Restriction map for
pFN21AB6737
(Circular) (Six-base) MAPSORT of: pFN21AB6737.seq Check: 1686 from: 1 to: 4996
pFN21AB6737 4996 bp
With 182 enzymes: SgfI *
October 20, 2009 10:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2105 2105
Size: 4996
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2806 278
Size: 53 83 186 936 1270 2468
Fragments arranged by size:
2468 1270 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2339 1937
Size: 402 4594
Acc65I G'GTAC_C
Cuts at: 2317 2317
Size: 4996
AclI AA'CG_TT
Cuts at: 1563 3123 3496 1563
Size: 1560 373 3063
Fragments arranged by size:
3063 1560 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2038 2117 3059 4443 4711 1632
Size: 406 79 942 1384 268 1917
Fragments arranged by size:
1917 1384 942 406 268 79
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4799
AflIII A'CryG_T
Cuts at: 1203 3895 4872 1203
Size: 2692 977 1327
Fragments arranged by size:
2692 1327 977
AgeI A'CCGG_T
Cuts at: 3827 3827
Size: 4996
AhdI GACnn_n'nnGTC
Cuts at: 3725 3725
Size: 4996
AleI CACnn'nnGTG
Cuts at: 1166 2026 1166
Size: 860 4136
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4996
AlwNI CAG_nnn'CTG
Cuts at: 2084 2739 4311 2084
Size: 655 1572 2769
Fragments arranged by size:
2769 1572 655
ApaLI G'TGCA_C
Cuts at: 3053 4209 3053
Size: 1156 3840
ApoI r'AATT_y
Cuts at: 1493 2305 2531 2683 4792 1493
Size: 812 226 152 2109 1697
Fragments arranged by size:
2109 1697 812 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3550 160
Size: 872 2518 1606
Fragments arranged by size:
2518 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4996
AvaI C'yCGr_G
Cuts at: 1870 1943 2321 1870
Size: 73 378 4545
Fragments arranged by size:
4545 378 73
BamHI G'GATC_C
Cuts at: 2326 2693 2326
Size: 367 4629
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2317 3772 618
Size: 325 217 627 530 1455 1842
Fragments arranged by size:
1842 1455 627 530 325 217
BanII G_rGCy'C
Cuts at: 1391 2070 2315 1391
Size: 679 245 4072
Fragments arranged by size:
4072 679 245
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4025
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3187 3221 1903
Size: 34 1250 34 3678
Fragments arranged by size:
3678 1250 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2888 4098 2888
Size: 1210 3786
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4996
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3607 136
Size: 107 122 71 631 605 1935 1525
Fragments arranged by size:
1935 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4991 4991
Size: 4996
BlpI GC'TnA_GC
Cuts at: 2413 2413
Size: 4996
Bme1580I G_kGCm'C
Cuts at: 1790 3057 4213 1790
Size: 1267 1156 2573
Fragments arranged by size:
2573 1267 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4996
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2727 3685 442
Size: 416 53 83 399 24 1310 958 1753
Fragments arranged by size:
1753 1310 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4996
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3656 1239
Size: 2417 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3121 3986 4284 3121
Size: 865 298 3833
Fragments arranged by size:
3833 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2017 3659 882
Size: 617 518 1642 2219
Fragments arranged by size:
2219 1642 617 518
BsaAI yAC'GTr
Cuts at: 493 1204 2787 493
Size: 711 1583 2702
Fragments arranged by size:
2702 1583 711
BsaBI GATnn'nnATC
Cuts at: 4990 4990
Size: 4996
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2803 3185 3820 275
Size: 53 83 186 936 1270 382 635 1451
Fragments arranged by size:
1451 1270 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3429 3827 4101 4248 4659 3429
Size: 398 274 147 411 3766
Fragments arranged by size:
3766 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4771
Fragments arranged by size:
4771 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4996
BseYI C'CCAG_C
Cuts at: 2072 4199 4695 2072
Size: 2127 496 2373
Fragments arranged by size:
2373 2127 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2267 1163
Size: 573 531 3892
Fragments arranged by size:
3892 573 531
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2446 3207 4235 1540
Size: 275 186 445 761 1028 2301
Fragments arranged by size:
2301 1028 761 445 275 186
BsiHKAI G_wGCw'C
Cuts at: 3057 3142 4213 3057
Size: 85 1071 3840
Fragments arranged by size:
3840 1071 85
BsmI GAATG_Cn'
Cuts at: 2507 2600 2507
Size: 93 4903
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2315 3057 3142 4213 1391
Size: 399 280 245 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 280 245 85
BspEI T'CCGG_A
Cuts at: 4659 4659
Size: 4996
BspHI T'CATG_A
Cuts at: 2885 4615 2885
Size: 1730 3266
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2105 2351 844
Size: 1261 246 3489
Fragments arranged by size:
3489 1261 246
BsrBI CCG'CTC
Cuts at: 1394 1823 2883 1394
Size: 429 1060 3507
Fragments arranged by size:
3507 1060 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3491 3665 65
Size: 1183 2243 174 1396
Fragments arranged by size:
2243 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3640 3827 1700
Size: 110 1830 187 2869
Fragments arranged by size:
2869 1830 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4996
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4996
BssSI C'ACGA_G
Cuts at: 3056 4068 3056
Size: 1012 3984
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4996
BstBI TT'CG_AA
Cuts at: 3800 3800
Size: 4996
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4996
BstYI r'GATC_y
Cuts at: 1972 2326 2693 3078 3095 4547 4633 4645 4991
