Restriction map for pFN21AE0478
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        AocI        
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AvrII       BalI        BamHI       BbrPI       
BbsI        BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BpmI        BpuAI       BsaAI       
BsaBI       BsaWI       BseAI       BseRI       BsiEI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrGI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       FbaI        FseI        HincII      
HindII      HindIII     HpaI        HphI        KpnI        
Ksp632I     MaeIII      MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MunI        
NcoI        NdeI        NotI        NruI        NspV        
PacI        PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tru9I       
Tsp45I      Tsp509I     Van91I      XbaI        XcmI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AflIII        452  ACGTGT               A^C[GA][CT]GT
AlwI          174  GATCC                GGATC(4/5)
AlwNI         310  CAGCTACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         1109  GGGCCC               GGGCC^C
AviII         686  TGCGCA               TGC^GCA
BlpI          307  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      307  GCTCAGC              GC^T[ACGT]AGC
BsaI          187  GGTCTC               GGTCTC(1/5)
BsgI          745  GTGCAG               GTGCAG(16/14)
BspMI         739  GCAGGT               ACCTGC(4/8)
BsrDI         528  CATTGC               GCAATG(2/0)
BssSI        1018  CACGAG               CACGAG(-5/-1)
BstF5I        669  CATCC                GGATG(2/0)
BstYI         173  AGATCC               [GA]^GATC[CT]
CelII         307  GCTCAGC              GC^T[ACGT]AGC
DsaI          235  CCGCGG               C^C[GA][CT]GG
EaeI          665  CGGCCA               [CT]^GGCC[GA]
EcoT14I      1104  CCAAGG               C^C[AT][AT]GG
EcoT22I       813  ATGCAT               ATGCA^T
FokI          669  CATCC                GGATG(9/13)
FspI          686  TGCGCA               TGC^GCA
KspI          235  CCGCGG               CCGC^GG
MboII          88  GAAGA                GAAGA(8/7)
MflI          173  AGATCC               [GA]^GATC[CT]
NaeI          181  GCCGGC               GCC^GGC
NgoMIV        181  GCCGGC               G^CCGGC
NheI          336  GCTAGC               G^CTAGC
NsiI          813  ATGCAT               ATGCA^T
NspI          815  GCATGT               [GA]CATG^[CT]
PaeR7I        367  CTCGAG               C^TCGAG
PpuMI         371  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII         912  CAGCTG               CAG^CTG
SacII         235  CCGCGG               CCGC^GG
SmaI         1112  CCCGGG               CCC^GGG
StuI          510  AGGCCT               AGG^CCT
StyI         1104  CCAAGG               C^C[AT][AT]GG
XhoI          367  CTCGAG               C^TCGAG
XhoII         173  AGATCC               [GA]^GATC[CT]
XmaCI        1112  CCCGGG               C^CCGGG
XmaI         1112  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AfaI          342  GTAC                 GT^AC
              520  GTAC                
Alw44I         43  GTGCAC               G^TGCAC
             1055  GTGCAC              
Aor51HI       681  AGCGCT               AGC^GCT
              802  AGCGCT              
ApaLI          43  GTGCAC               G^TGCAC
             1055  GTGCAC              
Asp700I       562  GAACAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              984  GAATCCCTTC          
AspI          250  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1065  GACCCTGTC           
BanII        1023  GGGCTC               G[GA]GC[CT]^C
             1109  GGGCCC              
BbeI          322  GGCGCC               GGCGC^C
              933  GGCGCC              
BbvCI         121  CCTCAGC              CCTCAGC(-5/-2)
              765  CCTCAGC             
BglI          574  GCCTTCCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              724  GCCGCACTGGC         
BsmBI          48  CGTCTC               CGTCTC(1/5)
              703  GAGACG              
BsrFI         181  GCCGGC               [GA]^CCGG[CT]
              385  ACCGGC              
BssHII          6  GCGCGC               G^CGCGC
              783  GCGCGC              
Cfr10I        181  GCCGGC               [GA]^CCGG[CT]
              385  ACCGGC              
DpnII         174  GATC                 ^GATC
              907  GATC                
DraII         371  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1108  GGGGCCC             
Eco47III      681  AGCGCT               AGC^GCT
              802  AGCGCT              
EcoO109I      371  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1108  GGGGCCC             
FauI          234  CCCGC                CCCGC(4/6)
              347  CCCGC               
HinfI         675  GAATC                G^A[ACGT]TC
              984  GAATC               
KasI          322  GGCGCC               G^GCGCC
              933  GGCGCC              
MboI          174  GATC                 ^GATC
              907  GATC                
MslI          693  CACAGGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              711  CACAGGCGTG          
NarI          322  GGCGCC               GG^CGCC
              933  GGCGCC              
NdeII         174  GATC                 ^GATC
              907  GATC                
PflFI         250  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1065  GACCCTGTC           
PstI          158  CTGCAG               CTGCA^G
              490  CTGCAG              
RsaI          342  GTAC                 GT^AC
              520  GTAC                
Sau3AI        174  GATC                 ^GATC
              907  GATC                
SfaNI         220  GATGC                GCATC(5/9)
              812  GATGC               
SfcI          158  CTGCAG               C^T[GA][CT]AG
              490  CTGCAG              
SfoI          322  GGCGCC               GGC^GCC
              933  GGCGCC              
TfiI          675  GAATC                G^A[AT]TC
              984  GAATC               
Tth111I       250  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1065  GACCCTGTC           
XmnI          562  GAACAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              984  GAATCCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AspHI       
AvaI        AvaII       BanI        BbvI        BcnI        
BfaI        BmyI        BsaHI       BsaJI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrI        BssKI       BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        EcoRII      
Fnu4HI      HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HpaII       ItaI        
MaeI        MaeII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NlaIII      
NlaIV       Sau96I      ScrFI       SmlI        TaqI        
TseI        TspRI       TthHB8I     
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