(Circular) (Six-base) MAPSORT of: pFN21AB7867.seq Check: 8435 from: 1 to: 6385 pFN21AB7867 6385 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2239 2564 3323 2239 Size: 325 759 5301 Fragments arranged by size: 5301 759 325 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3639 4195 278 Size: 53 83 186 936 2103 556 2468 Fragments arranged by size: 2468 2103 936 556 186 83 53 AccI GT'mk_AC Cuts at: 1937 3728 1937 Size: 1791 4594 Acc65I G'GTAC_C Cuts at: 3706 3706 Size: 6385 AclI AA'CG_TT Cuts at: 1563 4512 4885 1563 Size: 2949 373 3063 Fragments arranged by size: 3063 2949 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4448 5832 6100 1632 Size: 2816 1384 268 1917 Fragments arranged by size: 2816 1917 1384 268 AfeI AGC'GCT Cuts at: 3239 3239 Size: 6385 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6188 AflIII A'CryG_T Cuts at: 1203 2124 5284 6261 1203 Size: 921 3160 977 1327 Fragments arranged by size: 3160 1327 977 921 AgeI A'CCGG_T Cuts at: 5216 5216 Size: 6385 AhdI GACnn_n'nnGTC Cuts at: 5114 5114 Size: 6385 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6385 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6385 AlwNI CAG_nnn'CTG Cuts at: 3082 3338 4128 5700 3082 Size: 256 790 1572 3767 Fragments arranged by size: 3767 1572 790 256 ApaI G_GGCC'C Cuts at: 2690 2690 Size: 6385 ApaLI G'TGCA_C Cuts at: 4442 5598 4442 Size: 1156 5229 ApoI r'AATT_y Cuts at: 1493 3694 3920 4072 6181 1493 Size: 2201 226 152 2109 1697 Fragments arranged by size: 2201 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4939 160 Size: 872 3907 1606 Fragments arranged by size: 3907 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6385 AvaI C'yCGr_G Cuts at: 1870 1943 3383 3488 3710 1870 Size: 73 1440 105 222 4545 Fragments arranged by size: 4545 1440 222 105 73 BamHI G'GATC_C Cuts at: 2064 3715 4082 2064 Size: 1651 367 4367 Fragments arranged by size: 4367 1651 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2251 2542 2971 3532 3706 Size: 325 217 627 464 291 429 561 174 Cuts at: 3706 5161 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 561 464 429 325 291 217 174 BanII G_rGCy'C Cuts at: 1391 2370 2690 3273 3704 1391 Size: 979 320 583 431 4072 Fragments arranged by size: 4072 979 583 431 320 BbeI G_GCGC'C Cuts at: 2546 2975 2546 Size: 429 5956 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2026 928 Size: 971 127 5287 Fragments arranged by size: 5287 971 127 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2776 2810 3092 3126 4576 4610 1903 Size: 34 839 34 282 34 1450 34 3678 Fragments arranged by size: 3678 1450 839 282 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4277 5487 4277 Size: 1210 5175 BclI T'GATC_A Cuts at: 1548 1548 Size: 6385 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2654 3324 4996 Size: 107 122 71 631 605 982 670 1672 Cuts at: 4996 136 Size: 1525 Fragments arranged by size: 1672 1525 982 670 631 605 122 107 71 BglII A'GATC_T Cuts at: 6380 6380 Size: 6385 BlpI GC'TnA_GC Cuts at: 3339 3593 3802 3339 Size: 254 209 5922 Fragments arranged by size: 5922 254 209 Bme1580I G_kGCm'C Cuts at: 1790 2690 4446 5602 1790 Size: 900 1756 1156 2573 Fragments arranged by size: 2573 1756 1156 900 BmgBI CAC'GTC Cuts at: 1318 2032 1318 Size: 714 5671 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4116 5074 442 Size: 416 53 83 399 24 2699 958 1753 Fragments arranged by size: 2699 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6385 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2890 2890 Size: 6385 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2276 2649 3461 3543 5045 1239 Size: 1037 373 812 82 1502 2579 Fragments arranged by size: 2579 1502 1037 812 373 82 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4510 5375 5673 4510 Size: 865 298 5222 Fragments arranged by size: 5222 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3245 5048 882 Size: 617 1746 1803 2219 Fragments arranged by size: 2219 1803 1746 617 BsaAI yAC'GTr Cuts at: 493 1204 2127 3229 3676 4176 493 Size: 711 923 1102 447 500 2702 Fragments arranged by size: 2702 1102 923 711 500 447 BsaBI GATnn'nnATC Cuts at: 6379 6379 Size: 6385 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2543 2972 3500 3636 Size: 53 83 186 936 1010 429 528 136 Cuts at: 3636 4192 4574 5209 275 Size: 556 382 635 1451 Fragments arranged by size: 1451 1010 936 635 556 528 429 382 186 136 83 53 BsaWI w'CCGG_w Cuts at: 2134 4818 5216 5490 5637 6048 2134 Size: 2684 398 274 147 411 2471 Fragments arranged by size: 2684 2471 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2984 3014 1178 Size: 30 165 30 1581 30 4549 Fragments arranged by size: 4549 1581 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 2290 2969 2998 3005 3008 1186 Size: 967 137 679 29 7 3 4563 Fragments arranged by size: 4563 967 679 137 29 7 3 BseYI C'CCAG_C Cuts at: 2546 2879 3331 3467 5588 6084 2546 Size: 333 452 136 2121 496 2847 Fragments arranged by size: 2847 2121 496 452 333 136 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2267 1163 Size: 573 531 5281 Fragments arranged by size: 5281 573 531 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3351 3835 4596 5624 1540 Size: 275 186 1350 484 761 1028 2301 Fragments arranged by size: 2301 1350 1028 761 484 275 186 BsiHKAI G_wGCw'C Cuts at: 2031 2370 2665 4446 4531 5602 2031 Size: 339 295 1781 85 1071 2814 Fragments arranged by size: 2814 1781 1071 339 295 85 BsmI GAATG_Cn' Cuts at: 3896 3989 3896 Size: 93 6292 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2370 2665 2690 3273 3704 4446 Size: 399 241 339 295 25 583 431 742 Cuts at: 4446 4531 5602 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 583 431 399 339 295 241 85 25 BspEI T'CCGG_A Cuts at: 6048 6048 Size: 6385 BspHI T'CATG_A Cuts at: 4274 6004 4274 Size: 1730 4655 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2239 2523 2564 3114 3323 3363 3589 3740 Size: 1395 284 41 550 209 40 226 151 Cuts at: 3740 844 Size: 3489 Fragments arranged by size: 3489 1395 550 284 226 209 151 41 40 BsrBI CCG'CTC Cuts at: 1394 1823 2413 2445 4272 1394 Size: 429 590 32 1827 3507 Fragments arranged by size: 3507 1827 590 429 32 BsrDI GCAATG_nn' Cuts at: 65 1248 4880 5054 65 Size: 1183 3632 174 1396 Fragments arranged by size: 3632 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2285 2648 2707 3032 3496 5029 5216 Size: 110 475 363 59 325 464 1533 187 Cuts at: 5216 1700 Size: 2869 Fragments arranged by size: 2869 1533 475 464 363 325 187 110 59 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6385 BssHII G'CGCG_C Cuts at: 1925 2334 3293 1925 Size: 409 959 5017 Fragments arranged by size: 5017 959 409 BssSI C'ACGA_G Cuts at: 4445 5457 4445 Size: 1012 5373 BstAPI GCAn_nnn'nTGC Cuts at: 794 3082 3240 794 Size: 2288 158 3939 Fragments arranged by size: 3939 2288 158 BstBI TT'CG_AA Cuts at: 5189 5189 Size: 6385 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6385 BstXI CCAn_nnnn'nTGG Cuts at: 3319 3319 Size: 6385 BstYI r'GATC_y Cuts at: 1972 2064 3715 4082 4467 4484 5936 6022 6034 Size: 92 1651 367 385 17 1452 86 12 Cuts at: 6034 6380 1972 Size: 346 1977 Fragments arranged by size: 1977 1651 1452 385 367 346 92 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3256 513 Size: 552 939 1252 3642 Fragments arranged by size: 3642 1252 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5646 Fragments arranged by size: 5646 683 56 BtsI GCAGTG_nn' Cuts at: 3901 4695 4715 3901 Size: 794 20 5571 Fragments arranged by size: 5571 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6385 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6350 DraI TTT'AAA Cuts at: 2935 3689 2935 Size: 754 5631 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3499 5392 809 Size: 507 2183 1893 1802 Fragments arranged by size: 2183 1893 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2530 2646 3348 Size: 54 1425 50 275 18 700 116 702 Cuts at: 3348 3832 4721 6350 8 Size: 484 889 1629 43 Fragments arranged by size: 1629 1425 889 702 700 484 275 116 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3348 3832 1537 Size: 275 1536 484 4090 Fragments arranged by size: 4090 1536 484 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2210 4315 1353 Size: 857 2105 3423 Fragments arranged by size: 3423 2105 857 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3563 4972 5346 5492 387 Size: 1064 138 1974 1409 374 146 1280 Fragments arranged by size: 1974 1409 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2368 2368 Size: 6385 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2276 2649 3461 3543 4448 5045 5832 Size: 393 644 373 812 82 905 597 787 Cuts at: 5832 6100 1239 Size: 268 1524 Fragments arranged by size: 1524 905 812 787 644 597 393 373 268 82 EcoO109I rG'GnC_Cy Cuts at: 2686 2687 3118 2686 Size: 1 431 5953 Fragments arranged by size: 5953 431 1 EcoRI G'AATT_C Cuts at: 3694 4072 3694 Size: 378 6007 EcoRV GAT'ATC Cuts at: 2210 2210 Size: 6385 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3082 3628 3082 Size: 546 5839 FspI TGC'GCA Cuts at: 3233 4891 3233 Size: 1658 4727 HaeII r_GCGC'y Cuts at: 1678 2546 2911 2975 3241 3346 5532 1678 Size: 868 365 64 266 105 2186 2531 Fragments arranged by size: 2531 2186 868 365 266 105 64 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2194 2226 2984 3016 3020 Size: 32 135 32 622 32 758 32 4 Cuts at: 3020 3052 5026 5058 5100 5132 1373 Size: 32 1974 32 42 32 2626 Fragments arranged by size: 2626 1974 758 622 135 42 32 32 32 32 32 32 32 4 HincII GTy'rAC Cuts at: 669 1657 1938 3729 3975 669 Size: 988 281 1791 246 3079 Fragments arranged by size: 3079 1791 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6385 HpaI GTT'AAC Cuts at: 3975 3975 Size: 6385 KasI G'GCGC_C Cuts at: 2542 2971 2542 Size: 429 5956 KpnI G_GTAC'C Cuts at: 3710 3710 Size: 6385 MfeI C'AATT_G Cuts at: 3984 3984 Size: 6385 MluI A'CGCG_T Cuts at: 6261 6261 Size: 6385 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5499 5683 1305 Size: 4194 184 2007 Fragments arranged by size: 4194 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4563 Fragments arranged by size: 4563 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4343 4702 4861 518 Size: 648 3177 359 159 2042 Fragments arranged by size: 3177 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2018 2200 3338 3530 3801 4478 5626 5871 Size: 412 182 1138 192 271 677 1148 245 Cuts at: 5871 1606 Size: 2120 Fragments arranged by size: 2120 1148 1138 677 412 271 245 192 182 NaeI GCC'GGC Cuts at: 2709 3034 2709 Size: 325 6060 NarI GG'CG_CC Cuts at: 2543 2972 2543 Size: 429 5956 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4894 Fragments arranged by size: 4894 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6385 NgoMIV G'CCGG_C Cuts at: 2707 3032 2707 Size: 325 6060 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6385 NotI GC'GGCC_GC Cuts at: 3832 3832 Size: 6385 NspI r_CATG'y Cuts at: 1132 1229 2827 3743 5288 1132 Size: 97 1598 916 1545 2229 Fragments arranged by size: 2229 1598 1545 916 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6385 PciI A'CATG_T Cuts at: 5284 5284 Size: 6385 PflMI CCAn_nnn'nTGG Cuts at: 1108 2021 1108 Size: 913 5472 PfoI T'CCnGG_A Cuts at: 2148 2292 2148 Size: 144 6241 PmeI GTTT'AAAC Cuts at: 3689 3689 Size: 6385 PmlI CAC'GTG Cuts at: 2127 3229 2127 Size: 1102 5283 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4454 4486 5967 5999 4454 Size: 32 1481 32 4840 Fragments arranged by size: 4840 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3118 3118 Size: 6385 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6385 PsiI TTA'TAA Cuts at: 3955 3955 Size: 6385 PspOMI G'GGCC_C Cuts at: 2686 2686 Size: 6385 PstI C_TGCA'G Cuts at: 830 2520 3087 3586 3737 830 Size: 1690 567 499 151 3478 Fragments arranged by size: 3478 1690 567 499 151 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6385 PvuII CAG'CTG Cuts at: 2018 2200 2018 Size: 182 6203 SacI G_AGCT'C Cuts at: 2370 2370 Size: 6385 SalI G'TCGA_C Cuts at: 1936 3727 1936 Size: 1791 4594 SanDI GG'GwC_CC Cuts at: 3118 3118 Size: 6385 SbfI CC_TGCA'GG Cuts at: 3737 3737 Size: 6385 ScaI AGT'ACT Cuts at: 2453 4633 6345 2453 Size: 2180 1712 2493 Fragments arranged by size: 2493 2180 1712 SfcI C'TryA_G Cuts at: 826 1045 2516 3083 3582 3733 4868 5549 5740 Size: 219 1471 567 499 151 1135 681 191 Cuts at: 5740 826 Size: 1471 Fragments arranged by size: 1471 1471 1135 681 567 499 219 191 151 SfoI GGC'GCC Cuts at: 2544 2973 2544 Size: 429 5956 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6385 SgrAI Cr'CCGG_yG Cuts at: 1700 3496 1700 Size: 1796 4589 SmaI CCC'GGG Cuts at: 1872 3712 1872 Size: 1840 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4489 5390 5652 820 Size: 197 926 2546 901 262 1553 Fragments arranged by size: 2546 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4176 493 Size: 3683 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6385 SphI G_CATG'C Cuts at: 1132 2827 3743 1132 Size: 1695 916 3774 Fragments arranged by size: 3774 1695 916 SspI AAT'ATT Cuts at: 5 52 4309 5 Size: 47 4257 2081 Fragments arranged by size: 4257 2081 47 StuI AGG'CCT Cuts at: 1517 3065 1517 Size: 1548 4837 StyI C'CwwG_G Cuts at: 513 1065 2004 3057 3824 513 Size: 552 939 1053 767 3074 Fragments arranged by size: 3074 1053 939 767 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3550 4412 4429 4582 4767 5212 325 Size: 1255 1970 862 17 153 185 445 1498 Fragments arranged by size: 1970 1498 1255 862 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2451 4631 6343 Size: 274 80 33 51 1443 474 2180 1712 Cuts at: 6343 96 Size: 138 Fragments arranged by size: 2180 1712 1443 474 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2775 2807 6345 6377 2775 Size: 32 3538 32 2783 Fragments arranged by size: 3538 2783 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6385 XbaI T'CTAG_A Cuts at: 3721 3721 Size: 6385 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2239 2326 2623 2239 Size: 87 297 6001 Fragments arranged by size: 6001 297 87 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6385 XmaI C'CCGG_G Cuts at: 1870 3710 1870 Size: 1840 4545 XmnI GAAnn'nnTTC Cuts at: 4514 4514 Size: 6385 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3637 4193 276 Size: 53 83 186 936 2103 556 2468 Fragments arranged by size: 2468 2103 936 556 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI