(Circular) (Six-base) MAPSORT of: pFN21AE0521.seq Check: 9612 from: 1 to: 5533 pFN21AE0521 5533 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2403 3343 278 Size: 53 83 186 936 867 940 2468 Fragments arranged by size: 2468 940 936 867 186 83 53 AccI GT'mk_AC Cuts at: 1937 2876 1937 Size: 939 4594 Acc65I G'GTAC_C Cuts at: 2854 2854 Size: 5533 AclI AA'CG_TT Cuts at: 1563 3660 4033 1563 Size: 2097 373 3063 Fragments arranged by size: 3063 2097 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2342 3596 4980 5248 1632 Size: 710 1254 1384 268 1917 Fragments arranged by size: 1917 1384 1254 710 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5336 AflIII A'CryG_T Cuts at: 1203 2052 2201 4432 5409 1203 Size: 849 149 2231 977 1327 Fragments arranged by size: 2231 1327 977 849 149 AgeI A'CCGG_T Cuts at: 4364 4364 Size: 5533 AhdI GACnn_n'nnGTC Cuts at: 2311 4262 2311 Size: 1951 3582 AleI CACnn'nnGTG Cuts at: 1166 2300 1166 Size: 1134 4399 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2495 1184 Size: 1311 4222 AlwNI CAG_nnn'CTG Cuts at: 2554 3276 4848 2554 Size: 722 1572 3239 Fragments arranged by size: 3239 1572 722 ApaLI G'TGCA_C Cuts at: 3590 4746 3590 Size: 1156 4377 ApoI r'AATT_y Cuts at: 1493 2087 2842 3068 3220 5329 1493 Size: 594 755 226 152 2109 1697 Fragments arranged by size: 2109 1697 755 594 226 152 AseI AT'TA_AT Cuts at: 160 1032 4087 160 Size: 872 3055 1606 Fragments arranged by size: 3055 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5533 AvaI C'yCGr_G Cuts at: 1870 1943 2259 2858 1870 Size: 73 316 599 4545 Fragments arranged by size: 4545 599 316 73 BamHI G'GATC_C Cuts at: 2863 3230 2863 Size: 367 5166 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2456 2708 2854 4309 618 Size: 325 217 627 669 252 146 1455 1842 Fragments arranged by size: 1842 1455 669 627 325 252 217 146 BanII G_rGCy'C Cuts at: 1391 2852 1391 Size: 1461 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4562 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3724 3758 1903 Size: 34 1787 34 3678 Fragments arranged by size: 3678 1787 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3425 4635 3425 Size: 1210 4323 BclI T'GATC_A Cuts at: 1548 1548 Size: 5533 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4144 136 Size: 107 122 71 631 605 2472 1525 Fragments arranged by size: 2472 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5528 5528 Size: 5533 BlpI GC'TnA_GC Cuts at: 2468 2666 2950 2468 Size: 198 284 5051 Fragments arranged by size: 5051 284 198 Bme1580I G_kGCm'C Cuts at: 1790 3594 4750 1790 Size: 1804 1156 2573 Fragments arranged by size: 2573 1804 1156 BmgBI CAC'GTC Cuts at: 1318 2302 1318 Size: 984 4549 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2806 3264 4222 Size: 416 53 83 399 24 1389 458 958 Cuts at: 4222 442 Size: 1753 Fragments arranged by size: 1753 1389 958 458 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5533 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2504 2515 2549 4193 1239 Size: 1265 11 34 1644 2579 Fragments arranged by size: 2579 1644 1265 34 11 Bpu10I CC'TnA_GC Cuts at: 2381 2381 Size: 5533 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3658 4523 4821 3658 Size: 865 298 4370 Fragments arranged by size: 4370 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2335 2795 4196 882 Size: 617 836 460 1401 2219 Fragments arranged by size: 2219 1401 836 617 460 BsaAI yAC'GTr Cuts at: 493 1204 2204 2653 2752 3324 493 Size: 711 1000 449 99 572 2702 Fragments arranged by size: 2702 1000 711 572 449 99 BsaBI GATnn'nnATC Cuts at: 5527 5527 Size: 5533 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2400 2450 3340 3722 Size: 53 83 186 936 867 50 890 382 Cuts at: 3722 4357 275 Size: 635 1451 Fragments arranged by size: 1451 936 890 867 635 382 186 83 53 50 BsaWI w'CCGG_w Cuts at: 3966 4364 4638 4785 5196 3966 Size: 398 274 147 411 4303 Fragments arranged by size: 4303 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5308 Fragments arranged by size: 5308 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5533 BseYI C'CCAG_C Cuts at: 2188 4736 5232 2188 Size: 2548 496 2489 Fragments arranged by size: 2548 2489 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2675 1163 Size: 573 939 4021 Fragments arranged by size: 4021 939 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2983 3744 4772 1540 Size: 275 186 982 761 1028 2301 Fragments arranged by size: 2301 1028 982 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2387 2571 2595 3594 3679 4750 2387 Size: 184 24 999 85 1071 3170 Fragments arranged by size: 3170 1071 999 184 85 24 BsmI GAATG_Cn' Cuts at: 3044 3137 3044 Size: 93 5440 BsmBI CGTCTCn'nnnn_ Cuts at: 2391 2391 Size: 5533 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2387 2571 2595 2852 3594 3679 4750 Size: 399 597 184 24 257 742 85 1071 Cuts at: 4750 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 597 399 257 184 85 24 BspEI T'CCGG_A Cuts at: 5196 5196 Size: 5533 BspHI T'CATG_A Cuts at: 3422 5152 3422 Size: 1730 3803 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2241 2888 844 Size: 1397 647 3489 Fragments arranged by size: 3489 1397 647 BsrBI CCG'CTC Cuts at: 1394 1823 3420 1394 Size: 429 1597 3507 Fragments arranged by size: 3507 1597 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4028 4202 65 Size: 1183 2780 174 1396 Fragments arranged by size: 2780 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4177 4364 1700 Size: 110 2367 187 2869 Fragments arranged by size: 2869 2367 187 110 BsrGI T'GTAC_A Cuts at: 96 2349 96 Size: 2253 3280 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5533 BssSI C'ACGA_G Cuts at: 3593 4605 3593 Size: 1012 4521 BstAPI GCAn_nnn'nTGC Cuts at: 794 2662 794 Size: 1868 3665 BstBI TT'CG_AA Cuts at: 4337 4337 Size: 5533 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5533 BstXI CCAn_nnnn'nTGG Cuts at: 2302 2584 2302 Size: 282 5251 BstYI r'GATC_y Cuts at: 1972 2139 2863 3230 3615 3632 5084 5170 5182 Size: 167 724 367 385 17 1452 86 12 Cuts at: 5182 5528 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 724 385 367 346 167 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4981 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2557 487 Size: 683 56 1331 3463 Fragments arranged by size: 3463 1331 683 56 BtsI GCAGTG_nn' Cuts at: 2822 3049 3843 3863 2822 Size: 227 794 20 4492 Fragments arranged by size: 4492 794 227 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5533 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5498 DraI TTT'AAA Cuts at: 2837 2837 Size: 5533 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4540 809 Size: 507 3224 1802 Fragments arranged by size: 3224 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2625 2746 2980 Size: 54 1425 50 275 18 795 121 234 Cuts at: 2980 3869 5498 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 795 275 234 121 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2980 1537 Size: 275 1168 4090 Fragments arranged by size: 4090 1168 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2528 2598 3463 1353 Size: 1175 70 865 3423 Fragments arranged by size: 3423 1175 865 70 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4120 4494 4640 387 Size: 1064 138 2531 374 146 1280 Fragments arranged by size: 2531 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2342 2504 2515 2549 3596 4193 4980 Size: 393 710 162 11 34 1047 597 787 Cuts at: 4980 5248 1239 Size: 268 1524 Fragments arranged by size: 1524 1047 787 710 597 393 268 162 34 11 EcoNI CCTnn'n_nnAGG Cuts at: 2324 2324 Size: 5533 EcoO109I rG'GnC_Cy Cuts at: 2785 2785 Size: 5533 EcoRI G'AATT_C Cuts at: 2087 2842 3220 2087 Size: 755 378 4400 Fragments arranged by size: 4400 755 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2524 2539 2524 Size: 15 5518 FspI TGC'GCA Cuts at: 4039 4039 Size: 5533 HaeII r_GCGC'y Cuts at: 1678 2555 4680 1678 Size: 877 2125 2531 Fragments arranged by size: 2531 2125 877 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2297 2329 4174 4206 4248 Size: 32 135 32 725 32 1845 32 42 Cuts at: 4248 4280 1373 Size: 32 2626 Fragments arranged by size: 2626 1845 725 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2877 3123 669 Size: 988 281 939 246 3079 Fragments arranged by size: 3079 988 939 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5533 HpaI GTT'AAC Cuts at: 3123 3123 Size: 5533 KpnI G_GTAC'C Cuts at: 2858 2858 Size: 5533 MfeI C'AATT_G Cuts at: 3132 3132 Size: 5533 MluI A'CGCG_T Cuts at: 2052 5409 2052 Size: 3357 2176 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4647 4831 1305 Size: 3342 184 2007 Fragments arranged by size: 3342 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3711 Fragments arranged by size: 3711 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2300 3491 3850 4009 518 Size: 648 1134 1191 359 159 2042 Fragments arranged by size: 2042 1191 1134 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2245 2614 2949 3626 4774 5019 1606 Size: 639 369 335 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 639 369 335 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4981 NdeI CA'TA_TG Cuts at: 387 387 Size: 5533 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5533 NotI GC'GGCC_GC Cuts at: 2980 2980 Size: 5533 NspI r_CATG'y Cuts at: 1132 1229 2891 4436 1132 Size: 97 1662 1545 2229 Fragments arranged by size: 2229 1662 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5533 PciI A'CATG_T Cuts at: 4432 4432 Size: 5533 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5533 PfoI T'CCnGG_A Cuts at: 2526 2583 2526 Size: 57 5476 PmeI GTTT'AAAC Cuts at: 2837 2837 Size: 5533 PmlI CAC'GTG Cuts at: 2204 2752 2204 Size: 548 4985 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3602 3634 5115 5147 3602 Size: 32 1481 32 3988 Fragments arranged by size: 3988 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2785 2785 Size: 5533 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5533 PsiI TTA'TAA Cuts at: 3103 3103 Size: 5533 PstI C_TGCA'G Cuts at: 830 2238 2829 2885 830 Size: 1408 591 56 3478 Fragments arranged by size: 3478 1408 591 56 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5533 PvuII CAG'CTG Cuts at: 2245 2614 2245 Size: 369 5164 SalI G'TCGA_C Cuts at: 1936 2875 1936 Size: 939 4594 SapI GCTCTTCn'nnn_ Cuts at: 2528 2528 Size: 5533 SbfI CC_TGCA'GG Cuts at: 2885 2885 Size: 5533 ScaI AGT'ACT Cuts at: 3781 5493 3781 Size: 1712 3821 SfcI C'TryA_G Cuts at: 826 1045 2092 2234 2825 2881 4016 4697 4888 Size: 219 1047 142 591 56 1135 681 191 Cuts at: 4888 826 Size: 1471 Fragments arranged by size: 1471 1135 1047 681 591 219 191 142 56 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5533 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5533 SmaI CCC'GGG Cuts at: 1872 2860 1872 Size: 988 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3637 4538 4800 820 Size: 197 926 1694 901 262 1553 Fragments arranged by size: 1694 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3324 493 Size: 2831 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5533 SphI G_CATG'C Cuts at: 1132 2891 1132 Size: 1759 3774 SspI AAT'ATT Cuts at: 5 52 3457 5 Size: 47 3405 2081 Fragments arranged by size: 3405 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5533 StyI C'CwwG_G Cuts at: 513 1065 2559 2972 513 Size: 552 1494 413 3074 Fragments arranged by size: 3074 1494 552 413 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2297 3560 3577 3730 3915 4360 325 Size: 1255 717 1263 17 153 185 445 1498 Fragments arranged by size: 1498 1263 1255 717 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2349 3779 5491 Size: 274 80 33 51 1443 372 1430 1712 Cuts at: 5491 96 Size: 138 Fragments arranged by size: 1712 1443 1430 372 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5493 5525 5493 Size: 32 5501 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5533 XbaI T'CTAG_A Cuts at: 2869 2869 Size: 5533 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2351 2351 Size: 5533 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5533 XmaI C'CCGG_G Cuts at: 1870 2858 1870 Size: 988 4545 XmnI GAAnn'nnTTC Cuts at: 3662 3662 Size: 5533 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2401 3341 276 Size: 53 83 186 936 867 940 2468 Fragments arranged by size: 2468 940 936 867 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI