(Circular) (Six-base) MAPSORT of: pFN21AB8435.seq Check: 25 from: 1 to: 6214 pFN21AB8435 6214 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4024 278 Size: 53 83 186 936 2488 2468 Fragments arranged by size: 2488 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3557 1937 Size: 1620 4594 Acc65I G'GTAC_C Cuts at: 3535 3535 Size: 6214 AclI AA'CG_TT Cuts at: 1563 4341 4714 1563 Size: 2778 373 3063 Fragments arranged by size: 3063 2778 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2228 2754 2825 3246 4277 5661 5929 1632 Size: 596 526 71 421 1031 1384 268 1917 Fragments arranged by size: 1917 1384 1031 596 526 421 268 71 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6017 AflIII A'CryG_T Cuts at: 1203 3331 5113 6090 1203 Size: 2128 1782 977 1327 Fragments arranged by size: 2128 1782 1327 977 AgeI A'CCGG_T Cuts at: 5045 5045 Size: 6214 AhdI GACnn_n'nnGTC Cuts at: 2096 2704 4943 2096 Size: 608 2239 3367 Fragments arranged by size: 3367 2239 608 AleI CACnn'nnGTG Cuts at: 1166 2015 1166 Size: 849 5365 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6214 AlwNI CAG_nnn'CTG Cuts at: 2176 2349 2365 2761 3957 5529 2176 Size: 173 16 396 1196 1572 2861 Fragments arranged by size: 2861 1572 1196 396 173 16 ApaLI G'TGCA_C Cuts at: 3335 3473 4271 5427 3335 Size: 138 798 1156 4122 Fragments arranged by size: 4122 1156 798 138 ApoI r'AATT_y Cuts at: 1493 2941 3523 3749 3901 6010 1493 Size: 1448 582 226 152 2109 1697 Fragments arranged by size: 2109 1697 1448 582 226 152 AscI GG'CGCG_CC Cuts at: 3492 3492 Size: 6214 AseI AT'TA_AT Cuts at: 160 1032 4768 160 Size: 872 3736 1606 Fragments arranged by size: 3736 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 AvaI C'yCGr_G Cuts at: 1870 1943 3487 3539 1870 Size: 73 1544 52 4545 Fragments arranged by size: 4545 1544 73 52 BamHI G'GATC_C Cuts at: 3544 3911 3544 Size: 367 5847 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2690 3444 3535 4990 618 Size: 325 217 627 903 754 91 1455 1842 Fragments arranged by size: 1842 1455 903 754 627 325 217 91 BanII G_rGCy'C Cuts at: 1391 2298 3533 1391 Size: 907 1235 4072 Fragments arranged by size: 4072 1235 907 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2222 2263 2310 3060 3108 928 Size: 971 323 41 47 750 48 4034 Fragments arranged by size: 4034 971 750 323 48 47 41 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2321 2355 4405 4439 1903 Size: 34 384 34 2050 34 3678 Fragments arranged by size: 3678 2050 384 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2363 4106 5316 2363 Size: 1743 1210 3261 Fragments arranged by size: 3261 1743 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6214 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2798 3456 4825 Size: 107 122 71 631 605 1126 658 1369 Cuts at: 4825 136 Size: 1525 Fragments arranged by size: 1525 1369 1126 658 631 605 122 107 71 BglII A'GATC_T Cuts at: 6209 6209 Size: 6214 BlpI GC'TnA_GC Cuts at: 3631 3631 Size: 6214 Bme1580I G_kGCm'C Cuts at: 1790 3339 3477 4275 5431 1790 Size: 1549 138 798 1156 2573 Fragments arranged by size: 2573 1549 1156 798 138 BmgBI CAC'GTC Cuts at: 1318 3340 1318 Size: 2022 4192 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3242 3945 4903 Size: 416 53 83 399 24 1825 703 958 Cuts at: 4903 442 Size: 1753 Fragments arranged by size: 1825 1753 958 703 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6214 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2205 2933 2205 Size: 728 5486 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2198 2387 3083 4874 1239 Size: 959 189 696 1791 2579 Fragments arranged by size: 2579 1791 959 696 189 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2399 2888 4339 5204 5502 2399 Size: 489 1451 865 298 3111 Fragments arranged by size: 3111 1451 865 489 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2403 3425 4877 882 Size: 617 904 1022 1452 2219 Fragments arranged by size: 2219 1452 1022 904 617 BsaAI yAC'GTr Cuts at: 493 1204 3334 4005 493 Size: 711 2130 671 2702 Fragments arranged by size: 2702 2130 711 671 BsaBI GATnn'nnATC Cuts at: 6208 6208 Size: 6214 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4021 4403 5038 275 Size: 53 83 186 936 2488 382 635 1451 Fragments arranged by size: 2488 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4647 5045 5319 5466 5877 4647 Size: 398 274 147 411 4984 Fragments arranged by size: 4984 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2228 2258 2713 2743 3034 Size: 30 165 30 825 30 455 30 291 Cuts at: 3034 3064 1178 Size: 30 4328 Fragments arranged