(Circular) (Six-base) MAPSORT of: pFN21AB7349.seq Check: 9559 from: 1 to: 7753 pFN21AB7349 7753 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2317 2844 4576 4588 2317 Size: 527 1732 12 5482 Fragments arranged by size: 5482 1732 527 12 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2025 5563 278 Size: 53 83 186 936 489 3538 2468 Fragments arranged by size: 3538 2468 936 489 186 83 53 AccI GT'mk_AC Cuts at: 1937 5096 1937 Size: 3159 4594 Acc65I G'GTAC_C Cuts at: 5074 5074 Size: 7753 AclI AA'CG_TT Cuts at: 1563 5880 6253 1563 Size: 4317 373 3063 Fragments arranged by size: 4317 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2462 2561 2648 2660 3601 3688 3741 3896 Size: 830 99 87 12 941 87 53 155 Cuts at: 3896 4274 4289 4406 4679 5816 7200 7468 1632 Size: 378 15 117 273 1137 1384 268 1917 Fragments arranged by size: 1917 1384 1137 941 830 378 273 268 155 117 99 87 87 53 15 12 AfeI AGC'GCT Cuts at: 3098 4055 4489 3098 Size: 957 434 6362 Fragments arranged by size: 6362 957 434 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7556 AflIII A'CryG_T Cuts at: 1203 2040 6652 7629 1203 Size: 837 4612 977 1327 Fragments arranged by size: 4612 1327 977 837 AgeI A'CCGG_T Cuts at: 6584 6584 Size: 7753 AhdI GACnn_n'nnGTC Cuts at: 6482 6482 Size: 7753 AleI CACnn'nnGTG Cuts at: 1166 4965 1166 Size: 3799 3954 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4091 4332 4656 4877 1184 Size: 2907 241 324 221 4060 Fragments arranged by size: 4060 2907 324 241 221 AlwNI CAG_nnn'CTG Cuts at: 2059 2071 2332 3460 3928 5005 5496 7068 2059 Size: 12 261 1128 468 1077 491 1572 2744 Fragments arranged by size: 2744 1572 1128 1077 491 468 261 12 ApaI G_GGCC'C Cuts at: 3401 4422 3401 Size: 1021 6732 ApaLI G'TGCA_C Cuts at: 2633 5810 6966 2633 Size: 3177 1156 3420 Fragments arranged by size: 3420 3177 1156 ApoI r'AATT_y Cuts at: 1493 3956 5062 5288 5440 7549 1493 Size: 2463 1106 226 152 2109 1697 Fragments arranged by size: 2463 2109 1697 1106 226 152 AseI AT'TA_AT Cuts at: 160 1032 6307 160 Size: 872 5275 1606 Fragments arranged by size: 5275 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7753 AvaI C'yCGr_G Cuts at: 1870 1943 2240 2888 3494 4100 4848 5078 1870 Size: 73 297 648 606 606 748 230 4545 Fragments arranged by size: 4545 748 648 606 606 297 230 73 BamHI G'GATC_C Cuts at: 3645 4906 5083 5450 3645 Size: 1261 177 367 5948 Fragments arranged by size: 5948 1261 367 177 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4967 5074 6529 618 Size: 325 217 627 3180 107 1455 1842 Fragments arranged by size: 3180 1842 1455 627 325 217 107 BanII G_rGCy'C Cuts at: 1391 2241 2889 3039 3401 3551 3561 3642 4359 Size: 850 648 150 362 150 10 81 717 Cuts at: 4359 4422 4895 4941 5072 1391 Size: 63 473 46 131 4072 Fragments arranged by size: 4072 850 717 648 473 362 150 150 131 81 63 46 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2417 3729 4394 928 Size: 971 518 1312 665 4287 Fragments arranged by size: 4287 1312 971 665 518 BbvCI CC'TCA_GC Cuts at: 3774 3774 Size: 7753 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3191 3225 5944 5978 1903 Size: 34 50 34 1170 34 2719 34 3678 Fragments arranged by size: 3678 2719 1170 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5645 6855 5645 Size: 1210 6543 BclI T'GATC_A Cuts at: 1548 1548 Size: 7753 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3236 3795 6364 Size: 107 122 71 631 605 1564 559 2569 Cuts at: 6364 136 Size: 1525 Fragments arranged by size: 2569 1564 1525 631 605 559 122 107 71 BglII A'GATC_T Cuts at: 7748 7748 Size: 7753 BlpI GC'TnA_GC Cuts at: 2322 2669 2984 3038 4158 5170 2322 Size: 347 315 54 1120 1012 4905 Fragments arranged by size: 4905 1120 1012 347 315 54 Bme1580I G_kGCm'C Cuts at: 1790 2637 3401 4422 4972 5814 6970 1790 Size: 847 764 1021 550 842 1156 2573 Fragments arranged by size: 2573 1156 1021 847 842 764 550 BmgBI CAC'GTC Cuts at: 1318 2041 2953 1318 Size: 723 912 6118 Fragments arranged by size: 6118 912 723 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3163 5484 6442 Size: 416 53 83 399 24 1746 2321 958 Cuts at: 6442 442 Size: 1753 Fragments arranged by size: 2321 1753 1746 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7753 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3544 3829 3544 Size: 285 7468 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2030 2207 2291 2366 2711 3077 3158 3920 Size: 791 177 84 75 345 366 81 762 Cuts at: 3920 3968 3982 4555 4694 6413 1239 Size: 48 14 573 139 1719 2579 Fragments arranged by size: 2579 1719 791 762 573 366 345 177 139 84 81 75 48 14 Bpu10I CC'TnA_GC Cuts at: 3440 3774 4037 4622 3440 Size: 334 263 585 6571 Fragments arranged by size: 6571 585 334 263 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4034 4343 5878 6743 7041 4034 Size: 309 1535 865 298 4746 Fragments arranged by size: 4746 1535 865 309 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2209 2612 6416 882 Size: 617 710 403 3804 2219 Fragments arranged by size: 3804 2219 710 617 403 BsaAI yAC'GTr Cuts at: 493 1204 5544 493 Size: 711 4340 2702 Fragments arranged by size: 4340 2702 711 BsaBI GATnn'nnATC Cuts at: 7747 7747 Size: 7753 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2022 3147 5560 5942 Size: 53 83 186 936 489 1125 2413 382 Cuts at: 5942 6577 275 Size: 635 1451 Fragments arranged by size: 2413 1451 1125 936 635 489 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6186 6584 6858 7005 7416 6186 Size: 398 274 147 411 6523 Fragments arranged by size: 6523 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2027 2057 3095 3125 1178 Size: 30 165 30 624 30 1038 30 5806 Fragments arranged by size: 5806 1038 624 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 2288 2291 2483 2546 2603 2666 2866 Size: 862 240 3 192 63 57 63 200 Cuts at: 2866 2867 3017 3071 3854 4160 4469 5006 1186 Size: 1 150 54 783 306 309 537 3933 Fragments arranged by size: 3933 862 783 537 309 306 240 200 192 150 63 63 57 54 3 1 BseYI C'CCAG_C Cuts at: 2788 3692 3996 4079 5034 6956 7452 2788 Size: 904 304 83 955 1922 496 3089 Fragments arranged by size: 3089 1922 955 904 496 304 83 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2441 2454 4007 4165 1163 Size: 573 705 13 1553 158 4751 Fragments arranged by size: 4751 1553 705 573 158 13 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2354 3015 5203 5964 6992 1540 Size: 275 186 353 661 2188 761 1028 2301 Fragments arranged by size: 2301 2188 1028 761 661 353 275 186 BsiHKAI G_wGCw'C Cuts at: 2241 2637 2889 2943 2985 3039 3081 3503 4359 Size: 396 252 54 42 54 42 422 856 Cuts at: 4359 4895 5814 5899 6970 2241 Size: 536 919 85 1071 3024 Fragments arranged by size: 3024 1071 919 856 536 422 396 252 85 54 54 42 42 BsmI GAATG_Cn' Cuts at: 2362 3469 4162 5264 5357 2362 Size: 1107 693 1102 93 4758 Fragments arranged by size: 4758 1107 1102 693 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3134 3134 Size: 7753 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2241 2637 2889 2943 2985 3039 3081 Size: 399 451 396 252 54 42 54 42 Cuts at: 3081 3401 3503 3551 3561 3642 4359 4422 4895 Size: 320 102 48 10 81 717 63 473 Cuts at: 4895 4941 4972 5072 5814 5899 6970 1391 Size: 46 31 100 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 717 473 451 399 396 320 252 102 100 85 81 63 54 54 48 46 42 42 31 10 BspEI T'CCGG_A Cuts at: 7416 7416 Size: 7753 BspHI T'CATG_A Cuts at: 4029 5642 7372 4029 Size: 1613 1730 4410 Fragments arranged by size: 4410 1730 1613 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2099 2317 2673 2844 3633 4576 4588 5108 Size: 1255 218 356 171 789 943 12 520 Cuts at: 5108 844 Size: 3489 Fragments arranged by size: 3489 1255 943 789 520 356 218 171 12 BsrBI CCG'CTC Cuts at: 1394 1823 2388 2550 2934 3823 4348 4411 4564 Size: 429 565 162 384 889 525 63 153 Cuts at: 4564 5640 1394 Size: 1076 3507 Fragments arranged by size: 3507 1076 889 565 525 429 384 162 153 63 BsrDI GCAATG_nn' Cuts at: 65 1248 6248 6422 65 Size: 1183 5000 174 1396 Fragments arranged by size: 5000 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2109 2153 6397 6584 1700 Size: 110 299 44 4244 187 2869 Fragments arranged by size: 4244 2869 299 187 110 44 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7753 BssHII G'CGCG_C Cuts at: 1925 2574 1925 Size: 649 7104 BssSI C'ACGA_G Cuts at: 2942 3200 5813 6825 2942 Size: 258 2613 1012 3870 Fragments arranged by size: 3870 2613 1012 258 BstAPI GCAn_nnn'nTGC Cuts at: 794 2059 2091 2332 3766 794 Size: 1265 32 241 1434 4781 Fragments arranged by size: 4781 1434 1265 241 32 BstBI TT'CG_AA Cuts at: 6557 6557 Size: 7753 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7753 BstYI r'GATC_y Cuts at: 1972 3645 4716 4906 5083 5450 5835 5852 7304 Size: 1673 1071 190 177 367 385 17 1452 Cuts at: 7304 7390 7402 7748 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1673 1452 1071 385 367 346 190 177 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2763 3902 2763 Size: 1139 6614 BtgI C'CryG_G Cuts at: 513 1065 2179 4288 513 Size: 552 1114 2109 3978 Fragments arranged by size: 3978 2109 1114 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7014 Fragments arranged by size: 7014 683 56 BtsI GCAGTG_nn' Cuts at: 3533 4714 5269 6063 6083 3533 Size: 1181 555 794 20 5203 Fragments arranged by size: 5203 1181 794 555 20 ClaI AT'CG_AT Cuts at: 1554 3291 1554 Size: 1737 6016 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7718 DraI TTT'AAA Cuts at: 4767 5057 4767 Size: 290 7463 DraIII CAC_nnn'GTG Cuts at: 4709 4709 Size: 7753 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3202 4652 6760 809 Size: 507 1886 1450 2108 1802 Fragments arranged by size: 2108 1886 1802 1450 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2155 2595 2922 Size: 54 1425 50 275 18 325 440 327 Cuts at: 2922 3012 4233 5200 6089 7718 8 Size: 90 1221 967 889 1629 43 Fragments arranged by size: 1629 1425 1221 967 889 440 327 325 275 90 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3012 5200 1537 Size: 275 1200 2188 4090 Fragments arranged by size: 4090 2188 1200 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2280 2844 4031 4137 5683 1353 Size: 927 564 1187 106 1546 3423 Fragments arranged by size: 3423 1546 1187 927 564 106 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2811 4854 6340 6714 6860 387 Size: 1064 138 1222 2043 1486 374 146 1280 Fragments arranged by size: 2043 1486 1280 1222 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2239 2887 3037 4357 4893 2239 Size: 648 150 1320 536 5099 Fragments arranged by size: 5099 1320 648 536 150 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 2207 2291 2366 2462 2561 2648 Size: 393 398 177 84 75 96 99 87 Cuts at: 2648 2660 2711 3077 3158 3601 3688 3741 3896 Size: 12 51 366 81 443 87 53 155 Cuts at: 3896 3920 3968 3982 4274 4289 4406 4555 4679 Size: 24 48 14 292 15 117 149 124 Cuts at: 4679 4694 5816 6413 7200 7468 1239 Size: 15 1122 597 787 268 1524 Fragments arranged by size: 1524 1122 787 597 443 398 393 366 292 268 177 155 149 124 117 99 96 87 87 84 81 75 53 51 48 24 15 15 14 12 EcoNI CCTnn'n_nnAGG Cuts at: 2396 2396 Size: 7753 EcoO109I rG'GnC_Cy Cuts at: 2563 2759 3379 3397 3398 3622 4418 4654 4984 Size: 196 620 18 1 224 796 236 330 Cuts at: 4984 2563 Size: 5332 Fragments arranged by size: 5332 796 620 330 236 224 196 18 1 EcoRI G'AATT_C Cuts at: 5062 5440 5062 Size: 378 7375 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4030 4766 5026 4030 Size: 736 260 6757 Fragments arranged by size: 6757 736 260 FspI TGC'GCA Cuts at: 2117 6259 2117 Size: 4142 3611 HaeII r_GCGC'y Cuts at: 1678 2131 2875 2960 3100 3899 4057 4491 6900 Size: 453 744 85 140 799 158 434 2409 Cuts at: 6900 1678 Size: 2531 Fragments arranged by size: 2531 2409 799 744 453 434 158 140 85 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3095 3127 3578 3610 3662 Size: 32 135 32 1523 32 451 32 52 Cuts at: 3662 3694 4141 4173 6394 6426 6468 6500 1373 Size: 32 447 32 2221 32 42 32 2626 Fragments arranged by size: 2626 2221 1523 451 447 135 52 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5097 5343 669 Size: 988 281 3159 246 3079 Fragments arranged by size: 3159 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2501 748 Size: 1753 6000 HpaI GTT'AAC Cuts at: 5343 5343 Size: 7753 KpnI G_GTAC'C Cuts at: 5078 5078 Size: 7753 MfeI C'AATT_G Cuts at: 5352 5352 Size: 7753 MluI A'CGCG_T Cuts at: 7629 7629 Size: 7753 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2943 3111 3241 6867 7051 1305 Size: 1638 168 130 3626 184 2007 Fragments arranged by size: 3626 2007 1638 184 168 130 MscI TGG'CCA Cuts at: 10 64 1489 1832 2924 10 Size: 54 1425 343 1092 4839 Fragments arranged by size: 4839 1425 1092 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2219 2745 3431 3983 4244 4965 5711 Size: 648 1053 526 686 552 261 721 746 Cuts at: 5711 6070 6229 518 Size: 359 159 2042 Fragments arranged by size: 2042 1053 746 721 686 648 552 526 359 261 159 MspA1I CmG'CkG Cuts at: 1606 2050 2071 2299 3042 3763 3874 3940 4194 Size: 444 21 228 743 721 111 66 254 Cuts at: 4194 4444 4513 4555 4630 4692 5169 5846 6994 Size: 250 69 42 75 62 477 677 1148 Cuts at: 6994 7239 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 743 721 677 477 444 254 250 245 228 111 75 69 66 62 42 21 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7201 NdeI CA'TA_TG Cuts at: 387 387 Size: 7753 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7753 NotI GC'GGCC_GC Cuts at: 3012 5200 3012 Size: 2188 5565 NspI r_CATG'y Cuts at: 1132 1229 3982 5111 6656 1132 Size: 97 2753 1129 1545 2229 Fragments arranged by size: 2753 2229 1545 1129 97 PasI CC'CwG_GG Cuts at: 1348 2392 3187 3402 3553 4650 4991 1348 Size: 1044 795 215 151 1097 341 4110 Fragments arranged by size: 4110 1097 1044 795 341 215 151 PciI A'CATG_T Cuts at: 6652 6652 Size: 7753 PflMI CCAn_nnn'nTGG Cuts at: 1108 3865 1108 Size: 2757 4996 PfoI T'CCnGG_A Cuts at: 4275 4275 Size: 7753 PmeI GTTT'AAAC Cuts at: 5057 5057 Size: 7753 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5822 5854 7335 7367 5822 Size: 32 1481 32 6208 Fragments arranged by size: 6208 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2563 2759 3622 4654 4984 2563 Size: 196 863 1032 330 5332 Fragments arranged by size: 5332 1032 863 330 196 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7753 PsiI TTA'TAA Cuts at: 5323 5323 Size: 7753 PspOMI G'GGCC_C Cuts at: 3397 4418 3397 Size: 1021 6732 PspXI vC'TCGA_Gb Cuts at: 2240 2888 4100 2240 Size: 648 1212 5893 Fragments arranged by size: 5893 1212 648 PstI C_TGCA'G Cuts at: 830 2055 2106 2337 2841 3027 3063 3227 3453 Size: 1225 51 231 504 186 36 164 226 Cuts at: 3453 3762 3924 4518 4554 4572 4614 4635 5105 Size: 309 162 594 36 18 42 21 470 Cuts at: 5105 830 Size: 3478 Fragments arranged by size: 3478 1225 594 504 470 309 231 226 186 164 162 51 42 36 36 21 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7753 PvuII CAG'CTG Cuts at: 2050 2071 3763 3874 4194 4513 4555 4630 4692 Size: 21 1692 111 320 319 42 75 62 Cuts at: 4692 2050 Size: 5111 Fragments arranged by size: 5111 1692 320 319 111 75 62 42 21 RsrII CG'GwC_CG Cuts at: 3214 3214 Size: 7753 SacI G_AGCT'C Cuts at: 2241 2889 3039 4359 4895 2241 Size: 648 150 1320 536 5099 Fragments arranged by size: 5099 1320 648 536 150 SalI G'TCGA_C Cuts at: 1936 5095 1936 Size: 3159 4594 SapI GCTCTTCn'nnn_ Cuts at: 2280 4031 2280 Size: 1751 6002 SbfI CC_TGCA'GG Cuts at: 5105 5105 Size: 7753 ScaI AGT'ACT Cuts at: 6001 7713 6001 Size: 1712 6041 SfcI C'TryA_G Cuts at: 826 1045 2051 2102 2333 2837 3023 3059 3223 Size: 219 1006 51 231 504 186 36 164 Cuts at: 3223 3449 3758 3920 4439 4483 4514 4550 4568 Size: 226 309 162 519 44 31 36 18 Cuts at: 4568 4610 4631 5101 6236 6917 7108 826 Size: 42 21 470 1135 681 191 1471 Fragments arranged by size: 1471 1135 1006 681 519 504 470 309 231 226 219 191 186 164 162 51 44 42 36 36 31 21 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7753 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7753 SmaI CCC'GGG Cuts at: 1872 4850 5080 1872 Size: 2978 230 4545 Fragments arranged by size: 4545 2978 230 SmlI C'TyrA_G Cuts at: 820 1017 1943 2240 2888 4049 4100 4358 5857 Size: 197 926 297 648 1161 51 258 1499 Cuts at: 5857 6758 7020 820 Size: 901 262 1553 Fragments arranged by size: 1553 1499 1161 926 901 648 297 262 258 197 51 SnaBI TAC'GTA Cuts at: 493 5544 493 Size: 5051 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7753 SphI G_CATG'C Cuts at: 1132 5111 1132 Size: 3979 3774 SspI AAT'ATT Cuts at: 5 52 5677 5 Size: 47 5625 2081 Fragments arranged by size: 5625 2081 47 StuI AGG'CCT Cuts at: 1517 2709 3709 4178 1517 Size: 1192 1000 469 5092 Fragments arranged by size: 5092 1192 1000 469 StyI C'CwwG_G Cuts at: 513 1065 2508 3543 3589 4320 4326 4923 5192 Size: 552 1443 1035 46 731 6 597 269 Cuts at: 5192 513 Size: 3074 Fragments arranged by size: 3074 1443 1035 731 597 552 269 46 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2368 2636 2780 4955 5780 5797 5950 Size: 1255 788 268 144 2175 825 17 153 Cuts at: 5950 6135 6580 325 Size: 185 445 1498 Fragments arranged by size: 2175 1498 1255 825 788 445 268 185 153 144 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4125 4218 5999 Size: 274 80 33 51 1443 2148 93 1781 Cuts at: 5999 7711 96 Size: 1712 138 Fragments arranged by size: 2148 1781 1712 1443 274 138 93 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3338 3370 7713 7745 3338 Size: 32 4343 32 3346 Fragments arranged by size: 4343 3346 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7753 XbaI T'CTAG_A Cuts at: 5089 5089 Size: 7753 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3295 3295 Size: 7753 XhoI C'TCGA_G Cuts at: 1943 2240 2888 4100 1943 Size: 297 648 1212 5596 Fragments arranged by size: 5596 1212 648 297 XmaI C'CCGG_G Cuts at: 1870 4848 5078 1870 Size: 2978 230 4545 Fragments arranged by size: 4545 2978 230 XmnI GAAnn'nnTTC Cuts at: 2289 3467 5882 2289 Size: 1178 2415 4160 Fragments arranged by size: 4160 2415 1178 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2023 5561 276 Size: 53 83 186 936 489 3538 2468 Fragments arranged by size: 3538 2468 936 489 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI SacII SanDI SexAI SfiI SfoI SrfI SwaI