(Circular) (Six-base) MAPSORT of: pFN21AE1103.seq Check: 5638 from: 1 to: 5116 pFN21AE1103 5116 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2926 278 Size: 53 83 186 936 1390 2468 Fragments arranged by size: 2468 1390 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2459 1937 Size: 522 4594 Acc65I G'GTAC_C Cuts at: 2437 2437 Size: 5116 AclI AA'CG_TT Cuts at: 1563 3243 3616 1563 Size: 1680 373 3063 Fragments arranged by size: 3063 1680 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2078 2156 2348 3179 4563 4831 1632 Size: 446 78 192 831 1384 268 1917 Fragments arranged by size: 1917 1384 831 446 268 192 78 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4919 AflIII A'CryG_T Cuts at: 1203 4015 4992 1203 Size: 2812 977 1327 Fragments arranged by size: 2812 1327 977 AgeI A'CCGG_T Cuts at: 3947 3947 Size: 5116 AhdI GACnn_n'nnGTC Cuts at: 3845 3845 Size: 5116 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5116 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5116 AlwNI CAG_nnn'CTG Cuts at: 2859 4431 2859 Size: 1572 3544 ApaLI G'TGCA_C Cuts at: 3173 4329 3173 Size: 1156 3960 ApoI r'AATT_y Cuts at: 1493 2425 2651 2803 4912 1493 Size: 932 226 152 2109 1697 Fragments arranged by size: 2109 1697 932 226 152 AseI AT'TA_AT Cuts at: 160 1032 3670 160 Size: 872 2638 1606 Fragments arranged by size: 2638 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5116 AvaI C'yCGr_G Cuts at: 1870 1943 2145 2441 1870 Size: 73 202 296 4545 Fragments arranged by size: 4545 296 202 73 BamHI G'GATC_C Cuts at: 2446 2813 2446 Size: 367 4749 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2312 2437 3892 618 Size: 325 217 627 525 125 1455 1842 Fragments arranged by size: 1842 1455 627 525 325 217 125 BanII G_rGCy'C Cuts at: 1391 2399 2435 1391 Size: 1008 36 4072 Fragments arranged by size: 4072 1008 36 BbeI G_GCGC'C Cuts at: 2316 2316 Size: 5116 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2205 928 Size: 971 306 3839 Fragments arranged by size: 3839 971 306 BbvCI CC'TCA_GC Cuts at: 2111 2111 Size: 5116 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3307 3341 1903 Size: 34 1370 34 3678 Fragments arranged by size: 3678 1370 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3008 4218 3008 Size: 1210 3906 BclI T'GATC_A Cuts at: 1548 1548 Size: 5116 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3727 136 Size: 107 122 71 631 605 2055 1525 Fragments arranged by size: 2055 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5111 5111 Size: 5116 BlpI GC'TnA_GC Cuts at: 2533 2533 Size: 5116 Bme1580I G_kGCm'C Cuts at: 1790 3177 4333 1790 Size: 1387 1156 2573 Fragments arranged by size: 2573 1387 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5116 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2847 3805 442 Size: 416 53 83 399 24 1430 958 1753 Fragments arranged by size: 1753 1430 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5116 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2316 2316 Size: 5116 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2311 3776 1239 Size: 1072 1465 2579 Fragments arranged by size: 2579 1465 1072 Bpu10I CC'TnA_GC Cuts at: 2111 2393 2111 Size: 282 4834 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2054 3241 4106 4404 2054 Size: 1187 865 298 2766 Fragments arranged by size: 2766 1187 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3779 882 Size: 617 2280 2219 Fragments arranged by size: 2280 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2907 493 Size: 711 1703 2702 Fragments arranged by size: 2702 1703 711 BsaBI GATnn'nnATC Cuts at: 5110 5110 Size: 5116 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2313 2923 3305 3940 Size: 53 83 186 936 780 610 382 635 Cuts at: 3940 275 Size: 1451 Fragments arranged by size: 1451 936 780 635 610 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2049 3549 3947 4221 4368 4779 2049 Size: 1500 398 274 147 411 2386 Fragments arranged by size: 2386 1500 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4891 Fragments arranged by size: 4891 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 1186 Size: 934 4182 BseYI C'CCAG_C Cuts at: 2305 4319 4815 2305 Size: 2014 496 2606 Fragments arranged by size: 2606 2014 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4543 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2566 3327 4355 1540 Size: 275 186 565 761 1028 2301 Fragments arranged by size: 2301 1028 761 565 275 186 BsiHKAI G_wGCw'C Cuts at: 2091 2399 3177 3262 4333 2091 Size: 308 778 85 1071 2874 Fragments arranged by size: 2874 1071 778 308 85 BsmI GAATG_Cn' Cuts at: 2627 2720 2627 Size: 93 5023 BsmBI CGTCTCn'nnnn_ Cuts at: 2237 2237 Size: 5116 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2399 2435 3177 3262 4333 1391 Size: 399 301 308 36 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 308 301 85 36 BspEI T'CCGG_A Cuts at: 4779 4779 Size: 5116 BspHI T'CATG_A Cuts at: 3005 4735 3005 Size: 1730 3386 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2471 844 Size: 1627 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3003 1394 Size: 429 1180 3507 Fragments arranged by size: 3507 1180 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3611 3785 65 Size: 1183 2363 174 1396 Fragments arranged by size: 2363 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2014 2275 3760 3947 1700 Size: 110 204 261 1485 187 2869 Fragments arranged by size: 2869 1485 261 204 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5116 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5116 BssSI C'ACGA_G Cuts at: 2090 3176 4188 2090 Size: 1086 1012 3018 Fragments arranged by size: 3018 1086 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5116 BstBI TT'CG_AA Cuts at: 3920 3920 Size: 5116 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5116 BstYI r'GATC_y Cuts at: 1972 2446 2813 3198 3215 4667 4753 4765 5111 Size: 474 367 385 17 1452 86 12 346 Cuts at: 5111 1972 Size: 1977 Fragments arranged by size: 1977 1452 474 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4564 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4377 Fragments arranged by size: 4377 683 56 BtsI GCAGTG_nn' Cuts at: 2220 2632 3426 3446 2220 Size: 412 794 20 3890 Fragments arranged by size: 3890 794 412 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5116 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5081 DraI TTT'AAA Cuts at: 2420 2420 Size: 5116 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4123 809 Size: 507 2807 1802 Fragments arranged by size: 2807 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2563 3452 5081 Size: 54 1425 50 275 18 733 889 1629 Cuts at: 5081 8 Size: 43 Fragments arranged by size: 1629 1425 889 733 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2563 1537 Size: 275 751 4090 Fragments arranged by size: 4090 751 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2209 2285 3046 1353 Size: 856 76 761 3423 Fragments arranged by size: 3423 856 761 76 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2033 3703 4077 4223 387 Size: 1064 138 444 1670 374 146 1280 Fragments arranged by size: 1670 1280 1064 444 374 146 138 EcoICRI GAG'CTC Cuts at: 2397 2397 Size: 5116 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2156 2311 2348 3179 3776 4563 Size: 393 446 78 155 37 831 597 787 Cuts at: 4563 4831 1239 Size: 268 1524 Fragments arranged by size: 1524 831 787 597 446 393 268 155 78 37 EcoNI CCTnn'n_nnAGG Cuts at: 2349 2349 Size: 5116 EcoO109I rG'GnC_Cy Cuts at: 2377 2377 Size: 5116 EcoRI G'AATT_C Cuts at: 2425 2803 2425 Size: 378 4738 FspI TGC'GCA Cuts at: 3622 3622 Size: 5116 HaeII r_GCGC'y Cuts at: 1678 2316 4263 1678 Size: 638 1947 2531 Fragments arranged by size: 2531 1947 638 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2186 2218 3757 3789 3831 Size: 32 135 32 614 32 1539 32 42 Cuts at: 3831 3863 1373 Size: 32 2626 Fragments arranged by size: 2626 1539 614 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2460 2706 669 Size: 988 281 522 246 3079 Fragments arranged by size: 3079 988 522 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5116 HpaI GTT'AAC Cuts at: 2706 2706 Size: 5116 KasI G'GCGC_C Cuts at: 2312 2312 Size: 5116 KpnI G_GTAC'C Cuts at: 2441 2441 Size: 5116 MfeI C'AATT_G Cuts at: 2715 2715 Size: 5116 MluI A'CGCG_T Cuts at: 4992 4992 Size: 5116 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4230 4414 1305 Size: 2925 184 2007 Fragments arranged by size: 2925 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3294 Fragments arranged by size: 3294 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3074 3433 3592 518 Size: 648 1908 359 159 2042 Fragments arranged by size: 2042 1908 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2305 2532 3209 4357 4602 1606 Size: 699 227 677 1148 245 2120 Fragments arranged by size: 2120 1148 699 677 245 227 NaeI GCC'GGC Cuts at: 2016 2016 Size: 5116 NarI GG'CG_CC Cuts at: 2313 2313 Size: 5116 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4564 NdeI CA'TA_TG Cuts at: 387 387 Size: 5116 NgoMIV G'CCGG_C Cuts at: 2014 2014 Size: 5116 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5116 NotI GC'GGCC_GC Cuts at: 2563 2563 Size: 5116 NspI r_CATG'y Cuts at: 1132 1229 2474 4019 1132 Size: 97 1245 1545 2229 Fragments arranged by size: 2229 1545 1245 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5116 PciI A'CATG_T Cuts at: 4015 4015 Size: 5116 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5116 PfoI T'CCnGG_A Cuts at: 2100 2100 Size: 5116 PmeI GTTT'AAAC Cuts at: 2420 2420 Size: 5116 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3185 3217 4698 4730 3185 Size: 32 1481 32 3571 Fragments arranged by size: 3571 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2377 2377 Size: 5116 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5116 PsiI TTA'TAA Cuts at: 2686 2686 Size: 5116 PstI C_TGCA'G Cuts at: 830 2181 2468 830 Size: 1351 287 3478 Fragments arranged by size: 3478 1351 287 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5116 SacI G_AGCT'C Cuts at: 2399 2399 Size: 5116 SalI G'TCGA_C Cuts at: 1936 2458 1936 Size: 522 4594 SanDI GG'GwC_CC Cuts at: 2377 2377 Size: 5116 SbfI CC_TGCA'GG Cuts at: 2468 2468 Size: 5116 ScaI AGT'ACT Cuts at: 3364 5076 3364 Size: 1712 3404 SfcI C'TryA_G Cuts at: 826 1045 2177 2464 3599 4280 4471 826 Size: 219 1132 287 1135 681 191 1471 Fragments arranged by size: 1471 1135 1132 681 287 219 191 SfoI GGC'GCC Cuts at: 2314 2314 Size: 5116 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5116 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5116 SmaI CCC'GGG Cuts at: 1872 2147 2443 1872 Size: 275 296 4545 Fragments arranged by size: 4545 296 275 SmlI C'TyrA_G Cuts at: 820 1017 1943 2069 3220 4121 4383 820 Size: 197 926 126 1151 901 262 1553 Fragments arranged by size: 1553 1151 926 901 262 197 126 SnaBI TAC'GTA Cuts at: 493 2907 493 Size: 2414 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5116 SphI G_CATG'C Cuts at: 1132 2474 1132 Size: 1342 3774 SspI AAT'ATT Cuts at: 5 52 3040 5 Size: 47 2988 2081 Fragments arranged by size: 2988 2081 47 StuI AGG'CCT Cuts at: 1517 2369 1517 Size: 852 4264 StyI C'CwwG_G Cuts at: 513 1065 2555 513 Size: 552 1490 3074 Fragments arranged by size: 3074 1490 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2184 2272 3143 3160 3313 3498 3943 Size: 1255 604 88 871 17 153 185 445 Cuts at: 3943 325 Size: 1498 Fragments arranged by size: 1498 1255 871 604 445 185 153 88 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3362 5074 96 Size: 274 80 33 51 1443 1385 1712 138 Fragments arranged by size: 1712 1443 1385 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5076 5108 5076 Size: 32 5084 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5116 XbaI T'CTAG_A Cuts at: 2452 2452 Size: 5116 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5116 XmaI C'CCGG_G Cuts at: 1870 2145 2441 1870 Size: 275 296 4545 Fragments arranged by size: 4545 296 275 XmnI GAAnn'nnTTC Cuts at: 3245 3245 Size: 5116 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2924 276 Size: 53 83 186 936 1390 2468 Fragments arranged by size: 2468 1390 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI