(Circular) (Six-base) MAPSORT of: pFN21AE3694.seq Check: 7976 from: 1 to: 4981 pFN21AE3694 4981 bp With 182 enzymes: SgfI * July 6, 2012 17:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2791 278 Size: 53 83 186 936 1255 2468 Fragments arranged by size: 2468 1255 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2324 1937 Size: 387 4594 Acc65I G'GTAC_C Cuts at: 2302 2302 Size: 4981 AclI AA'CG_TT Cuts at: 1563 2280 3108 3481 1563 Size: 717 828 373 3063 Fragments arranged by size: 3063 828 717 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3044 4428 4696 1632 Size: 1412 1384 268 1917 Fragments arranged by size: 1917 1412 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4784 AflIII A'CryG_T Cuts at: 1203 2259 3880 4857 1203 Size: 1056 1621 977 1327 Fragments arranged by size: 1621 1327 1056 977 AgeI A'CCGG_T Cuts at: 3812 3812 Size: 4981 AhdI GACnn_n'nnGTC Cuts at: 3710 3710 Size: 4981 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4981 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4981 AlwNI CAG_nnn'CTG Cuts at: 2175 2724 4296 2175 Size: 549 1572 2860 Fragments arranged by size: 2860 1572 549 ApaLI G'TGCA_C Cuts at: 3038 4194 3038 Size: 1156 3825 ApoI r'AATT_y Cuts at: 1493 2290 2516 2668 4777 1493 Size: 797 226 152 2109 1697 Fragments arranged by size: 2109 1697 797 226 152 AseI AT'TA_AT Cuts at: 160 1032 3535 160 Size: 872 2503 1606 Fragments arranged by size: 2503 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4981 AvaI C'yCGr_G Cuts at: 1870 1943 2306 1870 Size: 73 363 4545 Fragments arranged by size: 4545 363 73 BamHI G'GATC_C Cuts at: 2311 2678 2311 Size: 367 4614 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2302 3757 618 Size: 325 217 627 515 1455 1842 Fragments arranged by size: 1842 1455 627 515 325 217 BanII G_rGCy'C Cuts at: 1391 2087 2300 1391 Size: 696 213 4072 Fragments arranged by size: 4072 696 213 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4010 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3172 3206 1903 Size: 34 1235 34 3678 Fragments arranged by size: 3678 1235 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2873 4083 2873 Size: 1210 3771 BclI T'GATC_A Cuts at: 1548 1548 Size: 4981 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3592 136 Size: 107 122 71 631 605 1920 1525 Fragments arranged by size: 1920 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4976 4976 Size: 4981 BlpI GC'TnA_GC Cuts at: 2398 2398 Size: 4981 Bme1580I G_kGCm'C Cuts at: 1790 3042 4198 1790 Size: 1252 1156 2573 Fragments arranged by size: 2573 1252 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4981 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2229 2712 3670 Size: 416 53 83 399 24 812 483 958 Cuts at: 3670 442 Size: 1753 Fragments arranged by size: 1753 958 812 483 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4981 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3641 1239 Size: 2402 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3106 3971 4269 3106 Size: 865 298 3818 Fragments arranged by size: 3818 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2156 3644 882 Size: 617 657 1488 2219 Fragments arranged by size: 2219 1488 657 617 BsaAI yAC'GTr Cuts at: 493 1204 2260 2772 493 Size: 711 1056 512 2702 Fragments arranged by size: 2702 1056 711 512 BsaBI GATnn'nnATC Cuts at: 4975 4975 Size: 4981 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2788 3170 3805 275 Size: 53 83 186 936 1255 382 635 1451 Fragments arranged by size: 1451 1255 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3414 3812 4086 4233 4644 3414 Size: 398 274 147 411 3751 Fragments arranged by size: 3751 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2246 2276 1178 Size: 30 165 30 843 30 3883 Fragments arranged by size: 3883 843 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 1186 Size: 838 4143 BseYI C'CCAG_C Cuts at: 2181 4184 4680 2181 Size: 2003 496 2482 Fragments arranged by size: 2482 2003 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4408 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2431 3192 4220 1540 Size: 275 186 430 761 1028 2301 Fragments arranged by size: 2301 1028 761 430 275 186 BsiHKAI G_wGCw'C Cuts at: 3042 3127 4198 3042 Size: 85 1071 3825 Fragments arranged by size: 3825 1071 85 BsmI GAATG_Cn' Cuts at: 2492 2585 2492 Size: 93 4888 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2300 3042 3127 4198 1391 Size: 399 297 213 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 297 213 85 BspEI T'CCGG_A Cuts at: 4644 4644 Size: 4981 BspHI T'CATG_A Cuts at: 2870 4600 2870 Size: 1730 3251 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2336 844 Size: 1492 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2868 1394 Size: 429 1045 3507 Fragments arranged by size: 3507 1045 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3476 3650 65 Size: 1183 2228 174 1396 Fragments arranged by size: 2228 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3625 3812 1700 Size: 110 1815 187 2869 Fragments arranged by size: 2869 1815 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4981 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4981 BssSI C'ACGA_G Cuts at: 2036 3041 4053 2036 Size: 1005 1012 2964 Fragments arranged by size: 2964 1012 1005 BstAPI GCAn_nnn'nTGC Cuts at: 794 2175 794 Size: 1381 3600 BstBI TT'CG_AA Cuts at: 3785 3785 Size: 4981 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4981 BstYI r'GATC_y Cuts at: 1972 2311 2678 3063 3080 4532 4618 4630 4976 Size: 339 367 385 17 1452 86 12 346 Cuts at: 4976 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 339 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4429 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4242 Fragments arranged by size: 4242 683 56 BtsI GCAGTG_nn' Cuts at: 2497 3291 3311 2497 Size: 794 20 4167 Fragments arranged by size: 4167 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4981 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4946 DraI TTT'AAA Cuts at: 2285 2285 Size: 4981 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2026 3988 809 Size: 507 710 1962 1802 Fragments arranged by size: 1962 1802 710 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2428 3317 4946 Size: 54 1425 50 275 18 598 889 1629 Cuts at: 4946 8 Size: 43 Fragments arranged by size: 1629 1425 889 598 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2428 1537 Size: 275 616 4090 Fragments arranged by size: 4090 616 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2911 1353 Size: 1558 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3568 3942 4088 387 Size: 1064 138 1979 374 146 1280 Fragments arranged by size: 1979 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3044 3641 4428 4696 1239 Size: 393 1412 597 787 268 1524 Fragments arranged by size: 1524 1412 787 597 393 268 EcoRI G'AATT_C Cuts at: 2290 2668 2290 Size: 378 4603 FspI TGC'GCA Cuts at: 3487 3487 Size: 4981 HaeII r_GCGC'y Cuts at: 1678 4128 1678 Size: 2450 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3622 3654 3696 3728 1373 Size: 32 135 32 2050 32 42 32 2626 Fragments arranged by size: 2626 2050 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2325 2571 669 Size: 988 281 387 246 3079 Fragments arranged by size: 3079 988 387 281 246 HindIII A'AGCT_T Cuts at: 748 2129 748 Size: 1381 3600 HpaI GTT'AAC Cuts at: 2571 2571 Size: 4981 KpnI G_GTAC'C Cuts at: 2306 2306 Size: 4981 MfeI C'AATT_G Cuts at: 2580 2580 Size: 4981 MluI A'CGCG_T Cuts at: 4857 4857 Size: 4981 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4095 4279 1305 Size: 2790 184 2007 Fragments arranged by size: 2790 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3159 Fragments arranged by size: 3159 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2939 3298 3457 518 Size: 648 1773 359 159 2042 Fragments arranged by size: 2042 1773 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2189 2397 3074 4222 4467 1606 Size: 583 208 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 583 245 208 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4429 NdeI CA'TA_TG Cuts at: 387 387 Size: 4981 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4981 NotI GC'GGCC_GC Cuts at: 2428 2428 Size: 4981 NspI r_CATG'y Cuts at: 1132 1229 2339 3884 1132 Size: 97 1110 1545 2229 Fragments arranged by size: 2229 1545 1110 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4981 PciI A'CATG_T Cuts at: 3880 3880 Size: 4981 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4981 PfoI T'CCnGG_A Cuts at: 2250 2250 Size: 4981 PmeI GTTT'AAAC Cuts at: 2285 2285 Size: 4981 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3050 3082 4563 4595 3050 Size: 32 1481 32 3436 Fragments arranged by size: 3436 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4981 PsiI TTA'TAA Cuts at: 2551 2551 Size: 4981 PstI C_TGCA'G Cuts at: 830 2333 830 Size: 1503 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4981 PvuII CAG'CTG Cuts at: 2189 2189 Size: 4981 SalI G'TCGA_C Cuts at: 1936 2323 1936 Size: 387 4594 SbfI CC_TGCA'GG Cuts at: 2333 2333 Size: 4981 ScaI AGT'ACT Cuts at: 3229 4941 3229 Size: 1712 3269 SfcI C'TryA_G Cuts at: 826 1045 2329 3464 4145 4336 826 Size: 219 1284 1135 681 191 1471 Fragments arranged by size: 1471 1284 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4981 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4981 SmaI CCC'GGG Cuts at: 1872 2308 1872 Size: 436 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3085 3986 4248 820 Size: 197 926 1142 901 262 1553 Fragments arranged by size: 1553 1142 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2772 493 Size: 2279 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4981 SphI G_CATG'C Cuts at: 1132 2339 1132 Size: 1207 3774 SspI AAT'ATT Cuts at: 5 52 2905 5 Size: 47 2853 2081 Fragments arranged by size: 2853 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4981 StyI C'CwwG_G Cuts at: 513 1065 2420 513 Size: 552 1355 3074 Fragments arranged by size: 3074 1355 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3008 3025 3178 3363 3808 325 Size: 1255 1428 17 153 185 445 1498 Fragments arranged by size: 1498 1428 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3227 4939 96 Size: 274 80 33 51 1443 1250 1712 138 Fragments arranged by size: 1712 1443 1250 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4941 4973 4941 Size: 32 4949 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4981 XbaI T'CTAG_A Cuts at: 2317 2317 Size: 4981 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4981 XmaI C'CCGG_G Cuts at: 1870 2306 1870 Size: 436 4545 XmnI GAAnn'nnTTC Cuts at: 3110 3110 Size: 4981 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2789 276 Size: 53 83 186 936 1255 2468 Fragments arranged by size: 2468 1255 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI