Restriction map for
pFN21AB9881
(Linear) (Six-base) MAPSORT of: KIBB9881.seq Check: 1292 from: 1 to: 1797
KIBB9881.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 199 984 1797
Size: 199 785 813
Fragments arranged by size:
813 785 199
Acc65I G'GTAC_C
Cuts at: 0 1667 1797
Size: 1667 130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 1797
Size: 157 1640
AflIII A'CryG_T
Cuts at: 0 569 1797
Size: 569 1228
AlwNI CAG_nnn'CTG
Cuts at: 0 672 819 1797
Size: 672 147 978
Fragments arranged by size:
978 672 147
ApaI G_GGCC'C
Cuts at: 0 7 1431 1797
Size: 7 1424 366
Fragments arranged by size:
1424 366 7
AvaI C'yCGr_G
Cuts at: 0 229 521 918 1797
Size: 229 292 397 879
Fragments arranged by size:
879 397 292 229
BamHI G'GATC_C
Cuts at: 0 1056 1797
Size: 1056 741
BanI G'GyrC_C
Cuts at: 0 383 456 477 958 1471 1614 1667 1797
Size: 383 73 21 481 513 143 53 130
Fragments arranged by size:
513 481 383 143 130 73 53 21
BanII G_rGCy'C
Cuts at: 0 7 1399 1431 1797
Size: 7 1392 32 366
Fragments arranged by size:
1392 366 32 7
BbeI G_GCGC'C
Cuts at: 0 387 460 1797
Size: 387 73 1337
Fragments arranged by size:
1337 387 73
BbsI GAAGACnn'nnnn_
Cuts at: 0 1586 1797
Size: 1586 211
BbvCI CC'TCA_GC
Cuts at: 0 1418 1797
Size: 1418 379
BglI GCCn_nnn'nGGC
Cuts at: 0 157 469 764 1117 1765 1797
Size: 157 312 295 353 648 32
Fragments arranged by size:
648 353 312 295 157 32
BlpI GC'TnA_GC
Cuts at: 0 124 1797
Size: 124 1673
Bme1580I G_kGCm'C
Cuts at: 0 7 482 1431 1476 1495 1797
Size: 7 475 949 45 19 302
Fragments arranged by size:
949 475 302 45 19 7
BmgBI CAC'GTC
Cuts at: 0 570 1797
Size: 570 1227
BmrI ACTGGGnnnn_n'
Cuts at: 0 897 1434 1482 1797
Size: 897 537 48 315
Fragments arranged by size:
897 537 315 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1413 1416 1797
Size: 1413 3 381
Fragments arranged by size:
1413 381 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 994 1797
Size: 994 803
Bpu10I CC'TnA_GC
Cuts at: 0 673 1418 1797
Size: 673 745 379
Fragments arranged by size:
745 673 379
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 1012 1186 1797
Size: 283 729 174 611
Fragments arranged by size:
729 611 283 174
BsaI GGTCTCn'nnnn_
Cuts at: 0 580 1084 1797
Size: 580 504 713
Fragments arranged by size:
713 580 504
BsaHI Gr'CG_yC
Cuts at: 0 160 384 457 1797
Size: 160 224 73 1340
Fragments arranged by size:
1340 224 160 73
BsaWI w'CCGG_w
Cuts at: 0 69 1797
Size: 69 1728
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 157 187 1660 1690 1797
Size: 157 30 1473 30 107
Fragments arranged by size:
1473 157 107 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 340 541 1237 1240 1243 1246 1258
Size: 76 264 201 696 3 3 3 12
Cuts at: 1258 1261 1288 1291 1303 1306 1309 1312 1351
Size: 3 27 3 12 3 3 3 39
Cuts at: 1351 1360 1363 1389 1393 1405 1612 1797
Size: 9 3 26 4 12 207 185
Fragments arranged by size:
696 264 207 201 185 76 39 27
26 12 12 12 9 4 3 3
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 330 665 1074 1624 1797
Size: 330 335 409 550 173
Fragments arranged by size:
550 409 335 330 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1078 1720 1797
Size: 955 123 642 77
Fragments arranged by size:
955 642 123 77
BsiEI CG_ry'CG
Cuts at: 0 473 652 891 1797
Size: 473 179 239 906
Fragments arranged by size:
906 473 239 179
BsiHKAI G_wGCw'C
Cuts at: 0 130 505 1399 1797
Size: 130 375 894 398
Fragments arranged by size:
894 398 375 130
Bsp1286I G_dGCh'C
Cuts at: 0 7 130 482 505 1399 1431 1476 1495
Size: 7 123 352 23 894 32 45 19
Cuts at: 1495 1797
Size: 302
Fragments arranged by size:
894 352 302 123 45 32 23 19
7
BspEI T'CCGG_A
Cuts at: 0 69 1797
Size: 69 1728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 83 199 984 1797
Size: 83 116 785 813
Fragments arranged by size:
813 785 116 83
BsrFI r'CCGG_y
Cuts at: 0 271 279 380 730 763 886 1447 1797
Size: 271 8 101 350 33 123 561 350
Fragments arranged by size:
561 350 350 271 123 101 33 8
BssHII G'CGCG_C
Cuts at: 0 147 425 516 1797
Size: 147 278 91 1281
Fragments arranged by size:
1281 278 147 91
BstYI r'GATC_y
Cuts at: 0 260 1056 1797
Size: 260 796 741
Fragments arranged by size:
796 741 260
Bsu36I CC'TnA_GG
Cuts at: 0 1062 1797
Size: 1062 735
BtgI C'CryG_G
Cuts at: 0 387 473 623 1034 1640 1655 1797
Size: 387 86 150 411 606 15 142
Fragments arranged by size:
606 411 387 150 142 86 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 228 1797
Size: 228 1569
BtsI GCAGTG_nn'
Cuts at: 0 498 1127 1797
Size: 498 629 670
Fragments arranged by size:
670 629 498
DraIII CAC_nnn'GTG
Cuts at: 0 393 1101 1797
Size: 393 708 696
Fragments arranged by size:
708 696 393
DrdI GACnn_nn'nnGTC
Cuts at: 0 653 1797
Size: 653 1144
EaeI y'GGCC_r
Cuts at: 0 470 545 649 728 740 821 884 888
Size: 470 75 104 79 12 81 63 4
Cuts at: 888 1037 1797
Size: 149 760
Fragments arranged by size:
760 470 149 104 81 79 75 63
12 4
EagI C'GGCC_G
Cuts at: 0 470 649 888 1797
Size: 470 179 239 909
Fragments arranged by size:
909 470 239 179
EarI CTCTTCn'nnn_
Cuts at: 0 1214 1235 1247 1265 1328 1370 1541 1556
Size: 1214 21 12 18 63 42 171 15
Cuts at: 1556 1797
Size: 241
Fragments arranged by size:
1214 241 171 63 42 21 18 15
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 367 1797
Size: 367 1430
EcoICRI GAG'CTC
Cuts at: 0 1397 1797
Size: 1397 400
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 994 1797
Size: 157 837 803
Fragments arranged by size:
837 803 157
EcoNI CCTnn'n_nnAGG
Cuts at: 0 79 1797
Size: 79 1718
EcoO109I rG'GnC_Cy
Cuts at: 0 4 816 873 1089 1797
Size: 4 812 57 216 708
Fragments arranged by size:
812 708 216 57 4
FseI GG_CCGG'CC
Cuts at: 0 890 1797
Size: 890 907
FspI TGC'GCA
Cuts at: 0 79 316 1797
Size: 79 237 1481
Fragments arranged by size:
1481 237 79
HaeII r_GCGC'y
Cuts at: 0 102 313 387 460 581 672 1797
Size: 102 211 74 73 121 91 1125
Fragments arranged by size:
1125 211 121 102 91 74 73
KasI G'GCGC_C
Cuts at: 0 383 456 1797
Size: 383 73 1341
Fragments arranged by size:
1341 383 73
KpnI G_GTAC'C
Cuts at: 0 1671 1797
Size: 1671 126
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 380 1797
Size: 143 237 1417
Fragments arranged by size:
1417 237 143
MscI TGG'CCA
Cuts at: 0 823 1797
Size: 823 974
MslI CAynn'nnrTG
Cuts at: 0 850 1797
Size: 850 947
MspA1I CmG'CkG
Cuts at: 0 21 407 475 625 723 939 972 1074
Size: 21 386 68 150 98 216 33 102
Cuts at: 1074 1797
Size: 723
Fragments arranged by size:
723 386 216 150 102 98 68 33
21
NaeI GCC'GGC
Cuts at: 0 273 382 732 765 888 1797
Size: 273 109 350 33 123 909
Fragments arranged by size:
909 350 273 123 109 33
NarI GG'CG_CC
Cuts at: 0 384 457 1797
Size: 384 73 1340
Fragments arranged by size:
1340 384 73
NcoI C'CATG_G
Cuts at: 0 1034 1640 1655 1797
Size: 1034 606 15 142
Fragments arranged by size:
1034 606 142 15
NgoMIV G'CCGG_C
Cuts at: 0 271 380 730 763 886 1797
Size: 271 109 350 33 123 911
Fragments arranged by size:
911 350 271 123 109 33
NspI r_CATG'y
Cuts at: 0 934 1797
Size: 934 863
PasI CC'CwG_GG
Cuts at: 0 1431 1797
Size: 1431 366
PflMI CCAn_nnn'nTGG
Cuts at: 0 57 362 393 596 819 909 1797
Size: 57 305 31 203 223 90 888
Fragments arranged by size:
888 305 223 203 90 57 31
PfoI T'CCnGG_A
Cuts at: 0 29 50 1508 1797
Size: 29 21 1458 289
Fragments arranged by size:
1458 289 29 21
PpuMI rG'GwC_Cy
Cuts at: 0 873 1797
Size: 873 924
PspOMI G'GGCC_C
Cuts at: 0 3 1427 1797
Size: 3 1424 370
Fragments arranged by size:
1424 370 3
PspXI vC'TCGA_Gb
Cuts at: 0 229 1797
Size: 229 1568
PstI C_TGCA'G
Cuts at: 0 406 861 1073 1797
Size: 406 455 212 724
Fragments arranged by size:
724 455 406 212
PvuII CAG'CTG
Cuts at: 0 21 939 1074 1797
Size: 21 918 135 723
Fragments arranged by size:
918 723 135 21
SacI G_AGCT'C
Cuts at: 0 1399 1797
Size: 1399 398
SacII CC_GC'GG
Cuts at: 0 476 626 1797
Size: 476 150 1171
Fragments arranged by size:
1171 476 150
ScaI AGT'ACT
Cuts at: 0 829 1797
Size: 829 968
SfcI C'TryA_G
Cuts at: 0 402 857 1069 1797
Size: 402 455 212 728
Fragments arranged by size:
728 455 402 212
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 764 1797
Size: 764 1033
SfoI GGC'GCC
Cuts at: 0 385 458 1797
Size: 385 73 1339
Fragments arranged by size:
1339 385 73
SgrAI Cr'CCGG_yG
Cuts at: 0 380 1797
Size: 380 1417
SmlI C'TyrA_G
Cuts at: 0 229 298 1027 1201 1797
Size: 229 69 729 174 596
Fragments arranged by size:
729 596 229 174 69
StuI AGG'CCT
Cuts at: 0 838 1067 1797
Size: 838 229 730
Fragments arranged by size:
838 730 229
StyI C'CwwG_G
Cuts at: 0 794 839 908 1034 1640 1655 1797
Size: 794 45 69 126 606 15 142
Fragments arranged by size:
794 606 142 126 69 45 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 679 826 1797
Size: 679 147 971
Fragments arranged by size:
971 679 147
TatI w'GTAC_w
Cuts at: 0 827 1797
Size: 827 970
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 32 64 951 983 1474 1506 1797
Size: 32 32 887 32 491 32 291
Fragments arranged by size:
887 491 291 32 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 363 816 1797
Size: 363 453 981
Fragments arranged by size:
981 453 363
XhoI C'TCGA_G
Cuts at: 0 229 1797
Size: 229 1568
Enzymes that do cut:
AarI Acc65I AcuI AflIII AlwNI ApaI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspMI BsrFI BssHII BstYI Bsu36I BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FseI FspI HaeII KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PflMI PfoI PpuMI PspOMI PspXI PstI
PvuII SacI SacII ScaI SfcI SfiI SfoI SgrAI
SmlI StuI StyI TaqII TatI TstI XcmI XhoI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BcgI BciVI BclI BfrBI BglII BmtI BsaAI
BsaBI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstZ17I ClaI CspCI
DraI EcoRI EcoRV FalI FspAI Hin4I HincII HindIII
HpaI MfeI MluI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PsrI
PvuI RsrII SalI SanDI SapI SbfI SexAI SgfI
SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XmaI XmnI ZraI