Size: 354 367 385 17 1452 86 12 346
Cuts at: 4991 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 354 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4444
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4257
Fragments arranged by size:
4257 683 56
BtsI GCAGTG_nn'
Cuts at: 2512 3306 3326 2512
Size: 794 20 4182
Fragments arranged by size:
4182 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4996
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4961
DraI TTT'AAA
Cuts at: 2300 2300
Size: 4996
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4003 809
Size: 507 2687 1802
Fragments arranged by size:
2687 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2112 2208 2443
Size: 54 1425 50 275 18 282 96 235
Cuts at: 2443 3332 4961 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 282 275 235 96 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2443 1537
Size: 275 631 4090
Fragments arranged by size:
4090 631 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2926 1353
Size: 1573 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3583 3957 4103 387
Size: 1064 138 1994 374 146 1280
Fragments arranged by size:
1994 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2038 2117 3059 3656 4443 4711 1239
Size: 393 406 79 942 597 787 268 1524
Fragments arranged by size:
1524 942 787 597 406 393 268 79
EcoRI G'AATT_C
Cuts at: 2305 2683 2305
Size: 378 4618
EcoRV GAT'ATC
Cuts at: 2218 2218
Size: 4996
FspI TGC'GCA
Cuts at: 3502 3502
Size: 4996
HaeII r_GCGC'y
Cuts at: 1678 4143 1678
Size: 2465 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2261 2293 3637 3669 3711
Size: 32 135 32 689 32 1344 32 42
Cuts at: 3711 3743 1373
Size: 32 2626
Fragments arranged by size:
2626 1344 689 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2340 2586 669
Size: 988 281 402 246 3079
Fragments arranged by size:
3079 988 402 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4996
HpaI GTT'AAC
Cuts at: 2586 2586
Size: 4996
KpnI G_GTAC'C
Cuts at: 2321 2321
Size: 4996
MfeI C'AATT_G
Cuts at: 2595 2595
Size: 4996
MluI A'CGCG_T
Cuts at: 4872 4872
Size: 4996
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2306 4110 4294 1305
Size: 1001 1804 184 2007
Fragments arranged by size:
2007 1804 1001 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2114 2210 10
Size: 54 1425 343 282 96 2796
Fragments arranged by size:
2796 1425 343 282 96 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2026 2954 3313 3472 518
Size: 648 860 928 359 159 2042
Fragments arranged by size:
2042 928 860 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2048 2412 3089 4237 4482 1606
Size: 442 364 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 442 364 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4444
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4996
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4996
NotI GC'GGCC_GC
Cuts at: 2443 2443
Size: 4996
NspI r_CATG'y
Cuts at: 1132 1229 2354 3899 1132
Size: 97 1125 1545 2229
Fragments arranged by size:
2229 1545 1125 97
PasI CC'CwG_GG
Cuts at: 1348 2062 1348
Size: 714 4282
PciI A'CATG_T
Cuts at: 3895 3895
Size: 4996
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4996
PmeI GTTT'AAAC
Cuts at: 2300 2300
Size: 4996
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3065 3097 4578 4610 3065
Size: 32 1481 32 3451
Fragments arranged by size:
3451 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4996
PsiI TTA'TAA
Cuts at: 2566 2566
Size: 4996
PstI C_TGCA'G
Cuts at: 830 2348 830
Size: 1518 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4996
PvuII CAG'CTG
Cuts at: 2048 2048
Size: 4996
SalI G'TCGA_C
Cuts at: 1936 2338 1936
Size: 402 4594
SbfI CC_TGCA'GG
Cuts at: 2348 2348
Size: 4996
ScaI AGT'ACT
Cuts at: 3244 4956 3244
Size: 1712 3284
SfcI C'TryA_G
Cuts at: 826 1045 2149 2344 3479 4160 4351 826
Size: 219 1104 195 1135 681 191 1471
Fragments arranged by size:
1471 1135 1104 681 219 195 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4996
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4996
SmaI CCC'GGG
Cuts at: 1872 2323 1872
Size: 451 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3100 4001 4263 820
Size: 197 926 1157 901 262 1553
Fragments arranged by size:
1553 1157 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2787 493
Size: 2294 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4996
SphI G_CATG'C
Cuts at: 1132 2354 1132
Size: 1222 3774
SspI AAT'ATT
Cuts at: 5 52 2920 5
Size: 47 2868 2081
Fragments arranged by size:
2868 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4996
StyI C'CwwG_G
Cuts at: 513 1065 2211 2435 513
Size: 552 1146 224 3074
Fragments arranged by size:
3074 1146 552 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2233 3023 3040 3193 3378 3823 325
Size: 1255 653 790 17 153 185 445 1498
Fragments arranged by size:
1498 1255 790 653 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3242 4954 96
Size: 274 80 33 51 1443 1265 1712 138
Fragments arranged by size:
1712 1443 1265 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4956 4988 4956
Size: 32 4964
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4996
XbaI T'CTAG_A
Cuts at: 2332 2332
Size: 4996
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2069 2263 2069
Size: 194 4802
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4996
XmaI C'CCGG_G
Cuts at: 1870 2321 1870
Size: 451 4545
XmnI GAAnn'nnTTC
Cuts at: 3125 3125
Size: 4996
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2804 276
Size: 53 83 186 936 1270 2468
Fragments arranged by size:
2468 1270 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI