################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbvI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BseAI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HgaI HincII HindII HindIII HpaI HphI ItaI KpnI KspI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI MvnI MwoI NcoI NdeI NdeII NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI Tru9I TseI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcyI 307 GGCGCC G[GA]^CG[CT]C AfaI 177 GTAC GT^AC AvaI 140 CCCGGG C^[CT]CG[GA]G AvaII 372 GGTCC G^G[AT]CC BanI 307 GGCGCC G^G[CT][GA]CC BanII 390 GAGCTC G[GA]GC[CT]^C BbeI 307 GGCGCC GGCGC^C BbsI 193 GAAGAC GAAGAC(2/6) BbvCI 105 GCTGAGG CCTCAGC(-5/-2) BfaI 160 CTAG C^TAG BpmI 321 CTCCAG CTGGAG(16/14) BpuAI 193 GAAGAC GAAGAC(2/6) BsaHI 307 GGCGCC G[GA]^CG[CT]C BsaWI 44 ACCGGA [AT]^CCGG[AT] BseRI 100 GAGGAG GAGGAG(10/8) BsmAI 238 GAGAC GTCTC(1/5) BsmBI 238 GAGACG CGTCTC(1/5) BsoBI 140 CCCGGG C^[CT]CG[GA]G BssSI 85 CACGAG CACGAG(-5/-1) BstF5I 189 GGATG GGATG(2/0) Cac8I 9 GCCGGC GC[ACGT]^[ACGT]GC DraII 371 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoNI 340 CCTTCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 371 GGGTCCC [GA]G^G[ACGT]CC[CT] FokI 189 GGATG GGATG(9/13) HaeII 307 GGCGCC [GA]GCGC^[CT] Hin1I 307 GGCGCC G[GA]^CG[CT]C KasI 307 GGCGCC G^GCGCC MaeI 160 CTAG C^TAG MaeII 185 ACGT A^CGT MspA1I 298 CCGCTG C[AC]G^C[GT]G NaeI 9 GCCGGC GCC^GGC NarI 307 GGCGCC GG^CGCC NgoMIV 9 GCCGGC G^CCGGC PpuMI 371 GGGTCCC [GA]G^G[AT]CC[CT] PstI 172 CTGCAG CTGCA^G RsaI 177 GTAC GT^AC SacI 390 GAGCTC GAGCT^C SfcI 172 CTGCAG C^T[GA][CT]AG SfoI 307 GGCGCC GGC^GCC SmaI 140 CCCGGG CCC^GGG SmlI 64 CTCAAG C^T[CT][GA]AG StuI 362 AGGCCT AGG^CCT TfiI 48 GAATC G^A[AT]TC Tsp509I 125 AATT ^AATT TspRI 216 CACTG CA[GC]TG[ACGT][ACGT]^ XmaCI 140 CCCGGG C^CCGGG XmaI 140 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AspHI 82 GAGCAC G[AT]GC[AT]^C 390 GAGCTC BmyI 82 GAGCAC G[AGT]GC[ACT]^C 390 GAGCTC BsaJI 140 CCCGGG C^C[ACGT][ACGT]GG 368 CCGGGG BsiHKAI 82 GAGCAC G[AT]GC[AT]^C 390 GAGCTC Bsp1286I 82 GAGCAC G[AGT]GC[ACT]^C 390 GAGCTC BsrFI 9 GCCGGC [GA]^CCGG[CT] 270 ACCGGC BstNI 96 CCAGG CC^[AT]GG 405 CCAGG CfoI 115 GCGC GCG^C 308 GCGC Cfr10I 9 GCCGGC [GA]^CCGG[CT] 270 ACCGGC EarI 198 CTCTTC CTCTTC(1/4) 285 GAAGAG EcoRII 96 CCAGG ^CC[AT]GG 405 CCAGG HhaI 115 GCGC GCG^C 308 GCGC HinP1I 115 GCGC G^CGC 308 GCGC Ksp632I 198 CTCTTC CTCTTC(1/4) 285 GAAGAG MvaI 96 CCAGG CC^[AT]GG 405 CCAGG PleI 196 GACTC GAGTC(4/5) 205 GACTC SfaNI 211 GATGC GCATC(5/9) 220 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AciI AluI BcnI BsiYI BslI BsmFI BssKI Cfr13I DdeI HaeIII HapII HinfI HpaII MboII MnlI MspI NciI NlaIV Sau96I ScrFI