################################## # NO CUT ENZYMES ( 175 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII BalI BamHI BanI BanII BbeI BbrPI BcgI BciVI BclI BfrI BglI BglII BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MseI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AfaI 391 GTAC GT^AC AlwI 486 GATCC GGATC(4/5) AvaI 76 CCCGGG C^[CT]CG[GA]G AvrII 547 CCTAGG C^CTAGG BbsI 196 GAAGAC GAAGAC(2/6) BbvCI 129 CCTCAGC CCTCAGC(-5/-2) BfaI 548 CTAG C^TAG BlnI 547 CCTAGG C^CTAGG BpmI 28 CTGGAG CTGGAG(16/14) BpuAI 196 GAAGAC GAAGAC(2/6) BsoBI 76 CCCGGG C^[CT]CG[GA]G BstEII 66 GGTGACC G^GT[ACGT]ACC BstPI 66 GGTGACC G^GT[ACGT]ACC BstXI 159 CCACTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 485 AGATCC [GA]^GATC[CT] DpnII 486 GATC ^GATC DraII 543 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO109I 543 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 66 GGTGACC G^GT[ACGT]ACC HindIII 406 AAGCTT A^AGCTT MaeI 548 CTAG C^TAG MboI 486 GATC ^GATC MflI 485 AGATCC [GA]^GATC[CT] NdeII 486 GATC ^GATC PflMI 49 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 391 GTAC GT^AC SapI 144 GCTCTTC GCTCTTC(1/4) Sau3AI 486 GATC ^GATC SmaI 76 CCCGGG CCC^GGG TaqI 180 TCGA T^CGA TthHB8I 180 TCGA T^CGA Van91I 49 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 45 CCAACCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 485 AGATCC [GA]^GATC[CT] XmaCI 76 CCCGGG C^CCGGG XmaI 76 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### BcnI 76 CCCGG CC^[GC]GG 77 CCGGG BseRI 490 CTCCTC GAGGAG(10/8) 22 GAGGAG BsgI 499 CTGCAC GTGCAG(16/14) 445 GTGCAG BsrDI 417 CATTGC GCAATG(2/0) 293 GCAATG BsrFI 63 GCCGGT [GA]^CCGG[CT] 434 ACCGGC BstF5I 33 GGATG GGATG(2/0) 54 GGATG BstNI 172 CCTGG CC^[AT]GG 192 CCAGG Cfr10I 63 GCCGGT [GA]^CCGG[CT] 434 ACCGGC Cfr13I 169 GGCCC G^G[ACGT]CC 544 GGCCC EcoRII 172 CCTGG ^CC[AT]GG 192 CCAGG EcoT14I 237 CCAAGG C^C[AT][AT]GG 547 CCTAGG FokI 33 GGATG GGATG(9/13) 54 GGATG HinfI 213 GACTC G^A[ACGT]TC 281 GACTC MaeIII 67 GTGAC ^GT[ACGT]AC 262 GTGAC MslI 8 CATCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 160 CACTGCTGTG MvaI 172 CCTGG CC^[AT]GG 192 CCAGG NciI 76 CCCGG CC^[GC]GG 77 CCGGG NlaIII 246 CATG CATG^ 442 CATG PleI 213 GACTC GAGTC(4/5) 281 GACTC Sau96I 169 GGCCC G^G[ACGT]CC 544 GGCCC StyI 237 CCAAGG C^C[AT][AT]GG 547 CCTAGG Tsp45I 67 GTGAC ^GT[GC]AC 262 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AluI BbvI BsaJI BsiYI BslI BsrI BssKI Cac8I DdeI EarI Fnu4HI HaeIII HapII HpaII HphI ItaI Ksp632I MboII MnlI MspI MwoI NlaIV ScrFI TseI TspRI