Restriction map for pFN21AE0478
 (Linear) (Six-base) MAPSORT of: KIEE0478.seq  Check: 9840  from: 1  to: 1125



KIEE0478.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     730    1125 
   Size:        730     395
 
AfeI AGC'GCT

Cuts at:      0     683     804    1125 
   Size:        683     121     321

  Fragments arranged by size: 

                 683     321     121
 
AflIII A'CryG_T

Cuts at:      0     452    1125 
   Size:        452     673
 
AleI CACnn'nnGTG

Cuts at:      0     715    1125 
   Size:        715     410
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      68    1125 
   Size:         68    1057
 
AlwNI CAG_nnn'CTG

Cuts at:      0     315    1125 
   Size:        315     810
 
ApaI G_GGCC'C

Cuts at:      0    1113    1125 
   Size:       1113      12
 
ApaLI G'TGCA_C

Cuts at:      0      43    1055    1125 
   Size:         43    1012      70

  Fragments arranged by size: 

                1012      70      43
 
AvaI C'yCGr_G

Cuts at:      0     367     955    1112    1125 
   Size:        367     588     157      13

  Fragments arranged by size: 

                 588     367     157      13
 
BanI G'GyrC_C

Cuts at:      0     322     388     870     933    1125 
   Size:        322      66     482      63     192

  Fragments arranged by size: 

                 482     322     192      66      63
 
BanII G_rGCy'C

Cuts at:      0    1027    1113    1125 
   Size:       1027      86      12

  Fragments arranged by size: 

                1027      86      12
 
BbeI G_GCGC'C

Cuts at:      0     326     937    1125 
   Size:        326     611     188

  Fragments arranged by size: 

                 611     326     188
 
BbvCI CC'TCA_GC

Cuts at:      0     122     766    1125 
   Size:        122     644     359

  Fragments arranged by size: 

                 644     359     122
 
BfrBI ATG'CAT

Cuts at:      0     815    1125 
   Size:        815     310
 
BglI GCCn_nnn'nGGC

Cuts at:      0     580     730    1125 
   Size:        580     150     395

  Fragments arranged by size: 

                 580     395     150
 
BlpI GC'TnA_GC

Cuts at:      0     308    1125 
   Size:        308     817
 
Bme1580I G_kGCm'C

Cuts at:      0      47    1059    1113    1125 
   Size:         47    1012      54      12

  Fragments arranged by size: 

                1012      54      47      12
 
BmgBI CAC'GTC

Cuts at:      0      48    1125 
   Size:         48    1077
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     628    1125 
   Size:        628     497
 
BmtI G_CTAG'C

Cuts at:      0     340    1125 
   Size:        340     785
 
Bpu10I CC'TnA_GC

Cuts at:      0     122     766    1125 
   Size:        122     644     359

  Fragments arranged by size: 

                 644     359     122
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     487     499    1125 
   Size:        337     150      12     626

  Fragments arranged by size: 

                 626     337     150      12
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     193    1125 
   Size:        193     932
 
BsaHI Gr'CG_yC

Cuts at:      0     227     323     706     934    1125 
   Size:        227      96     383     228     191

  Fragments arranged by size: 

                 383     228     227     191      96
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      25     381     411    1125 
   Size:         25     356      30     714

  Fragments arranged by size: 

                 714     356      30      25
 
BseYI C'CCAG_C

Cuts at:      0     856    1047    1125 
   Size:        856     191      78

  Fragments arranged by size: 

                 856     191      78
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     766    1125 
   Size:        766     359
 
BsiHKAI G_wGCw'C

Cuts at:      0      47     905    1059    1125 
   Size:         47     858     154      66

  Fragments arranged by size: 

                 858     154      66      47
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      54     697    1125 
   Size:         54     643     428

  Fragments arranged by size: 

                 643     428      54
 
Bsp1286I G_dGCh'C

Cuts at:      0      47     905    1027    1059    1113    1125 
   Size:         47     858     122      32      54      12

  Fragments arranged by size: 

                 858     122      54      47      32      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     730    1125 
   Size:        730     395
 
BsrDI GCAATG_nn'

Cuts at:      0     527    1125 
   Size:        527     598
 
BsrFI r'CCGG_y

Cuts at:      0     181     385    1125 
   Size:        181     204     740

  Fragments arranged by size: 

                 740     204     181
 
BssHII G'CGCG_C

Cuts at:      0       6     783    1125 
   Size:          6     777     342

  Fragments arranged by size: 

                 777     342       6
 
BssSI C'ACGA_G

Cuts at:      0    1018    1125 
   Size:       1018     107
 
BstYI r'GATC_y

Cuts at:      0     173    1125 
   Size:        173     952
 
BtgI C'CryG_G

Cuts at:      0     235    1125 
   Size:        235     890
 
BtsI GCAGTG_nn'

Cuts at:      0     502    1125 
   Size:        502     623
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     405     440    1125 
   Size:        405      35     685

  Fragments arranged by size: 

                 685     405      35
 
EaeI y'GGCC_r

Cuts at:      0     665    1125 
   Size:        665     460
 
EcoO109I rG'GnC_Cy

Cuts at:      0     372    1109    1125 
   Size:        372     737      16

  Fragments arranged by size: 

                 737     372      16
 
FspI TGC'GCA

Cuts at:      0     688    1125 
   Size:        688     437
 
HaeII r_GCGC'y

Cuts at:      0     326     685     806     830     937    1125 
   Size:        326     359     121      24     107     188

  Fragments arranged by size: 

                 359     326     188     121     107      24
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      27     627     659    1125 
   Size:         27     600      32     466

  Fragments arranged by size: 

                 600     466      32      27
 
KasI G'GCGC_C

Cuts at:      0     322     933    1125 
   Size:        322     611     192

  Fragments arranged by size: 

                 611     322     192
 
MslI CAynn'nnrTG

Cuts at:      0     697     715    1125 
   Size:        697      18     410

  Fragments arranged by size: 

                 697     410      18
 
MspA1I CmG'CkG

Cuts at:      0      39      98     237     402     914    1125 
   Size:         39      59     139     165     512     211

  Fragments arranged by size: 

                 512     211     165     139      59      39
 
NaeI GCC'GGC

Cuts at:      0     183    1125 
   Size:        183     942
 
NarI GG'CG_CC

Cuts at:      0     323     934    1125 
   Size:        323     611     191

  Fragments arranged by size: 

                 611     323     191
 
NgoMIV G'CCGG_C

Cuts at:      0     181    1125 
   Size:        181     944
 
NheI G'CTAG_C

Cuts at:      0     336    1125 
   Size:        336     789
 
NsiI A_TGCA'T

Cuts at:      0     817    1125 
   Size:        817     308
 
NspI r_CATG'y

Cuts at:      0     819    1125 
   Size:        819     306
 
PfoI T'CCnGG_A

Cuts at:      0     612    1125 
   Size:        612     513
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     350     382    1125 
   Size:        350      32     743

  Fragments arranged by size: 

                 743     350      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     372    1125 
   Size:        372     753
 
PspOMI G'GGCC_C

Cuts at:      0    1109    1125 
   Size:       1109      16
 
PspXI vC'TCGA_Gb

Cuts at:      0     367    1125 
   Size:        367     758
 
PstI C_TGCA'G

Cuts at:      0     162     494    1125 
   Size:        162     332     631

  Fragments arranged by size: 

                 631     332     162
 
PvuII CAG'CTG

Cuts at:      0     914    1125 
   Size:        914     211
 
SacII CC_GC'GG

Cuts at:      0     238    1125 
   Size:        238     887
 
SfcI C'TryA_G

Cuts at:      0     158     490    1125 
   Size:        158     332     635

  Fragments arranged by size: 

                 635     332     158
 
SfoI GGC'GCC

Cuts at:      0     324     935    1125 
   Size:        324     611     190

  Fragments arranged by size: 

                 611     324     190
 
SmaI CCC'GGG

Cuts at:      0    1114    1125 
   Size:       1114      11
 
SmlI C'TyrA_G

Cuts at:      0     352     367     502     514    1125 
   Size:        352      15     135      12     611

  Fragments arranged by size: 

                 611     352     135      15      12
 
StuI AGG'CCT

Cuts at:      0     512    1125 
   Size:        512     613
 
StyI C'CwwG_G

Cuts at:      0    1104    1125 
   Size:       1104      21
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     381     413    1079    1111    1125 
   Size:        381      32     666      32      14

  Fragments arranged by size: 

                 666     381      32      32      14
 
Tth111I GACn'n_nGTC

Cuts at:      0     253    1068    1125 
   Size:        253     815      57

  Fragments arranged by size: 

                 815     253      57
 
XhoI C'TCGA_G

Cuts at:      0     367    1125 
   Size:        367     758
 
XmaI C'CCGG_G

Cuts at:      0    1112    1125 
   Size:       1112      13
 
XmnI GAAnn'nnTTC

Cuts at:      0     566     988    1125 
   Size:        566     422     137

  Fragments arranged by size: 

                 566     422     137
 

 Enzymes that do cut:

     AarI     AfeI   AflIII     AleI     AlfI    AlwNI     ApaI    ApaLI
     AvaI     BanI    BanII     BbeI    BbvCI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaHI
    BsaXI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrDI
    BsrFI   BssHII    BssSI    BstYI     BtgI     BtsI    CspCI     EaeI
 EcoO109I     FspI    HaeII    Hin4I     KasI     MslI   MspA1I     NaeI
     NarI   NgoMIV     NheI     NsiI     NspI     PfoI     PpiI    PpuMI
   PspOMI    PspXI     PstI    PvuII    SacII     SfcI     SfoI     SmaI
     SmlI     StuI     StyI     TstI  Tth111I     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI     AhdI
     AloI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbsI     BcgI    BciVI     BclI    BglII     BplI     BpmI    BsaAI
    BsaBI    BsaWI    BseRI    BsiEI    BsiWI     BsmI    BspEI    BspHI
    BsrBI    BsrGI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     NcoI     NdeI     NotI     NruI     PacI     PasI     PciI    PflMI
     PmeI     PmlI    PshAI     PsiI     PsrI     PvuI    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
     TatI     XbaI     XcmI     ZraI
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