(Circular) (Six-base) MAPSORT of: pFN21AA1633.seq Check: 4796 from: 1 to: 10147 pFN21AA1633 10147 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2405 2458 2405 Size: 53 10094 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2034 7957 278 Size: 53 83 186 936 498 5923 2468 Fragments arranged by size: 5923 2468 936 498 186 83 53 AccI GT'mk_AC Cuts at: 1937 4007 4094 7490 1937 Size: 2070 87 3396 4594 Fragments arranged by size: 4594 3396 2070 87 Acc65I G'GTAC_C Cuts at: 7468 7468 Size: 10147 AclI AA'CG_TT Cuts at: 1563 5018 8274 8647 1563 Size: 3455 3256 373 3063 Fragments arranged by size: 3455 3256 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2363 2430 3123 3467 4046 4571 4949 5001 Size: 731 67 693 344 579 525 378 52 Cuts at: 5001 5217 5232 6048 6138 6470 6644 6707 7178 Size: 216 15 816 90 332 174 63 471 Cuts at: 7178 8210 9594 9862 1632 Size: 1032 1384 268 1917 Fragments arranged by size: 1917 1384 1032 816 731 693 579 525 471 378 344 332 268 216 174 90 67 63 52 15 AflII C'TTAA_G Cuts at: 820 1017 3962 4249 820 Size: 197 2945 287 6718 Fragments arranged by size: 6718 2945 287 197 AflIII A'CryG_T Cuts at: 1203 3474 5548 9046 10023 1203 Size: 2271 2074 3498 977 1327 Fragments arranged by size: 3498 2271 2074 1327 977 AgeI A'CCGG_T Cuts at: 8978 8978 Size: 10147 AhdI GACnn_n'nnGTC Cuts at: 8876 8876 Size: 10147 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 10147 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4318 1184 Size: 3134 7013 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7060 7092 7411 7443 7060 Size: 32 319 32 9764 Fragments arranged by size: 9764 319 32 32 AlwNI CAG_nnn'CTG Cuts at: 2058 3226 4024 5620 6568 6601 6640 7890 9462 Size: 1168 798 1596 948 33 39 1250 1572 Cuts at: 9462 2058 Size: 2743 Fragments arranged by size: 2743 1596 1572 1250 1168 948 798 39 33 ApaI G_GGCC'C Cuts at: 4291 5464 4291 Size: 1173 8974 ApaLI G'TGCA_C Cuts at: 8204 9360 8204 Size: 1156 8991 ApoI r'AATT_y Cuts at: 1493 2276 3089 3488 3811 3846 5386 5656 6207 Size: 783 813 399 323 35 1540 270 551 Cuts at: 6207 6290 6357 7456 7682 7834 9943 1493 Size: 83 67 1099 226 152 2109 1697 Fragments arranged by size: 2109 1697 1540 1099 813 783 551 399 323 270 226 152 83 67 35 AseI AT'TA_AT Cuts at: 160 1032 4167 5313 8701 160 Size: 872 3135 1146 3388 1606 Fragments arranged by size: 3388 3135 1606 1146 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10147 AvaI C'yCGr_G Cuts at: 1870 1943 7184 7472 1870 Size: 73 5241 288 4545 Fragments arranged by size: 5241 4545 288 73 BamHI G'GATC_C Cuts at: 2877 6410 7477 7844 2877 Size: 3533 1067 367 5180 Fragments arranged by size: 5180 3533 1067 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4747 7468 8923 618 Size: 325 217 627 2960 2721 1455 1842 Fragments arranged by size: 2960 2721 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2322 2781 3558 4291 5228 5464 5763 7411 Size: 931 459 777 733 937 236 299 1648 Cuts at: 7411 7466 1391 Size: 55 4072 Fragments arranged by size: 4072 1648 937 931 777 733 459 299 236 55 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3111 3386 3827 4484 6024 6155 6470 Size: 971 1212 275 441 657 1540 131 315 Cuts at: 6470 928 Size: 4605 Fragments arranged by size: 4605 1540 1212 971 657 441 315 275 131 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8338 8372 1903 Size: 34 6401 34 3678 Fragments arranged by size: 6401 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4238 8039 9249 4238 Size: 3801 1210 5136 Fragments arranged by size: 5136 3801 1210 BclI T'GATC_A Cuts at: 1548 3438 3886 5128 5296 7339 1548 Size: 1890 448 1242 168 2043 4356 Fragments arranged by size: 4356 2043 1890 1242 448 168 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2528 3062 3143 Size: 107 122 71 631 605 856 534 81 Cuts at: 3143 4756 8758 136 Size: 1613 4002 1525 Fragments arranged by size: 4002 1613 1525 856 631 605 534 122 107 81 71 BglII A'GATC_T Cuts at: 2476 4905 5710 10142 2476 Size: 2429 805 4432 2481 Fragments arranged by size: 4432 2481 2429 805 BlpI GC'TnA_GC Cuts at: 2051 2404 5031 5922 6602 7310 7564 2051 Size: 353 2627 891 680 708 254 4634 Fragments arranged by size: 4634 2627 891 708 680 353 254 Bme1580I G_kGCm'C Cuts at: 1790 4291 4596 5464 7202 8208 9364 1790 Size: 2501 305 868 1738 1006 1156 2573 Fragments arranged by size: 2573 2501 1738 1156 1006 868 305 BmgBI CAC'GTC Cuts at: 1318 3475 1318 Size: 2157 7990 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2070 5410 7878 Size: 416 53 83 399 24 653 3340 2468 Cuts at: 7878 8836 442 Size: 958 1753 Fragments arranged by size: 3340 2468 1753 958 653 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4122 1056 Size: 3066 7081 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5170 6470 6472 7196 5170 Size: 1300 2 724 8121 Fragments arranged by size: 8121 1300 724 2 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2339 2793 3278 3290 3806 4470 5125 5621 Size: 1100 454 485 12 516 664 655 496 Cuts at: 5621 5642 5951 6442 6497 7189 7409 8807 1239 Size: 21 309 491 55 692 220 1398 2579 Fragments arranged by size: 2579 1398 1100 692 664 655 516 496 491 485 454 309 220 55 21 12 Bpu10I CC'TnA_GC Cuts at: 3557 4485 6057 7394 3557 Size: 928 1572 1337 6310 Fragments arranged by size: 6310 1572 1337 928 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2276 2306 3572 3936 5494 6162 6388 8272 9137 Size: 30 1266 364 1558 668 226 1884 865 Cuts at: 9137 9435 2276 Size: 298 2988 Fragments arranged by size: 2988 1884 1558 1266 865 668 364 298 226 30 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6114 8810 882 Size: 617 4615 2696 2219 Fragments arranged by size: 4615 2696 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5542 5551 7938 493 Size: 711 4338 9 2387 2702 Fragments arranged by size: 4338 2702 2387 711 9 BsaBI GATnn'nnATC Cuts at: 10141 10141 Size: 10147 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2031 7954 8336 8971 Size: 53 83 186 936 498 5923 382 635 Cuts at: 8971 275 Size: 1451 Fragments arranged by size: 5923 1451 936 635 498 382 186 83 53 BsaWI w'CCGG_w Cuts at: 8580 8978 9252 9399 9810 8580 Size: 398 274 147 411 8917 Fragments arranged by size: 8917 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2146 2176 6611 6641 6830 Size: 30 165 30 743 30 4435 30 189 Cuts at: 6830 6836 6860 6866 6868 6898 6990 7020 1178 Size: 6 24 6 2 30 92 30 4305 Fragments arranged by size: 4435 4305 743 189 165 92 30 30 30 30 30 30 24 6 6 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2690 2744 3845 5864 6632 6810 1186 Size: 1504 54 1101 2019 768 178 4523 Fragments arranged by size: 4523 2019 1504 1101 768 178 54 BseYI C'CCAG_C Cuts at: 2114 4569 7043 7063 7084 7430 9350 9846 2114 Size: 2455 2474 20 21 346 1920 496 2415 Fragments arranged by size: 2474 2455 2415 1920 496 346 21 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2483 2817 3289 4643 4692 4724 5440 Size: 573 747 334 472 1354 49 32 716 Cuts at: 5440 6632 1163 Size: 1192 4678 Fragments arranged by size: 4678 1354 1192 747 716 573 472 334 49 32 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7597 8358 9386 1540 Size: 275 186 5596 761 1028 2301 Fragments arranged by size: 5596 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2176 2322 2781 3558 3590 5763 6483 6717 7121 Size: 146 459 777 32 2173 720 234 404 Cuts at: 7121 8208 8293 9364 2176 Size: 1087 85 1071 2959 Fragments arranged by size: 2959 2173 1087 1071 777 720 459 404 234 146 85 32 BsmI GAATG_Cn' Cuts at: 2271 4319 5032 7658 7751 2271 Size: 2048 713 2626 93 4667 Fragments arranged by size: 4667 2626 2048 713 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2555 4868 2555 Size: 2313 7834 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2176 2322 2781 3558 3590 4291 4596 Size: 399 386 146 459 777 32 701 305 Cuts at: 4596 5228 5464 5763 6483 6717 7121 7202 7411 Size: 632 236 299 720 234 404 81 209 Cuts at: 7411 7466 8208 8293 9364 1391 Size: 55 742 85 1071 2174 Fragments arranged by size: 2174 1071 777 742 720 701 632 459 404 399 386 305 299 236 234 209 146 85 81 55 32 BspEI T'CCGG_A Cuts at: 9810 9810 Size: 10147 BspHI T'CATG_A Cuts at: 8036 9766 8036 Size: 1730 8417 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2405 2458 3750 4338 4656 4746 7502 844 Size: 1561 53 1292 588 318 90 2756 3489 Fragments arranged by size: 3489 2756 1561 1292 588 318 90 53 BsrBI CCG'CTC Cuts at: 1394 1823 8034 1394 Size: 429 6211 3507 Fragments arranged by size: 6211 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3352 3378 6402 8642 8816 65 Size: 1183 2104 26 3024 2240 174 1396 Fragments arranged by size: 3024 2240 2104 1396 1183 174 26 BsrFI r'CCGG_y Cuts at: 1700 1810 8791 8978 1700 Size: 110 6981 187 2869 Fragments arranged by size: 6981 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4504 96 Size: 4408 5739 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10147 BssSI C'ACGA_G Cuts at: 2621 6117 8207 9219 2621 Size: 3496 2090 1012 3549 Fragments arranged by size: 3549 3496 2090 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3796 4747 5533 6640 794 Size: 3002 951 786 1107 4301 Fragments arranged by size: 4301 3002 1107 951 786 BstBI TT'CG_AA Cuts at: 6211 8951 6211 Size: 2740 7407 BstEII G'GTnAC_C Cuts at: 1184 7240 1184 Size: 6056 4091 BstXI CCAn_nnnn'nTGG Cuts at: 4070 4298 5872 4070 Size: 228 1574 8345 Fragments arranged by size: 8345 1574 228 BstYI r'GATC_y Cuts at: 1972 2476 2877 3547 4905 5682 5710 6284 6410 Size: 504 401 670 1358 777 28 574 126 Cuts at: 6410 7477 7844 8229 8246 9698 9784 9796 10142 Size: 1067 367 385 17 1452 86 12 346 Cuts at: 10142 1972 Size: 1977 Fragments arranged by size: 1977 1452 1358 1067 777 670 574 504 401 385 367 346 126 86 28 17 12 BstZ17I GTA'TAC Cuts at: 4008 4008 Size: 10147 BtgI C'CryG_G Cuts at: 513 1065 2976 4635 5794 6747 513 Size: 552 1911 1659 1159 953 3913 Fragments arranged by size: 3913 1911 1659 1159 953 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2651 487 Size: 683 56 1425 7983 Fragments arranged by size: 7983 1425 683 56 BtsI GCAGTG_nn' Cuts at: 2068 4704 4992 5428 6798 7117 7663 8457 8477 Size: 2636 288 436 1370 319 546 794 20 Cuts at: 8477 2068 Size: 3738 Fragments arranged by size: 3738 2636 1370 794 546 436 319 288 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10147 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10112 DraI TTT'AAA Cuts at: 4939 7451 4939 Size: 2512 7635 DraIII CAC_nnn'GTG Cuts at: 4747 5533 6888 4747 Size: 786 1355 8006 Fragments arranged by size: 8006 1355 786 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2036 5299 9154 809 Size: 507 720 3263 3855 1802 Fragments arranged by size: 3855 3263 1802 720 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4632 4727 5791 Size: 54 1425 50 275 18 2802 95 1064 Cuts at: 5791 5886 6592 6910 6937 7122 7192 7594 8483 Size: 95 706 318 27 185 70 402 889 Cuts at: 8483 10112 8 Size: 1629 43 Fragments arranged by size: 2802 1629 1425 1064 889 706 402 318 275 185 95 95 70 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 7594 1537 Size: 275 5782 4090 Fragments arranged by size: 5782 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2019 2580 2690 2886 3284 3595 3651 4683 Size: 666 561 110 196 398 311 56 1032 Cuts at: 4683 6705 7134 8077 1353 Size: 2022 429 943 3423 Fragments arranged by size: 3423 2022 1032 943 666 561 429 398 311 196 110 56 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8734 9108 9254 387 Size: 1064 138 7145 374 146 1280 Fragments arranged by size: 7145 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2320 2779 3556 5761 2320 Size: 459 777 2205 6706 Fragments arranged by size: 6706 2205 777 459 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2339 2363 2430 2793 3123 3278 3290 Size: 393 707 24 67 363 330 155 12 Cuts at: 3290 3467 3806 4046 4470 4571 4949 5001 5125 Size: 177 339 240 424 101 378 52 124 Cuts at: 5125 5217 5232 5621 5642 5951 6048 6138 6442 Size: 92 15 389 21 309 97 90 304 Cuts at: 6442 6470 6497 6644 6707 7178 7189 7409 8210 Size: 28 27 147 63 471 11 220 801 Cuts at: 8210 8807 9594 9862 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 801 787 707 597 471 424 393 389 378 363 339 330 309 304 268 240 220 177 155 147 124 101 97 92 90 67 63 52 28 27 24 21 15 12 11 EcoNI CCTnn'n_nnAGG Cuts at: 2726 6959 7139 7370 2726 Size: 4233 180 231 5503 Fragments arranged by size: 5503 4233 231 180 EcoO109I rG'GnC_Cy Cuts at: 2896 3064 3265 4287 5460 6385 6973 2896 Size: 168 201 1022 1173 925 588 6070 Fragments arranged by size: 6070 1173 1022 925 588 201 168 EcoRI G'AATT_C Cuts at: 7456 7834 7456 Size: 378 9769 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3977 4517 4698 4831 6127 3977 Size: 540 181 133 1296 7997 Fragments arranged by size: 7997 1296 540 181 133 FspI TGC'GCA Cuts at: 8653 8653 Size: 10147 HaeII r_GCGC'y Cuts at: 1678 7321 9294 1678 Size: 5643 1973 2531 Fragments arranged by size: 5643 2531 1973 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2720 2734 2750 2752 2766 Size: 32 135 32 1148 14 16 2 14 Cuts at: 2766 2782 3430 3462 3878 3910 5674 5706 6830 Size: 16 648 32 416 32 1764 32 1124 Cuts at: 6830 6836 6862 6868 6990 7022 8788 8820 8862 Size: 6 26 6 122 32 1766 32 42 Cuts at: 8862 8894 1373 Size: 32 2626 Fragments arranged by size: 2626 1766 1764 1148 1124 648 416 135 122 42 32 32 32 32 32 32 32 32 26 16 16 14 14 6 6 2 HincII GTy'rAC Cuts at: 669 1657 1938 5819 7491 7737 669 Size: 988 281 3881 1672 246 3079 Fragments arranged by size: 3881 3079 1672 988 281 246 HindIII A'AGCT_T Cuts at: 748 2597 2706 7160 7298 748 Size: 1849 109 4454 138 3597 Fragments arranged by size: 4454 3597 1849 138 109 HpaI GTT'AAC Cuts at: 7737 7737 Size: 10147 KpnI G_GTAC'C Cuts at: 7472 7472 Size: 10147 MfeI C'AATT_G Cuts at: 7343 7746 7343 Size: 403 9744 MluI A'CGCG_T Cuts at: 10023 10023 Size: 10147 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2001 5950 6252 9261 9445 1305 Size: 696 3949 302 3009 184 2007 Fragments arranged by size: 3949 3009 2007 696 302 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4634 5793 6912 7194 10 Size: 54 1425 343 2802 1159 1119 282 2963 Fragments arranged by size: 2963 2802 1425 1159 1119 343 282 54 MslI CAynn'nnrTG Cuts at: 518 1166 4461 8105 8464 8623 518 Size: 648 3295 3644 359 159 2042 Fragments arranged by size: 3644 3295 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2055 2371 2434 4894 5569 5620 5908 6490 Size: 449 316 63 2460 675 51 288 582 Cuts at: 6490 6568 6585 6591 6640 7309 7563 8240 9388 Size: 78 17 6 49 669 254 677 1148 Cuts at: 9388 9633 1606 Size: 245 2120 Fragments arranged by size: 2460 2120 1148 677 675 669 582 449 316 288 254 245 78 63 51 49 17 6 NcoI C'CATG_G Cuts at: 513 1065 2976 5794 6747 513 Size: 552 1911 2818 953 3913 Fragments arranged by size: 3913 2818 1911 953 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 10147 NheI G'CTAG_C Cuts at: 1052 4118 1052 Size: 3066 7081 NotI GC'GGCC_GC Cuts at: 7594 7594 Size: 10147 NspI r_CATG'y Cuts at: 1132 1229 2751 5668 7425 7505 9050 1132 Size: 97 1522 2917 1757 80 1545 2229 Fragments arranged by size: 2917 2229 1757 1545 1522 97 80 PasI CC'CwG_GG Cuts at: 1348 2042 1348 Size: 694 9453 PciI A'CATG_T Cuts at: 9046 9046 Size: 10147 PflMI CCAn_nnn'nTGG Cuts at: 1108 4882 4909 5359 6830 6949 1108 Size: 3774 27 450 1471 119 4306 Fragments arranged by size: 4306 3774 1471 450 119 27 PfoI T'CCnGG_A Cuts at: 2355 2649 5898 6458 6573 7414 2355 Size: 294 3249 560 115 841 5088 Fragments arranged by size: 5088 3249 841 560 294 115 PmeI GTTT'AAAC Cuts at: 7451 7451 Size: 10147 PmlI CAC'GTG Cuts at: 5551 5551 Size: 10147 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8216 8248 9729 9761 8216 Size: 32 1481 32 8602 Fragments arranged by size: 8602 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2896 3265 2896 Size: 369 9778 PshAI GACnn'nnGTC Cuts at: 1613 4090 1613 Size: 2477 7670 PsiI TTA'TAA Cuts at: 7717 7717 Size: 10147 PspOMI G'GGCC_C Cuts at: 4287 5460 4287 Size: 1173 8974 PstI C_TGCA'G Cuts at: 830 2063 3544 3720 3747 4899 4920 5646 5697 Size: 1233 1481 176 27 1152 21 726 51 Cuts at: 5697 5862 6531 6590 7499 830 Size: 165 669 59 909 3478 Fragments arranged by size: 3478 1481 1233 1152 909 726 669 176 165 59 51 27 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10147 PvuII CAG'CTG Cuts at: 2371 4894 5620 5908 6568 6640 7309 2371 Size: 2523 726 288 660 72 669 5209 Fragments arranged by size: 5209 2523 726 669 660 288 72 RsrII CG'GwC_CG Cuts at: 5564 5564 Size: 10147 SacI G_AGCT'C Cuts at: 2322 2781 3558 5763 2322 Size: 459 777 2205 6706 Fragments arranged by size: 6706 2205 777 459 SalI G'TCGA_C Cuts at: 1936 7489 1936 Size: 5553 4594 SapI GCTCTTCn'nnn_ Cuts at: 2580 2690 3284 3595 3651 6705 2580 Size: 110 594 311 56 3054 6022 Fragments arranged by size: 6022 3054 594 311 110 56 SbfI CC_TGCA'GG Cuts at: 3747 7499 3747 Size: 3752 6395 ScaI AGT'ACT Cuts at: 8395 10107 8395 Size: 1712 8435 SfcI C'TryA_G Cuts at: 826 1045 2059 2925 3068 3540 3716 3743 4095 Size: 219 1014 866 143 472 176 27 352 Cuts at: 4095 4895 4916 5642 5693 5858 6527 6586 7147 Size: 800 21 726 51 165 669 59 561 Cuts at: 7147 7495 8630 9311 9502 826 Size: 348 1135 681 191 1471 Fragments arranged by size: 1471 1135 1014 866 800 726 681 669 561 472 352 348 219 191 176 165 143 59 51 27 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10147 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10147 SmaI CCC'GGG Cuts at: 1872 7474 1872 Size: 5602 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2255 2321 3551 3951 3962 4249 Size: 197 926 312 66 1230 400 11 287 Cuts at: 4249 5473 6177 6367 8251 9152 9414 820 Size: 1224 704 190 1884 901 262 1553 Fragments arranged by size: 1884 1553 1230 1224 926 901 704 400 312 287 262 197 190 66 11 SnaBI TAC'GTA Cuts at: 493 7938 493 Size: 7445 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10147 SphI G_CATG'C Cuts at: 1132 7505 1132 Size: 6373 3774 SspI AAT'ATT Cuts at: 5 52 8071 5 Size: 47 8019 2081 Fragments arranged by size: 8019 2081 47 StuI AGG'CCT Cuts at: 1517 2546 7145 7399 1517 Size: 1029 4599 254 4265 Fragments arranged by size: 4599 4265 1029 254 StyI C'CwwG_G Cuts at: 513 1065 2801 2976 3825 4596 5794 6747 7586 Size: 552 1736 175 849 771 1198 953 839 Cuts at: 7586 513 Size: 3074 Fragments arranged by size: 3074 1736 1198 953 849 839 771 552 175 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3233 4743 4889 5552 6002 7098 8174 Size: 1255 1653 1510 146 663 450 1096 1076 Cuts at: 8174 8191 8344 8529 8974 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1653 1510 1498 1255 1096 1076 663 450 445 185 153 146 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3176 4504 8393 Size: 274 80 33 51 1443 1199 1328 3889 Cuts at: 8393 10105 96 Size: 1712 138 Fragments arranged by size: 3889 1712 1443 1328 1199 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7181 7213 10107 10139 7181 Size: 32 2894 32 7189 Fragments arranged by size: 7189 2894 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 10147 XbaI T'CTAG_A Cuts at: 7483 7483 Size: 10147 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2116 4366 4879 7132 7384 2011 Size: 105 2250 513 2253 252 4774 Fragments arranged by size: 4774 2253 2250 513 252 105 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10147 XmaI C'CCGG_G Cuts at: 1870 7472 1870 Size: 5602 4545 XmnI GAAnn'nnTTC Cuts at: 4410 5217 5702 6294 8276 4410 Size: 807 485 592 1982 6281 Fragments arranged by size: 6281 1982 807 592 485 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2032 7955 276 Size: 53 83 186 936 498 5923 2468 Fragments arranged by size: 5923 2468 936 498 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI SacII SanDI SexAI SfiI SfoI SrfI SwaI