(Circular) (Six-base) MAPSORT of: pFN21AB3164.seq Check: 7535 from: 1 to: 7468 pFN21AB3164 7468 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2610 5278 278 Size: 53 83 186 936 1074 2668 2468 Fragments arranged by size: 2668 2468 1074 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2604 4811 1937 Size: 667 2207 4594 Fragments arranged by size: 4594 2207 667 Acc65I G'GTAC_C Cuts at: 4789 4789 Size: 7468 AclI AA'CG_TT Cuts at: 1563 5595 5968 1563 Size: 4032 373 3063 Fragments arranged by size: 4032 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2056 3000 3311 4019 4535 4589 5531 6915 Size: 424 944 311 708 516 54 942 1384 Cuts at: 6915 7183 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 944 942 708 516 424 311 268 54 AflII C'TTAA_G Cuts at: 820 1017 2831 820 Size: 197 1814 5457 Fragments arranged by size: 5457 1814 197 AflIII A'CryG_T Cuts at: 1203 2539 6367 7344 1203 Size: 1336 3828 977 1327 Fragments arranged by size: 3828 1336 1327 977 AgeI A'CCGG_T Cuts at: 4284 6299 4284 Size: 2015 5453 AhdI GACnn_n'nnGTC Cuts at: 6197 6197 Size: 7468 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7468 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2133 2792 1184 Size: 949 659 5860 Fragments arranged by size: 5860 949 659 AlwNI CAG_nnn'CTG Cuts at: 2199 2380 3055 4480 4513 4612 5211 6783 2199 Size: 181 675 1425 33 99 599 1572 2884 Fragments arranged by size: 2884 1572 1425 675 599 181 99 33 ApaLI G'TGCA_C Cuts at: 4729 5525 6681 4729 Size: 796 1156 5516 Fragments arranged by size: 5516 1156 796 ApoI r'AATT_y Cuts at: 1493 2279 2894 2951 3249 3609 4777 5003 5155 Size: 786 615 57 298 360 1168 226 152 Cuts at: 5155 7264 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1168 786 615 360 298 226 152 57 AseI AT'TA_AT Cuts at: 160 1032 6022 160 Size: 872 4990 1606 Fragments arranged by size: 4990 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7468 AvaI C'yCGr_G Cuts at: 1870 1943 4793 1870 Size: 73 2850 4545 Fragments arranged by size: 4545 2850 73 BamHI G'GATC_C Cuts at: 2782 2971 4798 5165 2782 Size: 189 1827 367 5085 Fragments arranged by size: 5085 1827 367 189 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2470 2563 4789 6244 618 Size: 325 217 627 683 93 2226 1455 1842 Fragments arranged by size: 2226 1842 1455 683 627 325 217 93 BanII G_rGCy'C Cuts at: 1391 4725 4787 1391 Size: 3334 62 4072 Fragments arranged by size: 4072 3334 62 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3009 3177 3720 4076 4252 928 Size: 971 1110 168 543 356 176 4144 Fragments arranged by size: 4144 1110 971 543 356 176 168 BbvCI CC'TCA_GC Cuts at: 2888 2888 Size: 7468 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4035 4069 5659 5693 1903 Size: 34 2098 34 1590 34 3678 Fragments arranged by size: 3678 2098 1590 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5360 6570 5360 Size: 1210 6258 BclI T'GATC_A Cuts at: 1548 3961 1548 Size: 2413 5055 BfrBI ATG'CAT Cuts at: 3562 3562 Size: 7468 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6079 136 Size: 107 122 71 631 605 4407 1525 Fragments arranged by size: 4407 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2176 2296 3157 3846 4159 7463 2176 Size: 120 861 689 313 3304 2181 Fragments arranged by size: 3304 2181 861 689 313 120 BlpI GC'TnA_GC Cuts at: 4885 4885 Size: 7468 Bme1580I G_kGCm'C Cuts at: 1790 4733 5529 6685 1790 Size: 2943 796 1156 2573 Fragments arranged by size: 2943 2573 1156 796 BmgBI CAC'GTC Cuts at: 1318 2020 2540 4498 1318 Size: 702 520 1958 4288 Fragments arranged by size: 4288 1958 702 520 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2115 5199 6157 Size: 416 53 83 399 24 698 3084 958 Cuts at: 6157 442 Size: 1753 Fragments arranged by size: 3084 1753 958 698 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7468 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2553 3405 4469 4563 4727 6128 1239 Size: 1314 852 1064 94 164 1401 2579 Fragments arranged by size: 2579 1401 1314 1064 852 164 94 Bpu10I CC'TnA_GC Cuts at: 2888 3671 2888 Size: 783 6685 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5593 6458 6756 5593 Size: 865 298 6305 Fragments arranged by size: 6305 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4019 4445 6131 882 Size: 617 2520 426 1686 2219 Fragments arranged by size: 2520 2219 1686 617 426 BsaAI yAC'GTr Cuts at: 493 1204 5259 493 Size: 711 4055 2702 Fragments arranged by size: 4055 2702 711 BsaBI GATnn'nnATC Cuts at: 3138 7462 3138 Size: 4324 3144 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2607 5275 5657 6292 Size: 53 83 186 936 1074 2668 382 635 Cuts at: 6292 275 Size: 1451 Fragments arranged by size: 2668 1451 1074 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4284 4458 5901 6299 6573 6720 7131 4284 Size: 174 1443 398 274 147 411 4621 Fragments arranged by size: 4621 1443 411 398 274 174 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2526 2556 2996 3026 3373 Size: 30 165 30 1123 30 440 30 347 Cuts at: 3373 3403 4303 4333 4561 4591 1178 Size: 30 900 30 228 30 4055 Fragments arranged by size: 4055 1123 900 440 347 228 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3004 3378 1186 Size: 1818 374 5276 Fragments arranged by size: 5276 1818 374 BseYI C'CCAG_C Cuts at: 3772 6671 7167 3772 Size: 2899 496 4073 Fragments arranged by size: 4073 2899 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2943 4548 1163 Size: 573 1207 1605 4083 Fragments arranged by size: 4083 1605 1207 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4918 5679 6707 1540 Size: 275 186 2917 761 1028 2301 Fragments arranged by size: 2917 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3862 4725 4733 5529 5614 6685 3862 Size: 863 8 796 85 1071 4645 Fragments arranged by size: 4645 1071 863 796 85 8 BsmI GAATG_Cn' Cuts at: 2265 3915 4196 4979 5072 2265 Size: 1650 281 783 93 4661 Fragments arranged by size: 4661 1650 783 281 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3862 4725 4733 4787 5529 5614 6685 Size: 399 2072 863 8 54 742 85 1071 Cuts at: 6685 1391 Size: 2174 Fragments arranged by size: 2174 2072 1071 863 742 399 85 54 8 BspEI T'CCGG_A Cuts at: 4458 7131 4458 Size: 2673 4795 BspHI T'CATG_A Cuts at: 4366 5357 7087 4366 Size: 991 1730 4747 Fragments arranged by size: 4747 1730 991 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4001 4823 844 Size: 3157 822 3489 Fragments arranged by size: 3489 3157 822 BsrBI CCG'CTC Cuts at: 1394 1823 2839 5355 1394 Size: 429 1016 2516 3507 Fragments arranged by size: 3507 2516 1016 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4056 4557 5963 6137 65 Size: 1183 2808 501 1406 174 1396 Fragments arranged by size: 2808 1406 1396 1183 501 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4284 6112 6299 1700 Size: 110 2474 1828 187 2869 Fragments arranged by size: 2869 2474 1828 187 110 BsrGI T'GTAC_A Cuts at: 96 2340 96 Size: 2244 5224 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7468 BssSI C'ACGA_G Cuts at: 4421 4469 4724 5528 6540 4421 Size: 48 255 804 1012 5349 Fragments arranged by size: 5349 1012 804 255 48 BstAPI GCAn_nnn'nTGC Cuts at: 794 3667 4538 794 Size: 2873 871 3724 Fragments arranged by size: 3724 2873 871 BstBI TT'CG_AA Cuts at: 2277 6272 2277 Size: 3995 3473 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7468 BstXI CCAn_nnnn'nTGG Cuts at: 2727 2727 Size: 7468 BstYI r'GATC_y Cuts at: 1972 2176 2296 2782 2971 3157 3846 4159 4464 Size: 204 120 486 189 186 689 313 305 Cuts at: 4464 4798 5165 5550 5567 7019 7105 7117 7463 Size: 334 367 385 17 1452 86 12 346 Cuts at: 7463 1972 Size: 1977 Fragments arranged by size: 1977 1452 689 486 385 367 346 334 313 305 204 189 186 120 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3016 4506 3016 Size: 1490 5978 BtgI C'CryG_G Cuts at: 513 1065 2004 2825 4062 513 Size: 552 939 821 1237 3919 Fragments arranged by size: 3919 1237 939 821 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3228 4445 487 Size: 683 56 2002 1217 3510 Fragments arranged by size: 3510 2002 1217 683 56 BtsI GCAGTG_nn' Cuts at: 2732 3100 4984 5778 5798 2732 Size: 368 1884 794 20 4402 Fragments arranged by size: 4402 1884 794 368 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7468 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7433 DraI TTT'AAA Cuts at: 3408 3519 4587 4772 3408 Size: 111 1068 185 6104 Fragments arranged by size: 6104 1068 185 111 DraIII CAC_nnn'GTG Cuts at: 4235 4235 Size: 7468 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6475 809 Size: 507 5159 1802 Fragments arranged by size: 5159 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 4915 5804 Size: 54 1425 50 275 18 177 2908 889 Cuts at: 5804 7433 8 Size: 1629 43 Fragments arranged by size: 2908 1629 1425 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4915 1537 Size: 275 3103 4090 Fragments arranged by size: 4090 3103 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2976 3063 4608 5398 1353 Size: 1623 87 1545 790 3423 Fragments arranged by size: 3423 1623 1545 790 87 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6055 6429 6575 387 Size: 1064 138 4466 374 146 1280 Fragments arranged by size: 4466 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4723 4723 Size: 7468 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2056 2553 3000 3311 3405 4019 4469 Size: 393 424 497 447 311 94 614 450 Cuts at: 4469 4535 4563 4589 4727 5531 6128 6915 7183 Size: 66 28 26 138 804 597 787 268 Cuts at: 7183 1239 Size: 1524 Fragments arranged by size: 1524 804 787 614 597 497 450 447 424 393 311 268 138 94 66 28 26 EcoO109I rG'GnC_Cy Cuts at: 2066 3103 4519 4747 2066 Size: 1037 1416 228 4787 Fragments arranged by size: 4787 1416 1037 228 EcoRI G'AATT_C Cuts at: 4777 5155 4777 Size: 378 7090 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2086 2604 3608 2086 Size: 518 1004 5946 Fragments arranged by size: 5946 1004 518 FspI TGC'GCA Cuts at: 5974 5974 Size: 7468 HaeII r_GCGC'y Cuts at: 1678 6615 1678 Size: 4937 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2076 2108 2767 2799 2996 Size: 32 135 32 504 32 659 32 197 Cuts at: 2996 3028 3803 3831 3835 3863 4583 4615 6109 Size: 32 775 28 4 28 720 32 1494 Cuts at: 6109 6141 6183 6215 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1494 775 720 659 504 197 135 42 32 32 32 32 32 32 32 32 28 28 4 HincII GTy'rAC Cuts at: 669 1657 1938 4812 5058 669 Size: 988 281 2874 246 3079 Fragments arranged by size: 3079 2874 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7468 HpaI GTT'AAC Cuts at: 5058 5058 Size: 7468 KpnI G_GTAC'C Cuts at: 4793 4793 Size: 7468 MfeI C'AATT_G Cuts at: 5067 5067 Size: 7468 MluI A'CGCG_T Cuts at: 7344 7344 Size: 7468 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2524 6582 6766 1305 Size: 1219 4058 184 2007 Fragments arranged by size: 4058 2007 1219 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5646 Fragments arranged by size: 5646 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2424 2774 3446 3600 3632 4061 4425 Size: 648 1258 350 672 154 32 429 364 Cuts at: 4425 5426 5785 5944 518 Size: 1001 359 159 2042 Fragments arranged by size: 2042 1258 1001 672 648 429 364 359 350 159 154 32 MspA1I CmG'CkG Cuts at: 1606 2789 2887 2936 4095 4884 5561 6709 6954 Size: 1183 98 49 1159 789 677 1148 245 Cuts at: 6954 1606 Size: 2120 Fragments arranged by size: 2120 1183 1159 1148 789 677 245 98 49 NcoI C'CATG_G Cuts at: 513 1065 2004 2825 4062 513 Size: 552 939 821 1237 3919 Fragments arranged by size: 3919 1237 939 821 552 NdeI CA'TA_TG Cuts at: 387 2205 4171 4645 387 Size: 1818 1966 474 3210 Fragments arranged by size: 3210 1966 1818 474 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7468 NotI GC'GGCC_GC Cuts at: 4915 4915 Size: 7468 NsiI A_TGCA'T Cuts at: 3564 3564 Size: 7468 NspI r_CATG'y Cuts at: 1132 1229 2773 4826 6371 1132 Size: 97 1544 2053 1545 2229 Fragments arranged by size: 2229 2053 1545 1544 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7468 PciI A'CATG_T Cuts at: 6367 6367 Size: 7468 PflMI CCAn_nnn'nTGG Cuts at: 1108 3649 3779 1108 Size: 2541 130 4797 Fragments arranged by size: 4797 2541 130 PfoI T'CCnGG_A Cuts at: 3129 3129 Size: 7468 PmeI GTTT'AAAC Cuts at: 4772 4772 Size: 7468 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5537 5569 7050 7082 5537 Size: 32 1481 32 5923 Fragments arranged by size: 5923 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2066 3103 4519 4747 2066 Size: 1037 1416 228 4787 Fragments arranged by size: 4787 1416 1037 228 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7468 PsiI TTA'TAA Cuts at: 5038 5038 Size: 7468 PstI C_TGCA'G Cuts at: 830 3095 4012 4534 4617 4820 830 Size: 2265 917 522 83 203 3478 Fragments arranged by size: 3478 2265 917 522 203 83 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7468 PvuII CAG'CTG Cuts at: 2789 2887 4095 2789 Size: 98 1208 6162 Fragments arranged by size: 6162 1208 98 SacI G_AGCT'C Cuts at: 4725 4725 Size: 7468 SalI G'TCGA_C Cuts at: 1936 4810 1936 Size: 2874 4594 SanDI GG'GwC_CC Cuts at: 2066 4747 2066 Size: 2681 4787 SbfI CC_TGCA'GG Cuts at: 4820 4820 Size: 7468 ScaI AGT'ACT Cuts at: 2928 4403 5716 7428 2928 Size: 1475 1313 1712 2968 Fragments arranged by size: 2968 1712 1475 1313 SfcI C'TryA_G Cuts at: 826 1045 2352 2601 3091 4008 4530 4613 4816 Size: 219 1307 249 490 917 522 83 203 Cuts at: 4816 5951 6632 6823 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1307 1135 917 681 522 490 249 219 203 191 83 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7468 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7468 SmaI CCC'GGG Cuts at: 1872 4795 1872 Size: 2923 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2831 5572 6473 6735 820 Size: 197 926 888 2741 901 262 1553 Fragments arranged by size: 2741 1553 926 901 888 262 197 SnaBI TAC'GTA Cuts at: 493 5259 493 Size: 4766 2702 SpeI A'CTAG_T Cuts at: 152 4213 152 Size: 4061 3407 SphI G_CATG'C Cuts at: 1132 2773 4826 1132 Size: 1641 2053 3774 Fragments arranged by size: 3774 2053 1641 SspI AAT'ATT Cuts at: 5 52 4139 5392 5 Size: 47 4087 1253 2081 Fragments arranged by size: 4087 2081 1253 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7468 StyI C'CwwG_G Cuts at: 513 1065 2004 2670 2720 2825 3882 4062 4907 Size: 552 939 666 50 105 1057 180 845 Cuts at: 4907 513 Size: 3074 Fragments arranged by size: 3074 1057 939 845 666 552 180 105 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2838 4293 5495 5512 5665 5850 6295 Size: 1255 1258 1455 1202 17 153 185 445 Cuts at: 6295 325 Size: 1498 Fragments arranged by size: 1498 1455 1258 1255 1202 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2168 2340 2926 Size: 274 80 33 51 1443 191 172 586 Cuts at: 2926 3220 4097 4125 4401 5714 7426 96 Size: 294 877 28 276 1313 1712 138 Fragments arranged by size: 1712 1443 1313 877 586 294 276 274 191 172 138 80 51 33 28 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2140 2172 3373 3405 4503 4535 4731 4763 7428 Size: 32 1201 32 1098 32 196 32 2665 Cuts at: 7428 7460 2140 Size: 32 2148 Fragments arranged by size: 2665 2148 1201 1098 196 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7468 XbaI T'CTAG_A Cuts at: 4804 4804 Size: 7468 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3238 3469 3238 Size: 231 7237 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7468 XmaI C'CCGG_G Cuts at: 1870 4793 1870 Size: 2923 4545 XmnI GAAnn'nnTTC Cuts at: 3072 4602 5597 3072 Size: 1530 995 4943 Fragments arranged by size: 4943 1530 995 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2608 5276 276 Size: 53 83 186 936 1074 2668 2468 Fragments arranged by size: 2668 2468 1074 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI