Restriction map for
pFN21AB8875
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI HphI
KasI Ksp632I KspI MboII MfeI
MflI MluI MluNI MroI MscI
MseI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI ScaI SexAI
SfiI SfoI SfuI SmlI SnaBI
SpeI SphI SspBI SspI StuI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 220 GGTACC G^GTACC
AfaI 221 GTAC GT^AC
Asp718I 220 GGTACC G^GTACC
AspHI 283 GAGCAC G[AT]GC[AT]^C
AvaI 50 CCCGGG C^[CT]CG[GA]G
BanII 449 GGGCTC G[GA]GC[CT]^C
BcgI 158 GCACCGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 277 CTAG C^TAG
BsaBI 391 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 415 GGTCTC GGTCTC(1/5)
BsaWI 217 TCCGGT [AT]^CCGG[AT]
BseRI 10 GAGGAG GAGGAG(10/8)
BsiEI 27 CGACCG CG[GA][CT]^CG
BsiHKAI 283 GAGCAC G[AT]GC[AT]^C
BsmAI 416 GTCTC GTCTC(1/5)
BsmFI 404 GGGAC GGGAC(10/14)
BsoBI 50 CCCGGG C^[CT]CG[GA]G
BspHI 143 TCATGA T^CATGA
BspMI 138 GCAGGT ACCTGC(4/8)
BssSI 460 CTCGTG CACGAG(-5/-1)
BstAPI 63 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 164 CCATCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 391 GATC ^GATC
DraIII 481 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I 425 CCTTGG C^C[AT][AT]GG
HaeII 132 GGCGCT [GA]GCGC^[CT]
HincII 298 GTCAAC GT[CT]^[GA]AC
HindII 298 GTCAAC GT[CT]^[GA]AC
KpnI 220 GGTACC GGTAC^C
MaeI 277 CTAG C^TAG
MaeIII 353 GTCAC ^GT[ACGT]AC
MamI 391 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 391 GATC ^GATC
MslI 357 CACTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 22 GCCGGC GCC^GGC
NdeII 391 GATC ^GATC
NgoMIV 22 GCCGGC G^CCGGC
PflMI 480 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 380 CAGCTG CAG^CTG
RcaI 143 TCATGA T^CATGA
RsaI 221 GTAC GT^AC
Sau3AI 391 GATC ^GATC
SbfI 471 CCTGCAGG CCTGCA^GG
SfaNI 99 GCATC GCATC(5/9)
SgrAI 21 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 50 CCCGGG CCC^GGG
Sse8387I 471 CCTGCAGG CCTGCA^GG
StyI 425 CCTTGG C^C[AT][AT]GG
Tsp45I 353 GTCAC ^GT[GC]AC
Van91I 480 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 50 CCCGGG C^CCGGG
XmaI 50 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 19 CGCG CG^CG
88 CGCG
AcyI 106 GACGCC G[GA]^CG[CT]C
406 GACGCC
AvaII 273 GGTCC G^G[AT]CC
456 GGACC
BpmI 7 CTGGAG CTGGAG(16/14)
172 CTGGAG
BsaHI 106 GACGCC G[GA]^CG[CT]C
406 GACGCC
BsgI 82 CTGCAC GTGCAG(16/14)
61 GTGCAG
BsrFI 22 GCCGGC [GA]^CCGG[CT]
29 ACCGGC
BstF5I 100 CATCC GGATG(2/0)
468 CATCC
BstUI 19 CGCG CG^CG
88 CGCG
Cfr10I 22 GCCGGC [GA]^CCGG[CT]
29 ACCGGC
DdeI 34 CTGAG C^T[ACGT]AG
452 CTCAG
DraII 272 AGGTCCT [GA]G^G[ACGT]CC[CT]
455 AGGACCT
EcoO109I 272 AGGTCCT [GA]G^G[ACGT]CC[CT]
455 AGGACCT
FauI 245 CCCGC CCCGC(4/6)
492 CCCGC
FokI 100 CATCC GGATG(9/13)
468 CATCC
Hin1I 106 GACGCC G[GA]^CG[CT]C
406 GACGCC
HinfI 254 GATTC G^A[ACGT]TC
385 GAATC
MaeII 59 ACGT A^CGT
302 ACGT
MspA1I 380 CAGCTG C[AC]G^C[GT]G
493 CCGCTG
MvnI 19 CGCG CG^CG
88 CGCG
PpuMI 272 AGGTCCT [GA]G^G[AT]CC[CT]
455 AGGACCT
TfiI 254 GATTC G^A[AT]TC
385 GAATC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI BanI BbvI BcnI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstNI Cac8I CfoI
Cfr13I EcoRII Fnu4HI HaeIII HapII
HgaI HhaI HinP1I HpaII ItaI
MnlI MspI MvaI MwoI NciI
NlaIII NlaIV PstI Sau96I ScrFI
SfcI TseI TspRI