(Circular) (Six-base) MAPSORT of: pFN21AE0354.seq Check: 8623 from: 1 to: 6862 pFN21AE0354 6862 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4076 4076 Size: 6862 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2460 4672 278 Size: 53 83 186 936 924 2212 2468 Fragments arranged by size: 2468 2212 936 924 186 83 53 AccI GT'mk_AC Cuts at: 1937 4205 1937 Size: 2268 4594 Acc65I G'GTAC_C Cuts at: 4183 4183 Size: 6862 AclI AA'CG_TT Cuts at: 1563 4989 5362 1563 Size: 3426 373 3063 Fragments arranged by size: 3426 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4925 6309 6577 1632 Size: 3293 1384 268 1917 Fragments arranged by size: 3293 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6665 AflIII A'CryG_T Cuts at: 1203 2292 2781 3357 5761 6738 1203 Size: 1089 489 576 2404 977 1327 Fragments arranged by size: 2404 1327 1089 977 576 489 AgeI A'CCGG_T Cuts at: 5693 5693 Size: 6862 AhdI GACnn_n'nnGTC Cuts at: 3619 5591 3619 Size: 1972 4890 AleI CACnn'nnGTG Cuts at: 1166 2291 2828 3584 1166 Size: 1125 537 756 4444 Fragments arranged by size: 4444 1125 756 537 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6862 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2948 2980 2948 Size: 32 6830 AlwNI CAG_nnn'CTG Cuts at: 3679 3792 3805 4096 4605 6177 3679 Size: 113 13 291 509 1572 4364 Fragments arranged by size: 4364 1572 509 291 113 13 ApaI G_GGCC'C Cuts at: 2539 3044 3213 3248 3635 3757 2539 Size: 505 169 35 387 122 5644 Fragments arranged by size: 5644 505 387 169 122 35 ApaLI G'TGCA_C Cuts at: 3274 4919 6075 3274 Size: 1645 1156 4061 Fragments arranged by size: 4061 1645 1156 ApoI r'AATT_y Cuts at: 1493 4171 4397 4549 6658 1493 Size: 2678 226 152 2109 1697 Fragments arranged by size: 2678 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5416 160 Size: 872 4384 1606 Fragments arranged by size: 4384 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6862 AvaI C'yCGr_G Cuts at: 1870 1943 4187 1870 Size: 73 2244 4545 Fragments arranged by size: 4545 2244 73 BamHI G'GATC_C Cuts at: 2133 4192 4559 2133 Size: 2059 367 4436 Fragments arranged by size: 4436 2059 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2070 2720 3312 3511 4183 Size: 325 217 627 283 650 592 199 672 Cuts at: 4183 5638 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 672 650 627 592 325 283 217 199 BanII G_rGCy'C Cuts at: 1391 2539 3029 3044 3213 3248 3635 3757 3786 Size: 1148 490 15 169 35 387 122 29 Cuts at: 3786 4181 1391 Size: 395 4072 Fragments arranged by size: 4072 1148 490 395 387 169 122 35 29 15 BbeI G_GCGC'C Cuts at: 2074 2724 3316 2074 Size: 650 592 5620 Fragments arranged by size: 5620 650 592 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2280 2410 3553 928 Size: 971 381 130 1143 4237 Fragments arranged by size: 4237 1143 971 381 130 BbvCI CC'TCA_GC Cuts at: 3452 3938 3452 Size: 486 6376 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2066 2100 5053 5087 1903 Size: 34 129 34 2953 34 3678 Fragments arranged by size: 3678 2953 129 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2364 4754 5964 2364 Size: 2390 1210 3262 Fragments arranged by size: 3262 2390 1210 BclI T'GATC_A Cuts at: 1548 3603 1548 Size: 2055 4807 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5473 136 Size: 107 122 71 631 605 3801 1525 Fragments arranged by size: 3801 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3012 6857 3012 Size: 3845 3017 BlpI GC'TnA_GC Cuts at: 2253 3023 4279 2253 Size: 770 1256 4836 Fragments arranged by size: 4836 1256 770 Bme1580I G_kGCm'C Cuts at: 1790 2539 2801 3044 3213 3241 3248 3278 3635 Size: 749 262 243 169 28 7 30 357 Cuts at: 3635 3757 4923 6079 1790 Size: 122 1166 1156 2573 Fragments arranged by size: 2573 1166 1156 749 357 262 243 169 122 30 28 7 BmgBI CAC'GTC Cuts at: 1318 2293 3070 3469 3601 1318 Size: 975 777 399 132 4579 Fragments arranged by size: 4579 975 777 399 132 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4593 5551 442 Size: 416 53 83 399 24 3176 958 1753 Fragments arranged by size: 3176 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6862 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3928 3928 Size: 6862 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2567 2905 3194 3611 3668 3701 3705 3761 Size: 1328 338 289 417 57 33 4 56 Cuts at: 3761 3893 5522 1239 Size: 132 1629 2579 Fragments arranged by size: 2579 1629 1328 417 338 289 132 57 56 33 4 Bpu10I CC'TnA_GC Cuts at: 3452 3785 3938 3452 Size: 333 153 6376 Fragments arranged by size: 6376 333 153 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4987 5852 6150 4987 Size: 865 298 5699 Fragments arranged by size: 5699 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5525 882 Size: 617 4026 2219 Fragments arranged by size: 4026 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3358 4653 493 Size: 711 2154 1295 2702 Fragments arranged by size: 2702 2154 1295 711 BsaBI GATnn'nnATC Cuts at: 6856 6856 Size: 6862 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2071 2415 2457 2721 Size: 53 83 186 936 538 344 42 264 Cuts at: 2721 3313 4669 5051 5686 275 Size: 592 1356 382 635 1451 Fragments arranged by size: 1451 1356 936 635 592 538 382 344 264 186 83 53 42 BsaWI w'CCGG_w Cuts at: 5295 5693 5967 6114 6525 5295 Size: 398 274 147 411 5632 Fragments arranged by size: 5632 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2296 2326 3641 3653 3671 Size: 30 165 30 893 30 1315 12 18 Cuts at: 3671 3683 1178 Size: 12 4357 Fragments arranged by size: 4357 1315 893 165 30 30 30 18 12 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 2981 3200 3389 3704 1186 Size: 877 918 219 189 315 4344 Fragments arranged by size: 4344 918 877 315 219 189 BseYI C'CCAG_C Cuts at: 2054 2191 2904 3037 3044 3090 3222 3247 3382 Size: 137 713 133 7 46 132 25 135 Cuts at: 3382 3652 6065 6561 2054 Size: 270 2413 496 2355 Fragments arranged by size: 2413 2355 713 496 270 137 135 133 132 46 25 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3029 1163 Size: 573 1293 4996 Fragments arranged by size: 4996 1293 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3442 3585 4312 5073 6101 1540 Size: 275 186 1441 143 727 761 1028 2301 Fragments arranged by size: 2301 1441 1028 761 727 275 186 143 BsiHKAI G_wGCw'C Cuts at: 2649 3278 3521 4923 5008 6079 2649 Size: 629 243 1402 85 1071 3432 Fragments arranged by size: 3432 1402 1071 629 243 85 BsmI GAATG_Cn' Cuts at: 3958 4373 4466 3958 Size: 415 93 6354 Fragments arranged by size: 6354 415 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2539 2649 2801 3029 3044 3213 3241 Size: 399 749 110 152 228 15 169 28 Cuts at: 3241 3248 3278 3521 3635 3757 3786 4181 4923 Size: 7 30 243 114 122 29 395 742 Cuts at: 4923 5008 6079 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 749 742 399 395 243 228 169 152 122 114 110 85 30 29 28 15 7 BspEI T'CCGG_A Cuts at: 6525 6525 Size: 6862 BspHI T'CATG_A Cuts at: 4751 6481 4751 Size: 1730 5132 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3624 3648 4076 4217 844 Size: 2780 24 428 141 3489 Fragments arranged by size: 3489 2780 428 141 24 BsrBI CCG'CTC Cuts at: 1394 1823 3444 4039 4749 1394 Size: 429 1621 595 710 3507 Fragments arranged by size: 3507 1621 710 595 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3732 5357 5531 65 Size: 1183 2484 1625 174 1396 Fragments arranged by size: 2484 1625 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3306 3530 5506 5693 1700 Size: 110 1496 224 1976 187 2869 Fragments arranged by size: 2869 1976 1496 224 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6862 BssHII G'CGCG_C Cuts at: 1925 3433 1925 Size: 1508 5354 BssSI C'ACGA_G Cuts at: 2039 2714 4922 5934 2039 Size: 675 2208 1012 2967 Fragments arranged by size: 2967 2208 1012 675 BstAPI GCAn_nnn'nTGC Cuts at: 794 3805 3953 4077 794 Size: 3011 148 124 3579 Fragments arranged by size: 3579 3011 148 124 BstBI TT'CG_AA Cuts at: 5666 5666 Size: 6862 BstEII G'GTnAC_C Cuts at: 1184 2026 1184 Size: 842 6020 BstYI r'GATC_y Cuts at: 1972 2133 3012 4192 4559 4944 4961 6413 6499 Size: 161 879 1180 367 385 17 1452 86 Cuts at: 6499 6511 6857 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1180 879 385 367 346 161 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2569 2855 3585 513 Size: 552 939 565 286 730 3790 Fragments arranged by size: 3790 939 730 565 552 286 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2946 487 Size: 683 56 1720 4403 Fragments arranged by size: 4403 1720 683 56 BtsI GCAGTG_nn' Cuts at: 2023 2724 3815 4081 4378 5172 5192 2023 Size: 701 1091 266 297 794 20 3693 Fragments arranged by size: 3693 1091 794 701 297 266 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6862 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6827 DraI TTT'AAA Cuts at: 4166 4166 Size: 6862 DraIII CAC_nnn'GTG Cuts at: 2809 2925 2809 Size: 116 6746 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5869 809 Size: 507 4553 1802 Fragments arranged by size: 4553 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2443 2596 2700 Size: 54 1425 50 275 18 613 153 104 Cuts at: 2700 3582 4149 4309 5198 6827 8 Size: 882 567 160 889 1629 43 Fragments arranged by size: 1629 1425 889 882 613 567 275 160 153 104 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3582 4309 1537 Size: 275 1770 727 4090 Fragments arranged by size: 4090 1770 727 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2754 4792 1353 Size: 1401 2038 3423 Fragments arranged by size: 3423 2038 1401 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5449 5823 5969 387 Size: 1064 138 3860 374 146 1280 Fragments arranged by size: 3860 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2567 2905 3194 3611 3668 3701 3705 Size: 393 935 338 289 417 57 33 4 Cuts at: 3705 3761 3893 4925 5522 6309 6577 1239 Size: 56 132 1032 597 787 268 1524 Fragments arranged by size: 1524 1032 935 787 597 417 393 338 289 268 132 57 56 33 4 EcoNI CCTnn'n_nnAGG Cuts at: 2549 2702 2771 2549 Size: 153 69 6640 Fragments arranged by size: 6640 153 69 EcoO109I rG'GnC_Cy Cuts at: 2235 2536 2764 2880 3041 3209 3210 3244 3328 Size: 301 228 116 161 168 1 34 84 Cuts at: 3328 3631 3632 3753 3789 2235 Size: 303 1 121 36 5308 Fragments arranged by size: 5308 303 301 228 168 161 121 116 84 36 34 1 1 EcoRI G'AATT_C Cuts at: 4171 4549 4171 Size: 378 6484 FspI TGC'GCA Cuts at: 5368 5368 Size: 6862 HaeII r_GCGC'y Cuts at: 1678 2074 2724 3316 3459 3574 6009 1678 Size: 396 650 592 143 115 2435 2531 Fragments arranged by size: 2531 2435 650 592 396 143 115 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3136 3168 3605 3637 5503 Size: 32 135 32 1564 32 437 32 1866 Cuts at: 5503 5535 5577 5609 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1866 1564 437 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4206 4452 669 Size: 988 281 2268 246 3079 Fragments arranged by size: 3079 2268 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6862 HpaI GTT'AAC Cuts at: 4452 4452 Size: 6862 KasI G'GCGC_C Cuts at: 2070 2720 3312 2070 Size: 650 592 5620 Fragments arranged by size: 5620 650 592 KpnI G_GTAC'C Cuts at: 4187 4187 Size: 6862 MfeI C'AATT_G Cuts at: 4461 4461 Size: 6862 MluI A'CGCG_T Cuts at: 2781 6738 2781 Size: 3957 2905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2041 3639 5976 6160 1305 Size: 736 1598 2337 184 2007 Fragments arranged by size: 2337 2007 1598 736 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2702 4151 10 Size: 54 1425 343 870 1449 2721 Fragments arranged by size: 2721 1449 1425 870 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2291 2604 2828 3584 4098 4820 5179 Size: 648 1125 313 224 756 514 722 359 Cuts at: 5179 5338 518 Size: 159 2042 Fragments arranged by size: 2042 1125 756 722 648 514 359 313 224 159 MspA1I CmG'CkG Cuts at: 1606 2191 2448 2521 2895 3090 3480 3667 3802 Size: 585 257 73 374 195 390 187 135 Cuts at: 3802 3982 4278 4955 6103 6348 1606 Size: 180 296 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 585 390 374 296 257 245 195 187 180 135 73 NaeI GCC'GGC Cuts at: 3532 3532 Size: 6862 NarI GG'CG_CC Cuts at: 2071 2721 3313 2071 Size: 650 592 5620 Fragments arranged by size: 5620 650 592 NcoI C'CATG_G Cuts at: 513 1065 2004 2855 513 Size: 552 939 851 4520 Fragments arranged by size: 4520 939 851 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6862 NgoMIV G'CCGG_C Cuts at: 3530 3530 Size: 6862 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6862 NotI GC'GGCC_GC Cuts at: 4309 4309 Size: 6862 NspI r_CATG'y Cuts at: 1132 1229 4220 5765 1132 Size: 97 2991 1545 2229 Fragments arranged by size: 2991 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3213 1348 Size: 1865 4997 PciI A'CATG_T Cuts at: 5761 5761 Size: 6862 PflMI CCAn_nnn'nTGG Cuts at: 1108 2041 2698 3505 4096 1108 Size: 933 657 807 591 3874 Fragments arranged by size: 3874 933 807 657 591 PfoI T'CCnGG_A Cuts at: 2383 2544 3870 2383 Size: 161 1326 5375 Fragments arranged by size: 5375 1326 161 PmeI GTTT'AAAC Cuts at: 4166 4166 Size: 6862 PmlI CAC'GTG Cuts at: 3358 3358 Size: 6862 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2246 2278 4931 4963 6444 6476 2246 Size: 32 2653 32 1481 32 2632 Fragments arranged by size: 2653 2632 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3789 3789 Size: 6862 PshAI GACnn'nnGTC Cuts at: 1613 2295 1613 Size: 682 6180 PsiI TTA'TAA Cuts at: 4432 4432 Size: 6862 PspOMI G'GGCC_C Cuts at: 2535 3040 3209 3244 3631 3753 2535 Size: 505 169 35 387 122 5644 Fragments arranged by size: 5644 505 387 169 122 35 PstI C_TGCA'G Cuts at: 830 2079 3235 3621 3810 4088 4214 830 Size: 1249 1156 386 189 278 126 3478 Fragments arranged by size: 3478 1249 1156 386 278 189 126 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6862 PvuII CAG'CTG Cuts at: 2191 2895 3667 3802 3982 2191 Size: 704 772 135 180 5071 Fragments arranged by size: 5071 772 704 180 135 SalI G'TCGA_C Cuts at: 1936 4204 1936 Size: 2268 4594 SbfI CC_TGCA'GG Cuts at: 4214 4214 Size: 6862 ScaI AGT'ACT Cuts at: 5110 6822 5110 Size: 1712 5150 SexAI A'CCwGG_T Cuts at: 3504 3504 Size: 6862 SfcI C'TryA_G Cuts at: 826 1045 2075 3231 3617 3806 4084 4210 5345 Size: 219 1030 1156 386 189 278 126 1135 Cuts at: 5345 6026 6217 826 Size: 681 191 1471 Fragments arranged by size: 1471 1156 1135 1030 681 386 278 219 191 189 126 SfoI GGC'GCC Cuts at: 2072 2722 3314 2072 Size: 650 592 5620 Fragments arranged by size: 5620 650 592 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6862 SgrAI Cr'CCGG_yG Cuts at: 1700 3530 1700 Size: 1830 5032 SmaI CCC'GGG Cuts at: 1872 4189 1872 Size: 2317 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4966 5867 6129 820 Size: 197 926 3023 901 262 1553 Fragments arranged by size: 3023 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4653 493 Size: 4160 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6862 SphI G_CATG'C Cuts at: 1132 4220 1132 Size: 3088 3774 SspI AAT'ATT Cuts at: 5 52 4786 5 Size: 47 4734 2081 Fragments arranged by size: 4734 2081 47 StuI AGG'CCT Cuts at: 1517 2555 3722 1517 Size: 1038 1167 4657 Fragments arranged by size: 4657 1167 1038 StyI C'CwwG_G Cuts at: 513 1065 2004 2176 2703 2826 2855 3082 3324 Size: 552 939 172 527 123 29 227 242 Cuts at: 3324 4301 513 Size: 977 3074 Fragments arranged by size: 3074 977 939 552 527 242 227 172 123 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3082 4889 4906 5059 5244 5689 325 Size: 1255 1502 1807 17 153 185 445 1498 Fragments arranged by size: 1807 1502 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5108 6820 96 Size: 274 80 33 51 1443 3131 1712 138 Fragments arranged by size: 3131 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2771 2803 6822 6854 2771 Size: 32 4019 32 2779 Fragments arranged by size: 4019 2779 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6862 XbaI T'CTAG_A Cuts at: 4198 4198 Size: 6862 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2038 4097 2038 Size: 2059 4803 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6862 XmaI C'CCGG_G Cuts at: 1870 4187 1870 Size: 2317 4545 XmnI GAAnn'nnTTC Cuts at: 2528 4991 2528 Size: 2463 4399 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2458 4670 276 Size: 53 83 186 936 924 2212 2468 Fragments arranged by size: 2468 2212 936 924 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI