(Circular) (Six-base) MAPSORT of: pFN21AE0270.seq Check: 4808 from: 1 to: 5437 pFN21AE0270 5437 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3247 278 Size: 53 83 186 936 1711 2468 Fragments arranged by size: 2468 1711 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2780 1937 Size: 843 4594 Acc65I G'GTAC_C Cuts at: 2758 2758 Size: 5437 AclI AA'CG_TT Cuts at: 1563 3564 3937 1563 Size: 2001 373 3063 Fragments arranged by size: 3063 2001 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2421 3500 4884 5152 1632 Size: 789 1079 1384 268 1917 Fragments arranged by size: 1917 1384 1079 789 268 AfeI AGC'GCT Cuts at: 2186 2186 Size: 5437 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5240 AflIII A'CryG_T Cuts at: 1203 2722 4336 5313 1203 Size: 1519 1614 977 1327 Fragments arranged by size: 1614 1519 1327 977 AgeI A'CCGG_T Cuts at: 4268 4268 Size: 5437 AhdI GACnn_n'nnGTC Cuts at: 2723 4166 2723 Size: 1443 3994 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5437 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5437 AlwNI CAG_nnn'CTG Cuts at: 2687 3180 4752 2687 Size: 493 1572 3372 Fragments arranged by size: 3372 1572 493 ApaLI G'TGCA_C Cuts at: 3494 4650 3494 Size: 1156 4281 ApoI r'AATT_y Cuts at: 1493 2746 2972 3124 5233 1493 Size: 1253 226 152 2109 1697 Fragments arranged by size: 2109 1697 1253 226 152 AseI AT'TA_AT Cuts at: 160 1032 3991 160 Size: 872 2959 1606 Fragments arranged by size: 2959 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5437 AvaI C'yCGr_G Cuts at: 1870 1943 2762 1870 Size: 73 819 4545 Fragments arranged by size: 4545 819 73 BamHI G'GATC_C Cuts at: 2008 2767 3134 2008 Size: 759 367 4311 Fragments arranged by size: 4311 759 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2015 2758 4213 618 Size: 325 217 627 228 743 1455 1842 Fragments arranged by size: 1842 1455 743 627 325 228 217 BanII G_rGCy'C Cuts at: 1391 2066 2756 1391 Size: 675 690 4072 Fragments arranged by size: 4072 690 675 BbeI G_GCGC'C Cuts at: 2019 2019 Size: 5437 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4466 BbvCI CC'TCA_GC Cuts at: 2329 2329 Size: 5437 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3628 3662 1903 Size: 34 1691 34 3678 Fragments arranged by size: 3678 1691 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2675 3329 4539 2675 Size: 654 1210 3573 Fragments arranged by size: 3573 1210 654 BclI T'GATC_A Cuts at: 1548 1548 Size: 5437 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2113 4048 136 Size: 107 122 71 631 605 441 1935 1525 Fragments arranged by size: 1935 1525 631 605 441 122 107 71 BglII A'GATC_T Cuts at: 2286 5432 2286 Size: 3146 2291 BlpI GC'TnA_GC Cuts at: 2854 2854 Size: 5437 Bme1580I G_kGCm'C Cuts at: 1790 3498 4654 1790 Size: 1708 1156 2573 Fragments arranged by size: 2573 1708 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5437 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2547 3168 4126 Size: 416 53 83 399 24 1130 621 958 Cuts at: 4126 442 Size: 1753 Fragments arranged by size: 1753 1130 958 621 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5437 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4097 1239 Size: 2858 2579 Bpu10I CC'TnA_GC Cuts at: 2329 2329 Size: 5437 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3562 4427 4725 3562 Size: 865 298 4274 Fragments arranged by size: 4274 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4100 882 Size: 617 2601 2219 Fragments arranged by size: 2601 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3228 493 Size: 711 2024 2702 Fragments arranged by size: 2702 2024 711 BsaBI GATnn'nnATC Cuts at: 5431 5431 Size: 5437 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2016 3244 3626 4261 Size: 53 83 186 936 483 1228 382 635 Cuts at: 4261 275 Size: 1451 Fragments arranged by size: 1451 1228 936 635 483 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3870 4268 4542 4689 5100 3870 Size: 398 274 147 411 4207 Fragments arranged by size: 4207 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5212 Fragments arranged by size: 5212 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5437 BseYI C'CCAG_C Cuts at: 4640 5136 4640 Size: 496 4941 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4864 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2121 2887 3648 4676 1540 Size: 275 186 120 766 761 1028 2301 Fragments arranged by size: 2301 1028 766 761 275 186 120 BsiHKAI G_wGCw'C Cuts at: 3498 3583 4654 3498 Size: 85 1071 4281 Fragments arranged by size: 4281 1071 85 BsmI GAATG_Cn' Cuts at: 2948 3041 2948 Size: 93 5344 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2756 3498 3583 4654 1391 Size: 399 276 690 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 690 399 276 85 BspEI T'CCGG_A Cuts at: 5100 5100 Size: 5437 BspHI T'CATG_A Cuts at: 3326 5056 3326 Size: 1730 3707 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2125 2291 2792 844 Size: 1281 166 501 3489 Fragments arranged by size: 3489 1281 501 166 BsrBI CCG'CTC Cuts at: 1394 1823 3324 1394 Size: 429 1501 3507 Fragments arranged by size: 3507 1501 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3932 4106 65 Size: 1183 2684 174 1396 Fragments arranged by size: 2684 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2116 4081 4268 1700 Size: 110 306 1965 187 2869 Fragments arranged by size: 2869 1965 306 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5437 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5437 BssSI C'ACGA_G Cuts at: 3497 4509 3497 Size: 1012 4425 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5437 BstBI TT'CG_AA Cuts at: 4241 4241 Size: 5437 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5437 BstXI CCAn_nnnn'nTGG Cuts at: 2179 2179 Size: 5437 BstYI r'GATC_y Cuts at: 1972 2008 2286 2767 3134 3519 3536 4988 5074 Size: 36 278 481 367 385 17 1452 86 Cuts at: 5074 5086 5432 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 481 385 367 346 278 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2155 2226 513 Size: 552 939 151 71 3724 Fragments arranged by size: 3724 939 552 151 71 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4698 Fragments arranged by size: 4698 683 56 BtsI GCAGTG_nn' Cuts at: 2953 3747 3767 2953 Size: 794 20 4623 Fragments arranged by size: 4623 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5437 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5402 DraI TTT'AAA Cuts at: 2741 2741 Size: 5437 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4444 809 Size: 507 3128 1802 Fragments arranged by size: 3128 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2083 2118 2884 Size: 54 1425 50 275 18 253 35 766 Cuts at: 2884 3773 5402 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 766 275 253 54 50 43 35 18 EagI C'GGCC_G Cuts at: 1537 1812 2118 2884 1537 Size: 275 306 766 4090 Fragments arranged by size: 4090 766 306 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3367 1353 Size: 2014 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4024 4398 4544 387 Size: 1064 138 2435 374 146 1280 Fragments arranged by size: 2435 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2421 3500 4097 4884 5152 1239 Size: 393 789 1079 597 787 268 1524 Fragments arranged by size: 1524 1079 789 787 597 393 268 EcoRI G'AATT_C Cuts at: 2746 3124 2746 Size: 378 5059 EcoRV GAT'ATC Cuts at: 2374 2374 Size: 5437 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2590 2590 Size: 5437 FseI GG_CCGG'CC Cuts at: 2120 2120 Size: 5437 FspI TGC'GCA Cuts at: 3943 3943 Size: 5437 HaeII r_GCGC'y Cuts at: 1678 2019 2075 2188 4584 1678 Size: 341 56 113 2396 2531 Fragments arranged by size: 2531 2396 341 113 56 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2271 2303 2709 2741 4078 Size: 32 135 32 699 32 406 32 1337 Cuts at: 4078 4110 4152 4184 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1337 699 406 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2236 2781 3027 669 Size: 988 281 298 545 246 3079 Fragments arranged by size: 3079 988 545 298 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5437 HpaI GTT'AAC Cuts at: 3027 3027 Size: 5437 KasI G'GCGC_C Cuts at: 2015 2015 Size: 5437 KpnI G_GTAC'C Cuts at: 2762 2762 Size: 5437 MfeI C'AATT_G Cuts at: 3036 3036 Size: 5437 MluI A'CGCG_T Cuts at: 5313 5313 Size: 5437 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2443 2454 4551 4735 1305 Size: 1138 11 2097 184 2007 Fragments arranged by size: 2097 2007 1138 184 11 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3615 Fragments arranged by size: 3615 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2540 3395 3754 3913 518 Size: 648 1374 855 359 159 2042 Fragments arranged by size: 2042 1374 855 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2157 2853 3530 4678 4923 1606 Size: 551 696 677 1148 245 2120 Fragments arranged by size: 2120 1148 696 677 551 245 NaeI GCC'GGC Cuts at: 2118 2118 Size: 5437 NarI GG'CG_CC Cuts at: 2016 2016 Size: 5437 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3946 Fragments arranged by size: 3946 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5437 NgoMIV G'CCGG_C Cuts at: 2116 2116 Size: 5437 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5437 NotI GC'GGCC_GC Cuts at: 2884 2884 Size: 5437 NspI r_CATG'y Cuts at: 1132 1229 2726 2795 4340 1132 Size: 97 1497 69 1545 2229 Fragments arranged by size: 2229 1545 1497 97 69 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5437 PciI A'CATG_T Cuts at: 2722 4336 2722 Size: 1614 3823 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5437 PmeI GTTT'AAAC Cuts at: 2741 2741 Size: 5437 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3506 3538 5019 5051 3506 Size: 32 1481 32 3892 Fragments arranged by size: 3892 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5437 PsiI TTA'TAA Cuts at: 3007 3007 Size: 5437 PstI C_TGCA'G Cuts at: 830 2028 2789 830 Size: 1198 761 3478 Fragments arranged by size: 3478 1198 761 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5437 SacII CC_GC'GG Cuts at: 2158 2158 Size: 5437 SalI G'TCGA_C Cuts at: 1936 2779 1936 Size: 843 4594 SbfI CC_TGCA'GG Cuts at: 2789 2789 Size: 5437 ScaI AGT'ACT Cuts at: 3685 5397 3685 Size: 1712 3725 SfcI C'TryA_G Cuts at: 826 1045 2024 2253 2785 3920 4601 4792 826 Size: 219 979 229 532 1135 681 191 1471 Fragments arranged by size: 1471 1135 979 681 532 229 219 191 SfoI GGC'GCC Cuts at: 2017 2017 Size: 5437 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5437 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5437 SmaI CCC'GGG Cuts at: 1872 2764 1872 Size: 892 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3541 4442 4704 820 Size: 197 926 1598 901 262 1553 Fragments arranged by size: 1598 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3228 493 Size: 2735 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5437 SphI G_CATG'C Cuts at: 1132 2795 1132 Size: 1663 3774 SspI AAT'ATT Cuts at: 5 52 3361 5 Size: 47 3309 2081 Fragments arranged by size: 3309 2081 47 StuI AGG'CCT Cuts at: 1517 2213 1517 Size: 696 4741 StyI C'CwwG_G Cuts at: 513 1065 2004 2349 2565 2876 513 Size: 552 939 345 216 311 3074 Fragments arranged by size: 3074 939 552 345 311 216 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2219 3464 3481 3634 3819 4264 325 Size: 1255 639 1245 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1245 639 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3683 5395 96 Size: 274 80 33 51 1443 1706 1712 138 Fragments arranged by size: 1712 1706 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5397 5429 5397 Size: 32 5405 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5437 XbaI T'CTAG_A Cuts at: 2773 2773 Size: 5437 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5437 XmaI C'CCGG_G Cuts at: 1870 2762 1870 Size: 892 4545 XmnI GAAnn'nnTTC Cuts at: 3566 3566 Size: 5437 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3245 276 Size: 53 83 186 936 1711 2468 Fragments arranged by size: 2468 1711 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI