(Circular) (Six-base) MAPSORT of: pFN21AB6312.seq Check: 3511 from: 1 to: 5545 pFN21AB6312 5545 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3355 278 Size: 53 83 186 936 1819 2468 Fragments arranged by size: 2468 1819 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2888 1937 Size: 951 4594 Acc65I G'GTAC_C Cuts at: 2866 2866 Size: 5545 AclI AA'CG_TT Cuts at: 1563 3672 4045 1563 Size: 2109 373 3063 Fragments arranged by size: 3063 2109 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3608 4992 5260 1632 Size: 1976 1384 268 1917 Fragments arranged by size: 1976 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5348 AflIII A'CryG_T Cuts at: 1203 4444 5421 1203 Size: 3241 977 1327 Fragments arranged by size: 3241 1327 977 AgeI A'CCGG_T Cuts at: 4376 4376 Size: 5545 AhdI GACnn_n'nnGTC Cuts at: 4274 4274 Size: 5545 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5545 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2772 1184 Size: 1588 3957 AlwNI CAG_nnn'CTG Cuts at: 2251 3288 4860 2251 Size: 1037 1572 2936 Fragments arranged by size: 2936 1572 1037 ApaLI G'TGCA_C Cuts at: 3602 4758 3602 Size: 1156 4389 ApoI r'AATT_y Cuts at: 1493 2854 3080 3232 5341 1493 Size: 1361 226 152 2109 1697 Fragments arranged by size: 2109 1697 1361 226 152 AseI AT'TA_AT Cuts at: 160 1032 4099 160 Size: 872 3067 1606 Fragments arranged by size: 3067 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5545 AvaI C'yCGr_G Cuts at: 1870 1943 2870 1870 Size: 73 927 4545 Fragments arranged by size: 4545 927 73 AvrII C'CTAG_G Cuts at: 2012 2012 Size: 5545 BamHI G'GATC_C Cuts at: 2767 2875 3242 2767 Size: 108 367 5070 Fragments arranged by size: 5070 367 108 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2866 4321 618 Size: 325 217 627 1079 1455 1842 Fragments arranged by size: 1842 1455 1079 627 325 217 BanII G_rGCy'C Cuts at: 1391 2864 1391 Size: 1473 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2584 928 Size: 971 685 3889 Fragments arranged by size: 3889 971 685 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3736 3770 1903 Size: 34 1799 34 3678 Fragments arranged by size: 3678 1799 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3437 4647 3437 Size: 1210 4335 BclI T'GATC_A Cuts at: 1548 1548 Size: 5545 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4156 136 Size: 107 122 71 631 605 2484 1525 Fragments arranged by size: 2484 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2682 5540 2682 Size: 2858 2687 BlpI GC'TnA_GC Cuts at: 2962 2962 Size: 5545 Bme1580I G_kGCm'C Cuts at: 1790 3606 4762 1790 Size: 1816 1156 2573 Fragments arranged by size: 2573 1816 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5545 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3276 4234 442 Size: 416 53 83 399 24 1859 958 1753 Fragments arranged by size: 1859 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5545 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2544 2601 4205 1239 Size: 1305 57 1604 2579 Fragments arranged by size: 2579 1604 1305 57 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2129 2569 3670 4535 4833 2129 Size: 440 1101 865 298 2841 Fragments arranged by size: 2841 1101 865 440 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4208 882 Size: 617 2709 2219 Fragments arranged by size: 2709 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3336 493 Size: 711 2132 2702 Fragments arranged by size: 2702 2132 711 BsaBI GATnn'nnATC Cuts at: 5539 5539 Size: 5545 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3352 3734 4369 275 Size: 53 83 186 936 1819 382 635 1451 Fragments arranged by size: 1819 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3978 4376 4650 4797 5208 3978 Size: 398 274 147 411 4315 Fragments arranged by size: 4315 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5320 Fragments arranged by size: 5320 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2132 1186 Size: 946 4599 BseYI C'CCAG_C Cuts at: 2518 2760 4748 5244 2518 Size: 242 1988 496 2819 Fragments arranged by size: 2819 1988 496 242 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4972 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2092 2995 3756 4784 1540 Size: 275 186 91 903 761 1028 2301 Fragments arranged by size: 2301 1028 903 761 275 186 91 BsiHKAI G_wGCw'C Cuts at: 3606 3691 4762 3606 Size: 85 1071 4389 Fragments arranged by size: 4389 1071 85 BsmI GAATG_Cn' Cuts at: 3056 3149 3056 Size: 93 5452 BsmBI CGTCTCn'nnnn_ Cuts at: 2813 2813 Size: 5545 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2864 3606 3691 4762 1391 Size: 399 1074 742 85 1071 2174 Fragments arranged by size: 2174 1074 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5208 5208 Size: 5545 BspHI T'CATG_A Cuts at: 3434 5164 3434 Size: 1730 3815 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2900 844 Size: 2056 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2632 3432 1394 Size: 429 809 800 3507 Fragments arranged by size: 3507 809 800 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4040 4214 65 Size: 1183 2792 174 1396 Fragments arranged by size: 2792 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4189 4376 1700 Size: 110 2379 187 2869 Fragments arranged by size: 2869 2379 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5545 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5545 BssSI C'ACGA_G Cuts at: 3605 4617 3605 Size: 1012 4533 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5545 BstBI TT'CG_AA Cuts at: 4349 4349 Size: 5545 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5545 BstYI r'GATC_y Cuts at: 1972 2175 2682 2767 2875 3242 3627 3644 5096 Size: 203 507 85 108 367 385 17 1452 Cuts at: 5096 5182 5194 5540 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 507 385 367 346 203 108 86 85 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2523 2544 513 Size: 552 939 519 21 3514 Fragments arranged by size: 3514 939 552 519 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4806 Fragments arranged by size: 4806 683 56 BtsI GCAGTG_nn' Cuts at: 3061 3855 3875 3061 Size: 794 20 4731 Fragments arranged by size: 4731 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5545 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5510 DraI TTT'AAA Cuts at: 2849 2849 Size: 5545 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2588 4552 809 Size: 507 1272 1964 1802 Fragments arranged by size: 1964 1802 1272 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2730 2992 Size: 54 1425 50 275 18 204 696 262 Cuts at: 2992 3881 5510 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 696 275 262 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2992 1537 Size: 275 1180 4090 Fragments arranged by size: 4090 1180 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2250 2373 2447 3475 1353 Size: 897 123 74 1028 3423 Fragments arranged by size: 3423 1028 897 123 74 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2074 2724 4132 4506 4652 387 Size: 1064 138 485 650 1408 374 146 1280 Fragments arranged by size: 1408 1280 1064 650 485 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2544 2601 3608 4205 4992 5260 1239 Size: 393 912 57 1007 597 787 268 1524 Fragments arranged by size: 1524 1007 912 787 597 393 268 57 EcoNI CCTnn'n_nnAGG Cuts at: 2053 2144 2147 2255 2053 Size: 91 3 108 5343 Fragments arranged by size: 5343 108 91 3 EcoO109I rG'GnC_Cy Cuts at: 2248 2248 Size: 5545 EcoRI G'AATT_C Cuts at: 2854 3232 2854 Size: 378 5167 EcoRV GAT'ATC Cuts at: 2281 2281 Size: 5545 FspI TGC'GCA Cuts at: 4051 4051 Size: 5545 HaeII r_GCGC'y Cuts at: 1678 4692 1678 Size: 3014 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4186 4218 4260 4292 1373 Size: 32 135 32 2614 32 42 32 2626 Fragments arranged by size: 2626 2614 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2889 3135 669 Size: 988 281 951 246 3079 Fragments arranged by size: 3079 988 951 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5545 HpaI GTT'AAC Cuts at: 3135 3135 Size: 5545 KpnI G_GTAC'C Cuts at: 2870 2870 Size: 5545 MfeI C'AATT_G Cuts at: 3144 3144 Size: 5545 MluI A'CGCG_T Cuts at: 5421 5421 Size: 5545 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2826 4659 4843 1305 Size: 1521 1833 184 2007 Fragments arranged by size: 2007 1833 1521 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2036 2732 10 Size: 54 1425 343 204 696 2823 Fragments arranged by size: 2823 1425 696 343 204 54 MslI CAynn'nnrTG Cuts at: 518 1166 3503 3862 4021 518 Size: 648 2337 359 159 2042 Fragments arranged by size: 2337 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2270 2764 2961 3638 4786 5031 1606 Size: 664 494 197 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 664 494 245 197 NcoI C'CATG_G Cuts at: 513 1065 2004 2544 513 Size: 552 939 540 3514 Fragments arranged by size: 3514 939 552 540 NdeI CA'TA_TG Cuts at: 387 387 Size: 5545 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5545 NotI GC'GGCC_GC Cuts at: 2992 2992 Size: 5545 NspI r_CATG'y Cuts at: 1132 1229 2903 4448 1132 Size: 97 1674 1545 2229 Fragments arranged by size: 2229 1674 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5545 PciI A'CATG_T Cuts at: 4444 4444 Size: 5545 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5545 PmeI GTTT'AAAC Cuts at: 2849 2849 Size: 5545 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3614 3646 5127 5159 3614 Size: 32 1481 32 4000 Fragments arranged by size: 4000 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5545 PsiI TTA'TAA Cuts at: 3115 3115 Size: 5545 PstI C_TGCA'G Cuts at: 830 2825 2897 830 Size: 1995 72 3478 Fragments arranged by size: 3478 1995 72 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5545 PvuII CAG'CTG Cuts at: 2270 2764 2270 Size: 494 5051 SalI G'TCGA_C Cuts at: 1936 2887 1936 Size: 951 4594 SbfI CC_TGCA'GG Cuts at: 2897 2897 Size: 5545 ScaI AGT'ACT Cuts at: 3793 5505 3793 Size: 1712 3833 SfcI C'TryA_G Cuts at: 826 1045 2821 2893 4028 4709 4900 826 Size: 219 1776 72 1135 681 191 1471 Fragments arranged by size: 1776 1471 1135 681 219 191 72 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5545 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5545 SmaI CCC'GGG Cuts at: 1872 2872 1872 Size: 1000 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2144 2584 3649 4550 4812 820 Size: 197 926 201 440 1065 901 262 1553 Fragments arranged by size: 1553 1065 926 901 440 262 201 197 SnaBI TAC'GTA Cuts at: 493 3336 493 Size: 2843 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5545 SphI G_CATG'C Cuts at: 1132 2903 1132 Size: 1771 3774 SspI AAT'ATT Cuts at: 5 52 3469 5 Size: 47 3417 2081 Fragments arranged by size: 3417 2081 47 StuI AGG'CCT Cuts at: 1517 2565 2777 1517 Size: 1048 212 4285 Fragments arranged by size: 4285 1048 212 StyI C'CwwG_G Cuts at: 513 1065 2004 2012 2190 2230 2544 2778 2984 Size: 552 939 8 178 40 314 234 206 Cuts at: 2984 513 Size: 3074 Fragments arranged by size: 3074 939 552 314 234 206 178 40 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2587 3572 3589 3742 3927 4372 325 Size: 1255 1007 985 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1007 985 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3791 5503 96 Size: 274 80 33 51 1443 1814 1712 138 Fragments arranged by size: 1814 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5505 5537 5505 Size: 32 5513 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5545 XbaI T'CTAG_A Cuts at: 2881 2881 Size: 5545 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5545 XmaI C'CCGG_G Cuts at: 1870 2870 1870 Size: 1000 4545 XmnI GAAnn'nnTTC Cuts at: 2084 2313 3674 2084 Size: 229 1361 3955 Fragments arranged by size: 3955 1361 229 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3353 276 Size: 53 83 186 936 1819 2468 Fragments arranged by size: 2468 1819 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI