(Circular) (Six-base) MAPSORT of: pFN21AE0491.seq Check: 6976 from: 1 to: 5674 pFN21AE0491 5674 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3484 278 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3017 1937 Size: 1080 4594 Acc65I G'GTAC_C Cuts at: 2995 2995 Size: 5674 AclI AA'CG_TT Cuts at: 1563 3801 4174 1563 Size: 2238 373 3063 Fragments arranged by size: 3063 2238 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2050 2858 3737 5121 5389 1632 Size: 418 808 879 1384 268 1917 Fragments arranged by size: 1917 1384 879 808 418 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5477 AflIII A'CryG_T Cuts at: 1203 2601 4573 5550 1203 Size: 1398 1972 977 1327 Fragments arranged by size: 1972 1398 1327 977 AgeI A'CCGG_T Cuts at: 4505 4505 Size: 5674 AhdI GACnn_n'nnGTC Cuts at: 4403 4403 Size: 5674 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5674 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5674 AlwNI CAG_nnn'CTG Cuts at: 3417 4989 3417 Size: 1572 4102 ApaI G_GGCC'C Cuts at: 2557 2557 Size: 5674 ApaLI G'TGCA_C Cuts at: 2597 3731 4887 2597 Size: 1134 1156 3384 Fragments arranged by size: 3384 1156 1134 ApoI r'AATT_y Cuts at: 1493 2218 2983 3209 3361 5470 1493 Size: 725 765 226 152 2109 1697 Fragments arranged by size: 2109 1697 765 725 226 152 AseI AT'TA_AT Cuts at: 160 1032 4228 160 Size: 872 3196 1606 Fragments arranged by size: 3196 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 AvaI C'yCGr_G Cuts at: 1870 1943 2999 1870 Size: 73 1056 4545 Fragments arranged by size: 4545 1056 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2893 2926 2893 Size: 33 5641 BamHI G'GATC_C Cuts at: 3004 3371 3004 Size: 367 5307 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2995 4450 618 Size: 325 217 627 1208 1455 1842 Fragments arranged by size: 1842 1455 1208 627 325 217 BanII G_rGCy'C Cuts at: 1391 2557 2742 2993 1391 Size: 1166 185 251 4072 Fragments arranged by size: 4072 1166 251 185 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4703 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2410 2444 3865 3899 1903 Size: 34 473 34 1421 34 3678 Fragments arranged by size: 3678 1421 473 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2921 3566 4776 2921 Size: 645 1210 3819 Fragments arranged by size: 3819 1210 645 BclI T'GATC_A Cuts at: 1548 2245 1548 Size: 697 4977 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2309 4285 136 Size: 107 122 71 631 605 637 1976 1525 Fragments arranged by size: 1976 1525 637 631 605 122 107 71 BglII A'GATC_T Cuts at: 2805 5669 2805 Size: 2864 2810 BlpI GC'TnA_GC Cuts at: 2822 3091 2822 Size: 269 5405 Bme1580I G_kGCm'C Cuts at: 1790 2557 2601 3735 4891 1790 Size: 767 44 1134 1156 2573 Fragments arranged by size: 2573 1156 1134 767 44 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5674 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3405 4363 442 Size: 416 53 83 399 24 1988 958 1753 Fragments arranged by size: 1988 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5674 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2095 2299 2642 2704 2734 2879 4334 1239 Size: 856 204 343 62 30 145 1455 2579 Fragments arranged by size: 2579 1455 856 343 204 145 62 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3799 4664 4962 3799 Size: 865 298 4511 Fragments arranged by size: 4511 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2484 4337 882 Size: 617 985 1853 2219 Fragments arranged by size: 2219 1853 985 617 BsaAI yAC'GTr Cuts at: 493 1204 3465 493 Size: 711 2261 2702 Fragments arranged by size: 2702 2261 711 BsaBI GATnn'nnATC Cuts at: 5668 5668 Size: 5674 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2757 3481 3863 4498 Size: 53 83 186 936 1224 724 382 635 Cuts at: 4498 275 Size: 1451 Fragments arranged by size: 1451 1224 936 724 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2186 4107 4505 4779 4926 5337 2186 Size: 1921 398 274 147 411 2523 Fragments arranged by size: 2523 1921 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5449 Fragments arranged by size: 5449 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5674 BseYI C'CCAG_C Cuts at: 2684 4877 5373 2684 Size: 2193 496 2985 Fragments arranged by size: 2985 2193 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5101 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2614 3124 3885 4913 1540 Size: 275 186 613 510 761 1028 2301 Fragments arranged by size: 2301 1028 761 613 510 275 186 BsiHKAI G_wGCw'C Cuts at: 2601 3735 3820 4891 2601 Size: 1134 85 1071 3384 Fragments arranged by size: 3384 1134 1071 85 BsmI GAATG_Cn' Cuts at: 3185 3278 3185 Size: 93 5581 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2557 2601 2742 2993 3735 3820 4891 Size: 399 767 44 141 251 742 85 1071 Cuts at: 4891 1391 Size: 2174 Fragments arranged by size: 2174 1071 767 742 399 251 141 85 44 BspEI T'CCGG_A Cuts at: 2186 5337 2186 Size: 3151 2523 BspHI T'CATG_A Cuts at: 3563 5293 3563 Size: 1730 3944 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2210 2429 3029 844 Size: 1366 219 600 3489 Fragments arranged by size: 3489 1366 600 219 BsrBI CCG'CTC Cuts at: 1394 1823 2718 3561 1394 Size: 429 895 843 3507 Fragments arranged by size: 3507 895 843 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4169 4343 65 Size: 1183 2921 174 1396 Fragments arranged by size: 2921 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2417 2613 4318 4505 1700 Size: 110 607 196 1705 187 2869 Fragments arranged by size: 2869 1705 607 196 187 110 BsrGI T'GTAC_A Cuts at: 96 2469 2814 96 Size: 2373 345 2956 Fragments arranged by size: 2956 2373 345 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5674 BssSI C'ACGA_G Cuts at: 3734 4746 3734 Size: 1012 4662 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5674 BstBI TT'CG_AA Cuts at: 4478 4478 Size: 5674 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5674 BstXI CCAn_nnnn'nTGG Cuts at: 2086 2086 Size: 5674 BstYI r'GATC_y Cuts at: 1972 2805 3004 3371 3756 3773 5225 5311 5323 Size: 833 199 367 385 17 1452 86 12 Cuts at: 5323 5669 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 833 385 367 346 199 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2634 2646 2733 2766 2799 513 Size: 552 939 630 12 87 33 33 3388 Fragments arranged by size: 3388 939 630 552 87 33 33 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2353 2554 487 Size: 683 56 1127 201 3607 Fragments arranged by size: 3607 1127 683 201 56 BtsI GCAGTG_nn' Cuts at: 3190 3984 4004 3190 Size: 794 20 4860 Fragments arranged by size: 4860 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5674 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5639 DraI TTT'AAA Cuts at: 2978 2978 Size: 5674 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4681 809 Size: 507 3365 1802 Fragments arranged by size: 3365 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3121 4010 5639 Size: 54 1425 50 275 18 1291 889 1629 Cuts at: 5639 8 Size: 43 Fragments arranged by size: 1629 1425 1291 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3121 1537 Size: 275 1309 4090 Fragments arranged by size: 4090 1309 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3604 1353 Size: 2251 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4261 4635 4781 387 Size: 1064 138 2672 374 146 1280 Fragments arranged by size: 2672 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2050 2095 2299 2642 2704 2734 2858 Size: 393 418 45 204 343 62 30 124 Cuts at: 2858 2879 3737 4334 5121 5389 1239 Size: 21 858 597 787 268 1524 Fragments arranged by size: 1524 858 787 597 418 393 343 268 204 124 62 45 30 21 EcoO109I rG'GnC_Cy Cuts at: 2310 2553 2554 2830 2310 Size: 243 1 276 5154 Fragments arranged by size: 5154 276 243 1 EcoRI G'AATT_C Cuts at: 2983 3361 2983 Size: 378 5296 FspI TGC'GCA Cuts at: 4180 4180 Size: 5674 HaeII r_GCGC'y Cuts at: 1678 2710 4821 1678 Size: 1032 2111 2531 Fragments arranged by size: 2531 2111 1032 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2388 2420 4315 4347 4389 Size: 32 135 32 816 32 1895 32 42 Cuts at: 4389 4421 1373 Size: 32 2626 Fragments arranged by size: 2626 1895 816 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3018 3264 669 Size: 988 281 1080 246 3079 Fragments arranged by size: 3079 1080 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5674 HpaI GTT'AAC Cuts at: 3264 3264 Size: 5674 KpnI G_GTAC'C Cuts at: 2999 2999 Size: 5674 MfeI C'AATT_G Cuts at: 3273 3273 Size: 5674 MluI A'CGCG_T Cuts at: 5550 5550 Size: 5674 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2898 2967 4788 4972 1305 Size: 1593 69 1821 184 2007 Fragments arranged by size: 2007 1821 1593 184 69 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3852 Fragments arranged by size: 3852 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2084 2465 3632 3991 4150 518 Size: 648 918 381 1167 359 159 2042 Fragments arranged by size: 2042 1167 918 648 381 359 159 MspA1I CmG'CkG Cuts at: 1606 2626 3090 3767 4915 5160 1606 Size: 1020 464 677 1148 245 2120 Fragments arranged by size: 2120 1148 1020 677 464 245 NaeI GCC'GGC Cuts at: 2419 2419 Size: 5674 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4183 Fragments arranged by size: 4183 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5674 NgoMIV G'CCGG_C Cuts at: 2417 2417 Size: 5674 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5674 NotI GC'GGCC_GC Cuts at: 3121 3121 Size: 5674 NspI r_CATG'y Cuts at: 1132 1229 2605 3032 4577 1132 Size: 97 1376 427 1545 2229 Fragments arranged by size: 2229 1545 1376 427 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5674 PciI A'CATG_T Cuts at: 2601 4573 2601 Size: 1972 3702 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5674 PmeI GTTT'AAAC Cuts at: 2978 2978 Size: 5674 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2915 2947 3743 3775 5256 5288 2915 Size: 32 796 32 1481 32 3301 Fragments arranged by size: 3301 1481 796 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2830 2830 Size: 5674 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5674 PsiI TTA'TAA Cuts at: 3244 3244 Size: 5674 PspOMI G'GGCC_C Cuts at: 2553 2553 Size: 5674 PstI C_TGCA'G Cuts at: 830 2440 3026 830 Size: 1610 586 3478 Fragments arranged by size: 3478 1610 586 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5674 PvuII CAG'CTG Cuts at: 2626 2626 Size: 5674 SalI G'TCGA_C Cuts at: 1936 3016 1936 Size: 1080 4594 SanDI GG'GwC_CC Cuts at: 2830 2830 Size: 5674 SbfI CC_TGCA'GG Cuts at: 3026 3026 Size: 5674 ScaI AGT'ACT Cuts at: 3922 5634 3922 Size: 1712 3962 SfcI C'TryA_G Cuts at: 826 1045 2436 3022 4157 4838 5029 826 Size: 219 1391 586 1135 681 191 1471 Fragments arranged by size: 1471 1391 1135 681 586 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2309 2309 Size: 5674 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5674 SmaI CCC'GGG Cuts at: 1872 3001 1872 Size: 1129 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3778 4679 4941 820 Size: 197 926 1835 901 262 1553 Fragments arranged by size: 1835 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3465 493 Size: 2972 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5674 SphI G_CATG'C Cuts at: 1132 3032 1132 Size: 1900 3774 SspI AAT'ATT Cuts at: 5 52 2291 3598 5 Size: 47 2239 1307 2081 Fragments arranged by size: 2239 2081 1307 47 StuI AGG'CCT Cuts at: 1517 2663 1517 Size: 1146 4528 StyI C'CwwG_G Cuts at: 513 1065 2004 3113 513 Size: 552 939 1109 3074 Fragments arranged by size: 3074 1109 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2345 3701 3718 3871 4056 4501 325 Size: 1255 765 1356 17 153 185 445 1498 Fragments arranged by size: 1498 1356 1255 765 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2469 2814 3920 Size: 274 80 33 51 1443 492 345 1106 Cuts at: 3920 5632 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1106 492 345 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5634 5666 5634 Size: 32 5642 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5674 XbaI T'CTAG_A Cuts at: 3010 3010 Size: 5674 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5674 XmaI C'CCGG_G Cuts at: 1870 2999 1870 Size: 1129 4545 XmnI GAAnn'nnTTC Cuts at: 3803 3803 Size: 5674 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3482 276 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfoI SrfI SwaI XcmI