(Circular) (Six-base) MAPSORT of: pFN21AA0432.seq Check: 7756 from: 1 to: 7111 pFN21AA0432 7111 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2548 4921 278 Size: 53 83 186 936 1012 2373 2468 Fragments arranged by size: 2468 2373 1012 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4454 1937 Size: 2517 4594 Acc65I G'GTAC_C Cuts at: 3821 4432 3821 Size: 611 6500 AclI AA'CG_TT Cuts at: 1563 5238 5611 1563 Size: 3675 373 3063 Fragments arranged by size: 3675 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2484 3285 5174 6558 6826 1632 Size: 852 801 1889 1384 268 1917 Fragments arranged by size: 1917 1889 1384 852 801 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6914 AflIII A'CryG_T Cuts at: 1203 6010 6987 1203 Size: 4807 977 1327 Fragments arranged by size: 4807 1327 977 AgeI A'CCGG_T Cuts at: 5942 5942 Size: 7111 AhdI GACnn_n'nnGTC Cuts at: 5840 5840 Size: 7111 AleI CACnn'nnGTG Cuts at: 1166 3125 1166 Size: 1959 5152 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2155 2305 1184 Size: 971 150 5990 Fragments arranged by size: 5990 971 150 AlwNI CAG_nnn'CTG Cuts at: 4854 6426 4854 Size: 1572 5539 ApaLI G'TGCA_C Cuts at: 5168 6324 5168 Size: 1156 5955 ApoI r'AATT_y Cuts at: 1493 2055 2306 2595 2864 2995 3087 4158 4406 Size: 562 251 289 269 131 92 1071 248 Cuts at: 4406 4420 4646 4798 6907 1493 Size: 14 226 152 2109 1697 Fragments arranged by size: 2109 1697 1071 562 289 269 251 248 226 152 131 92 14 AseI AT'TA_AT Cuts at: 160 1032 3354 5665 160 Size: 872 2322 2311 1606 Fragments arranged by size: 2322 2311 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7111 AvaI C'yCGr_G Cuts at: 1870 1943 3359 3420 4091 4436 1870 Size: 73 1416 61 671 345 4545 Fragments arranged by size: 4545 1416 671 345 73 61 BamHI G'GATC_C Cuts at: 2167 4441 4808 2167 Size: 2274 367 4470 Fragments arranged by size: 4470 2274 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3821 4432 5887 618 Size: 325 217 627 2034 611 1455 1842 Fragments arranged by size: 2034 1842 1455 627 611 325 217 BanII G_rGCy'C Cuts at: 1391 2476 3960 4197 4430 1391 Size: 1085 1484 237 233 4072 Fragments arranged by size: 4072 1484 1085 237 233 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2189 4326 928 Size: 971 290 2137 3713 Fragments arranged by size: 3713 2137 971 290 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5302 5336 1903 Size: 34 50 34 3281 34 3678 Fragments arranged by size: 3678 3281 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5003 6213 5003 Size: 1210 5901 BclI T'GATC_A Cuts at: 1548 2019 3741 1548 Size: 471 1722 4918 Fragments arranged by size: 4918 1722 471 BfrBI ATG'CAT Cuts at: 3616 3616 Size: 7111 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2480 4049 4130 Size: 107 122 71 631 605 808 1569 81 Cuts at: 4130 5722 136 Size: 1592 1525 Fragments arranged by size: 1592 1569 1525 808 631 605 122 107 81 71 BglII A'GATC_T Cuts at: 2759 3634 7106 2759 Size: 875 3472 2764 Fragments arranged by size: 3472 2764 875 BlpI GC'TnA_GC Cuts at: 4528 4528 Size: 7111 Bme1580I G_kGCm'C Cuts at: 1790 5172 6328 1790 Size: 3382 1156 2573 Fragments arranged by size: 3382 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2966 1318 Size: 1648 5463 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2281 4842 5800 Size: 416 53 83 399 24 864 2561 958 Cuts at: 5800 442 Size: 1753 Fragments arranged by size: 2561 1753 958 864 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7111 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3446 3446 Size: 7111 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2400 2909 4403 5771 1239 Size: 1161 509 1494 1368 2579 Fragments arranged by size: 2579 1494 1368 1161 509 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2471 3946 4253 5236 6101 6399 2471 Size: 1475 307 983 865 298 3183 Fragments arranged by size: 3183 1475 983 865 307 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5774 882 Size: 617 4275 2219 Fragments arranged by size: 4275 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3397 4902 493 Size: 711 2193 1505 2702 Fragments arranged by size: 2702 2193 1505 711 BsaBI GATnn'nnATC Cuts at: 2394 7105 2394 Size: 4711 2400 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2545 4918 5300 5935 Size: 53 83 186 936 1012 2373 382 635 Cuts at: 5935 275 Size: 1451 Fragments arranged by size: 2373 1451 1012 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5544 5942 6216 6363 6774 5544 Size: 398 274 147 411 5881 Fragments arranged by size: 5881 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2245 2275 1178 Size: 30 165 30 842 30 6014 Fragments arranged by size: 6014 842 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2637 3321 1186 Size: 1451 684 4976 Fragments arranged by size: 4976 1451 684 BseYI C'CCAG_C Cuts at: 6314 6810 6314 Size: 496 6615 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3935 1163 Size: 573 2199 4339 Fragments arranged by size: 4339 2199 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4561 5322 6350 1540 Size: 275 186 2560 761 1028 2301 Fragments arranged by size: 2560 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3840 3960 5172 5257 6328 3840 Size: 120 1212 85 1071 4623 Fragments arranged by size: 4623 1212 1071 120 85 BsmI GAATG_Cn' Cuts at: 3618 3998 4622 4715 3618 Size: 380 624 93 6014 Fragments arranged by size: 6014 624 380 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2476 3840 3960 4197 4430 5172 5257 Size: 399 686 1364 120 237 233 742 85 Cuts at: 5257 6328 1391 Size: 1071 2174 Fragments arranged by size: 2174 1364 1071 742 686 399 237 233 120 85 BspEI T'CCGG_A Cuts at: 6774 6774 Size: 7111 BspHI T'CATG_A Cuts at: 4197 5000 6730 4197 Size: 803 1730 4578 Fragments arranged by size: 4578 1730 803 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4466 844 Size: 3622 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4998 1394 Size: 429 3175 3507 Fragments arranged by size: 3507 3175 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2111 2256 3161 5606 5780 65 Size: 1183 863 145 905 2445 174 1396 Fragments arranged by size: 2445 1396 1183 905 863 174 145 BsrFI r'CCGG_y Cuts at: 1700 1810 5755 5942 1700 Size: 110 3945 187 2869 Fragments arranged by size: 3945 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3208 96 Size: 3112 3999 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7111 BssSI C'ACGA_G Cuts at: 4182 5171 6183 4182 Size: 989 1012 5110 Fragments arranged by size: 5110 1012 989 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7111 BstBI TT'CG_AA Cuts at: 5915 5915 Size: 7111 BstEII G'GTnAC_C Cuts at: 1184 4172 1184 Size: 2988 4123 BstXI CCAn_nnnn'nTGG Cuts at: 2247 2247 Size: 7111 BstYI r'GATC_y Cuts at: 1972 2167 2389 2740 2759 3634 4441 4808 5193 Size: 195 222 351 19 875 807 367 385 Cuts at: 5193 5210 6662 6748 6760 7106 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 875 807 385 367 351 346 222 195 86 19 17 12 BtgI C'CryG_G Cuts at: 513 1065 3949 513 Size: 552 2884 3675 Fragments arranged by size: 3675 2884 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6372 Fragments arranged by size: 6372 683 56 BtsI GCAGTG_nn' Cuts at: 2144 4627 5421 5441 2144 Size: 2483 794 20 3814 Fragments arranged by size: 3814 2483 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7111 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7076 DraI TTT'AAA Cuts at: 4398 4415 4398 Size: 17 7094 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2191 4399 6118 809 Size: 507 875 2208 1719 1802 Fragments arranged by size: 2208 1802 1719 875 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2226 4558 5447 Size: 54 1425 50 275 18 396 2332 889 Cuts at: 5447 7076 8 Size: 1629 43 Fragments arranged by size: 2332 1629 1425 889 396 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4558 1537 Size: 275 2746 4090 Fragments arranged by size: 4090 2746 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2199 2337 2563 2612 2770 3339 3879 5041 Size: 846 138 226 49 158 569 540 1162 Cuts at: 5041 1353 Size: 3423 Fragments arranged by size: 3423 1162 846 569 540 226 158 138 49 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5698 6072 6218 387 Size: 1064 138 4109 374 146 1280 Fragments arranged by size: 4109 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3958 3958 Size: 7111 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2400 2484 2909 3285 4403 5174 5771 Size: 393 768 84 425 376 1118 771 597 Cuts at: 5771 6558 6826 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1118 787 771 768 597 425 393 376 268 84 EcoRI G'AATT_C Cuts at: 3087 4420 4798 3087 Size: 1333 378 5400 Fragments arranged by size: 5400 1333 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3167 3987 3167 Size: 820 6291 FspI TGC'GCA Cuts at: 5617 5617 Size: 7111 HaeII r_GCGC'y Cuts at: 1678 6258 1678 Size: 4580 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2227 2245 2259 2277 2744 Size: 32 135 32 655 18 14 18 467 Cuts at: 2744 2776 2816 2848 5752 5784 5826 5858 1373 Size: 32 40 32 2904 32 42 32 2626 Fragments arranged by size: 2904 2626 655 467 135 42 40 32 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 4455 4701 669 Size: 988 281 2517 246 3079 Fragments arranged by size: 3079 2517 988 281 246 HindIII A'AGCT_T Cuts at: 748 3969 748 Size: 3221 3890 HpaI GTT'AAC Cuts at: 4701 4701 Size: 7111 KpnI G_GTAC'C Cuts at: 3825 4436 3825 Size: 611 6500 MfeI C'AATT_G Cuts at: 2427 2528 4710 2427 Size: 101 2182 4828 Fragments arranged by size: 4828 2182 101 MluI A'CGCG_T Cuts at: 6987 6987 Size: 7111 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6225 6409 1305 Size: 4920 184 2007 Fragments arranged by size: 4920 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2228 10 Size: 54 1425 343 396 4893 Fragments arranged by size: 4893 1425 396 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3125 3171 3777 4365 5069 5428 5587 Size: 648 1959 46 606 588 704 359 159 Cuts at: 5587 518 Size: 2042 Fragments arranged by size: 2042 1959 704 648 606 588 359 159 46 MspA1I CmG'CkG Cuts at: 1606 2069 2585 4527 5204 6352 6597 1606 Size: 463 516 1942 677 1148 245 2120 Fragments arranged by size: 2120 1942 1148 677 516 463 245 NcoI C'CATG_G Cuts at: 513 1065 3949 513 Size: 552 2884 3675 Fragments arranged by size: 3675 2884 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7111 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7111 NotI GC'GGCC_GC Cuts at: 4558 4558 Size: 7111 NsiI A_TGCA'T Cuts at: 3618 3618 Size: 7111 NspI r_CATG'y Cuts at: 1132 1229 4469 6014 1132 Size: 97 3240 1545 2229 Fragments arranged by size: 3240 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7111 PciI A'CATG_T Cuts at: 6010 6010 Size: 7111 PflMI CCAn_nnn'nTGG Cuts at: 1108 2270 1108 Size: 1162 5949 PfoI T'CCnGG_A Cuts at: 2925 2925 Size: 7111 PmeI GTTT'AAAC Cuts at: 4415 4415 Size: 7111 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5180 5212 6693 6725 5180 Size: 32 1481 32 5566 Fragments arranged by size: 5566 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7111 PsiI TTA'TAA Cuts at: 2634 3975 4681 2634 Size: 1341 706 5064 Fragments arranged by size: 5064 1341 706 PstI C_TGCA'G Cuts at: 830 3096 4463 830 Size: 2266 1367 3478 Fragments arranged by size: 3478 2266 1367 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7111 PvuII CAG'CTG Cuts at: 2585 2585 Size: 7111 SacI G_AGCT'C Cuts at: 3960 3960 Size: 7111 SalI G'TCGA_C Cuts at: 1936 4453 1936 Size: 2517 4594 SapI GCTCTTCn'nnn_ Cuts at: 3879 3879 Size: 7111 SbfI CC_TGCA'GG Cuts at: 4463 4463 Size: 7111 ScaI AGT'ACT Cuts at: 2892 5359 7071 2892 Size: 2467 1712 2932 Fragments arranged by size: 2932 2467 1712 SexAI A'CCwGG_T Cuts at: 3978 3978 Size: 7111 SfcI C'TryA_G Cuts at: 826 1045 3092 3379 3810 3997 4459 5594 6275 Size: 219 2047 287 431 187 462 1135 681 Cuts at: 6275 6466 826 Size: 191 1471 Fragments arranged by size: 2047 1471 1135 681 462 431 287 219 191 187 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7111 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7111 SmaI CCC'GGG Cuts at: 1872 4438 1872 Size: 2566 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2450 3961 4091 4268 5215 6116 Size: 197 926 507 1511 130 177 947 901 Cuts at: 6116 6378 820 Size: 262 1553 Fragments arranged by size: 1553 1511 947 926 901 507 262 197 177 130 SnaBI TAC'GTA Cuts at: 493 4902 493 Size: 4409 2702 SpeI A'CTAG_T Cuts at: 152 2849 2887 152 Size: 2697 38 4376 Fragments arranged by size: 4376 2697 38 SphI G_CATG'C Cuts at: 1132 4469 1132 Size: 3337 3774 SspI AAT'ATT Cuts at: 5 52 5035 5 Size: 47 4983 2081 Fragments arranged by size: 4983 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7111 StyI C'CwwG_G Cuts at: 513 1065 2668 3949 4550 513 Size: 552 1603 1281 601 3074 Fragments arranged by size: 3074 1603 1281 601 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5138 5155 5308 5493 5938 325 Size: 1255 3558 17 153 185 445 1498 Fragments arranged by size: 3558 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2890 3008 3024 Size: 274 80 33 51 1443 913 118 16 Cuts at: 3024 3208 5357 7069 96 Size: 184 2149 1712 138 Fragments arranged by size: 2149 1712 1443 913 274 184 138 118 80 51 33 16 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7071 7103 7071 Size: 32 7079 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7111 XbaI T'CTAG_A Cuts at: 4447 4447 Size: 7111 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2476 2476 Size: 7111 XhoI C'TCGA_G Cuts at: 1943 4091 1943 Size: 2148 4963 XmaI C'CCGG_G Cuts at: 1870 4436 1870 Size: 2566 4545 XmnI GAAnn'nnTTC Cuts at: 2310 3480 3666 4323 5240 2310 Size: 1170 186 657 917 4181 Fragments arranged by size: 4181 1170 917 657 186 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2546 4919 276 Size: 53 83 186 936 1012 2373 2468 Fragments arranged by size: 2468 2373 1012 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI