507 2566 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4997 5171 65 Size: 1183 3749 174 1396 Fragments arranged by size: 3749 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2276 2284 2385 2735 2768 2891 3452 Size: 110 466 8 101 350 33 123 561 Cuts at: 3452 5146 5333 1700 Size: 1694 187 2869 Fragments arranged by size: 2869 1694 561 466 350 187 123 110 101 33 8 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6502 BssHII G'CGCG_C Cuts at: 1925 2152 2430 2521 1925 Size: 227 278 91 5906 Fragments arranged by size: 5906 278 227 91 BssSI C'ACGA_G Cuts at: 4562 5574 4562 Size: 1012 5490 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6502 BstBI TT'CG_AA Cuts at: 5306 5306 Size: 6502 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6502 BstYI r'GATC_y Cuts at: 1972 2265 3061 3832 4199 4584 4601 6053 6139 Size: 293 796 771 367 385 17 1452 86 Cuts at: 6139 6151 6497 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 796 771 385 367 346 293 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3067 3067 Size: 6502 BtgI C'CryG_G Cuts at: 513 1065 2004 2392 2478 2628 3039 3645 3660 Size: 552 939 388 86 150 411 606 15 Cuts at: 3660 513 Size: 3355 Fragments arranged by size: 3355 939 606 552 411 388 150 86 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2233 487 Size: 683 56 1007 4756 Fragments arranged by size: 4756 1007 683 56 BtsI GCAGTG_nn' Cuts at: 2503 3132 4018 4812 4832 2503 Size: 629 886 794 20 4173 Fragments arranged by size: 4173 886 794 629 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6502 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6467 DraI TTT'AAA Cuts at: 3806 3806 Size: 6502 DraIII CAC_nnn'GTG Cuts at: 2398 3106 2398 Size: 708 5794 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2658 5509 809 Size: 507 1342 2851 1802 Fragments arranged by size: 2851 1802 1342 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2475 2550 2654 Size: 54 1425 50 275 18 645 75 104 Cuts at: 2654 2733 2745 2826 2889 2893 3042 3949 4838 Size: 79 12 81 63 4 149 907 889 Cuts at: 4838 6467 8 Size: 1629 43 Fragments arranged by size: 1629 1425 907 889 645 275 149 104 81 79 75 63 54 50 43 18 12 4 EagI C'GGCC_G Cuts at: 1537 1812 2475 2654 2893 3949 1537 Size: 275 663 179 239 1056 4090 Fragments arranged by size: 4090 1056 663 275 239 179 EarI CTCTTCn'nnn_ Cuts at: 1353 3219 3240 3252 3270 3333 3375 3546 3561 Size: 1866 21 12 18 63 42 171 15 Cuts at: 3561 4432 1353 Size: 871 3423 Fragments arranged by size: 3423 1866 871 171 63 42 21 18 15 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2372 5089 5463 5609 387 Size: 1064 138 783 2717 374 146 1280 Fragments arranged by size: 2717 1280 1064 783 374 146 138 EcoICRI GAG'CTC Cuts at: 3402 3402 Size: 6502 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2162 2999 4565 5162 5949 6217 1239 Size: 393 530 837 1566 597 787 268 1524 Fragments arranged by size: 1566 1524 837 787 597 530 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2084 2084 Size: 6502 EcoO109I rG'GnC_Cy Cuts at: 2009 2821 2878 3094 2009 Size: 812 57 216 5417 Fragments arranged by size: 5417 812 216 57 EcoRI G'AATT_C Cuts at: 3811 4189 3811 Size: 378 6124 FseI GG_CCGG'CC Cuts at: 2895 2895 Size: 6502 FspI TGC'GCA Cuts at: 2084 2321 5008 2084 Size: 237 2687 3578 Fragments arranged by size: 3578 2687 237 HaeII r_GCGC'y Cuts at: 1678 2107 2318 2392 2465 2586 2677 5649 1678 Size: 429 211 74 73 121 91 2972 2531 Fragments arranged by size: 2972 2531 429 211 121 91 74 73 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5143 5175 5217 5249 1373 Size: 32 135 32 3571 32 42 32 2626 Fragments arranged by size: 3571 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3846 4092 669 Size: 988 281 1908 246 3079 Fragments arranged by size: 3079 1908 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6502 HpaI GTT'AAC Cuts at: 4092 4092 Size: 6502 KasI G'GCGC_C Cuts at: 2388 2461 2388 Size: 73 6429 KpnI G_GTAC'C Cuts at: 3676 3827 3676 Size: 151 6351 MfeI C'AATT_G Cuts at: 4101 4101 Size: 6502 MluI A'CGCG_T Cuts at: 6378 6378 Size: 6502 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2148 2385 5616 5800 1305 Size: 843 237 3231 184 2007 Fragments arranged by size: 3231 2007 843 237 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2828 10 Size: 54 1425 343 996 3684 Fragments arranged by size: 3684 1425 996 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2855 4460 4819 4978 518 Size: 648 1689 1605 359 159 2042 Fragments arranged by size: 2042 1689 1605 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2026 2412 2480 2630 2728 2944 2977 3079 Size: 420 386 68 150 98 216 33 102 Cuts at: 3079 3918 4595 5743 5988 1606 Size: 839 677 1148 245 2120 Fragments arranged by size: 2120 1148 839 677 420 386 245 216 150 102 98 68 33 NaeI GCC'GGC Cuts at: 2278 2387 2737 2770 2893 2278 Size: 109 350 33 123 5887 Fragments arranged by size: 5887 350 123 109 33 NarI GG'CG_CC Cuts at: 2389 2462 2389 Size: 73 6429 NcoI C'CATG_G Cuts at: 513 1065 2004 3039 3645 3660 513 Size: 552 939 1035 606 15 3355 Fragments arranged by size: 3355 1035 939 606 552 15 NdeI CA'TA_TG Cuts at: 387 387 Size: 6502 NgoMIV G'CCGG_C Cuts at: 2276 2385 2735 2768 2891 2276 Size: 109 350 33 123 5887 Fragments arranged by size: 5887 350 123 109 33 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6502 NotI GC'GGCC_GC Cuts at: 3949 3949 Size: 6502 NspI r_CATG'y Cuts at: 1132 1229 2939 3860 5405 1132 Size: 97 1710 921 1545 2229 Fragments arranged by size: 2229 1710 1545 921 97 PasI CC'CwG_GG Cuts at: 1348 3436 1348 Size: 2088 4414 PciI A'CATG_T Cuts at: 5401 5401 Size: 6502 PflMI CCAn_nnn'nTGG Cuts at: 1108 2062 2367 2398 2601 2824 2914 1108 Size: 954 305 31 203 223 90 4696 Fragments arranged by size: 4696 954 305 223 203 90 31 PfoI T'CCnGG_A Cuts at: 2034 2055 3513 2034 Size: 21 1458 5023 Fragments arranged by size: 5023 1458 21 PmeI GTTT'AAAC Cuts at: 3806 3806 Size: 6502 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4571 4603 6084 6116 4571 Size: 32 1481 32 4957 Fragments arranged by size: 4957 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2878 2878 Size: 6502 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6502 PsiI TTA'TAA Cuts at: 4072 4072 Size: 6502 PspOMI G'GGCC_C Cuts at: 2008 3432 2008 Size: 1424 5078 PspXI vC'TCGA_Gb Cuts at: 2234 2234 Size: 6502 PstI C_TGCA'G Cuts at: 830 2411 2866 3078 3854 830 Size: 1581 455 212 776 3478 Fragments arranged by size: 3478 1581 776 455 212 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6502 PvuII CAG'CTG Cuts at: 2026 2944 3079 2026 Size: 918 135 5449 Fragments arranged by size: 5449 918 135 SacI G_AGCT'C Cuts at: 3404 3404 Size: 6502 SacII CC_GC'GG Cuts at: 2481 2631 2481 Size: 150 6352 SalI G'TCGA_C Cuts at: 1936 3844 1936 Size: 1908 4594 SbfI CC_TGCA'GG Cuts at: 3854 3854 Size: 6502 ScaI AGT'ACT Cuts at: 2834 4750 6462 2834 Size: 1916 1712 2874 Fragments arranged by size: 2874 1916 1712 SfcI C'TryA_G Cuts at: 826 1045 2407 2862 3074 3850 4985 5666 5857 Size: 219 1362 455 212 776 1135 681 191 Cuts at: 5857 826 Size: 1471 Fragments arranged by size: 1471 1362 1135 776 681 455 219 212 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2769 2769 Size: 6502 SfoI GGC'GCC Cuts at: 2390 2463 2390 Size: 73 6429 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6502 SgrAI Cr'CCGG_yG Cuts at: 1700 2385 1700 Size: 685 5817 SmaI CCC'GGG Cuts at: 1872 3829 1872 Size: 1957 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2234 2303 3032 3206 4606 5507 Size: 197 926 291 69 729 174 1400 901 Cuts at: 5507 5769 820 Size: 262 1553 Fragments arranged by size: 1553 1400 926 901 729 291 262 197 174 69 SnaBI TAC'GTA Cuts at: 493 4293 493 Size: 3800 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6502 SphI G_CATG'C Cuts at: 1132 3860 1132 Size: 2728 3774 SspI AAT'ATT Cuts at: 5 52 4426 5 Size: 47 4374 2081 Fragments arranged by size: 4374 2081 47 StuI AGG'CCT Cuts at: 1517 2843 3072 1517 Size: 1326 229 4947 Fragments arranged by size: 4947 1326 229 StyI C'CwwG_G Cuts at: 513 1065 2004 2799 2844 2913 3039 3645 3660 Size: 552 939 795 45 69 126 606 15 Cuts at: 3660 3941 513 Size: 281 3074 Fragments arranged by size: 3074 939 795 606 552 281 126 69 45 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2684 2831 4529 4546 4699 4884 5329 Size: 1255 1104 147 1698 17 153 185 445 Cuts at: 5329 325 Size: 1498 Fragments arranged by size: 1698 1498 1255 1104 445 185 153 147 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2832 4748 6460 Size: 274 80 33 51 1443 855 1916 1712 Cuts at: 6460 96 Size: 138 Fragments arranged by size: 1916 1712 1443 855 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2037 2069 2956 2988 3479 3511 6462 6494 2037 Size: 32 887 32 491 32 2951 32 2045 Fragments arranged by size: 2951 2045 887 491 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6502 XbaI T'CTAG_A Cuts at: 3838 3838 Size: 6502 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2368 2821 2368 Size: 453 6049 XhoI C'TCGA_G Cuts at: 1943 2234 1943 Size: 291 6211 XmaI C'CCGG_G Cuts at: 1870 3827 1870 Size: 1957 4545 XmnI GAAnn'nnTTC Cuts at: 4631 4631 Size: 6502 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4310 276 Size: 53 83 186 936 2776 2468 Fragments arranged by size: 2776 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoRV FalI FspAI NruI NsiI PacI PmlI PsrI RsrII SanDI SapI SexAI SrfI SwaI