by size: 4328 825 455 291 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2264 3443 1186 Size: 1078 1179 3957 Fragments arranged by size: 3957 1179 1078 BseYI C'CCAG_C Cuts at: 5417 5913 5417 Size: 496 5718 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2008 2092 2516 3039 1163 Size: 573 272 84 424 523 4338 Fragments arranged by size: 4338 573 523 424 272 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3664 4425 5453 1540 Size: 275 186 1663 761 1028 2301 Fragments arranged by size: 2301 1663 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2298 3339 3477 4275 4360 5431 2298 Size: 1041 138 798 85 1071 3081 Fragments arranged by size: 3081 1071 1041 798 138 85 BsmI GAATG_Cn' Cuts at: 2212 3725 3818 2212 Size: 1513 93 4608 Fragments arranged by size: 4608 1513 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2298 3339 3477 3533 4275 4360 5431 Size: 399 508 1041 138 56 742 85 1071 Cuts at: 5431 1391 Size: 2174 Fragments arranged by size: 2174 1071 1041 742 508 399 138 85 56 BspEI T'CCGG_A Cuts at: 5877 5877 Size: 6214 BspHI T'CATG_A Cuts at: 4103 5833 4103 Size: 1730 4484 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2030 2436 3291 3502 3569 844 Size: 1186 406 855 211 67 3489 Fragments arranged by size: 3489 1186 855 406 211 67 BsrBI CCG'CTC Cuts at: 1394 1823 4101 1394 Size: 429 2278 3507 Fragments arranged by size: 3507 2278 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4709 4883 65 Size: 1183 3461 174 1396 Fragments arranged by size: 3461 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2111 3405 4858 5045 1700 Size: 110 301 1294 1453 187 2869 Fragments arranged by size: 2869 1453 1294 301 187 110 BsrGI T'GTAC_A Cuts at: 96 2616 96 Size: 2520 3694 BssHII G'CGCG_C Cuts at: 1925 3492 1925 Size: 1567 4647 BssSI C'ACGA_G Cuts at: 2627 4274 5286 2627 Size: 1647 1012 3555 Fragments arranged by size: 3555 1647 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3472 794 Size: 2678 3536 BstBI TT'CG_AA Cuts at: 5018 5018 Size: 6214 BstEII G'GTnAC_C Cuts at: 1184 2017 1184 Size: 833 5381 BstXI CCAn_nnnn'nTGG Cuts at: 2646 2929 2646 Size: 283 5931 BstYI r'GATC_y Cuts at: 1972 2193 2361 3544 3911 4296 4313 5765 5851 Size: 221 168 1183 367 385 17 1452 86 Cuts at: 5851 5863 6209 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1183 385 367 346 221 168 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2553 2553 Size: 6214 BtgI C'CryG_G Cuts at: 513 1065 2004 2013 2813 2832 3138 513 Size: 552 939 9 800 19 306 3589 Fragments arranged by size: 3589 939 800 552 306 19 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3349 487 Size: 683 56 2123 3352 Fragments arranged by size: 3352 2123 683 56 BtsI GCAGTG_nn' Cuts at: 2466 3481 3730 4524 4544 2466 Size: 1015 249 794 20 4136 Fragments arranged by size: 4136 1015 794 249 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6214 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6179 DraI TTT'AAA Cuts at: 3518 3518 Size: 6214 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5221 809 Size: 507 3905 1802 Fragments arranged by size: 3905 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2113 2436 Size: 54 1425 50 275 18 177 106 323 Cuts at: 2436 2931 3288 3661 4550 6179 8 Size: 495 357 373 889 1629 43 Fragments arranged by size: 1629 1425 889 495 373 357 323 275 177 106 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3661 1537 Size: 275 1849 4090 Fragments arranged by size: 4090 1849 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4144 1353 Size: 2791 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4801 5175 5321 387 Size: 1064 138 3212 374 146 1280 Fragments arranged by size: 3212 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2296 2296 Size: 6214 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2198 2228 2387 2754 2825 3083 3246 Size: 393 566 30 159 367 71 258 163 Cuts at: 3246 4277 4874 5661 5929 1239 Size: 1031 597 787 268 1524 Fragments arranged by size: 1524 1031 787 597 566 393 367 268 258 163 159 71 30 EcoO109I rG'GnC_Cy Cuts at: 2557 2800 2557 Size: 243 5971 EcoRI G'AATT_C Cuts at: 3523 3901 3523 Size: 378 5836 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3062 3062 Size: 6214 FseI GG_CCGG'CC Cuts at: 3409 3409 Size: 6214 FspI TGC'GCA Cuts at: 4720 4720 Size: 6214 HaeII r_GCGC'y Cuts at: 1678 5361 1678 Size: 3683 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2082 2114 2228 2260 2690 Size: 32 135 32 510 32 114 32 430 Cuts at: 2690 2722 3034 3066 4855 4887 4929 4961 1373 Size: 32 312 32 1789 32 42 32 2626 Fragments arranged by size: 2626 1789 510 430 312 135 114 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3163 3558 3804 669 Size: 988 281 1225 395 246 3079 Fragments arranged by size: 3079 1225 988 395 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6214 HpaI GTT'AAC Cuts at: 3804 3804 Size: 6214 KpnI G_GTAC'C Cuts at: 3539 3539 Size: 6214 MfeI C'AATT_G Cuts at: 3813 3813 Size: 6214 MluI A'CGCG_T Cuts at: 6090 6090 Size: 6214 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2113 2241 5328 5512 1305 Size: 808 128 3087 184 2007 Fragments arranged by size: 3087 2007 808 184 128 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2438 2933 3290 10 Size: 54 1425 343 177 429 495 357 2934 Fragments arranged by size: 2934 1425 495 429 357 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2015 2121 2924 4172 4531 4690 518 Size: 648 849 106 803 1248 359 159 2042 Fragments arranged by size: 2042 1248 849 803 648 359 159 106 MspA1I CmG'CkG Cuts at: 1606 3508 3630 4307 5455 5700 1606 Size: 1902 122 677 1148 245 2120 Fragments arranged by size: 2120 1902 1148 677 245 122 NaeI GCC'GGC Cuts at: 3407 3407 Size: 6214 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4723 Fragments arranged by size: 4723 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6214 NgoMIV G'CCGG_C Cuts at: 3405 3405 Size: 6214 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6214 NotI GC'GGCC_GC Cuts at: 3661 3661 Size: 6214 NspI r_CATG'y Cuts at: 1132 1229 2604 3572 5117 1132 Size: 97 1375 968 1545 2229 Fragments arranged by size: 2229 1545 1375 968 97 PasI CC'CwG_GG Cuts at: 1348 3203 1348 Size: 1855 4359 PciI A'CATG_T Cuts at: 5113 5113 Size: 6214 PflMI CCAn_nnn'nTGG Cuts at: 1108 2176 2266 1108 Size: 1068 90 5056 Fragments arranged by size: 5056 1068 90 PfoI T'CCnGG_A Cuts at: 2364 2364 Size: 6214 PmeI GTTT'AAAC Cuts at: 3518 3518 Size: 6214 PmlI CAC'GTG Cuts at: 3334 3334 Size: 6214 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4283 4315 5796 5828 4283 Size: 32 1481 32 4669 Fragments arranged by size: 4669 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2557 2557 Size: 6214 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6214 PsiI TTA'TAA Cuts at: 3784 3784 Size: 6214 PstI C_TGCA'G Cuts at: 830 2461 3513 3566 830 Size: 1631 1052 53 3478 Fragments arranged by size: 3478 1631 1052 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6214 SacI G_AGCT'C Cuts at: 2298 2298 Size: 6214 SalI G'TCGA_C Cuts at: 1936 3556 1936 Size: 1620 4594 SbfI CC_TGCA'GG Cuts at: 3566 3566 Size: 6214 ScaI AGT'ACT Cuts at: 2324 4462 6174 2324 Size: 2138 1712 2364 Fragments arranged by size: 2364 2138 1712 SexAI A'CCwGG_T Cuts at: 2265 3240 2265 Size: 975 5239 SfcI C'TryA_G Cuts at: 826 1045 2326 2457 3509 3562 4697 5378 5569 Size: 219 1281 131 1052 53 1135 681 191 Cuts at: 5569 826 Size: 1471 Fragments arranged by size: 1471 1281 1135 1052 681 219 191 131 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6214 SmaI CCC'GGG Cuts at: 1872 3541 1872 Size: 1669 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2414 2903 4318 5219 5481 820 Size: 197 926 471 489 1415 901 262 1553 Fragments arranged by size: 1553 1415 926 901 489 471 262 197 SnaBI TAC'GTA Cuts at: 493 4005 493 Size: 3512 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6214 SphI G_CATG'C Cuts at: 1132 3572 1132 Size: 2440 3774 SspI AAT'ATT Cuts at: 5 52 4138 5 Size: 47 4086 2081 Fragments arranged by size: 4086 2081 47 StuI AGG'CCT Cuts at: 1517 3182 1517 Size: 1665 4549 StyI C'CwwG_G Cuts at: 513 1065 2004 3653 513 Size: 552 939 1649 3074 Fragments arranged by size: 3074 1649 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4241 4258 4411 4596 5041 325 Size: 1255 2661 17 153 185 445 1498 Fragments arranged by size: 2661 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2322 2616 3387 Size: 274 80 33 51 1443 345 294 771 Cuts at: 3387 4460 6172 96 Size: 1073 1712 138 Fragments arranged by size: 1712 1443 1073 771 345 294 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2713 2745 6174 6206 2713 Size: 32 3429 32 2721 Fragments arranged by size: 3429 2721 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2723 1316 Size: 1407 4807 XbaI T'CTAG_A Cuts at: 3550 3550 Size: 6214 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6214 XmaI C'CCGG_G Cuts at: 1870 3539 1870 Size: 1669 4545 XmnI GAAnn'nnTTC Cuts at: 2882 4343 2882 Size: 1461 4753 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4022 276 Size: 53 83 186 936 2488 2468 Fragments arranged by size: 2488 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI