(Circular) (Six-base) MAPSORT of: pFN21AB8867.seq Check: 5667 from: 1 to: 5071 pFN21AB8867 5071 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2059 2188 2059 Size: 129 4942 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2881 278 Size: 53 83 186 936 1345 2468 Fragments arranged by size: 2468 1345 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2414 1937 Size: 477 4594 Acc65I G'GTAC_C Cuts at: 2392 2392 Size: 5071 AclI AA'CG_TT Cuts at: 1563 3198 3571 1563 Size: 1635 373 3063 Fragments arranged by size: 3063 1635 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3134 4518 4786 1632 Size: 1502 1384 268 1917 Fragments arranged by size: 1917 1502 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4874 AflIII A'CryG_T Cuts at: 1203 3970 4947 1203 Size: 2767 977 1327 Fragments arranged by size: 2767 1327 977 AgeI A'CCGG_T Cuts at: 3902 3902 Size: 5071 AhdI GACnn_n'nnGTC Cuts at: 2233 3800 2233 Size: 1567 3504 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5071 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5071 AlwNI CAG_nnn'CTG Cuts at: 2280 2814 4386 2280 Size: 534 1572 2965 Fragments arranged by size: 2965 1572 534 ApaLI G'TGCA_C Cuts at: 3128 4284 3128 Size: 1156 3915 ApoI r'AATT_y Cuts at: 1493 2380 2606 2758 4867 1493 Size: 887 226 152 2109 1697 Fragments arranged by size: 2109 1697 887 226 152 AseI AT'TA_AT Cuts at: 160 1032 3625 160 Size: 872 2593 1606 Fragments arranged by size: 2593 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5071 AvaI C'yCGr_G Cuts at: 1870 1943 2396 1870 Size: 73 453 4545 Fragments arranged by size: 4545 453 73 BamHI G'GATC_C Cuts at: 2401 2768 2401 Size: 367 4704 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2392 3847 618 Size: 325 217 627 605 1455 1842 Fragments arranged by size: 1842 1455 627 605 325 217 BanII G_rGCy'C Cuts at: 1391 2093 2390 1391 Size: 702 297 4072 Fragments arranged by size: 4072 702 297 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2232 928 Size: 971 333 3767 Fragments arranged by size: 3767 971 333 BbvCI CC'TCA_GC Cuts at: 2273 2273 Size: 5071 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3262 3296 1903 Size: 34 50 34 1241 34 3678 Fragments arranged by size: 3678 1241 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2963 4173 2963 Size: 1210 3861 BclI T'GATC_A Cuts at: 1548 1548 Size: 5071 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2326 3682 136 Size: 107 122 71 631 605 654 1356 1525 Fragments arranged by size: 1525 1356 654 631 605 122 107 71 BglII A'GATC_T Cuts at: 5066 5066 Size: 5071 BlpI GC'TnA_GC Cuts at: 2488 2488 Size: 5071 Bme1580I G_kGCm'C Cuts at: 1790 3132 4288 1790 Size: 1342 1156 2573 Fragments arranged by size: 2573 1342 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5071 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2802 3760 442 Size: 416 53 83 399 24 1385 958 1753 Fragments arranged by size: 1753 1385 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5071 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3731 1239 Size: 2492 2579 Bpu10I CC'TnA_GC Cuts at: 2273 2273 Size: 5071 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3196 4061 4359 3196 Size: 865 298 3908 Fragments arranged by size: 3908 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3734 882 Size: 617 2235 2219 Fragments arranged by size: 2235 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2862 493 Size: 711 1658 2702 Fragments arranged by size: 2702 1658 711 BsaBI GATnn'nnATC Cuts at: 5065 5065 Size: 5071 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2878 3260 3895 275 Size: 53 83 186 936 1345 382 635 1451 Fragments arranged by size: 1451 1345 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3504 3902 4176 4323 4734 3504 Size: 398 274 147 411 3841 Fragments arranged by size: 3841 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4846 Fragments arranged by size: 4846 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2167 1186 Size: 981 4090 BseYI C'CCAG_C Cuts at: 2022 2214 4274 4770 2022 Size: 192 2060 496 2323 Fragments arranged by size: 2323 2060 496 192 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4498 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2521 3282 4310 1540 Size: 275 186 520 761 1028 2301 Fragments arranged by size: 2301 1028 761 520 275 186 BsiHKAI G_wGCw'C Cuts at: 2166 3132 3217 4288 2166 Size: 966 85 1071 2949 Fragments arranged by size: 2949 1071 966 85 BsmI GAATG_Cn' Cuts at: 2582 2675 2582 Size: 93 4978 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2093 2166 2390 3132 3217 4288 1391 Size: 399 303 73 224 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 303 224 85 73 BspEI T'CCGG_A Cuts at: 4734 4734 Size: 5071 BspHI T'CATG_A Cuts at: 2960 4690 2960 Size: 1730 3341 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2059 2188 2426 844 Size: 1215 129 238 3489 Fragments arranged by size: 3489 1215 238 129 BsrBI CCG'CTC Cuts at: 1394 1823 2958 1394 Size: 429 1135 3507 Fragments arranged by size: 3507 1135 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3566 3740 65 Size: 1183 2318 174 1396 Fragments arranged by size: 2318 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3715 3902 1700 Size: 110 1905 187 2869 Fragments arranged by size: 2869 1905 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5071 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5071 BssSI C'ACGA_G Cuts at: 3131 4143 3131 Size: 1012 4059 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5071 BstBI TT'CG_AA Cuts at: 2157 3875 2157 Size: 1718 3353 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5071 BstXI CCAn_nnnn'nTGG Cuts at: 2302 2302 Size: 5071 BstYI r'GATC_y Cuts at: 1972 2401 2768 3153 3170 4622 4708 4720 5066 Size: 429 367 385 17 1452 86 12 346 Cuts at: 5066 1972 Size: 1977 Fragments arranged by size: 1977 1452 429 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4519 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4332 Fragments arranged by size: 4332 683 56 BtsI GCAGTG_nn' Cuts at: 2043 2587 3381 3401 2043 Size: 544 794 20 3713 Fragments arranged by size: 3713 794 544 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5071 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5036 DraI TTT'AAA Cuts at: 2375 2375 Size: 5071 DraIII CAC_nnn'GTG Cuts at: 2170 2170 Size: 5071 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4078 809 Size: 507 2762 1802 Fragments arranged by size: 2762 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2292 2518 3407 Size: 54 1425 50 275 18 462 226 889 Cuts at: 3407 5036 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 462 275 226 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2518 1537 Size: 275 706 4090 Fragments arranged by size: 4090 706 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2154 3001 1353 Size: 801 847 3423 Fragments arranged by size: 3423 847 801 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2344 3658 4032 4178 387 Size: 1064 138 755 1314 374 146 1280 Fragments arranged by size: 1314 1280 1064 755 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3134 3731 4518 4786 1239 Size: 393 1502 597 787 268 1524 Fragments arranged by size: 1524 1502 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2096 2096 Size: 5071 EcoRI G'AATT_C Cuts at: 2380 2758 2380 Size: 378 4693 FspI TGC'GCA Cuts at: 3577 3577 Size: 5071 HaeII r_GCGC'y Cuts at: 1678 4218 1678 Size: 2540 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2219 2251 3712 3744 3786 Size: 32 135 32 647 32 1461 32 42 Cuts at: 3786 3818 1373 Size: 32 2626 Fragments arranged by size: 2626 1461 647 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2415 2661 669 Size: 988 281 477 246 3079 Fragments arranged by size: 3079 988 477 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5071 HpaI GTT'AAC Cuts at: 2661 2661 Size: 5071 KpnI G_GTAC'C Cuts at: 2396 2396 Size: 5071 MfeI C'AATT_G Cuts at: 2670 2670 Size: 5071 MluI A'CGCG_T Cuts at: 4947 4947 Size: 5071 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2371 4185 4369 1305 Size: 1066 1814 184 2007 Fragments arranged by size: 2007 1814 1066 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2294 10 Size: 54 1425 343 462 2787 Fragments arranged by size: 2787 1425 462 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3029 3388 3547 518 Size: 648 1863 359 159 2042 Fragments arranged by size: 2042 1863 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2249 2487 3164 4312 4557 1606 Size: 643 238 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 643 245 238 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4519 NdeI CA'TA_TG Cuts at: 387 387 Size: 5071 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5071 NotI GC'GGCC_GC Cuts at: 2518 2518 Size: 5071 NspI r_CATG'y Cuts at: 1132 1229 2429 3974 1132 Size: 97 1200 1545 2229 Fragments arranged by size: 2229 1545 1200 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5071 PciI A'CATG_T Cuts at: 3970 3970 Size: 5071 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5071 PmeI GTTT'AAAC Cuts at: 2375 2375 Size: 5071 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3140 3172 4653 4685 3140 Size: 32 1481 32 3526 Fragments arranged by size: 3526 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5071 PsiI TTA'TAA Cuts at: 2641 2641 Size: 5071 PstI C_TGCA'G Cuts at: 830 2033 2070 2137 2423 830 Size: 1203 37 67 286 3478 Fragments arranged by size: 3478 1203 286 67 37 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5071 PvuII CAG'CTG Cuts at: 2249 2249 Size: 5071 SalI G'TCGA_C Cuts at: 1936 2413 1936 Size: 477 4594 SapI GCTCTTCn'nnn_ Cuts at: 2154 2154 Size: 5071 SbfI CC_TGCA'GG Cuts at: 2423 2423 Size: 5071 ScaI AGT'ACT Cuts at: 3319 5031 3319 Size: 1712 3359 SfcI C'TryA_G Cuts at: 826 1045 2029 2066 2133 2419 3554 4235 4426 Size: 219 984 37 67 286 1135 681 191 Cuts at: 4426 826 Size: 1471 Fragments arranged by size: 1471 1135 984 681 286 219 191 67 37 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5071 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5071 SmaI CCC'GGG Cuts at: 1872 2398 1872 Size: 526 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3175 4076 4338 820 Size: 197 926 1232 901 262 1553 Fragments arranged by size: 1553 1232 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2862 493 Size: 2369 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5071 SphI G_CATG'C Cuts at: 1132 2429 1132 Size: 1297 3774 SspI AAT'ATT Cuts at: 5 52 2995 5 Size: 47 2943 2081 Fragments arranged by size: 2943 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5071 StyI C'CwwG_G Cuts at: 513 1065 2510 513 Size: 552 1445 3074 Fragments arranged by size: 3074 1445 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2174 3098 3115 3268 3453 3898 325 Size: 1255 594 924 17 153 185 445 1498 Fragments arranged by size: 1498 1255 924 594 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3317 5029 96 Size: 274 80 33 51 1443 1340 1712 138 Fragments arranged by size: 1712 1443 1340 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5031 5063 5031 Size: 32 5039 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5071 XbaI T'CTAG_A Cuts at: 2407 2407 Size: 5071 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5071 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5071 XmaI C'CCGG_G Cuts at: 1870 2396 1870 Size: 526 4545 XmnI GAAnn'nnTTC Cuts at: 3200 3200 Size: 5071 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2879 276 Size: 53 83 186 936 1345 2468 Fragments arranged by size: 2468 1345 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI