Restriction map for
pFN21AB3164
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# NO CUT ENZYMES ( 102 enzymes) #
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Acc65I AccII AclI AhdI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BbeI BbrPI BciVI BcnI
BglI BlnI BlpI Bpu1102I BsaAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspLU11I BssHII Bst1107I BstEII BstPI
BstUI BstZ17I CelII ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MluNI MscI MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 601 GACGTC GACGT^C
AccI 598 GTAGAC GT^[AC][GT]AC
AccIII 2453 TCCGGA T^CCGGA
AcyI 601 GACGTC G[GA]^CG[CT]C
AflII 826 CTTAAG C^TTAAG
AflIII 534 ACGTGT A^C[GA][CT]GT
AgeI 2279 ACCGGT A^CCGGT
Alw44I 2724 GTGCAC G^TGCAC
ApaLI 2724 GTGCAC G^TGCAC
BanII 2716 GAGCTC G[GA]GC[CT]^C
BbvCI 882 GCTGAGG CCTCAGC(-5/-2)
BcgI 2041 CGAGTAGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1956 TGATCA T^GATCA
BfrI 826 CTTAAG C^TTAAG
BsaBI 1129 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 601 GACGTC G[GA]^CG[CT]C
BseAI 2453 TCCGGA T^CCGGA
Bsp1407I 335 TGTACA T^GTACA
BspEI 2453 TCCGGA T^CCGGA
BspHI 2361 TCATGA T^CATGA
BspMI 2005 GCAGGT ACCTGC(4/8)
BsrBI 832 GAGCGG CCGCTC(-3/-3)
BsrFI 2279 ACCGGT [GA]^CCGG[CT]
BsrGI 335 TGTACA T^GTACA
BstBI 271 TTCGAA TT^CGAA
BstXI 715 CCAAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 2279 ACCGGT [GA]^CCGG[CT]
DraIII 2225 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2 TGGCCG [CT]^GGCC[GA]
EcoT22I 1555 ATGCAT ATGCA^T
FbaI 1956 TGATCA T^GATCA
Hin1I 601 GACGTC G[GA]^CG[CT]C
MamI 1129 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2453 TCCGGA T^CCGGA
NsiI 1555 ATGCAT ATGCA^T
NspI 764 GCATGC [GA]CATG^[CT]
NspV 271 TTCGAA TT^CGAA
PinAI 2279 ACCGGT A^CCGGT
RcaI 2361 TCATGA T^CATGA
SacI 2716 GAGCTC GAGCT^C
SfuI 271 TTCGAA TT^CGAA
SmlI 826 CTTAAG C^T[CT][GA]AG
SpeI 2208 ACTAGT A^CTAGT
SphI 764 GCATGC GCATG^C
SspBI 335 TGTACA T^GTACA
SspI 2132 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 25 enzymes) #
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AocI 1010 CCTCAGG CC^T[ACGT]AGG
2500 CCTCAGG
Asp700I 1063 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2593 GAATCTCTTC
BamHI 777 GGATCC G^GATCC
966 GGATCC
BanI 465 GGCACC G^G[CT][GA]CC
558 GGCACC
BsaI 2446 GAGACC GGTCTC(1/5)
2008 GGTCTC
BsaWI 2279 ACCGGT [AT]^CCGG[AT]
2453 TCCGGA
BseRI 1008 CTCCTC GAGGAG(10/8)
1382 CTCCTC
BsgI 2558 CTGCAC GTGCAG(16/14)
917 GTGCAG
BsrDI 2052 CATTGC GCAATG(2/0)
2553 CATTGC
BstAPI 1656 GCAAAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2527 GCAGAGTTTGC
Bsu36I 1010 CCTCAGG CC^T[ACGT]AGG
2500 CCTCAGG
CfoI 1075 GCGC GCG^C
2222 GCGC
DsaI 820 CCATGG C^C[GA][CT]GG
2057 CCATGG
Eco81I 1010 CCTCAGG CC^T[ACGT]AGG
2500 CCTCAGG
HapII 2280 CCGG C^CGG
2454 CCGG
HhaI 1075 GCGC GCG^C
2222 GCGC
HinP1I 1075 GCGC G^CGC
2222 GCGC
HpaII 2280 CCGG C^CGG
2454 CCGG
MspI 2280 CCGG C^CGG
2454 CCGG
NcoI 820 CCATGG C^CATGG
2057 CCATGG
PflMI 1638 CCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1768 CCAGCTAATGG
ScaI 921 AGTACT AGT^ACT
2396 AGTACT
Van91I 1638 CCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1768 CCAGCTAATGG
XcmI 1226 CCAAAGAGGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1457 CCAGAGTTCGGCTGG
XmnI 1063 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2593 GAATCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BbsI
BbvI BfaI BglII BmyI BpmI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrI
BssKI BssSI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HgaI
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII