Restriction map for
pFN21AE0478
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AocI
ApoI AscI AseI AsnI Asp718I
AspEI AvrII BalI BamHI BbrPI
BbsI BcgI BciVI BclI BfrI
BglII BlnI BpmI BpuAI BsaAI
BsaBI BsaWI BseAI BseRI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI HincII
HindII HindIII HpaI HphI KpnI
Ksp632I MaeIII MamI MfeI MluI
MluNI MroI MscI MseI MunI
NcoI NdeI NotI NruI NspV
PacI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tsp45I Tsp509I Van91I XbaI XcmI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflIII 452 ACGTGT A^C[GA][CT]GT
AlwI 174 GATCC GGATC(4/5)
AlwNI 310 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1109 GGGCCC GGGCC^C
AviII 686 TGCGCA TGC^GCA
BlpI 307 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 307 GCTCAGC GC^T[ACGT]AGC
BsaI 187 GGTCTC GGTCTC(1/5)
BsgI 745 GTGCAG GTGCAG(16/14)
BspMI 739 GCAGGT ACCTGC(4/8)
BsrDI 528 CATTGC GCAATG(2/0)
BssSI 1018 CACGAG CACGAG(-5/-1)
BstF5I 669 CATCC GGATG(2/0)
BstYI 173 AGATCC [GA]^GATC[CT]
CelII 307 GCTCAGC GC^T[ACGT]AGC
DsaI 235 CCGCGG C^C[GA][CT]GG
EaeI 665 CGGCCA [CT]^GGCC[GA]
EcoT14I 1104 CCAAGG C^C[AT][AT]GG
EcoT22I 813 ATGCAT ATGCA^T
FokI 669 CATCC GGATG(9/13)
FspI 686 TGCGCA TGC^GCA
KspI 235 CCGCGG CCGC^GG
MboII 88 GAAGA GAAGA(8/7)
MflI 173 AGATCC [GA]^GATC[CT]
NaeI 181 GCCGGC GCC^GGC
NgoMIV 181 GCCGGC G^CCGGC
NheI 336 GCTAGC G^CTAGC
NsiI 813 ATGCAT ATGCA^T
NspI 815 GCATGT [GA]CATG^[CT]
PaeR7I 367 CTCGAG C^TCGAG
PpuMI 371 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 912 CAGCTG CAG^CTG
SacII 235 CCGCGG CCGC^GG
SmaI 1112 CCCGGG CCC^GGG
StuI 510 AGGCCT AGG^CCT
StyI 1104 CCAAGG C^C[AT][AT]GG
XhoI 367 CTCGAG C^TCGAG
XhoII 173 AGATCC [GA]^GATC[CT]
XmaCI 1112 CCCGGG C^CCGGG
XmaI 1112 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AfaI 342 GTAC GT^AC
520 GTAC
Alw44I 43 GTGCAC G^TGCAC
1055 GTGCAC
Aor51HI 681 AGCGCT AGC^GCT
802 AGCGCT
ApaLI 43 GTGCAC G^TGCAC
1055 GTGCAC
Asp700I 562 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
984 GAATCCCTTC
AspI 250 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1065 GACCCTGTC
BanII 1023 GGGCTC G[GA]GC[CT]^C
1109 GGGCCC
BbeI 322 GGCGCC GGCGC^C
933 GGCGCC
BbvCI 121 CCTCAGC CCTCAGC(-5/-2)
765 CCTCAGC
BglI 574 GCCTTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
724 GCCGCACTGGC
BsmBI 48 CGTCTC CGTCTC(1/5)
703 GAGACG
BsrFI 181 GCCGGC [GA]^CCGG[CT]
385 ACCGGC
BssHII 6 GCGCGC G^CGCGC
783 GCGCGC
Cfr10I 181 GCCGGC [GA]^CCGG[CT]
385 ACCGGC
DpnII 174 GATC ^GATC
907 GATC
DraII 371 AGGACCT [GA]G^G[ACGT]CC[CT]
1108 GGGGCCC
Eco47III 681 AGCGCT AGC^GCT
802 AGCGCT
EcoO109I 371 AGGACCT [GA]G^G[ACGT]CC[CT]
1108 GGGGCCC
FauI 234 CCCGC CCCGC(4/6)
347 CCCGC
HinfI 675 GAATC G^A[ACGT]TC
984 GAATC
KasI 322 GGCGCC G^GCGCC
933 GGCGCC
MboI 174 GATC ^GATC
907 GATC
MslI 693 CACAGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
711 CACAGGCGTG
NarI 322 GGCGCC GG^CGCC
933 GGCGCC
NdeII 174 GATC ^GATC
907 GATC
PflFI 250 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1065 GACCCTGTC
PstI 158 CTGCAG CTGCA^G
490 CTGCAG
RsaI 342 GTAC GT^AC
520 GTAC
Sau3AI 174 GATC ^GATC
907 GATC
SfaNI 220 GATGC GCATC(5/9)
812 GATGC
SfcI 158 CTGCAG C^T[GA][CT]AG
490 CTGCAG
SfoI 322 GGCGCC GGC^GCC
933 GGCGCC
TfiI 675 GAATC G^A[AT]TC
984 GAATC
Tth111I 250 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1065 GACCCTGTC
XmnI 562 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
984 GAATCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaI AvaII BanI BbvI BcnI
BfaI BmyI BsaHI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI EcoRII
Fnu4HI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII ItaI
MaeI MaeII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV Sau96I ScrFI SmlI TaqI
TseI TspRI TthHB8I