(Circular) (Six-base) MAPSORT of: pFN21AB6132.seq Check: 8170 from: 1 to: 6589 pFN21AB6132 6589 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2525 2525 Size: 6589 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4399 278 Size: 53 83 186 936 2863 2468 Fragments arranged by size: 2863 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2906 3932 1937 Size: 969 1026 4594 Fragments arranged by size: 4594 1026 969 Acc65I G'GTAC_C Cuts at: 3910 3910 Size: 6589 AclI AA'CG_TT Cuts at: 1563 4716 5089 1563 Size: 3153 373 3063 Fragments arranged by size: 3153 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2906 4652 6036 6304 1632 Size: 1274 1746 1384 268 1917 Fragments arranged by size: 1917 1746 1384 1274 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6392 AflIII A'CryG_T Cuts at: 1203 2373 2430 5488 6465 1203 Size: 1170 57 3058 977 1327 Fragments arranged by size: 3058 1327 1170 977 57 AgeI A'CCGG_T Cuts at: 5420 5420 Size: 6589 AhdI GACnn_n'nnGTC Cuts at: 5318 5318 Size: 6589 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6589 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2103 1184 Size: 919 5670 AlwNI CAG_nnn'CTG Cuts at: 2405 2578 4332 5904 2405 Size: 173 1754 1572 3090 Fragments arranged by size: 3090 1754 1572 173 ApaI G_GGCC'C Cuts at: 2044 3734 2044 Size: 1690 4899 ApaLI G'TGCA_C Cuts at: 3161 4646 5802 3161 Size: 1485 1156 3948 Fragments arranged by size: 3948 1485 1156 ApoI r'AATT_y Cuts at: 1493 3898 4124 4276 6385 1493 Size: 2405 226 152 2109 1697 Fragments arranged by size: 2405 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5143 160 Size: 872 4111 1606 Fragments arranged by size: 4111 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6589 AvaI C'yCGr_G Cuts at: 1870 1943 2218 2289 2641 2735 2957 2988 3796 Size: 73 275 71 352 94 222 31 808 Cuts at: 3796 3914 1870 Size: 118 4545 Fragments arranged by size: 4545 808 352 275 222 118 94 73 71 31 BamHI G'GATC_C Cuts at: 3919 4286 3919 Size: 367 6222 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2328 2584 3263 3617 3839 Size: 325 217 627 541 256 679 354 222 Cuts at: 3839 3910 5365 618 Size: 71 1455 1842 Fragments arranged by size: 1842 1455 679 627 541 354 325 256 222 217 71 BanII G_rGCy'C Cuts at: 1391 2013 2044 2227 2261 2268 3102 3375 3629 Size: 622 31 183 34 7 834 273 254 Cuts at: 3629 3734 3766 3908 1391 Size: 105 32 142 4072 Fragments arranged by size: 4072 834 622 273 254 183 142 105 34 32 31 7 BbeI G_GCGC'C Cuts at: 2332 2332 Size: 6589 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3598 3697 928 Size: 971 1699 99 3820 Fragments arranged by size: 3820 1699 971 99 BbvCI CC'TCA_GC Cuts at: 2343 2409 3305 2343 Size: 66 896 5627 Fragments arranged by size: 5627 896 66 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3127 3161 4780 4814 1903 Size: 34 1190 34 1619 34 3678 Fragments arranged by size: 3678 1619 1190 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4481 5691 4481 Size: 1210 5379 BclI T'GATC_A Cuts at: 1548 1548 Size: 6589 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2748 3558 3659 Size: 107 122 71 631 605 1076 810 101 Cuts at: 3659 5200 136 Size: 1541 1525 Fragments arranged by size: 1541 1525 1076 810 631 605 122 107 101 71 BglII A'GATC_T Cuts at: 6584 6584 Size: 6589 BlpI GC'TnA_GC Cuts at: 3101 3788 4006 3101 Size: 687 218 5684 Fragments arranged by size: 5684 687 218 Bme1580I G_kGCm'C Cuts at: 1790 2044 2589 3165 3734 4650 5806 1790 Size: 254 545 576 569 916 1156 2573 Fragments arranged by size: 2573 1156 916 576 569 545 254 BmgBI CAC'GTC Cuts at: 1318 3046 1318 Size: 1728 4861 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4320 5278 442 Size: 416 53 83 399 24 2903 958 1753 Fragments arranged by size: 2903 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6589 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3537 3537 Size: 6589 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2252 2600 2996 3086 3116 3412 3478 3666 Size: 1013 348 396 90 30 296 66 188 Cuts at: 3666 5249 1239 Size: 1583 2579 Fragments arranged by size: 2579 1583 1013 396 348 296 188 90 66 30 Bpu10I CC'TnA_GC Cuts at: 2343 2409 3305 3548 2343 Size: 66 896 243 5384 Fragments arranged by size: 5384 896 243 66 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4714 5579 5877 4714 Size: 865 298 5426 Fragments arranged by size: 5426 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2464 5252 882 Size: 617 965 2788 2219 Fragments arranged by size: 2788 2219 965 617 BsaAI yAC'GTr Cuts at: 493 1204 2374 2556 2803 3271 4380 493 Size: 711 1170 182 247 468 1109 2702 Fragments arranged by size: 2702 1170 1109 711 468 247 182 BsaBI GATnn'nnATC Cuts at: 6583 6583 Size: 6589 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2329 2700 4396 4778 Size: 53 83 186 936 796 371 1696 382 Cuts at: 4778 5413 275 Size: 635 1451 Fragments arranged by size: 1696 1451 936 796 635 382 371 186 83 53 BsaWI w'CCGG_w Cuts at: 3845 5022 5420 5694 5841 6252 3845 Size: 1177 398 274 147 411 4182 Fragments arranged by size: 4182 1177 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3393 3423 3639 3669 1178 Size: 30 165 30 1990 30 216 30 4098 Fragments arranged by size: 4098 1990 216 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3680 3864 1186 Size: 2494 184 3911 Fragments arranged by size: 3911 2494 184 BseYI C'CCAG_C Cuts at: 2833 2841 2894 5792 6288 2833 Size: 8 53 2898 496 3134 Fragments arranged by size: 3134 2898 496 53 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2146 2768 2825 3068 3162 1163 Size: 573 410 622 57 243 94 4590 Fragments arranged by size: 4590 622 573 410 243 94 57 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2215 2752 4039 4800 5828 1540 Size: 275 186 214 537 1287 761 1028 2301 Fragments arranged by size: 2301 1287 1028 761 537 275 214 186 BsiHKAI G_wGCw'C Cuts at: 2227 2268 2802 3102 3165 4650 4735 5806 2227 Size: 41 534 300 63 1485 85 1071 3010 Fragments arranged by size: 3010 1485 1071 534 300 85 63 41 BsmI GAATG_Cn' Cuts at: 4100 4193 4100 Size: 93 6496 BsmBI CGTCTCn'nnnn_ Cuts at: 2691 3396 3712 3873 2691 Size: 705 316 161 5407 Fragments arranged by size: 5407 705 316 161 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2044 2227 2261 2268 2589 2802 Size: 399 223 31 183 34 7 321 213 Cuts at: 2802 3102 3165 3375 3629 3734 3766 3908 4650 Size: 300 63 210 254 105 32 142 742 Cuts at: 4650 4735 5806 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 321 300 254 223 213 210 183 142 105 85 63 34 32 31 7 BspEI T'CCGG_A Cuts at: 3845 6252 3845 Size: 2407 4182 BspHI T'CATG_A Cuts at: 4478 6208 4478 Size: 1730 4859 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2525 2682 3944 844 Size: 1681 157 1262 3489 Fragments arranged by size: 3489 1681 1262 157 BsrBI CCG'CTC Cuts at: 1394 1823 2217 3000 4476 1394 Size: 429 394 783 1476 3507 Fragments arranged by size: 3507 1476 783 429 394 BsrDI GCAATG_nn' Cuts at: 65 1248 5084 5258 65 Size: 1183 3836 174 1396 Fragments arranged by size: 3836 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2023 3821 5233 5420 1700 Size: 110 213 1798 1412 187 2869 Fragments arranged by size: 2869 1798 1412 213 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6589 BssHII G'CGCG_C Cuts at: 1925 2033 1925 Size: 108 6481 BssSI C'ACGA_G Cuts at: 4649 5661 4649 Size: 1012 5577 BstAPI GCAn_nnn'nTGC Cuts at: 794 2405 3169 794 Size: 1611 764 4214 Fragments arranged by size: 4214 1611 764 BstBI TT'CG_AA Cuts at: 5393 5393 Size: 6589 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6589 BstXI CCAn_nnnn'nTGG Cuts at: 2841 3064 3211 2841 Size: 223 147 6219 Fragments arranged by size: 6219 223 147 BstYI r'GATC_y Cuts at: 1972 3919 4286 4671 4688 6140 6226 6238 6584 Size: 1947 367 385 17 1452 86 12 346 Cuts at: 6584 1972 Size: 1977 Fragments arranged by size: 1977 1947 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2907 2907 Size: 6589 BtgI C'CryG_G Cuts at: 513 1065 2004 2045 2746 2793 3430 3586 3663 Size: 552 939 41 701 47 637 156 77 Cuts at: 3663 513 Size: 3439 Fragments arranged by size: 3439 939 701 637 552 156 77 47 41 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3231 3570 487 Size: 683 56 2005 339 3506 Fragments arranged by size: 3506 2005 683 339 56 BtsI GCAGTG_nn' Cuts at: 2403 2415 2710 3145 4105 4899 4919 2403 Size: 12 295 435 960 794 20 4073 Fragments arranged by size: 4073 960 794 435 295 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6589 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6554 DraI TTT'AAA Cuts at: 3893 3893 Size: 6589 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5596 809 Size: 507 4280 1802 Fragments arranged by size: 4280 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2749 3433 3651 Size: 54 1425 50 275 18 919 684 218 Cuts at: 3651 3660 3666 4036 4925 6554 8 Size: 9 6 370 889 1629 43 Fragments arranged by size: 1629 1425 919 889 684 370 275 218 54 50 43 18 9 6 EagI C'GGCC_G Cuts at: 1537 1812 2749 4036 1537 Size: 275 937 1287 4090 Fragments arranged by size: 4090 1287 937 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4519 1353 Size: 3166 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2111 2906 3756 5176 5550 5696 Size: 1064 138 522 795 850 1420 374 146 Cuts at: 5696 387 Size: 1280 Fragments arranged by size: 1420 1280 1064 850 795 522 374 146 138 EcoICRI GAG'CTC Cuts at: 2225 2266 3100 2225 Size: 41 834 5714 Fragments arranged by size: 5714 834 41 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 2600 2906 2996 3086 3116 3412 Size: 393 620 348 306 90 90 30 296 Cuts at: 3412 3478 3666 4652 5249 6036 6304 1239 Size: 66 188 986 597 787 268 1524 Fragments arranged by size: 1524 986 787 620 597 393 348 306 296 268 188 90 90 66 30 EcoNI CCTnn'n_nnAGG Cuts at: 2735 2735 Size: 6589 EcoO109I rG'GnC_Cy Cuts at: 2040 2041 3003 3730 3731 2040 Size: 1 962 727 1 4898 Fragments arranged by size: 4898 962 727 1 1 EcoRI G'AATT_C Cuts at: 3898 4276 3898 Size: 378 6211 FspI TGC'GCA Cuts at: 5095 5095 Size: 6589 HaeII r_GCGC'y Cuts at: 1678 2332 2578 3546 3601 5736 1678 Size: 654 246 968 55 2135 2531 Fragments arranged by size: 2531 2135 968 654 246 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3393 3425 3548 3580 3639 Size: 32 135 32 1821 32 123 32 59 Cuts at: 3639 3671 5230 5262 5304 5336 1373 Size: 32 1559 32 42 32 2626 Fragments arranged by size: 2626 1821 1559 135 123 59 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3933 4179 669 Size: 988 281 1995 246 3079 Fragments arranged by size: 3079 1995 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6589 HpaI GTT'AAC Cuts at: 4179 4179 Size: 6589 KasI G'GCGC_C Cuts at: 2328 2328 Size: 6589 KpnI G_GTAC'C Cuts at: 3914 3914 Size: 6589 MfeI C'AATT_G Cuts at: 3464 4188 3464 Size: 724 5865 MluI A'CGCG_T Cuts at: 6465 6465 Size: 6589 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3603 3783 5703 5887 1305 Size: 2298 180 1920 184 2007 Fragments arranged by size: 2298 2007 1920 184 180 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4767 Fragments arranged by size: 4767 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2085 3662 4547 4906 5065 518 Size: 648 919 1577 885 359 159 2042 Fragments arranged by size: 2042 1577 919 885 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2047 2122 2314 2869 3362 3390 3432 3456 Size: 441 75 192 555 493 28 42 24 Cuts at: 3456 3748 3867 4005 4682 5830 6075 1606 Size: 292 119 138 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 555 493 441 292 245 192 138 119 75 42 28 24 NarI GG'CG_CC Cuts at: 2329 2329 Size: 6589 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5098 Fragments arranged by size: 5098 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6589 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6589 NotI GC'GGCC_GC Cuts at: 4036 4036 Size: 6589 NspI r_CATG'y Cuts at: 1132 1229 3947 5492 1132 Size: 97 2718 1545 2229 Fragments arranged by size: 2718 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2323 3211 1348 Size: 975 888 4726 Fragments arranged by size: 4726 975 888 PciI A'CATG_T Cuts at: 5488 5488 Size: 6589 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6589 PfoI T'CCnGG_A Cuts at: 2631 2631 Size: 6589 PmeI GTTT'AAAC Cuts at: 3893 3893 Size: 6589 PmlI CAC'GTG Cuts at: 2556 2803 2556 Size: 247 6342 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4658 4690 6171 6203 4658 Size: 32 1481 32 5044 Fragments arranged by size: 5044 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3003 3003 Size: 6589 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6589 PsiI TTA'TAA Cuts at: 4159 4159 Size: 6589 PspOMI G'GGCC_C Cuts at: 2040 3730 2040 Size: 1690 4899 PstI C_TGCA'G Cuts at: 830 3017 3941 830 Size: 2187 924 3478 Fragments arranged by size: 3478 2187 924 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6589 PvuII CAG'CTG Cuts at: 2314 2869 3390 3456 2314 Size: 555 521 66 5447 Fragments arranged by size: 5447 555 521 66 RsrII CG'GwC_CG Cuts at: 2629 2629 Size: 6589 SacI G_AGCT'C Cuts at: 2227 2268 3102 2227 Size: 41 834 5714 Fragments arranged by size: 5714 834 41 SacII CC_GC'GG Cuts at: 2048 3433 2048 Size: 1385 5204 SalI G'TCGA_C Cuts at: 1936 3931 1936 Size: 1995 4594 SbfI CC_TGCA'GG Cuts at: 3941 3941 Size: 6589 ScaI AGT'ACT Cuts at: 4837 6549 4837 Size: 1712 4877 SfcI C'TryA_G Cuts at: 826 1045 2725 3013 3937 5072 5753 5944 826 Size: 219 1680 288 924 1135 681 191 1471 Fragments arranged by size: 1680 1471 1135 924 681 288 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2748 3659 2748 Size: 911 5678 SfoI GGC'GCC Cuts at: 2330 2330 Size: 6589 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6589 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6589 SmaI CCC'GGG Cuts at: 1872 2291 2990 3916 1872 Size: 419 699 926 4545 Fragments arranged by size: 4545 926 699 419 SmlI C'TyrA_G Cuts at: 820 1017 1943 2957 4693 5594 5856 820 Size: 197 926 1014 1736 901 262 1553 Fragments arranged by size: 1736 1553 1014 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3271 4380 493 Size: 2778 1109 2702 Fragments arranged by size: 2778 2702 1109 SpeI A'CTAG_T Cuts at: 152 152 Size: 6589 SphI G_CATG'C Cuts at: 1132 3947 1132 Size: 2815 3774 SspI AAT'ATT Cuts at: 5 52 4513 5 Size: 47 4461 2081 Fragments arranged by size: 4461 2081 47 StuI AGG'CCT Cuts at: 1517 2639 3281 1517 Size: 1122 642 4825 Fragments arranged by size: 4825 1122 642 StyI C'CwwG_G Cuts at: 513 1065 2004 3195 3337 3375 4028 513 Size: 552 939 1191 142 38 653 3074 Fragments arranged by size: 3074 1191 939 653 552 142 38 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2097 2709 2803 3329 3499 4616 4633 Size: 1255 517 612 94 526 170 1117 17 Cuts at: 4633 4786 4971 5416 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1117 612 526 517 445 185 170 153 94 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4835 6547 96 Size: 274 80 33 51 1443 2858 1712 138 Fragments arranged by size: 2858 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6549 6581 6549 Size: 32 6557 Tth111I GACn'n_nGTC Cuts at: 1316 2479 2861 3407 1316 Size: 1163 382 546 4498 Fragments arranged by size: 4498 1163 546 382 XbaI T'CTAG_A Cuts at: 3925 3925 Size: 6589 XhoI C'TCGA_G Cuts at: 1943 2957 1943 Size: 1014 5575 XmaI C'CCGG_G Cuts at: 1870 2289 2988 3914 1870 Size: 419 699 926 4545 Fragments arranged by size: 4545 926 699 419 XmnI GAAnn'nnTTC Cuts at: 4718 4718 Size: 6589 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4397 276 Size: 53 83 186 936 2863 2468 Fragments arranged by size: 2863 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI SanDI SapI SexAI SrfI SwaI XcmI