(Circular) (Six-base) MAPSORT of: pFN21AB7234.seq Check: 2048 from: 1 to: 5869 pFN21AB7234 5869 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2141 2141 Size: 5869 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2963 3679 278 Size: 53 83 186 936 1427 716 2468 Fragments arranged by size: 2468 1427 936 716 186 83 53 AccI GT'mk_AC Cuts at: 1937 2928 3212 1937 Size: 991 284 4594 Fragments arranged by size: 4594 991 284 Acc65I G'GTAC_C Cuts at: 2478 3190 2478 Size: 712 5157 AclI AA'CG_TT Cuts at: 1563 3996 4369 1563 Size: 2433 373 3063 Fragments arranged by size: 3063 2433 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3932 5316 5584 1632 Size: 2300 1384 268 1917 Fragments arranged by size: 2300 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5672 AflIII A'CryG_T Cuts at: 1203 2735 4768 5745 1203 Size: 1532 2033 977 1327 Fragments arranged by size: 2033 1532 1327 977 AgeI A'CCGG_T Cuts at: 4700 4700 Size: 5869 AhdI GACnn_n'nnGTC Cuts at: 4598 4598 Size: 5869 AleI CACnn'nnGTG Cuts at: 1166 2726 1166 Size: 1560 4309 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2916 1184 Size: 1732 4137 AlwNI CAG_nnn'CTG Cuts at: 2108 2620 3612 5184 2108 Size: 512 992 1572 2793 Fragments arranged by size: 2793 1572 992 512 ApaI G_GGCC'C Cuts at: 2017 2017 Size: 5869 ApaLI G'TGCA_C Cuts at: 3926 5082 3926 Size: 1156 4713 ApoI r'AATT_y Cuts at: 1493 3158 3178 3404 3556 5665 1493 Size: 1665 20 226 152 2109 1697 Fragments arranged by size: 2109 1697 1665 226 152 20 AseI AT'TA_AT Cuts at: 160 1032 4423 160 Size: 872 3391 1606 Fragments arranged by size: 3391 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5869 AvaI C'yCGr_G Cuts at: 1870 1943 3194 1870 Size: 73 1251 4545 Fragments arranged by size: 4545 1251 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2572 2605 2572 Size: 33 5836 BamHI G'GATC_C Cuts at: 2268 3199 3566 2268 Size: 931 367 4571 Fragments arranged by size: 4571 931 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2207 2478 2864 3190 4645 Size: 325 217 627 420 271 386 326 1455 Cuts at: 4645 618 Size: 1842 Fragments arranged by size: 1842 1455 627 420 386 326 325 271 217 BanII G_rGCy'C Cuts at: 1391 2017 2433 3188 1391 Size: 626 416 755 4072 Fragments arranged by size: 4072 755 626 416 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2337 928 Size: 971 438 4460 Fragments arranged by size: 4460 971 438 BbvCI CC'TCA_GC Cuts at: 2109 2109 Size: 5869 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2921 2955 4060 4094 1903 Size: 34 984 34 1105 34 3678 Fragments arranged by size: 3678 1105 984 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2860 3761 4971 2860 Size: 901 1210 3758 Fragments arranged by size: 3758 1210 901 BclI T'GATC_A Cuts at: 1548 3012 1548 Size: 1464 4405 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4480 136 Size: 107 122 71 631 605 2808 1525 Fragments arranged by size: 2808 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5864 5864 Size: 5869 BlpI GC'TnA_GC Cuts at: 2432 3286 2432 Size: 854 5015 Bme1580I G_kGCm'C Cuts at: 1790 2017 3930 5086 1790 Size: 227 1913 1156 2573 Fragments arranged by size: 2573 1913 1156 227 BmgBI CAC'GTC Cuts at: 1318 2736 1318 Size: 1418 4451 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3600 4558 442 Size: 416 53 83 399 24 2183 958 1753 Fragments arranged by size: 2183 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5869 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2266 2846 2266 Size: 580 5289 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2312 2557 4529 1239 Size: 1073 245 1972 2579 Fragments arranged by size: 2579 1972 1073 245 Bpu10I CC'TnA_GC Cuts at: 2109 2109 Size: 5869 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2261 3994 4859 5157 2261 Size: 1733 865 298 2973 Fragments arranged by size: 2973 1733 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4532 882 Size: 617 3033 2219 Fragments arranged by size: 3033 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2653 2917 3660 493 Size: 711 1449 264 743 2702 Fragments arranged by size: 2702 1449 743 711 264 BsaBI GATnn'nnATC Cuts at: 5863 5863 Size: 5869 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2882 2960 3676 4058 Size: 53 83 186 936 1349 78 716 382 Cuts at: 4058 4693 275 Size: 635 1451 Fragments arranged by size: 1451 1349 936 716 635 382 186 83 78 53 BsaWI w'CCGG_w Cuts at: 2627 4302 4700 4974 5121 5532 2627 Size: 1675 398 274 147 411 2964 Fragments arranged by size: 2964 1675 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5644 Fragments arranged by size: 5644 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2258 1186 Size: 1072 4797 BseYI C'CCAG_C Cuts at: 2179 5072 5568 2179 Size: 2893 496 2480 Fragments arranged by size: 2893 2480 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2126 2684 1163 Size: 573 390 558 4348 Fragments arranged by size: 4348 573 558 390 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2055 3319 4080 5108 1540 Size: 275 186 54 1264 761 1028 2301 Fragments arranged by size: 2301 1264 1028 761 275 186 54 BsiHKAI G_wGCw'C Cuts at: 2433 3047 3930 4015 5086 2433 Size: 614 883 85 1071 3216 Fragments arranged by size: 3216 1071 883 614 85 BsmI GAATG_Cn' Cuts at: 3380 3473 3380 Size: 93 5776 BsmBI CGTCTCn'nnnn_ Cuts at: 2819 2819 Size: 5869 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2017 2433 3047 3188 3930 4015 5086 Size: 399 227 416 614 141 742 85 1071 Cuts at: 5086 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 614 416 399 227 141 85 BspEI T'CCGG_A Cuts at: 2627 5532 2627 Size: 2905 2964 BspHI T'CATG_A Cuts at: 3758 5488 3758 Size: 1730 4139 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2141 3224 844 Size: 1297 1083 3489 Fragments arranged by size: 3489 1297 1083 BsrBI CCG'CTC Cuts at: 1394 1823 3756 1394 Size: 429 1933 3507 Fragments arranged by size: 3507 1933 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4364 4538 65 Size: 1183 3116 174 1396 Fragments arranged by size: 3116 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4513 4700 1700 Size: 110 2703 187 2869 Fragments arranged by size: 2869 2703 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5869 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5869 BssSI C'ACGA_G Cuts at: 3929 4941 3929 Size: 1012 4857 BstAPI GCAn_nnn'nTGC Cuts at: 794 2653 794 Size: 1859 4010 BstBI TT'CG_AA Cuts at: 4673 4673 Size: 5869 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5869 BstXI CCAn_nnnn'nTGG Cuts at: 2153 2525 3091 2153 Size: 372 566 4931 Fragments arranged by size: 4931 566 372 BstYI r'GATC_y Cuts at: 1972 2268 2769 3199 3566 3951 3968 5420 5506 Size: 296 501 430 367 385 17 1452 86 Cuts at: 5506 5518 5864 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 501 430 385 367 346 296 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2055 2447 2676 2782 2817 2853 Size: 552 939 51 392 229 106 35 36 Cuts at: 2853 513 Size: 3529 Fragments arranged by size: 3529 939 552 392 229 106 51 36 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5130 Fragments arranged by size: 5130 683 56 BtsI GCAGTG_nn' Cuts at: 3385 4179 4199 3385 Size: 794 20 5055 Fragments arranged by size: 5055 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5869 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5834 DraI TTT'AAA Cuts at: 3173 3173 Size: 5869 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4876 809 Size: 507 3560 1802 Fragments arranged by size: 3560 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2030 2052 2896 Size: 54 1425 50 275 18 200 22 844 Cuts at: 2896 3316 4205 5834 8 Size: 420 889 1629 43 Fragments arranged by size: 1629 1425 889 844 420 275 200 54 50 43 22 18 EagI C'GGCC_G Cuts at: 1537 1812 2052 3316 1537 Size: 275 240 1264 4090 Fragments arranged by size: 4090 1264 275 240 EarI CTCTTCn'nnn_ Cuts at: 1353 2201 3799 1353 Size: 848 1598 3423 Fragments arranged by size: 3423 1598 848 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2825 4456 4830 4976 387 Size: 1064 138 1236 1631 374 146 1280 Fragments arranged by size: 1631 1280 1236 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2431 2431 Size: 5869 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2312 2557 3932 4529 5316 5584 1239 Size: 393 680 245 1375 597 787 268 1524 Fragments arranged by size: 1524 1375 787 680 597 393 268 245 EcoRI G'AATT_C Cuts at: 3158 3178 3556 3158 Size: 20 378 5471 Fragments arranged by size: 5471 378 20 FspI TGC'GCA Cuts at: 4375 4375 Size: 5869 HaeII r_GCGC'y Cuts at: 1678 2023 2621 2912 2999 5016 1678 Size: 345 598 291 87 2017 2531 Fragments arranged by size: 2531 2017 598 345 291 87 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4510 4542 4584 4616 1373 Size: 32 135 32 2938 32 42 32 2626 Fragments arranged by size: 2938 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2733 3213 3459 669 Size: 988 281 795 480 246 3079 Fragments arranged by size: 3079 988 795 480 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5869 HpaI GTT'AAC Cuts at: 3459 3459 Size: 5869 KpnI G_GTAC'C Cuts at: 2482 3194 2482 Size: 712 5157 MfeI C'AATT_G Cuts at: 3468 3468 Size: 5869 MluI A'CGCG_T Cuts at: 5745 5745 Size: 5869 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4983 5167 1305 Size: 3678 184 2007 Fragments arranged by size: 3678 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4047 Fragments arranged by size: 4047 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2148 2726 2898 3020 3827 4186 4345 Size: 648 982 578 172 122 807 359 159 Cuts at: 4345 518 Size: 2042 Fragments arranged by size: 2042 982 807 648 578 359 172 159 122 MspA1I CmG'CkG Cuts at: 1606 2035 2057 2436 2449 2775 2784 2855 3285 Size: 429 22 379 13 326 9 71 430 Cuts at: 3285 3962 5110 5355 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 430 429 379 326 245 71 22 13 9 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4378 Fragments arranged by size: 4378 939 552 NdeI CA'TA_TG Cuts at: 387 3017 387 Size: 2630 3239 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5869 NotI GC'GGCC_GC Cuts at: 2052 3316 2052 Size: 1264 4605 NspI r_CATG'y Cuts at: 1132 1229 3227 4772 1132 Size: 97 1998 1545 2229 Fragments arranged by size: 2229 1998 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5869 PciI A'CATG_T Cuts at: 4768 4768 Size: 5869 PflMI CCAn_nnn'nTGG Cuts at: 1108 2704 1108 Size: 1596 4273 PfoI T'CCnGG_A Cuts at: 2397 2397 Size: 5869 PmeI GTTT'AAAC Cuts at: 3173 3173 Size: 5869 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3938 3970 5451 5483 3938 Size: 32 1481 32 4324 Fragments arranged by size: 4324 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5869 PsiI TTA'TAA Cuts at: 3439 3439 Size: 5869 PspOMI G'GGCC_C Cuts at: 2013 2013 Size: 5869 PstI C_TGCA'G Cuts at: 830 3221 830 Size: 2391 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5869 PvuII CAG'CTG Cuts at: 2436 2436 Size: 5869 SacI G_AGCT'C Cuts at: 2433 2433 Size: 5869 SacII CC_GC'GG Cuts at: 2058 2450 2785 2856 2058 Size: 392 335 71 5071 Fragments arranged by size: 5071 392 335 71 SalI G'TCGA_C Cuts at: 1936 3211 1936 Size: 1275 4594 SbfI CC_TGCA'GG Cuts at: 3221 3221 Size: 5869 ScaI AGT'ACT Cuts at: 2669 4117 5829 2669 Size: 1448 1712 2709 Fragments arranged by size: 2709 1712 1448 SfcI C'TryA_G Cuts at: 826 1045 3217 4352 5033 5224 826 Size: 219 2172 1135 681 191 1471 Fragments arranged by size: 2172 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5869 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5869 SmaI CCC'GGG Cuts at: 1872 3196 1872 Size: 1324 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2240 3973 4874 5136 820 Size: 197 926 297 1733 901 262 1553 Fragments arranged by size: 1733 1553 926 901 297 262 197 SnaBI TAC'GTA Cuts at: 493 2917 3660 493 Size: 2424 743 2702 Fragments arranged by size: 2702 2424 743 SpeI A'CTAG_T Cuts at: 152 2365 152 Size: 2213 3656 SphI G_CATG'C Cuts at: 1132 3227 1132 Size: 2095 3774 SspI AAT'ATT Cuts at: 5 52 3793 5 Size: 47 3741 2081 Fragments arranged by size: 3741 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5869 StyI C'CwwG_G Cuts at: 513 1065 2004 3308 513 Size: 552 939 1304 3074 Fragments arranged by size: 3074 1304 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2351 3896 3913 4066 4251 4696 325 Size: 1255 771 1545 17 153 185 445 1498 Fragments arranged by size: 1545 1498 1255 771 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2083 2667 4115 Size: 274 80 33 51 1443 106 584 1448 Cuts at: 4115 5827 96 Size: 1712 138 Fragments arranged by size: 1712 1448 1443 584 274 138 106 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5829 5861 5829 Size: 32 5837 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5869 XbaI T'CTAG_A Cuts at: 3205 3205 Size: 5869 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3083 3083 Size: 5869 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5869 XmaI C'CCGG_G Cuts at: 1870 3194 1870 Size: 1324 4545 XmnI GAAnn'nnTTC Cuts at: 3998 3998 Size: 5869 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2961 3677 276 Size: 53 83 186 936 1427 716 2468 Fragments arranged by size: 2468 1427 936 716 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI