Restriction map for
pFN21AA1633
(Linear) (Six-base) MAPSORT of: KIAA1633.seq Check: 5148 from: 1 to: 5442
KIAA1633.orf
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 400 453 5442
Size: 400 53 4989
Fragments arranged by size:
4989 400 53
AatII G_ACGT'C
Cuts at: 0 29 5442
Size: 29 5413
AccI GT'mk_AC
Cuts at: 0 2002 2089 5442
Size: 2002 87 3353
Fragments arranged by size:
3353 2002 87
AclI AA'CG_TT
Cuts at: 0 3013 5442
Size: 3013 2429
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 425 1118 1462 2041 2566 2944 2996
Size: 358 67 693 344 579 525 378 52
Cuts at: 2996 3212 3227 4043 4133 4465 4639 4702 5173
Size: 216 15 816 90 332 174 63 471
Cuts at: 5173 5442
Size: 269
Fragments arranged by size:
816 693 579 525 471 378 358 344
332 269 216 174 90 67 63 52
15
AflII C'TTAA_G
Cuts at: 0 1957 2244 5442
Size: 1957 287 3198
Fragments arranged by size:
3198 1957 287
AflIII A'CryG_T
Cuts at: 0 1469 3543 5442
Size: 1469 2074 1899
Fragments arranged by size:
2074 1899 1469
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2313 5442
Size: 2313 3129
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5055 5087 5406 5438 5442
Size: 5055 32 319 32 4
Fragments arranged by size:
5055 319 32 32 4
AlwNI CAG_nnn'CTG
Cuts at: 0 53 1221 2019 3615 4563 4596 4635 5442
Size: 53 1168 798 1596 948 33 39 807
Fragments arranged by size:
1596 1168 948 807 798 53 39 33
ApaI G_GGCC'C
Cuts at: 0 2286 3459 5442
Size: 2286 1173 1983
Fragments arranged by size:
2286 1983 1173
ApoI r'AATT_y
Cuts at: 0 271 1084 1483 1806 1841 3381 3651 4202
Size: 271 813 399 323 35 1540 270 551
Cuts at: 4202 4285 4352 5442
Size: 83 67 1090
Fragments arranged by size:
1540 1090 813 551 399 323 271 270
83 67 35
AseI AT'TA_AT
Cuts at: 0 2162 3308 5442
Size: 2162 1146 2134
Fragments arranged by size:
2162 2134 1146
AvaI C'yCGr_G
Cuts at: 0 5179 5442
Size: 5179 263
BamHI G'GATC_C
Cuts at: 0 872 4405 5442
Size: 872 3533 1037
Fragments arranged by size:
3533 1037 872
BanI G'GyrC_C
Cuts at: 0 2742 5442
Size: 2742 2700
BanII G_rGCy'C
Cuts at: 0 317 776 1553 2286 3223 3459 3758 5406
Size: 317 459 777 733 937 236 299 1648
Cuts at: 5406 5442
Size: 36
Fragments arranged by size:
1648 937 777 733 459 317 299 236
36
BbsI GAAGACnn'nnnn_
Cuts at: 0 1106 1381 1822 2479 4019 4150 4465 5442
Size: 1106 275 441 657 1540 131 315 977
Fragments arranged by size:
1540 1106 977 657 441 315 275 131
BciVI GTATCCnnnnn_n'
Cuts at: 0 2233 5442
Size: 2233 3209
BclI T'GATC_A
Cuts at: 0 1433 1881 3123 3291 5334 5442
Size: 1433 448 1242 168 2043 108
Fragments arranged by size:
2043 1433 1242 448 168 108
BglI GCCn_nnn'nGGC
Cuts at: 0 523 1057 1138 2751 5442
Size: 523 534 81 1613 2691
Fragments arranged by size:
2691 1613 534 523 81
BglII A'GATC_T
Cuts at: 0 471 2900 3705 5442
Size: 471 2429 805 1737
Fragments arranged by size:
2429 1737 805 471
BlpI GC'TnA_GC
Cuts at: 0 46 399 3026 3917 4597 5305 5442
Size: 46 353 2627 891 680 708 137
Fragments arranged by size:
2627 891 708 680 353 137 46
Bme1580I G_kGCm'C
Cuts at: 0 2286 2591 3459 5197 5442
Size: 2286 305 868 1738 245
Fragments arranged by size:
2286 1738 868 305 245
BmgBI CAC'GTC
Cuts at: 0 1470 5442
Size: 1470 3972
BmrI ACTGGGnnnn_n'
Cuts at: 0 65 3405 5442
Size: 65 3340 2037
Fragments arranged by size:
3340 2037 65
BmtI G_CTAG'C
Cuts at: 0 2117 5442
Size: 2117 3325
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3165 4465 4467 5191 5442
Size: 3165 1300 2 724 251
Fragments arranged by size:
3165 1300 724 251 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 788 1273 1285 1801 2465 3120 3616
Size: 334 454 485 12 516 664 655 496
Cuts at: 3616 3637 3946 4437 4492 5184 5404 5442
Size: 21 309 491 55 692 220 38
Fragments arranged by size:
692 664 655 516 496 491 485 454
334 309 220 55 38 21 12
Bpu10I CC'TnA_GC
Cuts at: 0 1552 2480 4052 5389 5442
Size: 1552 928 1572 1337 53
Fragments arranged by size:
1572 1552 1337 928 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 301 1567 1931 3489 4157 4383 5442
Size: 271 30 1266 364 1558 668 226 1059
Fragments arranged by size:
1558 1266 1059 668 364 271 226 30
BsaI GGTCTCn'nnnn_
Cuts at: 0 4109 5442
Size: 4109 1333
BsaAI yAC'GTr
Cuts at: 0 3537 3546 5442
Size: 3537 9 1896
Fragments arranged by size:
3537 1896 9
BsaHI Gr'CG_yC
Cuts at: 0 26 5442
Size: 26 5416
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 141 171 4606 4636 4825 4831 4855 4861
Size: 141 30 4435 30 189 6 24 6
Cuts at: 4861 4863 4893 4985 5015 5442
Size: 2 30 92 30 427
Fragments arranged by size:
4435 427 189 141 92 30 30 30
30 24 6 6 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 685 739 1840 3859 4627 4805 5442
Size: 685 54 1101 2019 768 178 637
Fragments arranged by size:
2019 1101 768 685 637 178 54
BseYI C'CCAG_C
Cuts at: 0 109 2564 5038 5058 5079 5425 5442
Size: 109 2455 2474 20 21 346 17
Fragments arranged by size:
2474 2455 346 109 21 20 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 812 1284 2638 2687 2719 3435 4627
Size: 478 334 472 1354 49 32 716 1192
Cuts at: 4627 5442
Size: 815
Fragments arranged by size:
1354 1192 815 716 478 472 334 49
32
BsiHKAI G_wGCw'C
Cuts at: 0 171 317 776 1553 1585 3758 4478 4712
Size: 171 146 459 777 32 2173 720 234
Cuts at: 4712 5116 5442
Size: 404 326
Fragments arranged by size:
2173 777 720 459 404 326 234 171
146 32
BsmI GAATG_Cn'
Cuts at: 0 266 2314 3027 5442
Size: 266 2048 713 2415
Fragments arranged by size:
2415 2048 713 266
BsmBI CGTCTCn'nnnn_
Cuts at: 0 550 2863 5442
Size: 550 2313 2579
Fragments arranged by size:
2579 2313 550
Bsp1286I G_dGCh'C
Cuts at: 0 171 317 776 1553 1585 2286 2591 3223
Size: 171 146 459 777 32 701 305 632
Cuts at: 3223 3459 3758 4478 4712 5116 5197 5406 5442
Size: 236 299 720 234 404 81 209 36
Fragments arranged by size:
777 720 701 632 459 404 305 299
236 234 209 171 146 81 36 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 400 453 1745 2333 2651 2741 5442
Size: 400 53 1292 588 318 90 2701
Fragments arranged by size:
2701 1292 588 400 318 90 53
BsrDI GCAATG_nn'
Cuts at: 0 1347 1373 4397 5442
Size: 1347 26 3024 1045
Fragments arranged by size:
3024 1347 1045 26
BsrGI T'GTAC_A
Cuts at: 0 2499 5442
Size: 2499 2943
BssSI C'ACGA_G
Cuts at: 0 616 4112 5442
Size: 616 3496 1330
Fragments arranged by size:
3496 1330 616
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1791 2742 3528 4635 5442
Size: 1791 951 786 1107 807
Fragments arranged by size:
1791 1107 951 807 786
BstBI TT'CG_AA
Cuts at: 0 4206 5442
Size: 4206 1236
BstEII G'GTnAC_C
Cuts at: 0 5235 5442
Size: 5235 207
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2065 2293 3867 5442
Size: 2065 228 1574 1575
Fragments arranged by size:
2065 1575 1574 228
BstYI r'GATC_y
Cuts at: 0 471 872 1542 2900 3677 3705 4279 4405
Size: 471 401 670 1358 777 28 574 126
Cuts at: 4405 5442
Size: 1037
Fragments arranged by size:
1358 1037 777 670 574 471 401 126
28
BstZ17I GTA'TAC
Cuts at: 0 2003 5442
Size: 2003 3439
BtgI C'CryG_G
Cuts at: 0 971 2630 3789 4742 5442
Size: 971 1659 1159 953 700
Fragments arranged by size:
1659 1159 971 953 700
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 646 5442
Size: 646 4796
BtsI GCAGTG_nn'
Cuts at: 0 63 2699 2987 3423 4793 5112 5442
Size: 63 2636 288 436 1370 319 330
Fragments arranged by size:
2636 1370 436 330 319 288 63
DraI TTT'AAA
Cuts at: 0 2934 5442
Size: 2934 2508
DraIII CAC_nnn'GTG
Cuts at: 0 2742 3528 4883 5442
Size: 2742 786 1355 559
Fragments arranged by size:
2742 1355 786 559
DrdI GACnn_nn'nnGTC
Cuts at: 0 31 3294 5442
Size: 31 3263 2148
Fragments arranged by size:
3263 2148 31
EaeI y'GGCC_r
Cuts at: 0 2627 2722 3786 3881 4587 4905 4932 5117
Size: 2627 95 1064 95 706 318 27 185
Cuts at: 5117 5187 5442
Size: 70 255
Fragments arranged by size:
2627 1064 706 318 255 185 95 95
70 27
EarI CTCTTCn'nnn_
Cuts at: 0 14 575 685 881 1279 1590 1646 2678
Size: 14 561 110 196 398 311 56 1032
Cuts at: 2678 4700 5129 5442
Size: 2022 429 313
Fragments arranged by size:
2022 1032 561 429 398 313 311 196
110 56 14
EcoICRI GAG'CTC
Cuts at: 0 315 774 1551 3756 5442
Size: 315 459 777 2205 1686
Fragments arranged by size:
2205 1686 777 459 315
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 358 425 788 1118 1273 1285 1462
Size: 334 24 67 363 330 155 12 177
Cuts at: 1462 1801 2041 2465 2566 2944 2996 3120 3212
Size: 339 240 424 101 378 52 124 92
Cuts at: 3212 3227 3616 3637 3946 4043 4133 4437 4465
Size: 15 389 21 309 97 90 304 28
Cuts at: 4465 4492 4639 4702 5173 5184 5404 5442
Size: 27 147 63 471 11 220 38
Fragments arranged by size:
471 424 389 378 363 339 334 330
309 304 240 220 177 155 147 124
101 97 92 90 67 63 52 38
28 27 24 21 15 12 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 721 4954 5134 5365 5442
Size: 721 4233 180 231 77
Fragments arranged by size:
4233 721 231 180 77
EcoO109I rG'GnC_Cy
Cuts at: 0 891 1059 1260 2282 3455 4380 4968 5442
Size: 891 168 201 1022 1173 925 588 474
Fragments arranged by size:
1173 1022 925 891 588 474 201 168
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1972 2512 2693 2826 4122 5442
Size: 1972 540 181 133 1296 1320
Fragments arranged by size:
1972 1320 1296 540 181 133
HaeII r_GCGC'y
Cuts at: 0 5316 5442
Size: 5316 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 715 729 745 747 761 777 1425 1457
Size: 715 14 16 2 14 16 648 32
Cuts at: 1457 1873 1905 3669 3701 4825 4831 4857 4863
Size: 416 32 1764 32 1124 6 26 6
Cuts at: 4863 4985 5017 5442
Size: 122 32 425
Fragments arranged by size:
1764 1124 715 648 425 416 122 32
32 32 32 26 16 16 14 14
6 6 2
HincII GTy'rAC
Cuts at: 0 3814 5442
Size: 3814 1628
HindIII A'AGCT_T
Cuts at: 0 592 701 5155 5293 5442
Size: 592 109 4454 138 149
Fragments arranged by size:
4454 592 149 138 109
MfeI C'AATT_G
Cuts at: 0 5338 5442
Size: 5338 104
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3945 4247 5442
Size: 3945 302 1195
Fragments arranged by size:
3945 1195 302
MscI TGG'CCA
Cuts at: 0 2629 3788 4907 5189 5442
Size: 2629 1159 1119 282 253
Fragments arranged by size:
2629 1159 1119 282 253
MslI CAynn'nnrTG
Cuts at: 0 2456 5442
Size: 2456 2986
MspA1I CmG'CkG
Cuts at: 0 50 366 429 2889 3564 3615 3903 4485
Size: 50 316 63 2460 675 51 288 582
Cuts at: 4485 4563 4580 4586 4635 5304 5442
Size: 78 17 6 49 669 138
Fragments arranged by size:
2460 675 669 582 316 288 138 78
63 51 50 49 17 6
NcoI C'CATG_G
Cuts at: 0 971 3789 4742 5442
Size: 971 2818 953 700
Fragments arranged by size:
2818 971 953 700
NheI G'CTAG_C
Cuts at: 0 2113 5442
Size: 2113 3329
NspI r_CATG'y
Cuts at: 0 746 3663 5420 5442
Size: 746 2917 1757 22
Fragments arranged by size:
2917 1757 746 22
PasI CC'CwG_GG
Cuts at: 0 37 5442
Size: 37 5405
PflMI CCAn_nnn'nTGG
Cuts at: 0 2877 2904 3354 4825 4944 5442
Size: 2877 27 450 1471 119 498
Fragments arranged by size:
2877 1471 498 450 119 27
PfoI T'CCnGG_A
Cuts at: 0 350 644 3893 4453 4568 5409 5442
Size: 350 294 3249 560 115 841 33
Fragments arranged by size:
3249 841 560 350 294 115 33
PmlI CAC'GTG
Cuts at: 0 3546 5442
Size: 3546 1896
PpuMI rG'GwC_Cy
Cuts at: 0 891 1260 5442
Size: 891 369 4182
Fragments arranged by size:
4182 891 369
PshAI GACnn'nnGTC
Cuts at: 0 2085 5442
Size: 2085 3357
PspOMI G'GGCC_C
Cuts at: 0 2282 3455 5442
Size: 2282 1173 1987
Fragments arranged by size:
2282 1987 1173
PstI C_TGCA'G
Cuts at: 0 58 1539 1715 1742 2894 2915 3641 3692
Size: 58 1481 176 27 1152 21 726 51
Cuts at: 3692 3857 4526 4585 5442
Size: 165 669 59 857
Fragments arranged by size:
1481 1152 857 726 669 176 165 59
58 51 27 21
PvuII CAG'CTG
Cuts at: 0 366 2889 3615 3903 4563 4635 5304 5442
Size: 366 2523 726 288 660 72 669 138
Fragments arranged by size:
2523 726 669 660 366 288 138 72
RsrII CG'GwC_CG
Cuts at: 0 3559 5442
Size: 3559 1883
SacI G_AGCT'C
Cuts at: 0 317 776 1553 3758 5442
Size: 317 459 777 2205 1684
Fragments arranged by size:
2205 1684 777 459 317
SapI GCTCTTCn'nnn_
Cuts at: 0 575 685 1279 1590 1646 4700 5442
Size: 575 110 594 311 56 3054 742
Fragments arranged by size:
3054 742 594 575 311 110 56
SbfI CC_TGCA'GG
Cuts at: 0 1742 5442
Size: 1742 3700
SfcI C'TryA_G
Cuts at: 0 54 920 1063 1535 1711 1738 2090 2890
Size: 54 866 143 472 176 27 352 800
Cuts at: 2890 2911 3637 3688 3853 4522 4581 5142 5442
Size: 21 726 51 165 669 59 561 300
Fragments arranged by size:
866 800 726 669 561 472 352 300
176 165 143 59 54 51 27 21
SmlI C'TyrA_G
Cuts at: 0 250 316 1546 1946 1957 2244 3468 4172
Size: 250 66 1230 400 11 287 1224 704
Cuts at: 4172 4362 5442
Size: 190 1080
Fragments arranged by size:
1230 1224 1080 704 400 287 250 190
66 11
StuI AGG'CCT
Cuts at: 0 541 5140 5394 5442
Size: 541 4599 254 48
Fragments arranged by size:
4599 541 254 48
StyI C'CwwG_G
Cuts at: 0 796 971 1820 2591 3789 4742 5442
Size: 796 175 849 771 1198 953 700
Fragments arranged by size:
1198 953 849 796 771 700 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1228 2738 2884 3547 3997 5093 5442
Size: 1228 1510 146 663 450 1096 349
Fragments arranged by size:
1510 1228 1096 663 450 349 146
TatI w'GTAC_w
Cuts at: 0 1171 2499 5442
Size: 1171 1328 2943
Fragments arranged by size:
2943 1328 1171
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5176 5208 5442
Size: 5176 32 234
Fragments arranged by size:
5176 234 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 111 2361 2874 5127 5379 5442
Size: 111 2250 513 2253 252 63
Fragments arranged by size:
2253 2250 513 252 111 63
XmnI GAAnn'nnTTC
Cuts at: 0 2405 3212 3697 4289 5442
Size: 2405 807 485 592 1153
Fragments arranged by size:
2405 1153 807 592 485
ZraI GAC'GTC
Cuts at: 0 27 5442
Size: 27 5415
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflII AflIII AlfI
AloI AlwNI ApaI ApoI AseI AvaI BamHI BanI
BanII BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrDI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII HindIII MfeI MmeI MscI
MslI MspA1I NcoI NheI NspI PasI PflMI PfoI
PmlI PpuMI PshAI PspOMI PstI PvuII RsrII SacI
SapI SbfI SfcI SmlI StuI StyI TaqII TatI
TstI XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AgeI AhdI AleI ApaLI AscI AsiSI
AvrII BaeI BbeI BbvCI BcgI BfrBI BsaBI BsaWI
BsiEI BsiWI BspEI BspHI BsrBI BsrFI BssHII Bsu36I
ClaI CspCI EagI EciI EcoRI EcoRV FseI FspI
FspAI HpaI KasI KpnI MluI NaeI NarI NdeI
NgoMIV NotI NruI NsiI PacI PciI PmeI PpiI
PsiI PspXI PsrI PvuI SacII SalI SanDI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI